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The map label for this gene is lepA

Identifier: 28572643

GI number: 28572643

Start: 531458

End: 533263

Strand: Reverse

Name: lepA

Synonym: TW494

Alternate gene names: 28572643

Gene position: 533263-531458 (Counterclockwise)

Preceding gene: 28572645

Following gene: 28572642

Centisome position: 57.59

GC content: 46.68

Gene sequence:

>1806_bases
ATGACTGATAGGGTTCTGCCTGAGCAAATACGTAATTTTGGGATTATTGCGCATGTAGATCACGGTAAATCAACTCTTGC
CGACCGCATACTTCAACTAACTGGCGCGGTTTCTGATCGGGATATGCGTGAACAGTATCTTGACCGGCTTTATATAGAGC
GCGAGCGGGGCATAACCATAAAAAGCCAGGCTGTTACGCTGCAGTGGGATTGCGATGCGACGCAATATGTCTTGAACATG
GTTGACACCCCCGGACATGTTGATTTTACATATGAAGTCTCTCGCTCTTTGGCCGCGTGTGAAGCGGCTGTTATTCTCGT
CGATGCCACCCAAGGGGTTGAAGCGCAAACCTTGGCCAATCTTCATCTTGCAATTGAGAATAATCTCTTGATAATACCCG
TTTTGAGCAAAGTTGATCTGCCTTCTGCCGACGTCGAAGGGGCAACGCTGGAGCTGTCAGAGGTTTTGGAATGTCGAGTA
GAAGACGTAATGCATGTATCGGGTAAAACAGGTTATGGCGTAAAAGAACTACTTGATCTAATTGTCAGAAAAGCTCCGCC
CCCGAAAGGGGATGTTACCTCTGCCCCTCGCGCGCTTATCTTTGATTCTATCTATGACTCGTACAGAGGGGTTGTAACGT
ATGTAAAAATGTGCGATGGAACGATACGTGTCGGTGACAAAATAAGAATGATGTCGACAGGCGCTGAGCATACCTTATTG
GAAGTGGGTGTTTCTAACCCCGAACCACAATCGCGCCACAGTCTCTCAGTTGGTGAAGTCGGTTATTTCATAACAGGCGT
CAAAGACGTCAGGAAATCCAGGGTTGGGGATACAATTACAACCGATACTCACACAGCTACAGTACCTTTGCCAGGCTACT
CAAATCCCAAGCCGATGGTGTTCTCAGGTATTTACCCGCTTAACGGAAACGAGTACTCTGCGCTTCGCGAAGGGCTTGAC
AGATTAAAACTCTCTGACGCATCGATTGTGTACACTCCAGAGACATCTGCTGCTCTTGGCTTTGGTTTTAGATGCGGATT
CTTGGGTCTCTTGCACATGGAGATAGTAAGTGAAAGACTTAACCGTGAATTTGGTCTTGCGACGATTTCAACGGCCCCCA
GTGTCGCTTACGAAATCACTCCTGATGGTGAAAAAACTTTGGTCGTTATGAACCCTAGTGACTTTCCATCTGGCAAAATA
AAAGAAATAAAAGAGCCAACCGTCAAGGTATCCATATTGTCTCCAAAGGAGTACATCGGTTCGATTATGGAACTCTGTCA
AGCACGACGTGGTGTTATGCAGGGTATTGAGTACTTTGGCTCAGTAAGAGCTGAACTTATTTACGTCATGCCCCTCGCTG
AAATAGTGTTTGATTTTTTTGACAGTTTAAAGAGTAGGACAAAAGGGTACGCTAGCTTTGACTATAATCCTGAAGGCAGT
CAGCCCGCCGACCTGGTAAAAGTTGATATCCTATTGCAAGGAAACAAGGTAGACGCATTTAGTGCAATAGTCCACAGCAG
TAAGGCGTACTCTTATGGGTCCAGTATTTCAAAGCGCTTGAGTGAGCTTATTCCACGACAACAGTTTGAGGTGCCTATTC
AGGCCGCGATTGGATCCAGAGTTGTAGCGCGTGAAACCATTCGTGCTGTTAGAAAGGATGTTGTGGCCAAGTGTTACGGT
GGTGACATTACTAGAAAACGCAAGTTGCTTGAGAGGCAGAAGCAGGGCAAGGCCCGCATGAAGCTGATTGGCAGGGTCGA
GGTTCCGCAGGAGGTATTTGTTGCAACGCTTTCATCGTACAAATAA

