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The map label for this gene is relA [H]

Identifier: 28572645

GI number: 28572645

Start: 533898

End: 536120

Strand: Reverse

Name: relA [H]

Synonym: TW496

Alternate gene names: 28572645

Gene position: 536120-533898 (Counterclockwise)

Preceding gene: 28572646

Following gene: 28572643

Centisome position: 57.9

GC content: 44.22

Gene sequence:

>2223_bases
GTGCACAGGAGCTTTGTAATGAAAATAGCAAATATAAGTCTTTTCTCCAGGTTGTTTAGTCAGTATCGTACAACCGGCAT
AGATAATATTTTGAAAGTCATTGCAAAGCATCACCCAAAGGCTGATCTATCCGGGGTAACTTTGGCATACAAAATTGCCA
GTGAGTGTCATAAGGGTCAAAAGAGGTACTCTGGAGAAGAATATATTTCGCATCCAGTTGCGGTGGCGGAAATACTGTCA
GATCTTGGAATGGGCCCAAATGGTGTTTCCGCCGGACTTTTGCATGACACCGTTGAGGATACTGATTTCACAATAGACCA
AGTATCGGAAACTTTTGGCCAGGAAGTCGCTCTGTTAGTTGACGGGGTGACAAAGCTCGATCGCATGAAATACGGCGTAA
ATGTGCAGAGCGAGAGCTGGCGGAAGATGATTGTTGCGATTTCTAAGGACATAAGAGTTCTTGTTATAAAGCTTGCTGAC
AGACTGCATAATGCAAGAACTTGGGGTTTTGTTCCGATTGAATCGGCTAAGCGTAAGGCCCGTGAGACTTTGGAAATATA
TGCCCCATTATCTGGCCGACTCGGCATACAAAATATAAAAACTGAATTAGAAGATCTATCCTTTATGGTGCTTGAACCAA
AGATTTATCAGGAAATTCTGGAGCTTGTAAATAAACGTTCCCCCAGGCGAGAGAAACTTATAAATGAGGTTCGCTCGGGG
ATTGACGATGAATTGAAGCTTCTAAGAATTCGCGGTAAGGTATCGGGGAGGCCAAAGGAAATATATTCGATTTATCAAAA
AATGGTCTTGCGCGGTCATAGTTTCTCAGACATACAAGACTTAGTAGCTTTCCGAATCGTGGTTCCTACTCTGCGTGATT
GCTATGCAGTTTTGGGTAGTATGCACACACGGTGGACACCAATGCAGGGGAGATTTAAGGATTATATAGCAACCCCAAAG
TCGAATTTTTATCAGTCAATACACACAACAGTTATGCTTGATGGGGGGTTTCCGGTAGAAATACAGATCAGAACCCCAGA
AATGCATCGTCAGGCAGAATTTGGGATTGCTGCCCACTGGAAATACAAGGAAAAACTGCGATCTCCTACGGTTGCATTCA
GAAAAACTGCGGCACTTGATCATGAGTCTGTCTATATCTCGCGCATAGCCGACTGGCAGGAAAGCAGTGATTCAAACGAG
TTGCTCGAGAATATGCGCTATGAGTTGAGCGTAAAGGACATTTATGTTTTTACGCCTGCCGGCAAGCTTGTAGGACTTCC
AGCAGGATCAACACCTGTTGATTTTGCCTATGCCGTACATACCGAAGTTGGGCACAGAACTGTTGGCGCAAAGGTTAATT
CCAGTATGGTTTCACTGGACACACCGTTATCCACAGGCGATGTAGTAGAGGTACTGACGATTAAGTCTGCCAACTACTCT
CCAAACAAAAACTGGCTTGAGTTTGTAAAAAGCACAAGGGCGAGAAATAAGATAAAAAGTTTCTTTACTAAAAGCCGGAG
AGAAGAGGCAATTGAGCTGGGTAAAAACGCTCTTTTAAAATGCTTTAGAAAGCGCAATGTGTCCGTAAAATATATTCAAA
AGCGCCTACCCGAACTCCTGTCATCTCTGCGCTTTAGTGATATCAATGATGTCTACCGCGCTGTTGGCGAGGGAAGGCTT
AAAGCCTTGACCGTTGTTGACACCCTGTCCAAGCTGATCCATGACACCGTTGAGGATGAGACATCCCCGATACAGGTTAT
TAGAACAGACAAACCCGCAAACGAGCCATCTGTTCTGGTTCGGGGGATACATGATATCCTAGTGCGCCTTGCAAGGTGCT
GCACACCAGTTCCCGGCGATGAGATTATCGGTTATGTCACGAGAGGTACGGGGGTTTCGGTTCACATCATGACCTGCGGC
AATATCTCACATATGCCGGAGAAAGATAGCAGGTTGGTCAAAGTTGAATGGAGCCGTAGCGCCCAGACAAAGCCATTCAG
GGTGTCTATTCAAATTGATGGCCTAAACAGGCCCGACTTGATCAATGATATTACCAAGGTTTTTTCTGAATATCGTATAA
ATATCCTATCTGCTAATCTCTCAACAAATCAGCATAGGGCCAGTAATAAATTTGTATGCGAAGCCTATGACGTCTCGCGT
TTGGAAAAGGTCATGTCTGAAATACGGAAAATAGACTCTGTATACGATTGTTATAGAGCCTAA

