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The map label for this gene is proS [H]

Identifier: 28572736

GI number: 28572736

Start: 660811

End: 662616

Strand: Direct

Name: proS [H]

Synonym: TW588

Alternate gene names: 28572736

Gene position: 660811-662616 (Clockwise)

Preceding gene: 28572734

Following gene: 28572745

Centisome position: 71.37

GC content: 45.96

Gene sequence:

>1806_bases
ATGATTACAAAGGTCTCCGATTTTCTTTTCAGGACATTTCGTGAAGATCCGGCAACCACAGAAAGCAGGGGTTACGGCTT
TCTTCTTCGGGCAGGATACATAAGACAAACAGGGTCTGGAATATTTTCTTGGATGCCACTCGGACTAAAAGTGCGACACA
AAATTGAAAATATTATCCGCTATGAAATGAGACAGGTTAATGCAATCGAAGTGTTGTTCCCCGCGCTTTTCTCGGCAGAT
TTATTCAAACAAAGTGGGCGCTGGAGCGAATATGGCGATGATATATTCAGGCTAAAAGATCGACGGCAGGGTGACTATTT
GCTTGCTCCAACTCATGAAGAAGCATTTACTCAGATGATGAAGGAAATTTGCACATCATATAGAGATTTACCAAGAACTG
TCTATCAGATCCAAGATAAGTACAGAGATGAGCTAAGGCCCAGGGCTGGACTGCTGCGCAGTCGGGAGTTCTCGATGAAA
GATGCGTACAGTTTCGACCTCGACGAAAAAGGGCTACGACAGAGCTATGAGGCTCAAAAGAGGGCTTATAAAAAAATTTT
TGATCGCCTGGAAATTGATTATGTCATAGTCAAGGCTAATGCCGGGGCGATGGGCGGATCTGTGAGCGAAGAATTCCTTC
ACCCAACTGAAATGGGCGATGACACTTTTGTTGTTACCGCTGACGGGTCTGCTTTTAATGCAGAGGTTTACGTTACGCTA
CCCGGTCCGGCAATTGATTATTCCAATGCGCCGGAGGCAGAGGATTGTGAAACCCCCGGGGTTATCAGTATTCCAGATCT
TGTGAATCACATGAATTCTTCCGGACGGTTTATTGGGCGGGTGATTGAATCTTCGGATTGCCTAAAATGTCTGTTATTCA
GGATAGAATACGCCGAGGTGCAGAATGGCAATCCAGGTAATTTGGTAGTAAAAAAAATACTCGAGAGGGGTTTTGAATAT
ATTGGCTTTTTAGTGCCGGGCGATAGAAATGTGGATCTAAAAAGGGCACAGGTGGCACTCTCACCGCTCACGATTGAACC
GGCTGATAATAGAGTTTTCGAATGCAATCCATCCTTTGTAAGGGGAAGCATTGGCCCGGGCCTTAGTGGTGTATTTTATT
GTGCAGACCCAAGAGTATCCTCGGGAAGCAGTTGGATAATCGGGGCAAATAGACCAGGTGTGCACCGTATCGGTGCCATT
GCTGGAAGAGATTTTTCATTTGATTGCACCCTTGATGTATCCTCGATAAAAACCGGTGATAAAAGCGAGTGGGGTCCCGT
TACCGTAAAAAGGGGTATTGAAATCGGTCATTTATTTCAATTGGGACTGAAATATTCAAATGCCCTCGGATTAAAGGTGC
TAGACAAAGATGGTTATAACAAAGCCGTTTTTATGGGATCCTACGGTATAGGTGTCAGTAGGCTGTTTGCTCTTATTGCC
GAAAAAAATTGCGATGAGCGTGGTTTGAAATGGCCTGCGGTACTTGCCCCATTCGATCTGCATGTAGTTTTACTATCTTC
GGCACGTGCTGAGCTGATCGATAGCCTGACAGACTGCGGGCTGGATGTCCTGGTTGATGACAGACGCGTCAGTCCCGGTG
TTAAATTCACCGATGCTCAGTTGATTGGTGTGCCAAAAATAATTGTTATCGGTGATAAGACCCGAGGGGAAGATGTGGAA
GTGTGGGATAGGGCTAATGACCAGCGGACAGTGCTCCCCCTCAAAGAGATGATACAGGGGGTGATACAGAGAAGTGATAC
AGGGGGGTGTACAGAGAGGTGTACAGGTGTATGCCCCACGAGATAA

Upstream 100 bases:

