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The map label for this gene is glnA [H]

Identifier: 28572658

GI number: 28572658

Start: 547499

End: 548923

Strand: Direct

Name: glnA [H]

Synonym: TW509

Alternate gene names: 28572658

Gene position: 547499-548923 (Clockwise)

Preceding gene: 28572644

Following gene: 28572659

Centisome position: 59.13

GC content: 47.65

Gene sequence:

>1425_bases
GTGTTTGAAAATGCAAAAGAGGCAGCTGAGTATATAAAATCGAATCAAATTGAAGTGCTTGATGTTCGCTTCTGTGATTT
TCTCGGCGCTCAACATCATTTTGCCGTCCCTGCTCACGCAGTGGATGATGAGTTTTTTCAGTCACCCCAGGTCTTTGATG
CTTCCTCTCTGAGGGGCTATGCGGATATAAACCATTCAGATATGCTTCTTGTCGCAGATCCATCGACGTCCTATATTGAC
CCGTTTAGACACCACAAAACCCTTGCGATGATCTTTGATGTGCACGATCCACGCACGGGGGACGTTTATGAGCGCGACTC
ACGTAATGTGGCTAAAAAGGCAGAAAAGTACCTCGCGGGGTGCCGTTTTGCAGATACAGCGTATTTCGCAGCCGAAGCTG
AGTTTTTCATCTTTGATGACGTTCGGTTCAAGATCAGCAATCACTCATCATCCTATTCTGTTGATTCAGAGGAAGGTATT
TGGAACTCATCACGCAGGAAAGGGGGTGGGAACCTTGGTAATCAGCTCAAACACAAGGGAGGATACGCCCCCCTTCCCCC
TGCGGATCAACACGCCGACCTGCGCGATGACATAATGAATGCCCTAACAAAGGTTGGTCTGCGTGTTGAAAAAGGTCATC
ATGAAGCTGGCTCCGCGGGTCAAGGTGAGATAAATTACAGATTTAACACACTCGTCAATGCAGCAGATGATTTGCTGAAA
TTCAAATATGTCGTCAAAAACACAGCACATGAATGGGGAAAAAGCGCTACCTTTATGCCAAAGCCAATTGCCGGAGACAA
TGGGTCCGGGATGCACACTCATCAGTCACTGTGGAAAAATGGAAAACCCCTGTTCTACAGTGAAACCGGGTATGCCAATT
TGAGCGATATCGCCCTGTGGTACATAGGTGGCCTGCTAAAACATGGCCCTGCTCTAGCAGCATTTACGAACCCGTCAACG
AACTCATACAAGAGACTTGTTGCCGGATTTGAAGCACCAACCAGTCTCGTTTACTCTGCGGGCAACCGTTCGGCTGCTAT
CAGGATCCCCCCAACAATCGGAGCTGAAGGCAGTAAAAGAATTGAATACAGAGTGCCGGACTCCTCTTCAAATCCATATC
TCGCGTTTTCTGCCTTGCTTATGGCAGGAATAGACGGAATAAAGAATAAAATACACCCGGGCAGCCCGGTTGAGCAGAAT
ATGTACGAGCTCACAAAGGAGCAGTCAAGGGACCTGCGATATCTACCAAGCTCACTCAGCGATGCACTGCGTGCTCTTGA
GCAAGACTGTGATTTCCTCCTTGAGGGGAATGTATTCACCCGTGAATTCATTAGCGCATGGATTGATTACAAGAGGAAGA
ATGAGCTTAACCGGATTGCGCAGTATCCTCATCCACTTGAATTTGGGATGTATTATGGGGTGTGA

Upstream 100 bases:

>100_bases
GGACATATGAGGCCGTAACAGTGACAATCAAAAATAACAAACGTGTACAATACACAGGTGTGCACGCAAGGCGCGTGTGG
GACGTGGTCAGAGGTGCTGT

Downstream 100 bases:

>100_bases
TCTGTATGTCACGCAAACCTTCTCCAACCAGACGAACAAGGATACATCGCTTTCATAAAGGTTCCTGCGGCAGGAAGCTG
GTGGGTCTTACATGTTATGA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MFENAKEAAEYIKSNQIEVLDVRFCDFLGAQHHFAVPAHAVDDEFFQSPQVFDASSLRGYADINHSDMLLVADPSTSYID
PFRHHKTLAMIFDVHDPRTGDVYERDSRNVAKKAEKYLAGCRFADTAYFAAEAEFFIFDDVRFKISNHSSSYSVDSEEGI
WNSSRRKGGGNLGNQLKHKGGYAPLPPADQHADLRDDIMNALTKVGLRVEKGHHEAGSAGQGEINYRFNTLVNAADDLLK
FKYVVKNTAHEWGKSATFMPKPIAGDNGSGMHTHQSLWKNGKPLFYSETGYANLSDIALWYIGGLLKHGPALAAFTNPST
NSYKRLVAGFEAPTSLVYSAGNRSAAIRIPPTIGAEGSKRIEYRVPDSSSNPYLAFSALLMAGIDGIKNKIHPGSPVEQN
MYELTKEQSRDLRYLPSSLSDALRALEQDCDFLLEGNVFTREFISAWIDYKRKNELNRIAQYPHPLEFGMYYGV

Sequences:

>Translated_474_residues
MFENAKEAAEYIKSNQIEVLDVRFCDFLGAQHHFAVPAHAVDDEFFQSPQVFDASSLRGYADINHSDMLLVADPSTSYID
PFRHHKTLAMIFDVHDPRTGDVYERDSRNVAKKAEKYLAGCRFADTAYFAAEAEFFIFDDVRFKISNHSSSYSVDSEEGI
WNSSRRKGGGNLGNQLKHKGGYAPLPPADQHADLRDDIMNALTKVGLRVEKGHHEAGSAGQGEINYRFNTLVNAADDLLK
FKYVVKNTAHEWGKSATFMPKPIAGDNGSGMHTHQSLWKNGKPLFYSETGYANLSDIALWYIGGLLKHGPALAAFTNPST
NSYKRLVAGFEAPTSLVYSAGNRSAAIRIPPTIGAEGSKRIEYRVPDSSSNPYLAFSALLMAGIDGIKNKIHPGSPVEQN
MYELTKEQSRDLRYLPSSLSDALRALEQDCDFLLEGNVFTREFISAWIDYKRKNELNRIAQYPHPLEFGMYYGV
>Mature_474_residues
MFENAKEAAEYIKSNQIEVLDVRFCDFLGAQHHFAVPAHAVDDEFFQSPQVFDASSLRGYADINHSDMLLVADPSTSYID
PFRHHKTLAMIFDVHDPRTGDVYERDSRNVAKKAEKYLAGCRFADTAYFAAEAEFFIFDDVRFKISNHSSSYSVDSEEGI
WNSSRRKGGGNLGNQLKHKGGYAPLPPADQHADLRDDIMNALTKVGLRVEKGHHEAGSAGQGEINYRFNTLVNAADDLLK
FKYVVKNTAHEWGKSATFMPKPIAGDNGSGMHTHQSLWKNGKPLFYSETGYANLSDIALWYIGGLLKHGPALAAFTNPST
NSYKRLVAGFEAPTSLVYSAGNRSAAIRIPPTIGAEGSKRIEYRVPDSSSNPYLAFSALLMAGIDGIKNKIHPGSPVEQN
MYELTKEQSRDLRYLPSSLSDALRALEQDCDFLLEGNVFTREFISAWIDYKRKNELNRIAQYPHPLEFGMYYGV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=487, Percent_Identity=23.2032854209446, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=48.8322717622081, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI87081870, Length=214, Percent_Identity=33.6448598130841, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52909; Mature: 52909

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENAKEAAEYIKSNQIEVLDVRFCDFLGAQHHFAVPAHAVDDEFFQSPQVFDASSLRGY
CCCCHHHHHHHHHCCCEEEEEEHHHHHHCCCCCEECCCHHCCHHHHCCCCCCCHHHCCCE
ADINHSDMLLVADPSTSYIDPFRHHKTLAMIFDVHDPRTGDVYERDSRNVAKKAEKYLAG
ECCCCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
CRFADTAYFAAEAEFFIFDDVRFKISNHSSSYSVDSEEGIWNSSRRKGGGNLGNQLKHKG
CCCCCCCEEEECCEEEEEECEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
GYAPLPPADQHADLRDDIMNALTKVGLRVEKGHHEAGSAGQGEINYRFNTLVNAADDLLK
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
FKYVVKNTAHEWGKSATFMPKPIAGDNGSGMHTHQSLWKNGKPLFYSETGYANLSDIALW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHH
YIGGLLKHGPALAAFTNPSTNSYKRLVAGFEAPTSLVYSAGNRSAAIRIPPTIGAEGSKR
HHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCHHHEEECCCCEEEEECCCCCCCCCCCE
IEYRVPDSSSNPYLAFSALLMAGIDGIKNKIHPGSPVEQNMYELTKEQSRDLRYLPSSLS
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCHHHHH
DALRALEQDCDFLLEGNVFTREFISAWIDYKRKNELNRIAQYPHPLEFGMYYGV
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFENAKEAAEYIKSNQIEVLDVRFCDFLGAQHHFAVPAHAVDDEFFQSPQVFDASSLRGY
CCCCHHHHHHHHHCCCEEEEEEHHHHHHCCCCCEECCCHHCCHHHHCCCCCCCHHHCCCE
ADINHSDMLLVADPSTSYIDPFRHHKTLAMIFDVHDPRTGDVYERDSRNVAKKAEKYLAG
ECCCCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
CRFADTAYFAAEAEFFIFDDVRFKISNHSSSYSVDSEEGIWNSSRRKGGGNLGNQLKHKG
CCCCCCCEEEECCEEEEEECEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
GYAPLPPADQHADLRDDIMNALTKVGLRVEKGHHEAGSAGQGEINYRFNTLVNAADDLLK
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
FKYVVKNTAHEWGKSATFMPKPIAGDNGSGMHTHQSLWKNGKPLFYSETGYANLSDIALW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHH
YIGGLLKHGPALAAFTNPSTNSYKRLVAGFEAPTSLVYSAGNRSAAIRIPPTIGAEGSKR
HHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCHHHEEECCCCEEEEECCCCCCCCCCCE
IEYRVPDSSSNPYLAFSALLMAGIDGIKNKIHPGSPVEQNMYELTKEQSRDLRYLPSSLS
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCHHHHH
DALRALEQDCDFLLEGNVFTREFISAWIDYKRKNELNRIAQYPHPLEFGMYYGV
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099074 [H]