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The map label for this gene is murE

Identifier: 28572693

GI number: 28572693

Start: 580752

End: 582281

Strand: Reverse

Name: murE

Synonym: TW545

Alternate gene names: 28572693

Gene position: 582281-580752 (Counterclockwise)

Preceding gene: 28572694

Following gene: 28572692

Centisome position: 62.89

GC content: 44.38

Gene sequence:

>1530_bases
GTGACTTTGCAGGGTAAACCATACAGATTATCTGATCTAATAAAACAAACAGGTGTGCGCCTTACCGATTGTTCGAATCA
GTTCTCAGATAGGTTTGTATCCGGGATAACACAAATCGCTCAGAGTGTTGAACGGGATGATATATTCGTTGCGTTTCAGG
GTAAAACTCGTCACGGAGTTGAATTTCTAGATCAGGTACAAAGCTGTGCCGCGGTGCTGACTGACAATAAAGGCAGGCAT
ATCATGCAGTCTGATTGTTTACCAACACGCAGTACGCCGATTCTTGTTACAGATTCTCCTCGTTCAGATCTTATCGTGCT
AGCAAAGAGAGTGTATCCGATCGATGATATTCGCATTTTCGGAATTACCGGGACAAATGGAAAAACAAGCACAATGCATA
TTGCTGCCAAGCTGCTTGAAATGATGGGCATTTCGTGTGGCATTAGCACGACAATAGGCAGTTCCGCTTCAGAGTCTGAC
AGTTGTCTAACAACCCCAGAACTATGCCAGCTATATGCTCGTATTTTCACCGCAAAACAGGCACGGGCGGACTTTTTTGC
CCTTGAAGCTTCCTCGCACGCAATAAATCGAGGCAGGCTCGGGGACATTGTTCTCGAAGTTGCTGCGTTCACCAATCTTA
CCCCAGAGCATATGGAAGAGCACAAAAATATGGAGGCTTATTATCAAGCAAAAAAGGCTTTGTTTTTGAATAAGAGGTCA
AACAGTGCGGTTATCAATATTGATACACCATACGGGATACGTCTTTTTAAAGAAACGGGCTGCAGTGCTTCGGTTATTTC
AGAAAACACAAAATACGGGTTGGACCACAAGCTATTCTGGCAGGCAAGCGTTCGTAGAGTTGGGTTGTCATTCGGATTTA
CGCTTATCTCTCCGAGCGGTTACCGCGTTGAAAGCAGTATTAGTTTGCTTGGTAAAGCATTTGCTTTAAATACATGTATG
GCAATAGTTATTCTTTGTAATCTTGGTATCGATATAGAAAGAATTGACAGTGTTCTACGTAAGGCCGGCGGTCTAAAAAT
GGTTTTACCAGGTCGAATGGAGGTTTTTCAGACAGGAAACAGTCCGCGAGTAATTGTTGATCATGGACATACTGTGGATG
CGGTTGAAACAGCGCTTGTTGCAGCCAAATCGATAACTCGAGGTCGGCTTATAACAATCATTAATGCCGATGGTCAGAGG
GATCCCTCAAAGCGTAAGCATCTTGGGCAGCTCTGCGGAGCTTACAGTGACAAATTGTTTATAACTGACGGGCATTCCCG
CTTTGAAAATCCAGCAGAAATAAGACGCATGATTCTTGACGGAGTTGAAGGTCCTCGAAAGCAAGTAGAGCAGATACCCT
CTATGACACAGGCTGTTTTGGCGGCAATTGATATTGCTAAATCCGATGACACCGTTTTATGCTCTGGTTTTGGCGATGAT
CCTTATCTTGATGTCCTCGGGAAGAAAATTCCGTATTCTCTCAGAGATGAAGTGCGCAGGGGACTTGAAAGATTTGCGCA
GGGGACTTGA

Upstream 100 bases:

>100_bases
AAGGTTGGGCTGAAATCACTGTTGTTGTGCGTTTTTGGATAATTGCAGGTTTATTCACTGCCCTTGGCATTGGTTTATTT
TATGCGGATTGGCTATATTC

Downstream 100 bases:

>100_bases
AATGTCGAGGGTTGAGGGGCTTACAAGTTGGTATTCAGATTGGCAAGGTCTTTCTGCGGCAATTCTGGGGATTGGGGTTT
CAGGTTTTGCCGCCGCCGAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diami nopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTLQGKPYRLSDLIKQTGVRLTDCSNQFSDRFVSGITQIAQSVERDDIFVAFQGKTRHGVEFLDQVQSCAAVLTDNKGRH
IMQSDCLPTRSTPILVTDSPRSDLIVLAKRVYPIDDIRIFGITGTNGKTSTMHIAAKLLEMMGISCGISTTIGSSASESD
SCLTTPELCQLYARIFTAKQARADFFALEASSHAINRGRLGDIVLEVAAFTNLTPEHMEEHKNMEAYYQAKKALFLNKRS
NSAVINIDTPYGIRLFKETGCSASVISENTKYGLDHKLFWQASVRRVGLSFGFTLISPSGYRVESSISLLGKAFALNTCM
AIVILCNLGIDIERIDSVLRKAGGLKMVLPGRMEVFQTGNSPRVIVDHGHTVDAVETALVAAKSITRGRLITIINADGQR
DPSKRKHLGQLCGAYSDKLFITDGHSRFENPAEIRRMILDGVEGPRKQVEQIPSMTQAVLAAIDIAKSDDTVLCSGFGDD
PYLDVLGKKIPYSLRDEVRRGLERFAQGT

Sequences:

>Translated_509_residues
MTLQGKPYRLSDLIKQTGVRLTDCSNQFSDRFVSGITQIAQSVERDDIFVAFQGKTRHGVEFLDQVQSCAAVLTDNKGRH
IMQSDCLPTRSTPILVTDSPRSDLIVLAKRVYPIDDIRIFGITGTNGKTSTMHIAAKLLEMMGISCGISTTIGSSASESD
SCLTTPELCQLYARIFTAKQARADFFALEASSHAINRGRLGDIVLEVAAFTNLTPEHMEEHKNMEAYYQAKKALFLNKRS
NSAVINIDTPYGIRLFKETGCSASVISENTKYGLDHKLFWQASVRRVGLSFGFTLISPSGYRVESSISLLGKAFALNTCM
AIVILCNLGIDIERIDSVLRKAGGLKMVLPGRMEVFQTGNSPRVIVDHGHTVDAVETALVAAKSITRGRLITIINADGQR
DPSKRKHLGQLCGAYSDKLFITDGHSRFENPAEIRRMILDGVEGPRKQVEQIPSMTQAVLAAIDIAKSDDTVLCSGFGDD
PYLDVLGKKIPYSLRDEVRRGLERFAQGT
>Mature_508_residues
TLQGKPYRLSDLIKQTGVRLTDCSNQFSDRFVSGITQIAQSVERDDIFVAFQGKTRHGVEFLDQVQSCAAVLTDNKGRHI
MQSDCLPTRSTPILVTDSPRSDLIVLAKRVYPIDDIRIFGITGTNGKTSTMHIAAKLLEMMGISCGISTTIGSSASESDS
CLTTPELCQLYARIFTAKQARADFFALEASSHAINRGRLGDIVLEVAAFTNLTPEHMEEHKNMEAYYQAKKALFLNKRSN
SAVINIDTPYGIRLFKETGCSASVISENTKYGLDHKLFWQASVRRVGLSFGFTLISPSGYRVESSISLLGKAFALNTCMA
IVILCNLGIDIERIDSVLRKAGGLKMVLPGRMEVFQTGNSPRVIVDHGHTVDAVETALVAAKSITRGRLITIINADGQRD
PSKRKHLGQLCGAYSDKLFITDGHSRFENPAEIRRMILDGVEGPRKQVEQIPSMTQAVLAAIDIAKSDDTVLCSGFGDDP
YLDVLGKKIPYSLRDEVRRGLERFAQGT

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=466, Percent_Identity=29.6137339055794, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_TROW8 (Q83HJ9)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789473.1
- ProteinModelPortal:   Q83HJ9
- SMR:   Q83HJ9
- STRING:   Q83HJ9
- GeneID:   1064748
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW545
- eggNOG:   COG0769
- HOGENOM:   HBG732588
- OMA:   GHEEGQT
- ProtClustDB:   CLSK229939
- BioCyc:   TWHI218496:TW0518-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.13

Molecular weight: Translated: 55909; Mature: 55778

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: BINDING 191-191 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQGKPYRLSDLIKQTGVRLTDCSNQFSDRFVSGITQIAQSVERDDIFVAFQGKTRHGV
CCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
EFLDQVQSCAAVLTDNKGRHIMQSDCLPTRSTPILVTDSPRSDLIVLAKRVYPIDDIRIF
HHHHHHHHHHHHHCCCCCCEEHHCCCCCCCCCCEEEECCCCCCCEEHHHHCCCCCCEEEE
GITGTNGKTSTMHIAAKLLEMMGISCGISTTIGSSASESDSCLTTPELCQLYARIFTAKQ
EEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ARADFFALEASSHAINRGRLGDIVLEVAAFTNLTPEHMEEHKNMEAYYQAKKALFLNKRS
HHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHEEECCC
NSAVINIDTPYGIRLFKETGCSASVISENTKYGLDHKLFWQASVRRVGLSFGFTLISPSG
CCEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCC
YRVESSISLLGKAFALNTCMAIVILCNLGIDIERIDSVLRKAGGLKMVLPGRMEVFQTGN
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCC
SPRVIVDHGHTVDAVETALVAAKSITRGRLITIINADGQRDPSKRKHLGQLCGAYSDKLF
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
ITDGHSRFENPAEIRRMILDGVEGPRKQVEQIPSMTQAVLAAIDIAKSDDTVLCSGFGDD
EECCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PYLDVLGKKIPYSLRDEVRRGLERFAQGT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLQGKPYRLSDLIKQTGVRLTDCSNQFSDRFVSGITQIAQSVERDDIFVAFQGKTRHGV
CCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
EFLDQVQSCAAVLTDNKGRHIMQSDCLPTRSTPILVTDSPRSDLIVLAKRVYPIDDIRIF
HHHHHHHHHHHHHCCCCCCEEHHCCCCCCCCCCEEEECCCCCCCEEHHHHCCCCCCEEEE
GITGTNGKTSTMHIAAKLLEMMGISCGISTTIGSSASESDSCLTTPELCQLYARIFTAKQ
EEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ARADFFALEASSHAINRGRLGDIVLEVAAFTNLTPEHMEEHKNMEAYYQAKKALFLNKRS
HHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHEEECCC
NSAVINIDTPYGIRLFKETGCSASVISENTKYGLDHKLFWQASVRRVGLSFGFTLISPSG
CCEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCC
YRVESSISLLGKAFALNTCMAIVILCNLGIDIERIDSVLRKAGGLKMVLPGRMEVFQTGN
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCC
SPRVIVDHGHTVDAVETALVAAKSITRGRLITIINADGQRDPSKRKHLGQLCGAYSDKLF
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
ITDGHSRFENPAEIRRMILDGVEGPRKQVEQIPSMTQAVLAAIDIAKSDDTVLCSGFGDD
EECCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PYLDVLGKKIPYSLRDEVRRGLERFAQGT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174