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The map label for this gene is rsmD [H]

Identifier: 28572799

GI number: 28572799

Start: 749768

End: 750331

Strand: Reverse

Name: rsmD [H]

Synonym: TW656

Alternate gene names: 28572799

Gene position: 750331-749768 (Counterclockwise)

Preceding gene: 28572800

Following gene: 28572798

Centisome position: 81.03

GC content: 47.34

Gene sequence:

>564_bases
ATGAGAATTATTGCGGGGTTTGCAAAATCGATTAAGCTTGAGGCGCCAACCCGTGGAATACGCCCTACAACCGAGAGGGT
GCGAGAGTCACTTATGTCAAGCCTTCAGGCAAGAATGCTGCTTGAAGACGCGGTTGTTATTGATTGCTTCGCTGGAACTG
GTGCCCTTGGTTTAGAAGCCGTAAGCCGTGGCGCACGCGTTTTACACTTAATTGACAAATCCGCAAAAGCCTTCAAGGCT
TGCAAGAAAAACGCCGATCGTGTCAAGGCATTTTGTGAGCATGCACAAATACATGTACACAAAGCAAATGTCACCACATT
TCTCAAATCATCTCTGCAGCACACTACCCGCGCAAGTTTGGTGTTTTTGGACCCTCCGTATGACTATCCACCACACAAAT
TGGAAGAGGTGTTTATTCATCTCTTGCATTGGATTAACGAGGATACAGTTGTAATACTCGAGACTGATCGGGCAAATGGC
ACACCACCTGTCGGAGATGCTCTAAAGGTAACCGAACAAAAACAATACGGCCACAGCGTTGTCTGGTGGATGTCACAGCC
CTGA

Upstream 100 bases:

>100_bases
ACACATTTACCGGGTTATACATAACATGTAACATCTGCACAACTTGGTTTACCTAACTACAGTTTCCAACTGGCCCAGCA
CGATACACTGAACTACCGTA

Downstream 100 bases:

>100_bases
GGCTTGATCACTTATTACAAAAGCTCACAAAACATGTGTGTAACTTCATAGCGGATGTAATAAACTTGCAAGGTGAATCA
CGGGGTGTATCAGAATCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MRIIAGFAKSIKLEAPTRGIRPTTERVRESLMSSLQARMLLEDAVVIDCFAGTGALGLEAVSRGARVLHLIDKSAKAFKA
CKKNADRVKAFCEHAQIHVHKANVTTFLKSSLQHTTRASLVFLDPPYDYPPHKLEEVFIHLLHWINEDTVVILETDRANG
TPPVGDALKVTEQKQYGHSVVWWMSQP

Sequences:

>Translated_187_residues
MRIIAGFAKSIKLEAPTRGIRPTTERVRESLMSSLQARMLLEDAVVIDCFAGTGALGLEAVSRGARVLHLIDKSAKAFKA
CKKNADRVKAFCEHAQIHVHKANVTTFLKSSLQHTTRASLVFLDPPYDYPPHKLEEVFIHLLHWINEDTVVILETDRANG
TPPVGDALKVTEQKQYGHSVVWWMSQP
>Mature_187_residues
MRIIAGFAKSIKLEAPTRGIRPTTERVRESLMSSLQARMLLEDAVVIDCFAGTGALGLEAVSRGARVLHLIDKSAKAFKA
CKKNADRVKAFCEHAQIHVHKANVTTFLKSSLQHTTRASLVFLDPPYDYPPHKLEEVFIHLLHWINEDTVVILETDRANG
TPPVGDALKVTEQKQYGHSVVWWMSQP

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=168, Percent_Identity=37.5, Blast_Score=90, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20894; Mature: 20894

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIAGFAKSIKLEAPTRGIRPTTERVRESLMSSLQARMLLEDAVVIDCFAGTGALGLEA
CCEECCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHH
VSRGARVLHLIDKSAKAFKACKKNADRVKAFCEHAQIHVHKANVTTFLKSSLQHTTRASL
HHCCHHEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHCEE
VFLDPPYDYPPHKLEEVFIHLLHWINEDTVVILETDRANGTPPVGDALKVTEQKQYGHSV
EEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEE
VWWMSQP
EEEECCC
>Mature Secondary Structure
MRIIAGFAKSIKLEAPTRGIRPTTERVRESLMSSLQARMLLEDAVVIDCFAGTGALGLEA
CCEECCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHH
VSRGARVLHLIDKSAKAFKACKKNADRVKAFCEHAQIHVHKANVTTFLKSSLQHTTRASL
HHCCHHEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHCEE
VFLDPPYDYPPHKLEEVFIHLLHWINEDTVVILETDRANGTPPVGDALKVTEQKQYGHSV
EEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEE
VWWMSQP
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]