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The map label for this gene is tgt [H]

Identifier: 28572766

GI number: 28572766

Start: 704564

End: 705778

Strand: Direct

Name: tgt [H]

Synonym: TW620

Alternate gene names: 28572766

Gene position: 704564-705778 (Clockwise)

Preceding gene: 28572748

Following gene: 28572781

Centisome position: 76.09

GC content: 46.01

Gene sequence:

>1215_bases
ATGCTTGACTTACCCGTGTGGGGCAATTATATCACTGGCTGGAGTATAGGGAGATGGAGTATGTTCTCTGTTCTAAAACA
AACAGAGTATGGGAGAACGGGGATAATCAGCACATGTAATGGACAGATACATACACCAGCCTTTATCCCTGTTGCAACCA
AAGCAAGTATAAAGGGTGTTCTGCCAGAACAGATGAAACAATTGGGTGCGCAGGGGCTACTTGCCAATGCGTATCATTTA
TACCTTAGGCCGGGGCCGGATACGATCGCAGCAGCAGGTGGCCTTTCCAGGTTCATGTCATGGAACGGCCCAACTTTCAC
AGACTCAGGCGGTTTCCAAGTGATGAGCCTCGGTCGTGGAGGTAAAAAAGTTATTTCTATGGAGGTGCCGGATAATACAT
GGGAGAGTAATATACGGAAAACAACCCTCATAAGAATTGATGATCAAGGCGTTTGGTTTAGATCACACATAGATGGCTCA
CAACACAGGTTTACGCCTGAAGAGTCCATAAGAGTACAGAACAAGATCGGCGCGGATATAATCTTCGCATTTGACCAACT
AACAACCCTGGATGATTCACGAGCGGTACAGGAATACGCACTTGAGAGAACCAGACTCTGGGCAGAACGGTGCTTAAATC
AGCATTCCAAGGAAGATCAGGCGCTGTTTGCAGTGATACAGGGTGCTAATTACAGAGATCTTCGAGAGAAAGCATGTAGA
GATCTTGGGCAAATGGGATTTGATGGCTTTGGAATAGGTGGGGCATTGGAAAAGTCACGCATAAGGGAAATAATAACCTG
GTGCACAAGAAAATTACCTGAGGAGAAACCGCGACATCTTCTCGGTATATCAGAGCCCAATGATCTGATGAATGCCATTG
AAGCTGGTATAGACACATTTGACTGCGTTTCGCCGACACGTGTTGCAAGGAATACGGCATTCTACACACCTTCCGGGAGG
AAGAATCTGAGGCGTAAAATGTACAGAAACGATTTCTCTCCTCTTGTAGAGGGATGTGCGTGTTATGCGTGTTCGAACTT
CACGCGAGCGTATATACATCATCTCTTTCGGGCAAAAGAAATGTGTGCGGCGACTTTAACCTCTATTCATAATGAGTACT
TTATTGTCAGTATGGTCGACGGGGCAAGGAAGGCCATAGACGAAGGGTACTTCCGGGAATACAAGGGTAAAATCCTGCGC
GAATACTATAAGTAA

Upstream 100 bases:

>100_bases
ATAAAGTGACAAGTGCTTTCTTCACCGTGTAGCTACTCCATTCGTTATTACATTACTTAGTAATCACGTTTACTATTCTT
GTTATTTACGTCTTTTATTT

Downstream 100 bases:

>100_bases
TCGCTACGCTTGTTTGGCAGAAAATTTAGAGTACCACTTGTAAAACTTAGAGTACCACCTATAGATCGCCGTGAATATTC
CAGATACTACAGCAGATACC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLDLPVWGNYITGWSIGRWSMFSVLKQTEYGRTGIISTCNGQIHTPAFIPVATKASIKGVLPEQMKQLGAQGLLANAYHL
YLRPGPDTIAAAGGLSRFMSWNGPTFTDSGGFQVMSLGRGGKKVISMEVPDNTWESNIRKTTLIRIDDQGVWFRSHIDGS
QHRFTPEESIRVQNKIGADIIFAFDQLTTLDDSRAVQEYALERTRLWAERCLNQHSKEDQALFAVIQGANYRDLREKACR
DLGQMGFDGFGIGGALEKSRIREIITWCTRKLPEEKPRHLLGISEPNDLMNAIEAGIDTFDCVSPTRVARNTAFYTPSGR
KNLRRKMYRNDFSPLVEGCACYACSNFTRAYIHHLFRAKEMCAATLTSIHNEYFIVSMVDGARKAIDEGYFREYKGKILR
EYYK

Sequences:

