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The map label for this gene is pth

Identifier: 28572814

GI number: 28572814

Start: 764591

End: 765151

Strand: Reverse

Name: pth

Synonym: TW673

Alternate gene names: 28572814

Gene position: 765151-764591 (Counterclockwise)

Preceding gene: 28572815

Following gene: 28572813

Centisome position: 82.64

GC content: 46.52

Gene sequence:

>561_bases
TTGTGTGTGTACATAGTGGCTGGCCTTGGTAACCCTGGCCCTTCGTATGACAAGACGCGGCACAATGTTGGCCAGATGGT
TCTTGATATCTTGTCAGGAGGTGCGCGTTTCAGGCGTCACAAAACGAATAATTTTTATCTTTCACTCGGTGACATCTTGC
TCGTCAAACCGGATGCGTATATGAATTTATCGGGTCCCGTTATTGCGAGCCTGATGAAATTTCATTCTGTATCAGACCTA
CTTGTTCTGCATGACGATATTGACCTTCCTCTCGGCACGCTTCGATTCAAGCAAGGCGGGGGTACTGCCGGCCATAGAGG
TCTTAGAAGTATAAGTGATTGCCTTGGTTCTGATTACGCAAGATTAAGGGTTGGTATAGGCAGGCCAGAAAATAACCAAA
GCATCGAAGACTTTGTCTTGTCAAACTTCTCTCCTGTTCAGACAGACATTATCAGCAGAACAATACAGCTGGCCGCAGAG
GCCGTTTTGCATCTGAGAGACAATGGCTTTGTTGGCGTAAAACAATTCATAGCCCAAACCAAATCGCCCTCTCGGACTTA
A

Upstream 100 bases:

>100_bases
CCCTTATAAGGTAGCGAGTTACCCTGTTCGCTACAGTACGTTGCCGATGGTCGGCAGTTACAGGCCGAGGTTTAGGTTTG
TGGCGCTGCATATGTAATGC

Downstream 100 bases:

>100_bases
ATAACGTAACCACCGGTCTTTATTTGCGCACTGTACACTCGAGGGGTCTTGCCAAAAGGAGCCCACTGTACAGTATAACT
GCACGGTCACACTGTCGGCG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MCVYIVAGLGNPGPSYDKTRHNVGQMVLDILSGGARFRRHKTNNFYLSLGDILLVKPDAYMNLSGPVIASLMKFHSVSDL
LVLHDDIDLPLGTLRFKQGGGTAGHRGLRSISDCLGSDYARLRVGIGRPENNQSIEDFVLSNFSPVQTDIISRTIQLAAE
AVLHLRDNGFVGVKQFIAQTKSPSRT

Sequences:

>Translated_186_residues
MCVYIVAGLGNPGPSYDKTRHNVGQMVLDILSGGARFRRHKTNNFYLSLGDILLVKPDAYMNLSGPVIASLMKFHSVSDL
LVLHDDIDLPLGTLRFKQGGGTAGHRGLRSISDCLGSDYARLRVGIGRPENNQSIEDFVLSNFSPVQTDIISRTIQLAAE
AVLHLRDNGFVGVKQFIAQTKSPSRT
>Mature_186_residues
MCVYIVAGLGNPGPSYDKTRHNVGQMVLDILSGGARFRRHKTNNFYLSLGDILLVKPDAYMNLSGPVIASLMKFHSVSDL
LVLHDDIDLPLGTLRFKQGGGTAGHRGLRSISDCLGSDYARLRVGIGRPENNQSIEDFVLSNFSPVQTDIISRTIQLAAE
AVLHLRDNGFVGVKQFIAQTKSPSRT

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=178, Percent_Identity=37.0786516853933, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1787455, Length=170, Percent_Identity=36.4705882352941, Blast_Score=94, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321983, Length=178, Percent_Identity=27.5280898876405, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_TROW8 (Q83HD8)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789594.1
- ProteinModelPortal:   Q83HD8
- SMR:   Q83HD8
- STRING:   Q83HD8
- GeneID:   1064873
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW673
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   FSPKHNA
- PhylomeDB:   Q83HD8
- ProtClustDB:   PRK05426
- BioCyc:   TWHI218496:TW0639-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20310; Mature: 20310

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVYIVAGLGNPGPSYDKTRHNVGQMVLDILSGGARFRRHKTNNFYLSLGDILLVKPDAY
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCEEEECCCCC
MNLSGPVIASLMKFHSVSDLLVLHDDIDLPLGTLRFKQGGGTAGHRGLRSISDCLGSDYA
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCE
RLRVGIGRPENNQSIEDFVLSNFSPVQTDIISRTIQLAAEAVLHLRDNGFVGVKQFIAQT
EEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
KSPSRT
CCCCCC
>Mature Secondary Structure
MCVYIVAGLGNPGPSYDKTRHNVGQMVLDILSGGARFRRHKTNNFYLSLGDILLVKPDAY
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCEEEECCCCC
MNLSGPVIASLMKFHSVSDLLVLHDDIDLPLGTLRFKQGGGTAGHRGLRSISDCLGSDYA
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCE
RLRVGIGRPENNQSIEDFVLSNFSPVQTDIISRTIQLAAEAVLHLRDNGFVGVKQFIAQT
EEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
KSPSRT
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174