Upstream 100 bases:

>100_bases
GCCAGTCAGATCGGGCAAAACTATGCTATCAGATGTAATAATGGGCTATCAGATGTAATAATGACCCATGATCGCAACAA
TTTATGCATAGAATTTTTTT

Downstream 100 bases:

>100_bases
ACGCGGTGGCCTTATGTCACGGGCCTCGGCTAAATCATTTAGATTTGCCCCCGCACAGTATCCATGTGTTGGTGTGACAG
ATTGTCTGGGATCTCCAAAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MTDRVLPEQIRNFGIIAHVDHGKSTLADRILQLTGAVSDRDMREQYLDRLYIERERGITIKSQAVTLQWDCDATQYVLNM
VDTPGHVDFTYEVSRSLAACEAAVILVDATQGVEAQTLANLHLAIENNLLIIPVLSKVDLPSADVEGATLELSEVLECRV
EDVMHVSGKTGYGVKELLDLIVRKAPPPKGDVTSAPRALIFDSIYDSYRGVVTYVKMCDGTIRVGDKIRMMSTGAEHTLL
EVGVSNPEPQSRHSLSVGEVGYFITGVKDVRKSRVGDTITTDTHTATVPLPGYSNPKPMVFSGIYPLNGNEYSALREGLD
RLKLSDASIVYTPETSAALGFGFRCGFLGLLHMEIVSERLNREFGLATISTAPSVAYEITPDGEKTLVVMNPSDFPSGKI
KEIKEPTVKVSILSPKEYIGSIMELCQARRGVMQGIEYFGSVRAELIYVMPLAEIVFDFFDSLKSRTKGYASFDYNPEGS
QPADLVKVDILLQGNKVDAFSAIVHSSKAYSYGSSISKRLSELIPRQQFEVPIQAAIGSRVVARETIRAVRKDVVAKCYG
GDITRKRKLLERQKQGKARMKLIGRVEVPQEVFVATLSSYK

Sequences:

>Translated_601_residues
MTDRVLPEQIRNFGIIAHVDHGKSTLADRILQLTGAVSDRDMREQYLDRLYIERERGITIKSQAVTLQWDCDATQYVLNM
VDTPGHVDFTYEVSRSLAACEAAVILVDATQGVEAQTLANLHLAIENNLLIIPVLSKVDLPSADVEGATLELSEVLECRV
EDVMHVSGKTGYGVKELLDLIVRKAPPPKGDVTSAPRALIFDSIYDSYRGVVTYVKMCDGTIRVGDKIRMMSTGAEHTLL
EVGVSNPEPQSRHSLSVGEVGYFITGVKDVRKSRVGDTITTDTHTATVPLPGYSNPKPMVFSGIYPLNGNEYSALREGLD
RLKLSDASIVYTPETSAALGFGFRCGFLGLLHMEIVSERLNREFGLATISTAPSVAYEITPDGEKTLVVMNPSDFPSGKI
KEIKEPTVKVSILSPKEYIGSIMELCQARRGVMQGIEYFGSVRAELIYVMPLAEIVFDFFDSLKSRTKGYASFDYNPEGS
QPADLVKVDILLQGNKVDAFSAIVHSSKAYSYGSSISKRLSELIPRQQFEVPIQAAIGSRVVARETIRAVRKDVVAKCYG
GDITRKRKLLERQKQGKARMKLIGRVEVPQEVFVATLSSYK
>Mature_600_residues
TDRVLPEQIRNFGIIAHVDHGKSTLADRILQLTGAVSDRDMREQYLDRLYIERERGITIKSQAVTLQWDCDATQYVLNMV
DTPGHVDFTYEVSRSLAACEAAVILVDATQGVEAQTLANLHLAIENNLLIIPVLSKVDLPSADVEGATLELSEVLECRVE
DVMHVSGKTGYGVKELLDLIVRKAPPPKGDVTSAPRALIFDSIYDSYRGVVTYVKMCDGTIRVGDKIRMMSTGAEHTLLE
VGVSNPEPQSRHSLSVGEVGYFITGVKDVRKSRVGDTITTDTHTATVPLPGYSNPKPMVFSGIYPLNGNEYSALREGLDR
LKLSDASIVYTPETSAALGFGFRCGFLGLLHMEIVSERLNREFGLATISTAPSVAYEITPDGEKTLVVMNPSDFPSGKIK
EIKEPTVKVSILSPKEYIGSIMELCQARRGVMQGIEYFGSVRAELIYVMPLAEIVFDFFDSLKSRTKGYASFDYNPEGSQ
PADLVKVDILLQGNKVDAFSAIVHSSKAYSYGSSISKRLSELIPRQQFEVPIQAAIGSRVVARETIRAVRKDVVAKCYGG
DITRKRKLLERQKQGKARMKLIGRVEVPQEVFVATLSSYK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=48.5049833887043, Blast_Score=610, Evalue=1e-174,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=42.7480916030534, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI25306283, Length=154, Percent_Identity=43.5064935064935, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306287, Length=154, Percent_Identity=43.5064935064935, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI19923640, Length=154, Percent_Identity=43.5064935064935, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI18390331, Length=194, Percent_Identity=34.5360824742268, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=36.8055555555556, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=40.3669724770642, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=40.3669724770642, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=40.3669724770642, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI217272892, Length=135, Percent_Identity=34.8148148148148, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI217272894, Length=135, Percent_Identity=34.8148148148148, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI94966752, Length=93, Percent_Identity=35.4838709677419, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=49.8310810810811, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI48994988, Length=472, Percent_Identity=27.9661016949153, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789738, Length=140, Percent_Identity=39.2857142857143, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789559, Length=253, Percent_Identity=29.6442687747036, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790835, Length=137, Percent_Identity=30.6569343065693, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=39.9345335515548, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17556745, Length=456, Percent_Identity=24.5614035087719, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=37.593984962406, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=37.593984962406, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=148, Percent_Identity=36.4864864864865, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=39.8601398601399, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=180, Percent_Identity=30.5555555555556, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=296, Percent_Identity=27.7027027027027, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=42.0965058236273, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6323098, Length=189, Percent_Identity=35.4497354497354, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=44.6428571428571, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324707, Length=168, Percent_Identity=35.1190476190476, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=168, Percent_Identity=35.1190476190476, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=193, Percent_Identity=35.7512953367876, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322675, Length=167, Percent_Identity=31.1377245508982, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=260, Percent_Identity=26.5384615384615, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=43.0717863105175, Blast_Score=507, Evalue=1e-144,
Organism=Drosophila melanogaster, GI221458488, Length=159, Percent_Identity=40.8805031446541, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24582462, Length=138, Percent_Identity=40.5797101449275, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI28574573, Length=160, Percent_Identity=40, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585711, Length=172, Percent_Identity=33.1395348837209, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585713, Length=172, Percent_Identity=33.1395348837209, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585709, Length=172, Percent_Identity=33.1395348837209, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21357743, Length=163, Percent_Identity=33.1288343558282, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572034, Length=218, Percent_Identity=27.9816513761468, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_TROW8 (Q83NI1)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789423.1
- ProteinModelPortal:   Q83NI1
- SMR:   Q83NI1
- STRING:   Q83NI1
- GeneID:   1064698
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW494
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   TWHI218496:TW0468-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66232; Mature: 66101