Upstream 100 bases:

>100_bases
CAGGCTCAGACTATCTAAGTATGCAGGTAAATATAAATTACATGCATCTGTCGTATTGACTATTTTTCTGTGTTTTGCGG
GGTTCCTGAACAGATAAAAT

Downstream 100 bases:

>100_bases
AAACTGTGGCCGAAACCTCTTGTGACTGAAACCTCTATGGAAGGGTTTTTTTAAGGGGCCTTACTCTCCGGCAGTCGAGT
TTCTCAGGCGAGTGGCCACA

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MHRSFVMKIANISLFSRLFSQYRTTGIDNILKVIAKHHPKADLSGVTLAYKIASECHKGQKRYSGEEYISHPVAVAEILS
DLGMGPNGVSAGLLHDTVEDTDFTIDQVSETFGQEVALLVDGVTKLDRMKYGVNVQSESWRKMIVAISKDIRVLVIKLAD
RLHNARTWGFVPIESAKRKARETLEIYAPLSGRLGIQNIKTELEDLSFMVLEPKIYQEILELVNKRSPRREKLINEVRSG
IDDELKLLRIRGKVSGRPKEIYSIYQKMVLRGHSFSDIQDLVAFRIVVPTLRDCYAVLGSMHTRWTPMQGRFKDYIATPK
SNFYQSIHTTVMLDGGFPVEIQIRTPEMHRQAEFGIAAHWKYKEKLRSPTVAFRKTAALDHESVYISRIADWQESSDSNE
LLENMRYELSVKDIYVFTPAGKLVGLPAGSTPVDFAYAVHTEVGHRTVGAKVNSSMVSLDTPLSTGDVVEVLTIKSANYS
PNKNWLEFVKSTRARNKIKSFFTKSRREEAIELGKNALLKCFRKRNVSVKYIQKRLPELLSSLRFSDINDVYRAVGEGRL
KALTVVDTLSKLIHDTVEDETSPIQVIRTDKPANEPSVLVRGIHDILVRLARCCTPVPGDEIIGYVTRGTGVSVHIMTCG
NISHMPEKDSRLVKVEWSRSAQTKPFRVSIQIDGLNRPDLINDITKVFSEYRINILSANLSTNQHRASNKFVCEAYDVSR
LEKVMSEIRKIDSVYDCYRA

Sequences:

>Translated_740_residues
MHRSFVMKIANISLFSRLFSQYRTTGIDNILKVIAKHHPKADLSGVTLAYKIASECHKGQKRYSGEEYISHPVAVAEILS
DLGMGPNGVSAGLLHDTVEDTDFTIDQVSETFGQEVALLVDGVTKLDRMKYGVNVQSESWRKMIVAISKDIRVLVIKLAD
RLHNARTWGFVPIESAKRKARETLEIYAPLSGRLGIQNIKTELEDLSFMVLEPKIYQEILELVNKRSPRREKLINEVRSG
IDDELKLLRIRGKVSGRPKEIYSIYQKMVLRGHSFSDIQDLVAFRIVVPTLRDCYAVLGSMHTRWTPMQGRFKDYIATPK
SNFYQSIHTTVMLDGGFPVEIQIRTPEMHRQAEFGIAAHWKYKEKLRSPTVAFRKTAALDHESVYISRIADWQESSDSNE
LLENMRYELSVKDIYVFTPAGKLVGLPAGSTPVDFAYAVHTEVGHRTVGAKVNSSMVSLDTPLSTGDVVEVLTIKSANYS
PNKNWLEFVKSTRARNKIKSFFTKSRREEAIELGKNALLKCFRKRNVSVKYIQKRLPELLSSLRFSDINDVYRAVGEGRL
KALTVVDTLSKLIHDTVEDETSPIQVIRTDKPANEPSVLVRGIHDILVRLARCCTPVPGDEIIGYVTRGTGVSVHIMTCG
NISHMPEKDSRLVKVEWSRSAQTKPFRVSIQIDGLNRPDLINDITKVFSEYRINILSANLSTNQHRASNKFVCEAYDVSR
LEKVMSEIRKIDSVYDCYRA
>Mature_740_residues
MHRSFVMKIANISLFSRLFSQYRTTGIDNILKVIAKHHPKADLSGVTLAYKIASECHKGQKRYSGEEYISHPVAVAEILS
DLGMGPNGVSAGLLHDTVEDTDFTIDQVSETFGQEVALLVDGVTKLDRMKYGVNVQSESWRKMIVAISKDIRVLVIKLAD
RLHNARTWGFVPIESAKRKARETLEIYAPLSGRLGIQNIKTELEDLSFMVLEPKIYQEILELVNKRSPRREKLINEVRSG
IDDELKLLRIRGKVSGRPKEIYSIYQKMVLRGHSFSDIQDLVAFRIVVPTLRDCYAVLGSMHTRWTPMQGRFKDYIATPK
SNFYQSIHTTVMLDGGFPVEIQIRTPEMHRQAEFGIAAHWKYKEKLRSPTVAFRKTAALDHESVYISRIADWQESSDSNE
LLENMRYELSVKDIYVFTPAGKLVGLPAGSTPVDFAYAVHTEVGHRTVGAKVNSSMVSLDTPLSTGDVVEVLTIKSANYS
PNKNWLEFVKSTRARNKIKSFFTKSRREEAIELGKNALLKCFRKRNVSVKYIQKRLPELLSSLRFSDINDVYRAVGEGRL
KALTVVDTLSKLIHDTVEDETSPIQVIRTDKPANEPSVLVRGIHDILVRLARCCTPVPGDEIIGYVTRGTGVSVHIMTCG
NISHMPEKDSRLVKVEWSRSAQTKPFRVSIQIDGLNRPDLINDITKVFSEYRINILSANLSTNQHRASNKFVCEAYDVSR
LEKVMSEIRKIDSVYDCYRA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=721, Percent_Identity=38.4188626907073, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1789147, Length=737, Percent_Identity=33.5142469470828, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83800; Mature: 83800

