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The map label for this gene is miaA [H]

Identifier: 28572772

GI number: 28572772

Start: 712844

End: 713704

Strand: Reverse

Name: miaA [H]

Synonym: TW626

Alternate gene names: 28572772

Gene position: 713704-712844 (Counterclockwise)

Preceding gene: 28572773

Following gene: 28572771

Centisome position: 77.08

GC content: 45.64

Gene sequence:

>861_bases
TTGTTATCACTTGATGCAGCCCGTGACTTCAACGGGCACATAGTGAATGCTGACTCAATGCAGCTTTACCGTGATATGGA
TATTGGGACCGCAAAATTGTCTCACAGCGCCCGACAAGGCATACCACACCATCAGATAGACGTGATTGACCCAAGTAGTG
AAGCCAGTGTGGCCCGCTATAAGCTCTCTGCCCAGACTTGCATTAAGCACATTCACGCCCTCAATAGTATTCCGTTTCTT
GTTGGAGGGTCGGGTTTATATGTCTCATCTGTAGTCCACAATTTGCAATTTCCTCCAACAGATGGCAGAGTCAGAAAGTT
ACTTGAGGACGAGGCTGATAAGAGCGGCATTGGCGTACTACATGACAGGCTGCTAAAGCTCCATCCCGGATTTACAGTTT
CCAGGGGCAACCGAAGGCGGATTATCAGGGCCCTTGAAGTGGCATATATAACGGGCCGTTCCCCCAACCCCGTTCTTCCG
TTACAAAATCGAGCAAATAATTTTATTGTCATAAATCTTATTTGTGATAAAGGAACTTTGGATATCCGTCTTCAAAAACG
CGTTGAGTCATTCTATGACAATGGCCTGATTGATGAGGTAAGACTGCTGCAGGAGAAATACGTCCTTGGCAGAACAGCCG
CTAAGGCAATTGGGTACAAGCAGGCAATTATGTATTTGGCGGGTGAAATAAGCTGCGCGGATGCAAAGGATAGCACCCTT
CAGGAGACTATTAGGCTCGCGAATAAGCAGATCAAGTGGTTTAGACGATATTCCGGACAACACATTGTCGACACAACGGA
TATGTCTGTTGCATATGAACAGATTCGTTCTATTCTCAATAAATCTTTTCGTATATCATGA

Upstream 100 bases:

>100_bases
GAATTCGGTATTACCAGCGCTATATGGATTTGTTTCCTGGTTTTCTATTTCATAAGTGTTAGCTGTAGCTTTTGTAGGCG
CAACCGGTACGGGAAAGAGT

Downstream 100 bases:

>100_bases
CTTTATATTCTGCGTGTGCGTGCAAATGCCTGCTTCTTATTCAAAACTTATGTCACATCCAAAATTTGTCGCTAAAGTGT
CATTCATTATTTATCACACA

Product: tRNA delta (2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLSLDAARDFNGHIVNADSMQLYRDMDIGTAKLSHSARQGIPHHQIDVIDPSSEASVARYKLSAQTCIKHIHALNSIPFL
VGGSGLYVSSVVHNLQFPPTDGRVRKLLEDEADKSGIGVLHDRLLKLHPGFTVSRGNRRRIIRALEVAYITGRSPNPVLP
LQNRANNFIVINLICDKGTLDIRLQKRVESFYDNGLIDEVRLLQEKYVLGRTAAKAIGYKQAIMYLAGEISCADAKDSTL
QETIRLANKQIKWFRRYSGQHIVDTTDMSVAYEQIRSILNKSFRIS

Sequences:

>Translated_286_residues
MLSLDAARDFNGHIVNADSMQLYRDMDIGTAKLSHSARQGIPHHQIDVIDPSSEASVARYKLSAQTCIKHIHALNSIPFL
VGGSGLYVSSVVHNLQFPPTDGRVRKLLEDEADKSGIGVLHDRLLKLHPGFTVSRGNRRRIIRALEVAYITGRSPNPVLP
LQNRANNFIVINLICDKGTLDIRLQKRVESFYDNGLIDEVRLLQEKYVLGRTAAKAIGYKQAIMYLAGEISCADAKDSTL
QETIRLANKQIKWFRRYSGQHIVDTTDMSVAYEQIRSILNKSFRIS
>Mature_286_residues
MLSLDAARDFNGHIVNADSMQLYRDMDIGTAKLSHSARQGIPHHQIDVIDPSSEASVARYKLSAQTCIKHIHALNSIPFL
VGGSGLYVSSVVHNLQFPPTDGRVRKLLEDEADKSGIGVLHDRLLKLHPGFTVSRGNRRRIIRALEVAYITGRSPNPVLP
LQNRANNFIVINLICDKGTLDIRLQKRVESFYDNGLIDEVRLLQEKYVLGRTAAKAIGYKQAIMYLAGEISCADAKDSTL
QETIRLANKQIKWFRRYSGQHIVDTTDMSVAYEQIRSILNKSFRIS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=292, Percent_Identity=28.0821917808219, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1790613, Length=276, Percent_Identity=28.6231884057971, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144712, Length=234, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=27.5510204081633, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=28.5185185185185, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 32080; Mature: 32080

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLDAARDFNGHIVNADSMQLYRDMDIGTAKLSHSARQGIPHHQIDVIDPSSEASVARY
CCCCCCCCCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
KLSAQTCIKHIHALNSIPFLVGGSGLYVSSVVHNLQFPPTDGRVRKLLEDEADKSGIGVL
HHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHH
HDRLLKLHPGFTVSRGNRRRIIRALEVAYITGRSPNPVLPLQNRANNFIVINLICDKGTL
HHHHHHHCCCEEECCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEEEECCCCE
DIRLQKRVESFYDNGLIDEVRLLQEKYVLGRTAAKAIGYKQAIMYLAGEISCADAKDSTL
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHH
QETIRLANKQIKWFRRYSGQHIVDTTDMSVAYEQIRSILNKSFRIS
HHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSLDAARDFNGHIVNADSMQLYRDMDIGTAKLSHSARQGIPHHQIDVIDPSSEASVARY
CCCCCCCCCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
KLSAQTCIKHIHALNSIPFLVGGSGLYVSSVVHNLQFPPTDGRVRKLLEDEADKSGIGVL
HHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHH
HDRLLKLHPGFTVSRGNRRRIIRALEVAYITGRSPNPVLPLQNRANNFIVINLICDKGTL
HHHHHHHCCCEEECCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEEEECCCCE
DIRLQKRVESFYDNGLIDEVRLLQEKYVLGRTAAKAIGYKQAIMYLAGEISCADAKDSTL
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHH
QETIRLANKQIKWFRRYSGQHIVDTTDMSVAYEQIRSILNKSFRIS
HHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]