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The map label for this gene is pbpF [H]

Identifier: 28572863

GI number: 28572863

Start: 815134

End: 817764

Strand: Direct

Name: pbpF [H]

Synonym: TW722

Alternate gene names: 28572863

Gene position: 815134-817764 (Clockwise)

Preceding gene: 28572851

Following gene: 28572864

Centisome position: 88.03

GC content: 44.81

Gene sequence:

>2631_bases
ATGCTCAAACACTGGCTTAGACTTCTTTGGATTTGTACTATCTCCGGGGTTGTACTTGCGTCAGGAATGGTACCGGCACT
AGCTTTCGCGACCTTTGCGATCAGCAGAGAAGTTAACAAGAGTCTCCCATCCTTTCTTGATGCAGACGATTTATCTCAGA
AATCTGTTATTTATGGGATAAATCGCCAAACTGGCAAACCCGTGGAGATTGCAAGTTTCTATACTCAAAACCGCATTGAC
GTGTCGCTTGATGAAATTTCACTCTTCGTAAGAGACGCGGCAATTGCTGTGGAAGATCCCCGTTTTTACACTAATCACGG
GGTTGATTTAACCGGCTCATTCAGGGCGTTGTTATCAAACCTCCTGAGTGGAGGTGGCTCGATTCAAGGTGGCTCGTCCA
TTACGCAACAATATGTGAAGAATATGCTCCTTCAGAGGTGTGAAATTATATCGCCAGTAAAACCATCTAAGCCCGAGGTA
AAAAGTTCCGAAAGGGACAAAGAGATATACGCACGAGCTTTGAAAAAATACAATACTGATGTTGCTCGGCGCTCTAAGTG
TATACGTGATGTTGTTGGCGTAAGCCTACAGAGAAAAATACGTGAAGTATTTCTTGCATTGCAGGTAAATAGGAATCTAA
GTAAAAATACTGTTCTTAGAAATTACCTTAACATCGTGAATTATGGAGGCTCCATTTATGGAATAGAGGCGGCCAGTAGG
TACTATTTCAGTAAGCATGCCAAGGATTTAACCCTTCCCGAGGCAGCAACACTGATTGGCATAATAAATAGCCCCTCCGT
CCTCCGTATAGATAGGCCAGATAATCCGAATAATGGCAAAAAAAATGGTTATCGGCAAGCAAAGGCTCGCCGAGACTACG
TCTTAAAAAGGATGCTTGTTATCGGAAAGATAACAGAAGATCAGTATAAGAAAGCTGAGAAAACCCAGATTGAACCTCGT
ATAACTTACCCAGAGTACGGATGTGCACCGGCGGGTAACGCTGCATTTTTCTGTGATTATATCCAACATCTTATTCTGGA
TAATAGTGCATTCGGCAAAACCCGTGCAGAACGTAAATTCAGTCTGATGCGTGGTGGATATCGGATATATACAACCCTGG
ATATTGCCCTACAAAATAAAGCTGCAGAAGCTATGAGTAATTATTTTCCCTCATTTGTAAGGGGTCTTTCTGCGGGTGCC
GCACTGGTAACCGTTGAGCCGGGGACAGGTAACCTTCTTGCAATGGTTCAGAATACAAAATACAGTCAGGACCCCGCTGT
TCTCGAAGACCCGAGCTACAGTGCGGTAAACCTGAGTACGGACTTTGATTACGGTGGATCAAGCGGATTTCAAGTAGGGT
CGACCTACAAATTATTTACCCTTATTGCTTGGCTTAGAGCTGGAAAGAGTCTGAATACGGTGGTTGATTCTGCATACGAC
AGAAACAAACAGTCCAAAGCGTTTTCGCTCGGAGATTTCAAAAATACCTGCACAGACCCCGGGGCTCTCAAGCCATACAG
GGGAGCATATGAAGTGAAAAACGCAGCTGGGACAAAAAATTTTACTGTAATGAATGCCACCAAACAGTCTGTTAACACCG
CCTACATGGCAATGGCGCTTCAGCTTGATCTTTGCGACGTTAAGAAAGCAGCCTTGGATACGGGGCTTGCGCCGGCGAAC
ATAGCTTCCGGGGAGGATCTTATTTCATCGCCTTCATCCATTCTTGGCATAAATCAAATTTCTCCACTCAAAATGGCTGA
GGCTTATGCGACAATTGCAGCAGGGGGACGACATTGTCCTACAAGAGCAATAATAAAAATTCTCGATAGACATGGTAAAG
AGGTGCCAATACCGAGGACTGACTGCAAAGACGTGATATCGCAAGACGTCAATGCAGCAACTGCATGGGCATTAAAACAA
GTAATAACCGGCGGCACGGCAACTCAGGCTAATCCGCGTGATGGTATTGAACATATAGCAAAGACAGGCACAACAGACAA
TGCAGCTGATTCATGGATCATAGGGGGCAGCACAAAGGCCGTTACAGCAATATGGACCGGTGTAACAACAAACCGAGCAA
ACTTCTCGCAATACGGTAGGTACCTGAGAGGGAAAGCAGGTGTGTACCCTTTCTATAACGTCAGATATCTTATTTGGCGG
GAGGTTATGCGTTACGCAGATTCCTTATATCCGGGGTCAAGATTTCCAGCTCCTGACCCAAAGTATCTGGTTATGACAAA
CGTAATAGTTCCAAACGTAATAGGTATGACCAAAGAAGAAGCGAAACTTGCATTAGAGCAAATCGGGTTGGTATACGAGG
AAAAGCCTGGGGAGGAGGTCACAAATGATCAGACTGTGACCAATACAGATCCATCGCCTGGCAGTTCTGTCCCTCAGGGC
ACAAGCGTTACCGTAATGTATGCATCAGATCAGTCATTTACCATTCCAAACGTAATAGGTATGACCAAAGAAGAAGCGGT
ACGCTTGCTCTCGGGGCAGAAGCTAAATATAAGCGAAATAAAGGTTTCTAGCCCAACCTTGGCAGGCAAGGTAATCTCTG
TTCTTCCCGGGGTTGGCACATCAATTAGCTCTAATTCAGTGATAACAATAACTGTTGGTAAGCGAGAATAA

