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The map label for this gene is glyA [H]

Identifier: 28572798

GI number: 28572798

Start: 748409

End: 749695

Strand: Reverse

Name: glyA [H]

Synonym: TW655

Alternate gene names: 28572798

Gene position: 749695-748409 (Counterclockwise)

Preceding gene: 28572799

Following gene: 28572797

Centisome position: 80.97

GC content: 48.17

Gene sequence:

>1287_bases
GTGAATCACGGGGTGTATCAGAATCTTTTCATCGATTCTTTGGATTCTGTTGATCCACAAATCGCCGAAGTGCTTGATCT
CGAACTCAGGCGACAGAGGGATTTTCTGGAGATGATCGCGAGTGAAAATTTTGTTCCACGCGCAATTCTGCAGGCACAGG
GCTCTGTTCTAACTAATAAATACGCTGAAGGATATCCGCAAAACCGCTATTATGGGGGATGCGAATGCGTTGACCTTGCT
GAGGATCTGGCAATCAGTCGCGTGCGCGACCTATTTGGTTCTGAGTTTGCAAATGTCCAGCCACACTCTGGATCTACGGC
AAATGCTGCCGCACTGATGGCCCTTACAGAAGTTGGTAGCACGATTATGGGTCTTGAATTGGATCATGGGGGCCATTTAA
CCCATGGCATGCCTTTGAGCTTCTCCGGTAAACACTACAAGGCCGTGACTTATAGGCTTGACCCAAAAACATGTTTAATC
GATATGGACTCAGTCCGCGACCTTGCCCTGAGGCATAGGCCCAGTGTTATTATCGCCGGATGGTCAGCCTATGTACGTCA
TCTTGATTTTGAAGCTTTCAGAAGCATAGCTGATGAAGTTGGTGCCAGGCTGTGGGTTGATATGGCACACTTTGCTGGCC
TTGTAGCGGCAGGACTTTATCCTTCCCCCATACCGTGGGCGGATGTTGTTACCAGTACTACACACAAAACCCTTGCCGGC
CCCCGAGGTGGCTTTATACTCGCCAAAAAAGAGTTTGGTAAAGCGATCAACACGGCTGTTTTTCCGGGGCAGCAAGGTGG
GCCATTAATGCATGTTATCGCAGCAAAGGCAGTTGCCTTCAAGATTGCCGCATCAGAAGAATTTCGTGAGAGACAAAGGA
TTACTATTGAAGCTGCTCGAACCGTAGCCAAGCGAATAGGGGAGGATTACAGGCTACGAGATAGAGGTATCGATATTTTG
ACAGGAGGAACTGATGTCCATATGGTCCTTATTGACATGAGAAAGTCTGACATGGATGGCCTGACAGGACAAAACCTTCT
TCACGAGGTTGGTGTAACGGTTAATCGGAATTCGATGCCTTATGACAAAAGGCCACCAAGAATAACCAGTGGTATTAGGA
TAGGAACACCCGCCCTTGTAACACGTGGGCTTTCCCTGGATGAATTCGATGAGGTTGCCGATATTATCTCTAATGCTTTG
CTCACGATTGATCTTCCGAAGCAAAAACAGAGGGCACTCAGGATAGCTCGGGCACATCCCGTTTACGAAGGCCTAAGGCA
ATATTGA

Upstream 100 bases:

>100_bases
CGTTGTCTGGTGGATGTCACAGCCCTGAGGCTTGATCACTTATTACAAAAGCTCACAAAACATGTGTGTAACTTCATAGC
GGATGTAATAAACTTGCAAG

Downstream 100 bases:

>100_bases
CTTCTGATTTTCTTTACACACCTGCAGTGTTTTGCTTCTGCCTCGCATGTCTCATGCACTGATGATAAAAATGCGCACCC
TAGACTTGCATTATCCCGTA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNHGVYQNLFIDSLDSVDPQIAEVLDLELRRQRDFLEMIASENFVPRAILQAQGSVLTNKYAEGYPQNRYYGGCECVDLA
EDLAISRVRDLFGSEFANVQPHSGSTANAAALMALTEVGSTIMGLELDHGGHLTHGMPLSFSGKHYKAVTYRLDPKTCLI
DMDSVRDLALRHRPSVIIAGWSAYVRHLDFEAFRSIADEVGARLWVDMAHFAGLVAAGLYPSPIPWADVVTSTTHKTLAG
PRGGFILAKKEFGKAINTAVFPGQQGGPLMHVIAAKAVAFKIAASEEFRERQRITIEAARTVAKRIGEDYRLRDRGIDIL
TGGTDVHMVLIDMRKSDMDGLTGQNLLHEVGVTVNRNSMPYDKRPPRITSGIRIGTPALVTRGLSLDEFDEVADIISNAL
LTIDLPKQKQRALRIARAHPVYEGLRQY

Sequences:

>Translated_428_residues
MNHGVYQNLFIDSLDSVDPQIAEVLDLELRRQRDFLEMIASENFVPRAILQAQGSVLTNKYAEGYPQNRYYGGCECVDLA
EDLAISRVRDLFGSEFANVQPHSGSTANAAALMALTEVGSTIMGLELDHGGHLTHGMPLSFSGKHYKAVTYRLDPKTCLI
DMDSVRDLALRHRPSVIIAGWSAYVRHLDFEAFRSIADEVGARLWVDMAHFAGLVAAGLYPSPIPWADVVTSTTHKTLAG
PRGGFILAKKEFGKAINTAVFPGQQGGPLMHVIAAKAVAFKIAASEEFRERQRITIEAARTVAKRIGEDYRLRDRGIDIL
TGGTDVHMVLIDMRKSDMDGLTGQNLLHEVGVTVNRNSMPYDKRPPRITSGIRIGTPALVTRGLSLDEFDEVADIISNAL
LTIDLPKQKQRALRIARAHPVYEGLRQY
>Mature_428_residues
MNHGVYQNLFIDSLDSVDPQIAEVLDLELRRQRDFLEMIASENFVPRAILQAQGSVLTNKYAEGYPQNRYYGGCECVDLA
EDLAISRVRDLFGSEFANVQPHSGSTANAAALMALTEVGSTIMGLELDHGGHLTHGMPLSFSGKHYKAVTYRLDPKTCLI
DMDSVRDLALRHRPSVIIAGWSAYVRHLDFEAFRSIADEVGARLWVDMAHFAGLVAAGLYPSPIPWADVVTSTTHKTLAG
PRGGFILAKKEFGKAINTAVFPGQQGGPLMHVIAAKAVAFKIAASEEFRERQRITIEAARTVAKRIGEDYRLRDRGIDIL
TGGTDVHMVLIDMRKSDMDGLTGQNLLHEVGVTVNRNSMPYDKRPPRITSGIRIGTPALVTRGLSLDEFDEVADIISNAL
LTIDLPKQKQRALRIARAHPVYEGLRQY

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=413, Percent_Identity=44.0677966101695, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI261862350, Length=413, Percent_Identity=44.0677966101695, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI261862348, Length=413, Percent_Identity=44.0677966101695, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI19923315, Length=413, Percent_Identity=44.0677966101695, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI261862346, Length=412, Percent_Identity=43.2038834951456, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI22547186, Length=425, Percent_Identity=41.1764705882353, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI22547189, Length=410, Percent_Identity=38.780487804878, Blast_Score=248, Evalue=8e-66,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=49.5145631067961, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25144732, Length=413, Percent_Identity=43.0992736077482, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI25144729, Length=413, Percent_Identity=43.0992736077482, Blast_Score=332, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6319739, Length=418, Percent_Identity=41.3875598086124, Blast_Score=318, Evalue=9e-88,
Organism=Saccharomyces cerevisiae, GI6323087, Length=432, Percent_Identity=37.5, Blast_Score=300, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24640005, Length=417, Percent_Identity=45.3237410071942, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI221329721, Length=417, Percent_Identity=45.3237410071942, Blast_Score=340, Evalue=2e-93,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 47220; Mature: 47220

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHGVYQNLFIDSLDSVDPQIAEVLDLELRRQRDFLEMIASENFVPRAILQAQGSVLTNK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHH
YAEGYPQNRYYGGCECVDLAEDLAISRVRDLFGSEFANVQPHSGSTANAAALMALTEVGS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
TIMGLELDHGGHLTHGMPLSFSGKHYKAVTYRLDPKTCLIDMDSVRDLALRHRPSVIIAG
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEHHHHHHHHHHCCCCEEEEC
WSAYVRHLDFEAFRSIADEVGARLWVDMAHFAGLVAAGLYPSPIPWADVVTSTTHKTLAG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCC
PRGGFILAKKEFGKAINTAVFPGQQGGPLMHVIAAKAVAFKIAASEEFRERQRITIEAAR
CCCCEEEEHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHH
TVAKRIGEDYRLRDRGIDILTGGTDVHMVLIDMRKSDMDGLTGQNLLHEVGVTVNRNSMP
HHHHHCCCCCEEHHCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHCCEEECCCCC
YDKRPPRITSGIRIGTPALVTRGLSLDEFDEVADIISNALLTIDLPKQKQRALRIARAHP
CCCCCCCCCCCEEECCHHHHHCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCH
VYEGLRQY
HHHHHHCC
>Mature Secondary Structure
MNHGVYQNLFIDSLDSVDPQIAEVLDLELRRQRDFLEMIASENFVPRAILQAQGSVLTNK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHH
YAEGYPQNRYYGGCECVDLAEDLAISRVRDLFGSEFANVQPHSGSTANAAALMALTEVGS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
TIMGLELDHGGHLTHGMPLSFSGKHYKAVTYRLDPKTCLIDMDSVRDLALRHRPSVIIAG
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEHHHHHHHHHHCCCCEEEEC
WSAYVRHLDFEAFRSIADEVGARLWVDMAHFAGLVAAGLYPSPIPWADVVTSTTHKTLAG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCC
PRGGFILAKKEFGKAINTAVFPGQQGGPLMHVIAAKAVAFKIAASEEFRERQRITIEAAR
CCCCEEEEHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHH
TVAKRIGEDYRLRDRGIDILTGGTDVHMVLIDMRKSDMDGLTGQNLLHEVGVTVNRNSMP
HHHHHCCCCCEEHHCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHCCEEECCCCC
YDKRPPRITSGIRIGTPALVTRGLSLDEFDEVADIISNALLTIDLPKQKQRALRIARAHP
CCCCCCCCCCCEEECCHHHHHCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCH
VYEGLRQY
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174 [H]