>Translated_404_residues
MLDLPVWGNYITGWSIGRWSMFSVLKQTEYGRTGIISTCNGQIHTPAFIPVATKASIKGVLPEQMKQLGAQGLLANAYHL
YLRPGPDTIAAAGGLSRFMSWNGPTFTDSGGFQVMSLGRGGKKVISMEVPDNTWESNIRKTTLIRIDDQGVWFRSHIDGS
QHRFTPEESIRVQNKIGADIIFAFDQLTTLDDSRAVQEYALERTRLWAERCLNQHSKEDQALFAVIQGANYRDLREKACR
DLGQMGFDGFGIGGALEKSRIREIITWCTRKLPEEKPRHLLGISEPNDLMNAIEAGIDTFDCVSPTRVARNTAFYTPSGR
KNLRRKMYRNDFSPLVEGCACYACSNFTRAYIHHLFRAKEMCAATLTSIHNEYFIVSMVDGARKAIDEGYFREYKGKILR
EYYK
>Mature_404_residues
MLDLPVWGNYITGWSIGRWSMFSVLKQTEYGRTGIISTCNGQIHTPAFIPVATKASIKGVLPEQMKQLGAQGLLANAYHL
YLRPGPDTIAAAGGLSRFMSWNGPTFTDSGGFQVMSLGRGGKKVISMEVPDNTWESNIRKTTLIRIDDQGVWFRSHIDGS
QHRFTPEESIRVQNKIGADIIFAFDQLTTLDDSRAVQEYALERTRLWAERCLNQHSKEDQALFAVIQGANYRDLREKACR
DLGQMGFDGFGIGGALEKSRIREIITWCTRKLPEEKPRHLLGISEPNDLMNAIEAGIDTFDCVSPTRVARNTAFYTPSGR
KNLRRKMYRNDFSPLVEGCACYACSNFTRAYIHHLFRAKEMCAATLTSIHNEYFIVSMVDGARKAIDEGYFREYKGKILR
EYYK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=377, Percent_Identity=34.2175066312997, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI13375872, Length=252, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=37.7717391304348, Blast_Score=268, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17544680, Length=386, Percent_Identity=33.419689119171, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI25146502, Length=189, Percent_Identity=25.9259259259259, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19920510, Length=378, Percent_Identity=35.978835978836, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI21355311, Length=223, Percent_Identity=25.5605381165919, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45704; Mature: 45704

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLPVWGNYITGWSIGRWSMFSVLKQTEYGRTGIISTCNGQIHTPAFIPVATKASIKGV
CCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCEECCEEEEEECCHHHCCC
LPEQMKQLGAQGLLANAYHLYLRPGPDTIAAAGGLSRFMSWNGPTFTDSGGFQVMSLGRG
CHHHHHHHCCCCEEECEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
GKKVISMEVPDNTWESNIRKTTLIRIDDQGVWFRSHIDGSQHRFTPEESIRVQNKIGADI
CCEEEEEECCCCCHHHCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCHH
IFAFDQLTTLDDSRAVQEYALERTRLWAERCLNQHSKEDQALFAVIQGANYRDLREKACR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHH
DLGQMGFDGFGIGGALEKSRIREIITWCTRKLPEEKPRHLLGISEPNDLMNAIEAGIDTF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCH
DCVSPTRVARNTAFYTPSGRKNLRRKMYRNDFSPLVEGCACYACSNFTRAYIHHLFRAKE
HCCCHHHHHHCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MCAATLTSIHNEYFIVSMVDGARKAIDEGYFREYKGKILREYYK
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLPVWGNYITGWSIGRWSMFSVLKQTEYGRTGIISTCNGQIHTPAFIPVATKASIKGV
CCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCEECCEEEEEECCHHHCCC
LPEQMKQLGAQGLLANAYHLYLRPGPDTIAAAGGLSRFMSWNGPTFTDSGGFQVMSLGRG
CHHHHHHHCCCCEEECEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
GKKVISMEVPDNTWESNIRKTTLIRIDDQGVWFRSHIDGSQHRFTPEESIRVQNKIGADI
CCEEEEEECCCCCHHHCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCHH
IFAFDQLTTLDDSRAVQEYALERTRLWAERCLNQHSKEDQALFAVIQGANYRDLREKACR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHH
DLGQMGFDGFGIGGALEKSRIREIITWCTRKLPEEKPRHLLGISEPNDLMNAIEAGIDTF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCH
DCVSPTRVARNTAFYTPSGRKNLRRKMYRNDFSPLVEGCACYACSNFTRAYIHHLFRAKE
HCCCHHHHHHCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MCAATLTSIHNEYFIVSMVDGARKAIDEGYFREYKGKILREYYK
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA