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The map label for this gene is ispG

Identifier: 28572734

GI number: 28572734

Start: 658576

End: 659718

Strand: Direct

Name: ispG

Synonym: TW586

Alternate gene names: 28572734

Gene position: 658576-659718 (Clockwise)

Preceding gene: 28572733

Following gene: 28572736

Centisome position: 71.13

GC content: 50.04

Gene sequence:

>1143_bases
GTGTCAATTGACCTTGGTTTGCCAAAAGTGCGTGAGGTCTTGTCACCCCGCAGAAAGACAAGGCGCATAAAACTGGGCAA
AGTGTTCGTTGGCGGGGACTCGCCAATTCTTGTCCAGTCCATGACAACAACCCAGACAACAAATATAGACGCAACTCTAC
AGCAAATTGCAGAACTAACGGCTGCGGGTTGTGACATCGTTCGTGTTGCTGTCCCGAGTCGTGATGACGCCCAGGTGCTC
CATATAATAGCCAAGAAAAGCCAGATTCCCGTAATAGCCGATATACATTTCCAGCCAAACTATGTTTACGCAGCAATTGA
TGCCGGTTGCGCGGCAGTGCGTGTAAATCCAGGGAATATACGCAAGTTTGATGACCAGATTGGAAAGATCGCGGAAAAGG
CAAAAGCCGCAGGGGTGAGTATAAGAATTGGAGTCAATGCAGGTTCACTTGACCCACGACTTCTGGAGAAATACGGCCGA
CCAACCGCTCAGGCTCTTGTAGACAGTGCGGTGTGGGAAGCGGGTTTATTTGAGGAATATGATTTCCACGATTTTAAAAT
CTCCGTGAAACACCATGATCCCGTGACCATGGTAAAAGCCTATCGATTGTTAGCAGAAAGGGGTGATTGGCCCTTGCATC
TCGGCGTTACAGAGGCGGGGCCCGCCTTTCAGGGAACAATAAAATCTTCAACCGCTTTTGCTGTACTACTCGCAGAGGGA
ATAGGAGATACAATCCGTGTGTCACTATCCGCTCCGCCCGTCGAGGAAGTAAAAGTTGGTCTTGAAATACTGCGATCTCT
CGGCTTGCGTGAGAGACGACTGGAAATCGTATCATGTCCAAGTTGCGGTCGTGCGCAGGTTGATGTCTACCGCCTTGCCG
AGGAGGTAACAAAAGGCCTAGAGAAACTGACCGTTCCCCTCAGAGTTGCGGTTATGGGTTGTATTGTAAACGGCCCGGGT
GAGGCGCGAGAGGCCGATCTTGGAGTTGCAAGTGGAAATGGAAAGGGTCAGATTTTTGTTCGCGGACAGGTTATAAAAAC
CGTGCCAGAGTCCGAGATTGTCCCAACCCTGTTAGAAGAGGCGAATCGTCTCGCGGGGGAAATGCCATTCTCAAGTGGCA
GCCCAACAATTGCAATTGTATAA

Upstream 100 bases:

>100_bases
TGCCTGTTAGCTAGTATTAGTGATCTAACTAGTATTAGTGATCGCTTAAATATCGCTGCTCGTTCACAACAGCAAGACAA
GGCGCTGGTATCCTGTATGT

Downstream 100 bases:

>100_bases
ACGCAATCGCATAAAGCCAAGGTGAGTCAAGAGGCGAGTCAAGCGGATGTTACTTAGCGAACAGCGGCGCAGAAAACGCC
CTAAAGCCAACCTTTTACGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSIDLGLPKVREVLSPRRKTRRIKLGKVFVGGDSPILVQSMTTTQTTNIDATLQQIAELTAAGCDIVRVAVPSRDDAQVL
HIIAKKSQIPVIADIHFQPNYVYAAIDAGCAAVRVNPGNIRKFDDQIGKIAEKAKAAGVSIRIGVNAGSLDPRLLEKYGR
PTAQALVDSAVWEAGLFEEYDFHDFKISVKHHDPVTMVKAYRLLAERGDWPLHLGVTEAGPAFQGTIKSSTAFAVLLAEG
IGDTIRVSLSAPPVEEVKVGLEILRSLGLRERRLEIVSCPSCGRAQVDVYRLAEEVTKGLEKLTVPLRVAVMGCIVNGPG
EAREADLGVASGNGKGQIFVRGQVIKTVPESEIVPTLLEEANRLAGEMPFSSGSPTIAIV

Sequences:

>Translated_380_residues
MSIDLGLPKVREVLSPRRKTRRIKLGKVFVGGDSPILVQSMTTTQTTNIDATLQQIAELTAAGCDIVRVAVPSRDDAQVL
HIIAKKSQIPVIADIHFQPNYVYAAIDAGCAAVRVNPGNIRKFDDQIGKIAEKAKAAGVSIRIGVNAGSLDPRLLEKYGR
PTAQALVDSAVWEAGLFEEYDFHDFKISVKHHDPVTMVKAYRLLAERGDWPLHLGVTEAGPAFQGTIKSSTAFAVLLAEG
IGDTIRVSLSAPPVEEVKVGLEILRSLGLRERRLEIVSCPSCGRAQVDVYRLAEEVTKGLEKLTVPLRVAVMGCIVNGPG
EAREADLGVASGNGKGQIFVRGQVIKTVPESEIVPTLLEEANRLAGEMPFSSGSPTIAIV
>Mature_379_residues
SIDLGLPKVREVLSPRRKTRRIKLGKVFVGGDSPILVQSMTTTQTTNIDATLQQIAELTAAGCDIVRVAVPSRDDAQVLH
IIAKKSQIPVIADIHFQPNYVYAAIDAGCAAVRVNPGNIRKFDDQIGKIAEKAKAAGVSIRIGVNAGSLDPRLLEKYGRP
TAQALVDSAVWEAGLFEEYDFHDFKISVKHHDPVTMVKAYRLLAERGDWPLHLGVTEAGPAFQGTIKSSTAFAVLLAEGI
GDTIRVSLSAPPVEEVKVGLEILRSLGLRERRLEIVSCPSCGRAQVDVYRLAEEVTKGLEKLTVPLRVAVMGCIVNGPGE
AREADLGVASGNGKGQIFVRGQVIKTVPESEIVPTLLEEANRLAGEMPFSSGSPTIAIV

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=326, Percent_Identity=46.0122699386503, Blast_Score=300, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_TROW8 (Q83NE4)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789514.1
- ProteinModelPortal:   Q83NE4
- STRING:   Q83NE4
- GeneID:   1064790
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW586
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   INEDTMD
- PhylomeDB:   Q83NE4
- ProtClustDB:   PRK00366
- BioCyc:   TWHI218496:TW0559-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40810; Mature: 40678

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDLGLPKVREVLSPRRKTRRIKLGKVFVGGDSPILVQSMTTTQTTNIDATLQQIAELT
CCCCCCCHHHHHHHCCHHHHCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AAGCDIVRVAVPSRDDAQVLHIIAKKSQIPVIADIHFQPNYVYAAIDAGCAAVRVNPGNI
HCCCCEEEEECCCCCCHHHHHEEHHCCCCCEEEEEEECCCEEEEEECCCEEEEEECCCCC
RKFDDQIGKIAEKAKAAGVSIRIGVNAGSLDPRLLEKYGRPTAQALVDSAVWEAGLFEEY
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
DFHDFKISVKHHDPVTMVKAYRLLAERGDWPLHLGVTEAGPAFQGTIKSSTAFAVLLAEG
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECC
IGDTIRVSLSAPPVEEVKVGLEILRSLGLRERRLEIVSCPSCGRAQVDVYRLAEEVTKGL
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
EKLTVPLRVAVMGCIVNGPGEAREADLGVASGNGKGQIFVRGQVIKTVPESEIVPTLLEE
HHHHHHHEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEEEECCCCCHHHHHHHHH
ANRLAGEMPFSSGSPTIAIV
HHHHHCCCCCCCCCCEEEEC
>Mature Secondary Structure 
SIDLGLPKVREVLSPRRKTRRIKLGKVFVGGDSPILVQSMTTTQTTNIDATLQQIAELT
CCCCCCHHHHHHHCCHHHHCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AAGCDIVRVAVPSRDDAQVLHIIAKKSQIPVIADIHFQPNYVYAAIDAGCAAVRVNPGNI
HCCCCEEEEECCCCCCHHHHHEEHHCCCCCEEEEEEECCCEEEEEECCCEEEEEECCCCC
RKFDDQIGKIAEKAKAAGVSIRIGVNAGSLDPRLLEKYGRPTAQALVDSAVWEAGLFEEY
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
DFHDFKISVKHHDPVTMVKAYRLLAERGDWPLHLGVTEAGPAFQGTIKSSTAFAVLLAEG
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECC
IGDTIRVSLSAPPVEEVKVGLEILRSLGLRERRLEIVSCPSCGRAQVDVYRLAEEVTKGL
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
EKLTVPLRVAVMGCIVNGPGEAREADLGVASGNGKGQIFVRGQVIKTVPESEIVPTLLEE
HHHHHHHEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEEEECCCCCHHHHHHHHH
ANRLAGEMPFSSGSPTIAIV
HHHHHCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174