Upstream 100 bases:

>100_bases
ACTCACGCTCCCATCGTGTTCTACTGAAATAAGTTTTATAGAATCTAGGTATGGCCAAACTGGATTGCTTCCGTCGTGCT
GGATAATTTTGTAAGAGAAA

Downstream 100 bases:

>100_bases
TGGCGTGATACCTGTGCTATTTGTGTTGTTCGGGCAAGATTTCAGGGTTTATAGGATGCTTGTGTGCCGTAAGTATCCGC
AGACTAAAATAGTCAATGAA

Product: penicillin-binding protein/transpeptidase

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 876; Mature: 876

Protein sequence:

>876_residues
MLKHWLRLLWICTISGVVLASGMVPALAFATFAISREVNKSLPSFLDADDLSQKSVIYGINRQTGKPVEIASFYTQNRID
VSLDEISLFVRDAAIAVEDPRFYTNHGVDLTGSFRALLSNLLSGGGSIQGGSSITQQYVKNMLLQRCEIISPVKPSKPEV
KSSERDKEIYARALKKYNTDVARRSKCIRDVVGVSLQRKIREVFLALQVNRNLSKNTVLRNYLNIVNYGGSIYGIEAASR
YYFSKHAKDLTLPEAATLIGIINSPSVLRIDRPDNPNNGKKNGYRQAKARRDYVLKRMLVIGKITEDQYKKAEKTQIEPR
ITYPEYGCAPAGNAAFFCDYIQHLILDNSAFGKTRAERKFSLMRGGYRIYTTLDIALQNKAAEAMSNYFPSFVRGLSAGA
ALVTVEPGTGNLLAMVQNTKYSQDPAVLEDPSYSAVNLSTDFDYGGSSGFQVGSTYKLFTLIAWLRAGKSLNTVVDSAYD
RNKQSKAFSLGDFKNTCTDPGALKPYRGAYEVKNAAGTKNFTVMNATKQSVNTAYMAMALQLDLCDVKKAALDTGLAPAN
IASGEDLISSPSSILGINQISPLKMAEAYATIAAGGRHCPTRAIIKILDRHGKEVPIPRTDCKDVISQDVNAATAWALKQ
VITGGTATQANPRDGIEHIAKTGTTDNAADSWIIGGSTKAVTAIWTGVTTNRANFSQYGRYLRGKAGVYPFYNVRYLIWR
EVMRYADSLYPGSRFPAPDPKYLVMTNVIVPNVIGMTKEEAKLALEQIGLVYEEKPGEEVTNDQTVTNTDPSPGSSVPQG
TSVTVMYASDQSFTIPNVIGMTKEEAVRLLSGQKLNISEIKVSSPTLAGKVISVLPGVGTSISSNSVITITVGKRE

Sequences:

>Translated_876_residues
MLKHWLRLLWICTISGVVLASGMVPALAFATFAISREVNKSLPSFLDADDLSQKSVIYGINRQTGKPVEIASFYTQNRID
VSLDEISLFVRDAAIAVEDPRFYTNHGVDLTGSFRALLSNLLSGGGSIQGGSSITQQYVKNMLLQRCEIISPVKPSKPEV
KSSERDKEIYARALKKYNTDVARRSKCIRDVVGVSLQRKIREVFLALQVNRNLSKNTVLRNYLNIVNYGGSIYGIEAASR
YYFSKHAKDLTLPEAATLIGIINSPSVLRIDRPDNPNNGKKNGYRQAKARRDYVLKRMLVIGKITEDQYKKAEKTQIEPR
ITYPEYGCAPAGNAAFFCDYIQHLILDNSAFGKTRAERKFSLMRGGYRIYTTLDIALQNKAAEAMSNYFPSFVRGLSAGA
ALVTVEPGTGNLLAMVQNTKYSQDPAVLEDPSYSAVNLSTDFDYGGSSGFQVGSTYKLFTLIAWLRAGKSLNTVVDSAYD
RNKQSKAFSLGDFKNTCTDPGALKPYRGAYEVKNAAGTKNFTVMNATKQSVNTAYMAMALQLDLCDVKKAALDTGLAPAN
IASGEDLISSPSSILGINQISPLKMAEAYATIAAGGRHCPTRAIIKILDRHGKEVPIPRTDCKDVISQDVNAATAWALKQ
VITGGTATQANPRDGIEHIAKTGTTDNAADSWIIGGSTKAVTAIWTGVTTNRANFSQYGRYLRGKAGVYPFYNVRYLIWR
EVMRYADSLYPGSRFPAPDPKYLVMTNVIVPNVIGMTKEEAKLALEQIGLVYEEKPGEEVTNDQTVTNTDPSPGSSVPQG
TSVTVMYASDQSFTIPNVIGMTKEEAVRLLSGQKLNISEIKVSSPTLAGKVISVLPGVGTSISSNSVITITVGKRE
>Mature_876_residues
MLKHWLRLLWICTISGVVLASGMVPALAFATFAISREVNKSLPSFLDADDLSQKSVIYGINRQTGKPVEIASFYTQNRID
VSLDEISLFVRDAAIAVEDPRFYTNHGVDLTGSFRALLSNLLSGGGSIQGGSSITQQYVKNMLLQRCEIISPVKPSKPEV
KSSERDKEIYARALKKYNTDVARRSKCIRDVVGVSLQRKIREVFLALQVNRNLSKNTVLRNYLNIVNYGGSIYGIEAASR
YYFSKHAKDLTLPEAATLIGIINSPSVLRIDRPDNPNNGKKNGYRQAKARRDYVLKRMLVIGKITEDQYKKAEKTQIEPR
ITYPEYGCAPAGNAAFFCDYIQHLILDNSAFGKTRAERKFSLMRGGYRIYTTLDIALQNKAAEAMSNYFPSFVRGLSAGA
ALVTVEPGTGNLLAMVQNTKYSQDPAVLEDPSYSAVNLSTDFDYGGSSGFQVGSTYKLFTLIAWLRAGKSLNTVVDSAYD
RNKQSKAFSLGDFKNTCTDPGALKPYRGAYEVKNAAGTKNFTVMNATKQSVNTAYMAMALQLDLCDVKKAALDTGLAPAN
IASGEDLISSPSSILGINQISPLKMAEAYATIAAGGRHCPTRAIIKILDRHGKEVPIPRTDCKDVISQDVNAATAWALKQ
VITGGTATQANPRDGIEHIAKTGTTDNAADSWIIGGSTKAVTAIWTGVTTNRANFSQYGRYLRGKAGVYPFYNVRYLIWR
EVMRYADSLYPGSRFPAPDPKYLVMTNVIVPNVIGMTKEEAKLALEQIGLVYEEKPGEEVTNDQTVTNTDPSPGSSVPQG
TSVTVMYASDQSFTIPNVIGMTKEEAVRLLSGQKLNISEIKVSSPTLAGKVISVLPGVGTSISSNSVITITVGKRE

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=387, Percent_Identity=29.7157622739018, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1786343, Length=658, Percent_Identity=24.9240121580547, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1788867, Length=314, Percent_Identity=27.7070063694268, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 95601; Mature: 95601

