Click here to switch to the map view.

The map label for this gene is pdhD [H]

Identifier: 28572791

GI number: 28572791

Start: 737310

End: 738668

Strand: Reverse

Name: pdhD [H]

Synonym: TW646

Alternate gene names: 28572791

Gene position: 738668-737310 (Counterclockwise)

Preceding gene: 28572792

Following gene: 28572790

Centisome position: 79.78

GC content: 45.62

Gene sequence:

>1359_bases
TTGGAAGAATACGATCTAATCGTTGTTGGTGCTGGCAGTGGTAACAGTATTCTTGATCAGCGTTTTGATTCCCTTAAAAT
CGCCCTTCTCGATGATGGTGCGCATTTTGGAGGGACATGCCTAAATTACGGGTGTATTCCGACAAAAATGCTTCTCCGTC
CTGCTACCCTGGCGTATCAGGCTAAGCATGCTTCAAAACTTGGGGTTCATTTTTCAGATCCGCGAATTGATTGGCAAAAA
ATCCGCTCTAGGACTTTTTCAACAACTGACGATATTAGCGCAGCGGGTCTCGAATATCGCCTTGGGTGTAGCAATATAGA
TGTTTATCGAGAATCCTATTTTTTTATTGATCAAAGAACGTTGCAAAGTTCCTCTGGACATAGGATTAGTGGGGAGAAAA
TTGTAATCGCAGCGGGCTCAAGGCCTAGGCTGTTTGGAATAGAACCTGATAACCGACTGATCTATACTTCGGCGGAGATT
ATGCGAATTGAGCAATTGCCGGAGTCACTTGTTATTCTTGGCGGCGGAGTTGTTGCGGTCGAAATGGCAACCTTCTTTTC
TGGATATGGTGTTGATACTACAACTGTTAATCGCTCTGAGTTTTTGTTATCTAGCTTCGATACAGAAGTTGCAAAAAGCC
TTACAGCGCAGGTTCCGTGGAAGCATATAGGACAGGAGAGGGTGCAAAATGTTAGCAAAAAAGATAGTTCTGTTGAGATA
GAACTTTCATCGGGCACGCGTCTTTTGGCTCAAGCACTTCTGCTGGCACAGGGCAGGGTGTCCAATGCCGACAAATTGCG
TTGCCGTGATGTTGGATTTGATATTCACAGTGATGGGCGCCTGAAAACTGATAAGTACCAGAGGGTTCTGGCCGACAATC
GCCCGCTTGATAATGTTTTTGCACTAGGTGATGTCTCTGACCCCAATCAGCTAAAGCATATTGCAAACCACCAAGCTCGC
ATTGTTGGGCATAATTTGCTGAATGGAGACATGTTGGCATCTGATCTTGTGCCACCTGCTGCTGCTGTTTTCAGCGAACC
GCAGGTGGCATGGGTTGGTACTTTTCCAAATAATTCAATAGTTGTTAGGGGGAATTATTCAGATACAGCATATGGATGGG
CATTCGGTTTAAGGCCAGATGACGCATTTGTGAAGCTTTTTATTGATAAATCGGGTGTGCTGAATGGTGCTTTTGTAATC
GGCCCAGAGGCAAGTATCATTATCCAGCCGCTTATTCAGGCGGTTAGCCTGAATTTGAAGGTTCAGGGGCTTGCCCGGTC
ACAGTATTGGCCCCATCCGGCCGGGACTGAAGTTGTTGAAAATGCTCTACTCAAAGCTGAAGAATTCCTCCGTGCATAA

Upstream 100 bases:

>100_bases
GTCAATGCGCCAGTCTCCGGGCAGGTTATTCGCATTTCAATTCCACCGGGTCGTCAGGTGGATATTGGCGATCTGATTAT
GGAAATAAGGGTCTGAAACA

Downstream 100 bases:

>100_bases
AAAGGGGTATTGGTTGTATTTTTTCATATGCTTGAGTATCCGTATATATGTGCATCCCACGAAATAATGAGATTGGCTTT
GAGGTACTGATGCATTGCGG

Product: mycothione reductase

Products: NA

Alternate protein names: Mycothiol-disulfide reductase; NADPH-dependent mycothione reductase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MEEYDLIVVGAGSGNSILDQRFDSLKIALLDDGAHFGGTCLNYGCIPTKMLLRPATLAYQAKHASKLGVHFSDPRIDWQK
IRSRTFSTTDDISAAGLEYRLGCSNIDVYRESYFFIDQRTLQSSSGHRISGEKIVIAAGSRPRLFGIEPDNRLIYTSAEI
MRIEQLPESLVILGGGVVAVEMATFFSGYGVDTTTVNRSEFLLSSFDTEVAKSLTAQVPWKHIGQERVQNVSKKDSSVEI
ELSSGTRLLAQALLLAQGRVSNADKLRCRDVGFDIHSDGRLKTDKYQRVLADNRPLDNVFALGDVSDPNQLKHIANHQAR
IVGHNLLNGDMLASDLVPPAAAVFSEPQVAWVGTFPNNSIVVRGNYSDTAYGWAFGLRPDDAFVKLFIDKSGVLNGAFVI
GPEASIIIQPLIQAVSLNLKVQGLARSQYWPHPAGTEVVENALLKAEEFLRA

Sequences:

>Translated_452_residues
MEEYDLIVVGAGSGNSILDQRFDSLKIALLDDGAHFGGTCLNYGCIPTKMLLRPATLAYQAKHASKLGVHFSDPRIDWQK
IRSRTFSTTDDISAAGLEYRLGCSNIDVYRESYFFIDQRTLQSSSGHRISGEKIVIAAGSRPRLFGIEPDNRLIYTSAEI
MRIEQLPESLVILGGGVVAVEMATFFSGYGVDTTTVNRSEFLLSSFDTEVAKSLTAQVPWKHIGQERVQNVSKKDSSVEI
ELSSGTRLLAQALLLAQGRVSNADKLRCRDVGFDIHSDGRLKTDKYQRVLADNRPLDNVFALGDVSDPNQLKHIANHQAR
IVGHNLLNGDMLASDLVPPAAAVFSEPQVAWVGTFPNNSIVVRGNYSDTAYGWAFGLRPDDAFVKLFIDKSGVLNGAFVI
GPEASIIIQPLIQAVSLNLKVQGLARSQYWPHPAGTEVVENALLKAEEFLRA
>Mature_452_residues
MEEYDLIVVGAGSGNSILDQRFDSLKIALLDDGAHFGGTCLNYGCIPTKMLLRPATLAYQAKHASKLGVHFSDPRIDWQK
IRSRTFSTTDDISAAGLEYRLGCSNIDVYRESYFFIDQRTLQSSSGHRISGEKIVIAAGSRPRLFGIEPDNRLIYTSAEI
MRIEQLPESLVILGGGVVAVEMATFFSGYGVDTTTVNRSEFLLSSFDTEVAKSLTAQVPWKHIGQERVQNVSKKDSSVEI
ELSSGTRLLAQALLLAQGRVSNADKLRCRDVGFDIHSDGRLKTDKYQRVLADNRPLDNVFALGDVSDPNQLKHIANHQAR
IVGHNLLNGDMLASDLVPPAAAVFSEPQVAWVGTFPNNSIVVRGNYSDTAYGWAFGLRPDDAFVKLFIDKSGVLNGAFVI
GPEASIIIQPLIQAVSLNLKVQGLARSQYWPHPAGTEVVENALLKAEEFLRA