>100_bases
CCAATAGATATTGGGTTTATATATAGAGTTTATAGATACTGGGTTTATAAGCGTCATGGCTGCACATAATGTGAACTGAA
GAACACGATATACTGCACCC

Downstream 100 bases:

>100_bases
ATACCGAGAGGTAAATTCCACAGCAGAATAACAGGAATGACACCCAGGCATGACACCGGCCCTGACGCGTCTACAATCCT
GTGTTCGCCCGGATGGTGTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MITKVSDFLFRTFREDPATTESRGYGFLLRAGYIRQTGSGIFSWMPLGLKVRHKIENIIRYEMRQVNAIEVLFPALFSAD
LFKQSGRWSEYGDDIFRLKDRRQGDYLLAPTHEEAFTQMMKEICTSYRDLPRTVYQIQDKYRDELRPRAGLLRSREFSMK
DAYSFDLDEKGLRQSYEAQKRAYKKIFDRLEIDYVIVKANAGAMGGSVSEEFLHPTEMGDDTFVVTADGSAFNAEVYVTL
PGPAIDYSNAPEAEDCETPGVISIPDLVNHMNSSGRFIGRVIESSDCLKCLLFRIEYAEVQNGNPGNLVVKKILERGFEY
IGFLVPGDRNVDLKRAQVALSPLTIEPADNRVFECNPSFVRGSIGPGLSGVFYCADPRVSSGSSWIIGANRPGVHRIGAI
AGRDFSFDCTLDVSSIKTGDKSEWGPVTVKRGIEIGHLFQLGLKYSNALGLKVLDKDGYNKAVFMGSYGIGVSRLFALIA
EKNCDERGLKWPAVLAPFDLHVVLLSSARAELIDSLTDCGLDVLVDDRRVSPGVKFTDAQLIGVPKIIVIGDKTRGEDVE
VWDRANDQRTVLPLKEMIQGVIQRSDTGGCTERCTGVCPTR

Sequences:

>Translated_601_residues
MITKVSDFLFRTFREDPATTESRGYGFLLRAGYIRQTGSGIFSWMPLGLKVRHKIENIIRYEMRQVNAIEVLFPALFSAD
LFKQSGRWSEYGDDIFRLKDRRQGDYLLAPTHEEAFTQMMKEICTSYRDLPRTVYQIQDKYRDELRPRAGLLRSREFSMK
DAYSFDLDEKGLRQSYEAQKRAYKKIFDRLEIDYVIVKANAGAMGGSVSEEFLHPTEMGDDTFVVTADGSAFNAEVYVTL
PGPAIDYSNAPEAEDCETPGVISIPDLVNHMNSSGRFIGRVIESSDCLKCLLFRIEYAEVQNGNPGNLVVKKILERGFEY
IGFLVPGDRNVDLKRAQVALSPLTIEPADNRVFECNPSFVRGSIGPGLSGVFYCADPRVSSGSSWIIGANRPGVHRIGAI
AGRDFSFDCTLDVSSIKTGDKSEWGPVTVKRGIEIGHLFQLGLKYSNALGLKVLDKDGYNKAVFMGSYGIGVSRLFALIA
EKNCDERGLKWPAVLAPFDLHVVLLSSARAELIDSLTDCGLDVLVDDRRVSPGVKFTDAQLIGVPKIIVIGDKTRGEDVE
VWDRANDQRTVLPLKEMIQGVIQRSDTGGCTERCTGVCPTR
>Mature_601_residues
MITKVSDFLFRTFREDPATTESRGYGFLLRAGYIRQTGSGIFSWMPLGLKVRHKIENIIRYEMRQVNAIEVLFPALFSAD
LFKQSGRWSEYGDDIFRLKDRRQGDYLLAPTHEEAFTQMMKEICTSYRDLPRTVYQIQDKYRDELRPRAGLLRSREFSMK
DAYSFDLDEKGLRQSYEAQKRAYKKIFDRLEIDYVIVKANAGAMGGSVSEEFLHPTEMGDDTFVVTADGSAFNAEVYVTL
PGPAIDYSNAPEAEDCETPGVISIPDLVNHMNSSGRFIGRVIESSDCLKCLLFRIEYAEVQNGNPGNLVVKKILERGFEY
IGFLVPGDRNVDLKRAQVALSPLTIEPADNRVFECNPSFVRGSIGPGLSGVFYCADPRVSSGSSWIIGANRPGVHRIGAI
AGRDFSFDCTLDVSSIKTGDKSEWGPVTVKRGIEIGHLFQLGLKYSNALGLKVLDKDGYNKAVFMGSYGIGVSRLFALIA
EKNCDERGLKWPAVLAPFDLHVVLLSSARAELIDSLTDCGLDVLVDDRRVSPGVKFTDAQLIGVPKIIVIGDKTRGEDVE
VWDRANDQRTVLPLKEMIQGVIQRSDTGGCTERCTGVCPTR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=211, Percent_Identity=36.9668246445498, Blast_Score=157, Evalue=4e-38,
Organism=Escherichia coli, GI1786392, Length=600, Percent_Identity=36.5, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788013, Length=185, Percent_Identity=23.2432432432432, Blast_Score=63, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI115532348, Length=206, Percent_Identity=33.495145631068, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71984184, Length=182, Percent_Identity=24.7252747252747, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=182, Percent_Identity=24.7252747252747, Blast_Score=70, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=552, Percent_Identity=30.6159420289855, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24656200, Length=240, Percent_Identity=34.5833333333333, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 67006; Mature: 67006