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHWLRLLWICTISGVVLASGMVPALAFATFAISREVNKSLPSFLDADDLSQKSVIYGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
NRQTGKPVEIASFYTQNRIDVSLDEISLFVRDAAIAVEDPRFYTNHGVDLTGSFRALLSN
CCCCCCCEEHHHHHHCCCCEEEHHHHHHHHHHCEEEEECCCEECCCCCCCCHHHHHHHHH
LLSGGGSIQGGSSITQQYVKNMLLQRCEIISPVKPSKPEVKSSERDKEIYARALKKYNTD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHH
VARRSKCIRDVVGVSLQRKIREVFLALQVNRNLSKNTVLRNYLNIVNYGGSIYGIEAASR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHH
YYFSKHAKDLTLPEAATLIGIINSPSVLRIDRPDNPNNGKKNGYRQAKARRDYVLKRMLV
HHHHHCCCCCCCCCHHHEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IGKITEDQYKKAEKTQIEPRITYPEYGCAPAGNAAFFCDYIQHLILDNSAFGKTRAERKF
HHCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
SLMRGGYRIYTTLDIALQNKAAEAMSNYFPSFVRGLSAGAALVTVEPGTGNLLAMVQNTK
HHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCC
YSQDPAVLEDPSYSAVNLSTDFDYGGSSGFQVGSTYKLFTLIAWLRAGKSLNTVVDSAYD
CCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RNKQSKAFSLGDFKNTCTDPGALKPYRGAYEVKNAAGTKNFTVMNATKQSVNTAYMAMAL
CCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
QLDLCDVKKAALDTGLAPANIASGEDLISSPSSILGINQISPLKMAEAYATIAAGGRHCP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHEECCCCCCHHHHHHHHHHHCCCCCCC
TRAIIKILDRHGKEVPIPRTDCKDVISQDVNAATAWALKQVITGGTATQANPRDGIEHIA
HHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KTGTTDNAADSWIIGGSTKAVTAIWTGVTTNRANFSQYGRYLRGKAGVYPFYNVRYLIWR
HCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
EVMRYADSLYPGSRFPAPDPKYLVMTNVIVPNVIGMTKEEAKLALEQIGLVYEEKPGEEV
HHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCHHHHHHHHHHHCCCEECCCCCCC
TNDQTVTNTDPSPGSSVPQGTSVTVMYASDQSFTIPNVIGMTKEEAVRLLSGQKLNISEI
CCCCEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHCCCCCCHHEE
KVSSPTLAGKVISVLPGVGTSISSNSVITITVGKRE
EECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MLKHWLRLLWICTISGVVLASGMVPALAFATFAISREVNKSLPSFLDADDLSQKSVIYGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
NRQTGKPVEIASFYTQNRIDVSLDEISLFVRDAAIAVEDPRFYTNHGVDLTGSFRALLSN
CCCCCCCEEHHHHHHCCCCEEEHHHHHHHHHHCEEEEECCCEECCCCCCCCHHHHHHHHH
LLSGGGSIQGGSSITQQYVKNMLLQRCEIISPVKPSKPEVKSSERDKEIYARALKKYNTD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHH
VARRSKCIRDVVGVSLQRKIREVFLALQVNRNLSKNTVLRNYLNIVNYGGSIYGIEAASR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHH
YYFSKHAKDLTLPEAATLIGIINSPSVLRIDRPDNPNNGKKNGYRQAKARRDYVLKRMLV
HHHHHCCCCCCCCCHHHEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IGKITEDQYKKAEKTQIEPRITYPEYGCAPAGNAAFFCDYIQHLILDNSAFGKTRAERKF
HHCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
SLMRGGYRIYTTLDIALQNKAAEAMSNYFPSFVRGLSAGAALVTVEPGTGNLLAMVQNTK
HHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCC
YSQDPAVLEDPSYSAVNLSTDFDYGGSSGFQVGSTYKLFTLIAWLRAGKSLNTVVDSAYD
CCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RNKQSKAFSLGDFKNTCTDPGALKPYRGAYEVKNAAGTKNFTVMNATKQSVNTAYMAMAL
CCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
QLDLCDVKKAALDTGLAPANIASGEDLISSPSSILGINQISPLKMAEAYATIAAGGRHCP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHEECCCCCCHHHHHHHHHHHCCCCCCC
TRAIIKILDRHGKEVPIPRTDCKDVISQDVNAATAWALKQVITGGTATQANPRDGIEHIA
HHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KTGTTDNAADSWIIGGSTKAVTAIWTGVTTNRANFSQYGRYLRGKAGVYPFYNVRYLIWR
HCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
EVMRYADSLYPGSRFPAPDPKYLVMTNVIVPNVIGMTKEEAKLALEQIGLVYEEKPGEEV
HHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCHHHHHHHHHHHCCCEECCCCCCC
TNDQTVTNTDPSPGSSVPQGTSVTVMYASDQSFTIPNVIGMTKEEAVRLLSGQKLNISEI
CCCCEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHCCCCCCHHEE
KVSSPTLAGKVISVLPGVGTSISSNSVITITVGKRE
EECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]