Specific function: Catalyzes the NAD(P)H-dependent reduction of mycothione (the oxidized disulfide form of mycothiol) to mycothiol [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=27.0042194092827, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=25.1152073732719, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI50301238, Length=360, Percent_Identity=26.3888888888889, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=24.367816091954, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=24.367816091954, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=24.367816091954, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=24.367816091954, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=24.367816091954, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI291045268, Length=317, Percent_Identity=26.813880126183, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI22035672, Length=410, Percent_Identity=25.3658536585366, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=26.2931034482759, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87082354, Length=373, Percent_Identity=27.0777479892761, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789915, Length=337, Percent_Identity=28.1899109792285, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081717, Length=369, Percent_Identity=26.8292682926829, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=25.5274261603376, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.5135699373695, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=363, Percent_Identity=24.7933884297521, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983419, Length=363, Percent_Identity=24.7933884297521, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982272, Length=470, Percent_Identity=23.8297872340426, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=25.4166666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325166, Length=369, Percent_Identity=23.3062330623306, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=23.2217573221757, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358499, Length=474, Percent_Identity=24.8945147679325, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17737741, Length=492, Percent_Identity=25.2032520325203, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24640549, Length=503, Percent_Identity=24.8508946322068, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640553, Length=503, Percent_Identity=24.8508946322068, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640551, Length=503, Percent_Identity=24.8508946322068, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017817
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.15 [H]

Molecular weight: Translated: 49527; Mature: 49527

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEYDLIVVGAGSGNSILDQRFDSLKIALLDDGAHFGGTCLNYGCIPTKMLLRPATLAYQ
CCCEEEEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCHHHHHCCHHHHHH
AKHASKLGVHFSDPRIDWQKIRSRTFSTTDDISAAGLEYRLGCSNIDVYRESYFFIDQRT
HHHHHHCCEEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECEEEEEEHHH
LQSSSGHRISGEKIVIAAGSRPRLFGIEPDNRLIYTSAEIMRIEQLPESLVILGGGVVAV
HCCCCCCEECCCEEEEEECCCCEEEEECCCCCEEEEHHHHHHHHHCCCEEEEECCCEEEE
EMATFFSGYGVDTTTVNRSEFLLSSFDTEVAKSLTAQVPWKHIGQERVQNVSKKDSSVEI
EHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
ELSSGTRLLAQALLLAQGRVSNADKLRCRDVGFDIHSDGRLKTDKYQRVLADNRPLDNVF
EECCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCCHHHHHHHHHCCCCCCCEE
ALGDVSDPNQLKHIANHQARIVGHNLLNGDMLASDLVPPAAAVFSEPQVAWVGTFPNNSI
EECCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCHHHHCCCCCEEEEEECCCCEE
VVRGNYSDTAYGWAFGLRPDDAFVKLFIDKSGVLNGAFVIGPEASIIIQPLIQAVSLNLK
EEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCEE
VQGLARSQYWPHPAGTEVVENALLKAEEFLRA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEYDLIVVGAGSGNSILDQRFDSLKIALLDDGAHFGGTCLNYGCIPTKMLLRPATLAYQ
CCCEEEEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCHHHHHCCHHHHHH
AKHASKLGVHFSDPRIDWQKIRSRTFSTTDDISAAGLEYRLGCSNIDVYRESYFFIDQRT
HHHHHHCCEEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECEEEEEEHHH
LQSSSGHRISGEKIVIAAGSRPRLFGIEPDNRLIYTSAEIMRIEQLPESLVILGGGVVAV
HCCCCCCEECCCEEEEEECCCCEEEEECCCCCEEEEHHHHHHHHHCCCEEEEECCCEEEE
EMATFFSGYGVDTTTVNRSEFLLSSFDTEVAKSLTAQVPWKHIGQERVQNVSKKDSSVEI
EHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
ELSSGTRLLAQALLLAQGRVSNADKLRCRDVGFDIHSDGRLKTDKYQRVLADNRPLDNVF
EECCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCCHHHHHHHHHCCCCCCCEE
ALGDVSDPNQLKHIANHQARIVGHNLLNGDMLASDLVPPAAAVFSEPQVAWVGTFPNNSI
EECCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCHHHHCCCCCEEEEEECCCCEE
VVRGNYSDTAYGWAFGLRPDDAFVKLFIDKSGVLNGAFVIGPEASIIIQPLIQAVSLNLK
EEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCEE
VQGLARSQYWPHPAGTEVVENALLKAEEFLRA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]