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The map label for this gene is greA [H]

Identifier: 28572840

GI number: 28572840

Start: 791384

End: 791872

Strand: Direct

Name: greA [H]

Synonym: TW699

Alternate gene names: 28572840

Gene position: 791384-791872 (Clockwise)

Preceding gene: 28572839

Following gene: 28572844

Centisome position: 85.47

GC content: 50.1

Gene sequence:

>489_bases
ATGCCGAATGATTCTGAAACATGGCTCAGTAAGGAAGCGCATAGTGTGCTTGTTGTTGAACTCGAGGAATTGGTTACAAC
AGGGCGCAAGGAAATTGCAAAACGTATTGAGCAAGCAAGGTCTGAAGGTGACCTAAGGGAAAACGGGGGATATAGCGCTG
CGAAAGAGGAGCAGGCTCGTATTGAGGGAAGAATTCAACAGCTACAGGACCTTCTGAGACACGCCCGTGTGGGTCAAGCT
CCGAAGGCAAGGGGGGTAGTTGAGCCCGGCGTAGTCGTGACAGCCAGAATCGGCGGCTCGGAAAGGCGGTTCCTGCTGGG
GAACAGAGAAATAGCCCATACTTCGTCACTTGCAACCTACAGTGAATCCAGTGCCATCGGGCAAGCAATATTGGGCCTAA
AAGAAGGCTCTGAAATTGAATGTAAGTCACCGACAGGAGCTCTTATAAAGGTAGCCATTTTGAAGGTTGAGACATATGAG
GGTCAATGA

Upstream 100 bases:

>100_bases
GGATGGAAGGTTGTTCCTGTGCTGTCGCCACATATGGAGGTGGGGTTGAGAACCACTAAGGTGGCTCATCATGCAGAAAT
TGAGAGCTGTAGGAGGATAT

Downstream 100 bases:

>100_bases
CATATGAATCCCGTAAAAATCGGTAATTTTGCTGCACACTACTGTTGTCAAGTTGTAACTATTGTCAAGTTATACGGTAC
TGGTGTTTGTTAAAACATTA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MPNDSETWLSKEAHSVLVVELEELVTTGRKEIAKRIEQARSEGDLRENGGYSAAKEEQARIEGRIQQLQDLLRHARVGQA
PKARGVVEPGVVVTARIGGSERRFLLGNREIAHTSSLATYSESSAIGQAILGLKEGSEIECKSPTGALIKVAILKVETYE
GQ

Sequences:

>Translated_162_residues
MPNDSETWLSKEAHSVLVVELEELVTTGRKEIAKRIEQARSEGDLRENGGYSAAKEEQARIEGRIQQLQDLLRHARVGQA
PKARGVVEPGVVVTARIGGSERRFLLGNREIAHTSSLATYSESSAIGQAILGLKEGSEIECKSPTGALIKVAILKVETYE
GQ
>Mature_161_residues
PNDSETWLSKEAHSVLVVELEELVTTGRKEIAKRIEQARSEGDLRENGGYSAAKEEQARIEGRIQQLQDLLRHARVGQAP
KARGVVEPGVVVTARIGGSERRFLLGNREIAHTSSLATYSESSAIGQAILGLKEGSEIECKSPTGALIKVAILKVETYEG
Q

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17606; Mature: 17475

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNDSETWLSKEAHSVLVVELEELVTTGRKEIAKRIEQARSEGDLRENGGYSAAKEEQAR
CCCCCHHHHHHCCCEEHEEEHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
IEGRIQQLQDLLRHARVGQAPKARGVVEPGVVVTARIGGSERRFLLGNREIAHTSSLATY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHH
SESSAIGQAILGLKEGSEIECKSPTGALIKVAILKVETYEGQ
CHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEEECCCC
>Mature Secondary Structure 
PNDSETWLSKEAHSVLVVELEELVTTGRKEIAKRIEQARSEGDLRENGGYSAAKEEQAR
CCCCHHHHHHCCCEEHEEEHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
IEGRIQQLQDLLRHARVGQAPKARGVVEPGVVVTARIGGSERRFLLGNREIAHTSSLATY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHH
SESSAIGQAILGLKEGSEIECKSPTGALIKVAILKVETYEGQ
CHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12692562; 11572948 [H]