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The map label for this gene is smpB

Identifier: 28572719

GI number: 28572719

Start: 623081

End: 623716

Strand: Reverse

Name: smpB

Synonym: TW571

Alternate gene names: 28572719

Gene position: 623716-623081 (Counterclockwise)

Preceding gene: 28572720

Following gene: 28572718

Centisome position: 67.36

GC content: 45.13

Gene sequence:

>636_bases
GTGCACAGGCGATCTTCTAAGAGTTACTCTTCCAGGAATTCTAAAACTCCAGAGCGTTCTAAAATTCCAGATTGCGTTAT
CGCATCAAACAAAAGCGCAGAATATAACTATTTTCTGGACAAAACATTTGAGGCAGGCCTTGTCCTACTGGGCAGTGAGG
TAAAAAGCGTCAGACAGCAAAAAATAAGTCTGTCTGAGGCCTATATTTATGAGGATAACTCAGAAATATGGCTTGCCAAT
CTGTATATCCCAGAGCACAAAACGGGGGGATGGACCAATCATAACCCCCGGCGACTCAGAAAACTATTGTTACACAAGTA
CCAAATTGCACGTCTACGAAAAGATCTATTAATCCCTGGTATAACCCTGGTGCCCATCAAGTTTTATTTCAGGAACGGCC
GCGCGAAGCTTCTTATATCCCTTGCGCGAGGTAAAAAGCTAATTGACAAGCGCGAGGTAATAAAAGAGCGAGAGGCCACT
CTAGAAGCAAACCGTGCCCTAAAACATCGCTTACGCAGACCCCGTGCACAGCGCAATACACAACGCAGTGTAACACCGCG
CAGCACGCGGGAAAATAAAAATGTACAGAGAAATAAGGCAAGGTCTGCCAGGTGCAATGTACGGCATGAAAACTAA

Upstream 100 bases:

>100_bases
TGATAATCGTTCCGATTATTTTTGGGATTGCCATTCTCCTTTCGGGATTATCTGCAAAATTCTCGATTTCTCGTTCACTT
CGGGTCTAAGGGTTCAAGCT

Downstream 100 bases:

>100_bases
TAAGCGACAACTAATAATAGGTGCCTTAGCAACCATTCTGACATCTATCCTCACACTCTTTTTTGGTCACCGAGTTATTT
AAACTGATTATTTTGTATTT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MHRRSSKSYSSRNSKTPERSKIPDCVIASNKSAEYNYFLDKTFEAGLVLLGSEVKSVRQQKISLSEAYIYEDNSEIWLAN
LYIPEHKTGGWTNHNPRRLRKLLLHKYQIARLRKDLLIPGITLVPIKFYFRNGRAKLLISLARGKKLIDKREVIKEREAT
LEANRALKHRLRRPRAQRNTQRSVTPRSTRENKNVQRNKARSARCNVRHEN

Sequences:

>Translated_211_residues
MHRRSSKSYSSRNSKTPERSKIPDCVIASNKSAEYNYFLDKTFEAGLVLLGSEVKSVRQQKISLSEAYIYEDNSEIWLAN
LYIPEHKTGGWTNHNPRRLRKLLLHKYQIARLRKDLLIPGITLVPIKFYFRNGRAKLLISLARGKKLIDKREVIKEREAT
LEANRALKHRLRRPRAQRNTQRSVTPRSTRENKNVQRNKARSARCNVRHEN
>Mature_211_residues
MHRRSSKSYSSRNSKTPERSKIPDCVIASNKSAEYNYFLDKTFEAGLVLLGSEVKSVRQQKISLSEAYIYEDNSEIWLAN
LYIPEHKTGGWTNHNPRRLRKLLLHKYQIARLRKDLLIPGITLVPIKFYFRNGRAKLLISLARGKKLIDKREVIKEREAT
LEANRALKHRLRRPRAQRNTQRSVTPRSTRENKNVQRNKARSARCNVRHEN

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=37.4149659863946, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_TROW8 (Q83NF6)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789499.1
- ProteinModelPortal:   Q83NF6
- SMR:   Q83NF6
- STRING:   Q83NF6
- GeneID:   1064775
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW571
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   TWHI218496:TW0544-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 24747; Mature: 24747

Theoretical pI: Translated: 11.43; Mature: 11.43

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRSSKSYSSRNSKTPERSKIPDCVIASNKSAEYNYFLDKTFEAGLVLLGSEVKSVRQQ
CCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCCEEEEECCCCCCCEEEECHHHHHHHHH
KISLSEAYIYEDNSEIWLANLYIPEHKTGGWTNHNPRRLRKLLLHKYQIARLRKDLLIPG
HHCHHHEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITLVPIKFYFRNGRAKLLISLARGKKLIDKREVIKEREATLEANRALKHRLRRPRAQRNT
CCEEEEEEEEECCCHHEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
QRSVTPRSTRENKNVQRNKARSARCNVRHEN
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHRRSSKSYSSRNSKTPERSKIPDCVIASNKSAEYNYFLDKTFEAGLVLLGSEVKSVRQQ
CCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCCEEEEECCCCCCCEEEECHHHHHHHHH
KISLSEAYIYEDNSEIWLANLYIPEHKTGGWTNHNPRRLRKLLLHKYQIARLRKDLLIPG
HHCHHHEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITLVPIKFYFRNGRAKLLISLARGKKLIDKREVIKEREATLEANRALKHRLRRPRAQRNT
CCEEEEEEEEECCCHHEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
QRSVTPRSTRENKNVQRNKARSARCNVRHEN
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174