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The map label for this gene is pdxT

Identifier: 28572654

GI number: 28572654

Start: 543467

End: 544033

Strand: Reverse

Name: pdxT

Synonym: TW505

Alternate gene names: 28572654

Gene position: 544033-543467 (Counterclockwise)

Preceding gene: 28572655

Following gene: 28572653

Centisome position: 58.75

GC content: 46.56

Gene sequence:

>567_bases
ATGACCGTTGGAGTTCTCTCCCTCCAGGGAAGTTTTTATGAGCACCTATCTATTTTGAGCAGGCTAAACACTGACCACAT
TCAAGTAAAAACTTCTGAAGATCTTTCCCGGGTCACGCGACTTATAATTCCCGGTGGGGAGTCTACTGCTATGCTCGCTC
TGACCCAGAAGAGCGGCCTGTTTGATTTGGTGAGAGACCGCATCATGTCTGGCATGCCTGTGTACGGCACGTGTGCGGGC
ATGATTATGCTATCGACGTTTGTAGAAGATTTTCCTAACCAAAAGACTTTGTCTTGTCTTGATATTGCCGTTCGGCGCAA
TGCCTTTGGAAGGCAGATAAACAGTTTTGAGAGCGAAGTTTCCTTTCTAAACTCAAAAATTACTGTGCCTTTTATTCGTG
CGCCAAAGATTACTCAGATTGGTGAGGGCGTTGATGTTTTGTCTCGTCTCGAGTCGGGCGATATCGTTGCTGTAAGACAG
GGAAATGTCATGGCAACAGCATTTCATCCCGAGCTTACCGGGGGTGCAGCCGTGCATGAATATTTTTTACATCTGGGTCT
AGAATAG

Upstream 100 bases:

>100_bases
ACGATGATGCGGATCTCCTGGCCCGCGTGTCGCGTAATTTAGGTCAGGCTATGCCCGGTGTTTCCAATCTTGATGTGCGT
TTTTCGAGTCGGGGTGTGTA

Downstream 100 bases:

>100_bases
AGCTCAGGCTATTGGTATGTCATAATCGATACACGGTGATGTATGTCAGGACATTCAAAATGGGCAACAATAAAGCGCAA
AAAGGCGGTTAATGATGCTC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTVGVLSLQGSFYEHLSILSRLNTDHIQVKTSEDLSRVTRLIIPGGESTAMLALTQKSGLFDLVRDRIMSGMPVYGTCAG
MIMLSTFVEDFPNQKTLSCLDIAVRRNAFGRQINSFESEVSFLNSKITVPFIRAPKITQIGEGVDVLSRLESGDIVAVRQ
GNVMATAFHPELTGGAAVHEYFLHLGLE

Sequences:

>Translated_188_residues
MTVGVLSLQGSFYEHLSILSRLNTDHIQVKTSEDLSRVTRLIIPGGESTAMLALTQKSGLFDLVRDRIMSGMPVYGTCAG
MIMLSTFVEDFPNQKTLSCLDIAVRRNAFGRQINSFESEVSFLNSKITVPFIRAPKITQIGEGVDVLSRLESGDIVAVRQ
GNVMATAFHPELTGGAAVHEYFLHLGLE
>Mature_187_residues
TVGVLSLQGSFYEHLSILSRLNTDHIQVKTSEDLSRVTRLIIPGGESTAMLALTQKSGLFDLVRDRIMSGMPVYGTCAGM
IMLSTFVEDFPNQKTLSCLDIAVRRNAFGRQINSFESEVSFLNSKITVPFIRAPKITQIGEGVDVLSRLESGDIVAVRQG
NVMATAFHPELTGGAAVHEYFLHLGLE

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=203, Percent_Identity=38.9162561576355, Blast_Score=121, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6323995, Length=213, Percent_Identity=36.6197183098592, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321048, Length=213, Percent_Identity=36.150234741784, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_TROW8 (Q83HM6)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789434.1
- ProteinModelPortal:   Q83HM6
- SMR:   Q83HM6
- STRING:   Q83HM6
- GeneID:   1064709
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW505
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   Q83HM6
- ProtClustDB:   PRK13525
- BioCyc:   TWHI218496:TW0479-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20577; Mature: 20446

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 78-78 ACT_SITE 169-169 ACT_SITE 171-171 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGVLSLQGSFYEHLSILSRLNTDHIQVKTSEDLSRVTRLIIPGGESTAMLALTQKSGL
CCEEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHEEECCCCCEEEEEEECCCCH
FDLVRDRIMSGMPVYGTCAGMIMLSTFVEDFPNQKTLSCLDIAVRRNAFGRQINSFESEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SFLNSKITVPFIRAPKITQIGEGVDVLSRLESGDIVAVRQGNVMATAFHPELTGGAAVHE
HHHCCCCEEEEECCCCCHHHCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHHHHH
YFLHLGLE
HHHHHCCC
>Mature Secondary Structure 
TVGVLSLQGSFYEHLSILSRLNTDHIQVKTSEDLSRVTRLIIPGGESTAMLALTQKSGL
CEEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHEEECCCCCEEEEEEECCCCH
FDLVRDRIMSGMPVYGTCAGMIMLSTFVEDFPNQKTLSCLDIAVRRNAFGRQINSFESEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SFLNSKITVPFIRAPKITQIGEGVDVLSRLESGDIVAVRQGNVMATAFHPELTGGAAVHE
HHHCCCCEEEEECCCCCHHHCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHHHHH
YFLHLGLE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174