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKVSDFLFRTFREDPATTESRGYGFLLRAGYIRQTGSGIFSWMPLGLKVRHKIENIIR
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHCCCCCEEECCCCHHHHHHHHHHHH
YEMRQVNAIEVLFPALFSADLFKQSGRWSEYGDDIFRLKDRRQGDYLLAPTHEEAFTQMM
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCEEEECCCHHHHHHHH
KEICTSYRDLPRTVYQIQDKYRDELRPRAGLLRSREFSMKDAYSFDLDEKGLRQSYEAQK
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
RAYKKIFDRLEIDYVIVKANAGAMGGSVSEEFLHPTEMGDDTFVVTADGSAFNAEVYVTL
HHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEC
PGPAIDYSNAPEAEDCETPGVISIPDLVNHMNSSGRFIGRVIESSDCLKCLLFRIEYAEV
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHC
QNGNPGNLVVKKILERGFEYIGFLVPGDRNVDLKRAQVALSPLTIEPADNRVFECNPSFV
CCCCCHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHEEEECCEEECCCCCCEEECCCCCE
RGSIGPGLSGVFYCADPRVSSGSSWIIGANRPGVHRIGAIAGRDFSFDCTLDVSSIKTGD
ECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHEECCCCEEEEEEEHHHCCCCC
KSEWGPVTVKRGIEIGHLFQLGLKYSNALGLKVLDKDGYNKAVFMGSYGIGVSRLFALIA
CCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHH
EKNCDERGLKWPAVLAPFDLHVVLLSSARAELIDSLTDCGLDVLVDDRRVSPGVKFTDAQ
HCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCCE
LIGVPKIIVIGDKTRGEDVEVWDRANDQRTVLPLKEMIQGVIQRSDTGGCTERCTGVCPT
EECCCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
R
C
>Mature Secondary Structure
MITKVSDFLFRTFREDPATTESRGYGFLLRAGYIRQTGSGIFSWMPLGLKVRHKIENIIR
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHCCCCCEEECCCCHHHHHHHHHHHH
YEMRQVNAIEVLFPALFSADLFKQSGRWSEYGDDIFRLKDRRQGDYLLAPTHEEAFTQMM
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCEEEECCCHHHHHHHH
KEICTSYRDLPRTVYQIQDKYRDELRPRAGLLRSREFSMKDAYSFDLDEKGLRQSYEAQK
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
RAYKKIFDRLEIDYVIVKANAGAMGGSVSEEFLHPTEMGDDTFVVTADGSAFNAEVYVTL
HHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEC
PGPAIDYSNAPEAEDCETPGVISIPDLVNHMNSSGRFIGRVIESSDCLKCLLFRIEYAEV
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHC
QNGNPGNLVVKKILERGFEYIGFLVPGDRNVDLKRAQVALSPLTIEPADNRVFECNPSFV
CCCCCHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHEEEECCEEECCCCCCEEECCCCCE
RGSIGPGLSGVFYCADPRVSSGSSWIIGANRPGVHRIGAIAGRDFSFDCTLDVSSIKTGD
ECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHEECCCCEEEEEEEHHHCCCCC
KSEWGPVTVKRGIEIGHLFQLGLKYSNALGLKVLDKDGYNKAVFMGSYGIGVSRLFALIA
CCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHH
EKNCDERGLKWPAVLAPFDLHVVLLSSARAELIDSLTDCGLDVLVDDRRVSPGVKFTDAQ
HCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCCE
LIGVPKIIVIGDKTRGEDVEVWDRANDQRTVLPLKEMIQGVIQRSDTGGCTERCTGVCPT
EECCCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]