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRVLPEQIRNFGIIAHVDHGKSTLADRILQLTGAVSDRDMREQYLDRLYIERERGITI
CCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEE
KSQAVTLQWDCDATQYVLNMVDTPGHVDFTYEVSRSLAACEAAVILVDATQGVEAQTLAN
EEEEEEEEECCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHCEEEEEEECCCCCCHHHHHE
LHLAIENNLLIIPVLSKVDLPSADVEGATLELSEVLECRVEDVMHVSGKTGYGVKELLDL
EEEEEECCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHEECCCCCCCHHHHHHH
IVRKAPPPKGDVTSAPRALIFDSIYDSYRGVVTYVKMCDGTIRVGDKIRMMSTGAEHTLL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCEEE
EVGVSNPEPQSRHSLSVGEVGYFITGVKDVRKSRVGDTITTDTHTATVPLPGYSNPKPMV
EECCCCCCCCCCCCCCHHCHHHHHHCHHHHHHHHCCCCEECCCEEEEEECCCCCCCCCEE
FSGIYPLNGNEYSALREGLDRLKLSDASIVYTPETSAALGFGFRCGFLGLLHMEIVSERL
EEEEECCCCCHHHHHHHHHHHHEECCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHH
NREFGLATISTAPSVAYEITPDGEKTLVVMNPSDFPSGKIKEIKEPTVKVSILSPKEYIG
CHHCCEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCEEEEEEECCHHHHH
SIMELCQARRGVMQGIEYFGSVRAELIYVMPLAEIVFDFFDSLKSRTKGYASFDYNPEGS
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
QPADLVKVDILLQGNKVDAFSAIVHSSKAYSYGSSISKRLSELIPRQQFEVPIQAAIGSR
CCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCH
VVARETIRAVRKDVVAKCYGGDITRKRKLLERQKQGKARMKLIGRVEVPQEVFVATLSSY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHCC
K
C
>Mature Secondary Structure 
TDRVLPEQIRNFGIIAHVDHGKSTLADRILQLTGAVSDRDMREQYLDRLYIERERGITI
CCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEE
KSQAVTLQWDCDATQYVLNMVDTPGHVDFTYEVSRSLAACEAAVILVDATQGVEAQTLAN
EEEEEEEEECCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHCEEEEEEECCCCCCHHHHHE
LHLAIENNLLIIPVLSKVDLPSADVEGATLELSEVLECRVEDVMHVSGKTGYGVKELLDL
EEEEEECCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHEECCCCCCCHHHHHHH
IVRKAPPPKGDVTSAPRALIFDSIYDSYRGVVTYVKMCDGTIRVGDKIRMMSTGAEHTLL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCEEE
EVGVSNPEPQSRHSLSVGEVGYFITGVKDVRKSRVGDTITTDTHTATVPLPGYSNPKPMV
EECCCCCCCCCCCCCCHHCHHHHHHCHHHHHHHHCCCCEECCCEEEEEECCCCCCCCCEE
FSGIYPLNGNEYSALREGLDRLKLSDASIVYTPETSAALGFGFRCGFLGLLHMEIVSERL
EEEEECCCCCHHHHHHHHHHHHEECCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHH
NREFGLATISTAPSVAYEITPDGEKTLVVMNPSDFPSGKIKEIKEPTVKVSILSPKEYIG
CHHCCEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCEEEEEEECCHHHHH
SIMELCQARRGVMQGIEYFGSVRAELIYVMPLAEIVFDFFDSLKSRTKGYASFDYNPEGS
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
QPADLVKVDILLQGNKVDAFSAIVHSSKAYSYGSSISKRLSELIPRQQFEVPIQAAIGSR
CCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCH
VVARETIRAVRKDVVAKCYGGDITRKRKLLERQKQGKARMKLIGRVEVPQEVFVATLSSY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12606174