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRSFVMKIANISLFSRLFSQYRTTGIDNILKVIAKHHPKADLSGVTLAYKIASECHKGQ
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRYSGEEYISHPVAVAEILSDLGMGPNGVSAGLLHDTVEDTDFTIDQVSETFGQEVALLV
HHCCCHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DGVTKLDRMKYGVNVQSESWRKMIVAISKDIRVLVIKLADRLHNARTWGFVPIESAKRKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHH
RETLEIYAPLSGRLGIQNIKTELEDLSFMVLEPKIYQEILELVNKRSPRREKLINEVRSG
HHHEEEEECCCCCCCHHHHHHHHHHCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCC
IDDELKLLRIRGKVSGRPKEIYSIYQKMVLRGHSFSDIQDLVAFRIVVPTLRDCYAVLGS
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
MHTRWTPMQGRFKDYIATPKSNFYQSIHTTVMLDGGFPVEIQIRTPEMHRQAEFGIAAHW
CCCCCCCCCCHHHHHHCCCCHHHHHHHCEEEEECCCCEEEEEEECCHHHHHHCCCEEEEC
KYKEKLRSPTVAFRKTAALDHESVYISRIADWQESSDSNELLENMRYELSVKDIYVFTPA
CHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCHHEEEEEEEEEECCC
GKLVGLPAGSTPVDFAYAVHTEVGHRTVGAKVNSSMVSLDTPLSTGDVVEVLTIKSANYS
CCEEECCCCCCCCCEEEEEEHHCCCEEECCCCCCCEEEECCCCCCCCEEEEEEEECCCCC
PNKNWLEFVKSTRARNKIKSFFTKSRREEAIELGKNALLKCFRKRNVSVKYIQKRLPELL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SSLRFSDINDVYRAVGEGRLKALTVVDTLSKLIHDTVEDETSPIQVIRTDKPANEPSVLV
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHH
RGIHDILVRLARCCTPVPGDEIIGYVTRGTGVSVHIMTCGNISHMPEKDSRLVKVEWSRS
HHHHHHHHHHHHHCCCCCCCHHEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCC
AQTKPFRVSIQIDGLNRPDLINDITKVFSEYRINILSANLSTNQHRASNKFVCEAYDVSR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEEEEHHHHHH
LEKVMSEIRKIDSVYDCYRA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHRSFVMKIANISLFSRLFSQYRTTGIDNILKVIAKHHPKADLSGVTLAYKIASECHKGQ
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRYSGEEYISHPVAVAEILSDLGMGPNGVSAGLLHDTVEDTDFTIDQVSETFGQEVALLV
HHCCCHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DGVTKLDRMKYGVNVQSESWRKMIVAISKDIRVLVIKLADRLHNARTWGFVPIESAKRKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHH
RETLEIYAPLSGRLGIQNIKTELEDLSFMVLEPKIYQEILELVNKRSPRREKLINEVRSG
HHHEEEEECCCCCCCHHHHHHHHHHCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCC
IDDELKLLRIRGKVSGRPKEIYSIYQKMVLRGHSFSDIQDLVAFRIVVPTLRDCYAVLGS
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
MHTRWTPMQGRFKDYIATPKSNFYQSIHTTVMLDGGFPVEIQIRTPEMHRQAEFGIAAHW
CCCCCCCCCCHHHHHHCCCCHHHHHHHCEEEEECCCCEEEEEEECCHHHHHHCCCEEEEC
KYKEKLRSPTVAFRKTAALDHESVYISRIADWQESSDSNELLENMRYELSVKDIYVFTPA
CHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCHHEEEEEEEEEECCC
GKLVGLPAGSTPVDFAYAVHTEVGHRTVGAKVNSSMVSLDTPLSTGDVVEVLTIKSANYS
CCEEECCCCCCCCCEEEEEEHHCCCEEECCCCCCCEEEECCCCCCCCEEEEEEEECCCCC
PNKNWLEFVKSTRARNKIKSFFTKSRREEAIELGKNALLKCFRKRNVSVKYIQKRLPELL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SSLRFSDINDVYRAVGEGRLKALTVVDTLSKLIHDTVEDETSPIQVIRTDKPANEPSVLV
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHH
RGIHDILVRLARCCTPVPGDEIIGYVTRGTGVSVHIMTCGNISHMPEKDSRLVKVEWSRS
HHHHHHHHHHHHHCCCCCCCHHEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCC
AQTKPFRVSIQIDGLNRPDLINDITKVFSEYRINILSANLSTNQHRASNKFVCEAYDVSR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEEEEHHHHHH
LEKVMSEIRKIDSVYDCYRA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8825780; 8631867; 12000953 [H]