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The map label for this gene is murD

Identifier: 28572692

GI number: 28572692

Start: 579308

End: 580750

Strand: Reverse

Name: murD

Synonym: TW544

Alternate gene names: 28572692

Gene position: 580750-579308 (Counterclockwise)

Preceding gene: 28572693

Following gene: 28572691

Centisome position: 62.72

GC content: 45.18

Gene sequence:

>1443_bases
ATGTCGAGGGTTGAGGGGCTTACAAGTTGGTATTCAGATTGGCAAGGTCTTTCTGCGGCAATTCTGGGGATTGGGGTTTC
AGGTTTTGCCGCCGCCGATTCATTACGTGAACTGGGTGTTGATGTTACCGTATATGCCCCGGAAAAGCATACGCGGTATA
ACAAGCTTCTTGATGCGATTGGTGCAAGATATGTGTGTGCATATCTTGACGAATTGTGCGAGGTAGATGTTGATTTTATC
GTGGTTTCGCCTGGTATTTCTCCTGATAATCCCGTGATTAAACGATTACGTGACAGGCAGATCCCCATATTGAGTGAGAT
TGAGCTTGCATGGCGCGTCAGGGACAAAGTCAATACTTGTCCATGGATATTGATTACAGGCACGAATGGCAAAACAACAA
CTGCTCTACTTACGGGCAGTATGCTTGCTAAAGACGGTGCGCGGGTTGCTGTCTGCGGAAATATTGGCACTCCAGTTCTT
GATGCCGTAAGAAACCCCAAGGGGTTTGATTATTTTGTTGTTGAGCTGTCCAGTTTTCAGCTCTCACTGCTCCCGATGCA
CGGAAATGGTGCGGTTAAGGGTTTTTCTTCTGCTTGTGTGAATCTTGATGAAGACCACCTTGAATGGCACGGCGCAAAGG
AATTGTATTACCGTGCAAAGTCTCGTGTTTACCACGGCACAACAGGTTTTTGTGTGTACAACCTAGATGATGAAGAGACA
AAGAAAATGGTTGAACAAGCATGTGTTGCGCGAAATGTTAGGGCAATTGGTTTCGGTCTTTGCGTGCCAGATGTGGGCCA
GGTTGGAATCGTTGATGGAATTCTGTGCGATAGGGCATTTTTATCCGCGAGAAAAGACAGTGCACTTGAGATTACAAGTG
TGGAAAAACTTGAAAAAAACAAATTATCGATGCGGCATATTATTTCCGATGTGCTTTGTGCTGTAGCTCTGGCTAGGTCG
GTTGAAACAAACCCGTTGTCGATATCCCGGGCATTGGATGAGTTTTGCCTATCACCTCATAGGACCGAAGTGGTCGCAAA
AGAGATGGGGGTTATGTGGGTAAATGACTCAAAGGCAACCAATCCACACGCAGTTATTGCATCTCTCTCGAATTTTTCCA
GAGTAATTTTGATTTTTGGCGGCCTTATGAAGGGTGTTGATGTTTCTGGTATATTCGACAGGTTTTATGAAACAATAAAG
GCTGTTGTAGTAATCGGCAAAAATCAATCCTTTGTGGGGAACATAAAGTGCAAGAAGATTGTATGCATACCCGATTCAAA
TGATCCGATGTCAGAAGCCGTCGCCGCCGCAGATCTGCTCGCCACTCCTGGTGATACCGTACTACTGTCACCGGGTGGTT
CATCATTTGATCAGTTTGAGAGCTATGAGCATCGAGGCAATTGTTTTATAAATGCCGTGAAAGATCTTGTCAAGCGTAAA
TAA

Upstream 100 bases:

>100_bases
GGCGATGATCCTTATCTTGATGTCCTCGGGAAGAAAATTCCGTATTCTCTCAGAGATGAAGTGCGCAGGGGACTTGAAAG
ATTTGCGCAGGGGACTTGAA

Downstream 100 bases:

>100_bases
TGACAAAGCATAATAGGCTCATATCAGCAAATTATTTTTTATTATTGGCCAATGTGTTCGCACTGAATGCAATTGGGCTG
GTTATGGTTATATCGGCATC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRVEGLTSWYSDWQGLSAAILGIGVSGFAAADSLRELGVDVTVYAPEKHTRYNKLLDAIGARYVCAYLDELCEVDVDFI
VVSPGISPDNPVIKRLRDRQIPILSEIELAWRVRDKVNTCPWILITGTNGKTTTALLTGSMLAKDGARVAVCGNIGTPVL
DAVRNPKGFDYFVVELSSFQLSLLPMHGNGAVKGFSSACVNLDEDHLEWHGAKELYYRAKSRVYHGTTGFCVYNLDDEET
KKMVEQACVARNVRAIGFGLCVPDVGQVGIVDGILCDRAFLSARKDSALEITSVEKLEKNKLSMRHIISDVLCAVALARS
VETNPLSISRALDEFCLSPHRTEVVAKEMGVMWVNDSKATNPHAVIASLSNFSRVILIFGGLMKGVDVSGIFDRFYETIK
AVVVIGKNQSFVGNIKCKKIVCIPDSNDPMSEAVAAADLLATPGDTVLLSPGGSSFDQFESYEHRGNCFINAVKDLVKRK

Sequences:

>Translated_480_residues
MSRVEGLTSWYSDWQGLSAAILGIGVSGFAAADSLRELGVDVTVYAPEKHTRYNKLLDAIGARYVCAYLDELCEVDVDFI
VVSPGISPDNPVIKRLRDRQIPILSEIELAWRVRDKVNTCPWILITGTNGKTTTALLTGSMLAKDGARVAVCGNIGTPVL
DAVRNPKGFDYFVVELSSFQLSLLPMHGNGAVKGFSSACVNLDEDHLEWHGAKELYYRAKSRVYHGTTGFCVYNLDDEET
KKMVEQACVARNVRAIGFGLCVPDVGQVGIVDGILCDRAFLSARKDSALEITSVEKLEKNKLSMRHIISDVLCAVALARS
VETNPLSISRALDEFCLSPHRTEVVAKEMGVMWVNDSKATNPHAVIASLSNFSRVILIFGGLMKGVDVSGIFDRFYETIK
AVVVIGKNQSFVGNIKCKKIVCIPDSNDPMSEAVAAADLLATPGDTVLLSPGGSSFDQFESYEHRGNCFINAVKDLVKRK
>Mature_479_residues
SRVEGLTSWYSDWQGLSAAILGIGVSGFAAADSLRELGVDVTVYAPEKHTRYNKLLDAIGARYVCAYLDELCEVDVDFIV
VSPGISPDNPVIKRLRDRQIPILSEIELAWRVRDKVNTCPWILITGTNGKTTTALLTGSMLAKDGARVAVCGNIGTPVLD
AVRNPKGFDYFVVELSSFQLSLLPMHGNGAVKGFSSACVNLDEDHLEWHGAKELYYRAKSRVYHGTTGFCVYNLDDEETK
KMVEQACVARNVRAIGFGLCVPDVGQVGIVDGILCDRAFLSARKDSALEITSVEKLEKNKLSMRHIISDVLCAVALARSV
ETNPLSISRALDEFCLSPHRTEVVAKEMGVMWVNDSKATNPHAVIASLSNFSRVILIFGGLMKGVDVSGIFDRFYETIKA
VVVIGKNQSFVGNIKCKKIVCIPDSNDPMSEAVAAADLLATPGDTVLLSPGGSSFDQFESYEHRGNCFINAVKDLVKRK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=474, Percent_Identity=30.168776371308, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_TROW8 (Q83HK0)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789472.1
- ProteinModelPortal:   Q83HK0
- STRING:   Q83HK0
- GeneID:   1064747
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW544
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- PhylomeDB:   Q83HK0
- ProtClustDB:   PRK01438
- BioCyc:   TWHI218496:TW0517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 52324; Mature: 52192

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVEGLTSWYSDWQGLSAAILGIGVSGFAAADSLRELGVDVTVYAPEKHTRYNKLLDAI
CCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
GARYVCAYLDELCEVDVDFIVVSPGISPDNPVIKRLRDRQIPILSEIELAWRVRDKVNTC
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
PWILITGTNGKTTTALLTGSMLAKDGARVAVCGNIGTPVLDAVRNPKGFDYFVVELSSFQ
CEEEEECCCCCEEEEEEECHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEEEECCE
LSLLPMHGNGAVKGFSSACVNLDEDHLEWHGAKELYYRAKSRVYHGTTGFCVYNLDDEET
EEEEEECCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHHHCCEEECCCEEEEEECCCHHH
KKMVEQACVARNVRAIGFGLCVPDVGQVGIVDGILCDRAFLSARKDSALEITSVEKLEKN
HHHHHHHHHHCCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
KLSMRHIISDVLCAVALARSVETNPLSISRALDEFCLSPHRTEVVAKEMGVMWVNDSKAT
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCC
NPHAVIASLSNFSRVILIFGGLMKGVDVSGIFDRFYETIKAVVVIGKNQSFVGNIKCKKI
CCHHHHEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCCCEEECEEEEEE
VCIPDSNDPMSEAVAAADLLATPGDTVLLSPGGSSFDQFESYEHRGNCFINAVKDLVKRK
EEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SRVEGLTSWYSDWQGLSAAILGIGVSGFAAADSLRELGVDVTVYAPEKHTRYNKLLDAI
CCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
GARYVCAYLDELCEVDVDFIVVSPGISPDNPVIKRLRDRQIPILSEIELAWRVRDKVNTC
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
PWILITGTNGKTTTALLTGSMLAKDGARVAVCGNIGTPVLDAVRNPKGFDYFVVELSSFQ
CEEEEECCCCCEEEEEEECHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEEEECCE
LSLLPMHGNGAVKGFSSACVNLDEDHLEWHGAKELYYRAKSRVYHGTTGFCVYNLDDEET
EEEEEECCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHHHCCEEECCCEEEEEECCCHHH
KKMVEQACVARNVRAIGFGLCVPDVGQVGIVDGILCDRAFLSARKDSALEITSVEKLEKN
HHHHHHHHHHCCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
KLSMRHIISDVLCAVALARSVETNPLSISRALDEFCLSPHRTEVVAKEMGVMWVNDSKAT
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCC
NPHAVIASLSNFSRVILIFGGLMKGVDVSGIFDRFYETIKAVVVIGKNQSFVGNIKCKKI
CCHHHHEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCCCEEECEEEEEE
VCIPDSNDPMSEAVAAADLLATPGDTVLLSPGGSSFDQFESYEHRGNCFINAVKDLVKRK
EEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174