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The map label for this gene is pyk [H]

Identifier: 28572631

GI number: 28572631

Start: 519320

End: 520735

Strand: Reverse

Name: pyk [H]

Synonym: TW482

Alternate gene names: 28572631

Gene position: 520735-519320 (Counterclockwise)

Preceding gene: 28572632

Following gene: 28572630

Centisome position: 56.24

GC content: 48.38

Gene sequence:

>1416_bases
ATGCGTAGGGCCAAGATTGTTGTTACTCTCGGTCCGGCAGTATCAGATCTTGATTCTGTGAGAGATCTTATATCCGCTGG
TGCCGACGTCTTTCGCATGAATCTCAGCCACGGATCGTTGGATTTTCACAGGGAGGTATTTCTGAATGTTAGGACGGCTG
CTGCTGAGCTAGACAGGCATATTGCTATATTTGCTGATCTTCAGGGTCCAAAAATACGTTTAGGGCGTTTTGCTTCTGGG
CCGTATACCCTACAAGAGGGTGATATCTTTACAATCACAACCGAGCAGGTGTTGGGAACGAAAGAGCTTTCCAGCACAAC
TTTGCCGACATTACCAGATAATGTCTCAGAGGGGGATTTGCTTTTAATTGATGATGGCAAAGTTGCCCTGAGGGCGATTG
GGAATGACGGGGTTCGGATCACTACACGTGTTGAAGTTCCGGGAAAGGTCTCGAATAATAAGGGTATTAACCTTCCGGGT
GTTGGAGTTAAAGTTCCGGCCTTATCTGAAAAGGATATTGACTCACTCCGCTGGGCCCTTTCTCTTCCAGTTGATATGAT
TGCCTTGAGTTTTGTGCGCTGCGCATCAGATGTGGATGCTGTGCACAAGGTTATGGATGAAGAGGGTATTCGCCTTCCTG
TTATTGCAAAGATAGAAAAGCCTCAAGCAGTATCTGCCATAGAGGAGATTGCACTGCGGTTTGATGCGATTATGGTAGCA
AGGGGTGATCTTGGGGTTGAACTGCCGCTTGAAGATGTGCCGGTAGCCCAGAAGGAAATTGTTAAGGTTGCACGCAGGTT
TGCTCGGCCGGTTATTATCGCAACCCAGATGCTTGAGTCAATGATCTCTTCCTCGCGGCCCACCAGGGCTGAGGCGAGTG
ATGTTGCAAACGCTGTTCTTGATGGAGCCGATGCACTGATGCTAAGCGGTGAGACAAGTGTTGGTGAGTGGCCCGTTGAA
GCTGTGCGGGTCATGTCGAGGGTTATTGAAAAAACAGAGGAAAAGGCAATTTCACAAATAGTCGGTTTTGGTACAACTCC
AAAAACACAGGGTGGTGCCCTCGCCTGGGCCGCAGCAAAGGTTGCTGAGTTTATCGATGCGCGTTATCTGTGTGTCTTTA
GTGAAAGTGGAGATTCAATAAGACGGGTGTCGCGACTGCGTCATGATATACCCATTATTGGATTTAGTCCGGTTGAGTCC
GTTGTCCGACAGGTCTCTCTCTCTTGGGGTGTTCGATGTTTTTTGATAGAGAGGCTCCATCAGACTGATCAGATGATGCG
TAAGGCTGAACATGTTCTCCTCACAAACAATTTGGCCGAGATTGGAGAGAAGATTGTGATTATTTCAGGATCACCAGTTG
GGGTGCCGGGGAGTACAAACCATATGAGAATCCACGAGCTTGGAAGCACGTCCTAG

Upstream 100 bases:

>100_bases
TTCCGTCCTAAGGAAATGGTTGGGGGTTCGAGTCCCTCCAGGGGCGCGTCGTTTTCTGTTCAGTTACCTGGGTGTTTTTA
GAGCGAACTAGTGGTTGTTT

Downstream 100 bases:

>100_bases
ATCTATAAAGACTGCTCAAAATACCCGAGTACAAGTGCGTCCGGGGTAAACTAAACAGTTTTCCGACACCTCGTATCTTT
GGCGCTTAAATCGAAATGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRRAKIVVTLGPAVSDLDSVRDLISAGADVFRMNLSHGSLDFHREVFLNVRTAAAELDRHIAIFADLQGPKIRLGRFASG
PYTLQEGDIFTITTEQVLGTKELSSTTLPTLPDNVSEGDLLLIDDGKVALRAIGNDGVRITTRVEVPGKVSNNKGINLPG
VGVKVPALSEKDIDSLRWALSLPVDMIALSFVRCASDVDAVHKVMDEEGIRLPVIAKIEKPQAVSAIEEIALRFDAIMVA
RGDLGVELPLEDVPVAQKEIVKVARRFARPVIIATQMLESMISSSRPTRAEASDVANAVLDGADALMLSGETSVGEWPVE
AVRVMSRVIEKTEEKAISQIVGFGTTPKTQGGALAWAAAKVAEFIDARYLCVFSESGDSIRRVSRLRHDIPIIGFSPVES
VVRQVSLSWGVRCFLIERLHQTDQMMRKAEHVLLTNNLAEIGEKIVIISGSPVGVPGSTNHMRIHELGSTS

Sequences:

>Translated_471_residues
MRRAKIVVTLGPAVSDLDSVRDLISAGADVFRMNLSHGSLDFHREVFLNVRTAAAELDRHIAIFADLQGPKIRLGRFASG
PYTLQEGDIFTITTEQVLGTKELSSTTLPTLPDNVSEGDLLLIDDGKVALRAIGNDGVRITTRVEVPGKVSNNKGINLPG
VGVKVPALSEKDIDSLRWALSLPVDMIALSFVRCASDVDAVHKVMDEEGIRLPVIAKIEKPQAVSAIEEIALRFDAIMVA
RGDLGVELPLEDVPVAQKEIVKVARRFARPVIIATQMLESMISSSRPTRAEASDVANAVLDGADALMLSGETSVGEWPVE
AVRVMSRVIEKTEEKAISQIVGFGTTPKTQGGALAWAAAKVAEFIDARYLCVFSESGDSIRRVSRLRHDIPIIGFSPVES
VVRQVSLSWGVRCFLIERLHQTDQMMRKAEHVLLTNNLAEIGEKIVIISGSPVGVPGSTNHMRIHELGSTS
>Mature_471_residues
MRRAKIVVTLGPAVSDLDSVRDLISAGADVFRMNLSHGSLDFHREVFLNVRTAAAELDRHIAIFADLQGPKIRLGRFASG
PYTLQEGDIFTITTEQVLGTKELSSTTLPTLPDNVSEGDLLLIDDGKVALRAIGNDGVRITTRVEVPGKVSNNKGINLPG
VGVKVPALSEKDIDSLRWALSLPVDMIALSFVRCASDVDAVHKVMDEEGIRLPVIAKIEKPQAVSAIEEIALRFDAIMVA
RGDLGVELPLEDVPVAQKEIVKVARRFARPVIIATQMLESMISSSRPTRAEASDVANAVLDGADALMLSGETSVGEWPVE
AVRVMSRVIEKTEEKAISQIVGFGTTPKTQGGALAWAAAKVAEFIDARYLCVFSESGDSIRRVSRLRHDIPIIGFSPVES
VVRQVSLSWGVRCFLIERLHQTDQMMRKAEHVLLTNNLAEIGEKIVIISGSPVGVPGSTNHMRIHELGSTS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=36.7556468172485, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=36.7556468172485, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=37.0672097759674, Blast_Score=275, Evalue=9e-74,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=36.6053169734151, Blast_Score=261, Evalue=9e-70,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=36.6053169734151, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=36.8055555555556, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=36.8055555555556, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=34.3220338983051, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=34.3220338983051, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=34.8837209302326, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=36.7303609341826, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=38.4453781512605, Blast_Score=289, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=35.8333333333333, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=35.8762886597938, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=35.8762886597938, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=35.8762886597938, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=35.8762886597938, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6319279, Length=431, Percent_Identity=36.6589327146172, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=32.3170731707317, Blast_Score=232, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=36.3265306122449, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=36.3265306122449, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=38.4428223844282, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=30.9278350515464, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24646914, Length=182, Percent_Identity=45.6043956043956, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50997; Mature: 50997

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAKIVVTLGPAVSDLDSVRDLISAGADVFRMNLSHGSLDFHREVFLNVRTAAAELDRH
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCE
IAIFADLQGPKIRLGRFASGPYTLQEGDIFTITTEQVLGTKELSSTTLPTLPDNVSEGDL
EEEEEECCCCEEEEEEECCCCEEEECCCEEEEEHHHHHCCHHHCCCCCCCCCCCCCCCCE
LLIDDGKVALRAIGNDGVRITTRVEVPGKVSNNKGINLPGVGVKVPALSEKDIDSLRWAL
EEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHH
SLPVDMIALSFVRCASDVDAVHKVMDEEGIRLPVIAKIEKPQAVSAIEEIALRFDAIMVA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEE
RGDLGVELPLEDVPVAQKEIVKVARRFARPVIIATQMLESMISSSRPTRAEASDVANAVL
ECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
DGADALMLSGETSVGEWPVEAVRVMSRVIEKTEEKAISQIVGFGTTPKTQGGALAWAAAK
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VAEFIDARYLCVFSESGDSIRRVSRLRHDIPIIGFSPVESVVRQVSLSWGVRCFLIERLH
HHHHHCCEEEEEECCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHH
QTDQMMRKAEHVLLTNNLAEIGEKIVIISGSPVGVPGSTNHMRIHELGSTS
HHHHHHHHHHHEEEECCHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MRRAKIVVTLGPAVSDLDSVRDLISAGADVFRMNLSHGSLDFHREVFLNVRTAAAELDRH
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCE
IAIFADLQGPKIRLGRFASGPYTLQEGDIFTITTEQVLGTKELSSTTLPTLPDNVSEGDL
EEEEEECCCCEEEEEEECCCCEEEECCCEEEEEHHHHHCCHHHCCCCCCCCCCCCCCCCE
LLIDDGKVALRAIGNDGVRITTRVEVPGKVSNNKGINLPGVGVKVPALSEKDIDSLRWAL
EEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHH
SLPVDMIALSFVRCASDVDAVHKVMDEEGIRLPVIAKIEKPQAVSAIEEIALRFDAIMVA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEE
RGDLGVELPLEDVPVAQKEIVKVARRFARPVIIATQMLESMISSSRPTRAEASDVANAVL
ECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
DGADALMLSGETSVGEWPVEAVRVMSRVIEKTEEKAISQIVGFGTTPKTQGGALAWAAAK
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VAEFIDARYLCVFSESGDSIRRVSRLRHDIPIIGFSPVESVVRQVSLSWGVRCFLIERLH
HHHHHCCEEEEEECCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHH
QTDQMMRKAEHVLLTNNLAEIGEKIVIISGSPVGVPGSTNHMRIHELGSTS
HHHHHHHHHHHEEEECCHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]