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The map label for this gene is wcaL [C]

Identifier: 28572610

GI number: 28572610

Start: 496780

End: 497955

Strand: Reverse

Name: wcaL [C]

Synonym: TW460

Alternate gene names: 28572610

Gene position: 497955-496780 (Counterclockwise)

Preceding gene: 28572611

Following gene: 28572609

Centisome position: 53.78

GC content: 47.02

Gene sequence:

>1176_bases
ATGGTGCACGGAAAGTCGCTTAGGGTTCTGATAGGAACAGAAACCTTTCCTCCTGAAGTTAATGGTGCGGCCAAATTCTC
CGGCAGGCTCGCTTCGTGTCTTATAGAACGGGGACATATTGTCAGGGTTGTTGCCCCTTCCAGAACTCGGGGAAGAGGCG
GCGAGTATCTCGAGGAAATTGAGGGAAATGCCGTTCTGGTGTATCGCTTGCCAAGTGTTAAATGGCCCGGTCATAGCTGG
TTTCGCTTTGCAAGTCCGCTGTTTGTTTCGAGATTTTGTTCGAAAATTATTGATGACTTTCAACCCGATGTGATTCATTA
TCAATCGCATTTTATGATCGGTCTTAGCCTGAGCAAAAAGGGCAGGGCGCGCGGTGTGCGCCTTGTTGGAACGAATCATT
TTATGCCTGATAATGTGATTCATTATTGCGAGTTTTTGCCAACTTTCTTGCATAAGTTTCTTTGGAAGGCAAACTGGCAT
TATGCATCTAGGTGTTTTGCACGGGCAGATGTTGTAACAAGTCCTACTCAAAGTGCGGCAAATTTTTTGGAACGATACAC
AACTATTGAGAATGTTGTTCCGATAAGCTGTGGTATAGACCTTTCAGGATATACCCCCTCTTTTGAAAGGGGCAGTGTCT
TACTCTATGTCGGCAGAATCACCCGCGAGAAACAGTTAGATGTTTTGATTCGGGCTTTTGCAAAGTTAAATAACAAGGAT
ATAACCCTAAAACTGGTTGGGATTGGTGAAGAAGTGGAACCCCTGCGCGCACTCGCATATTCCCTAGGGGTTTCGGACAG
AGTGGTTTTTTGTGGGTATCTGGAAGAAGGGGATCTCAGGAAGACGTATTCTGAAGCCTCGGTCTTTGTTATGCCATCTA
CTGCGGAACTTCAGTCAATTGCTACCATGGAGGCTATGGCCTCCGGCTTGCCAGTTGTTGGCGCTGACTCTATGGCTCTT
CCCCATCTTGTTCGTGACGGTGAAAATGGATATTTGTTTAGTCCAGGGGATCCAGATGATCTGGCCTCTAAGCTCACCGC
AGTTATCGCCCTTGATGATGCTGATTTTGCCGCGATGCAACGCAGGTCCTTGGAAATTGTTGCGGAACACGAAATAGACC
GCACAATAGATGCGTTTGAGCGGCTGTATGCAGGCTCTCAAGTATCCTGCAACTAG

Upstream 100 bases:

>100_bases
AGCTTCTATATTTTGTTGCTTAACCAAGCACCCGATTCTGGGTGTTTGAGTGTAGTATGTTCCTGTGTGTTTTTTCCGTA
AGGTCTTAGGACTTTAGTGC

Downstream 100 bases:

>100_bases
TCTATGTCTAGCAAGAGAACAGTTTTTATAACCGGGGGAAACCGGGGGATAGGTCGCGCAATTGCTGAAAAGTTCAAGAG
CTTCAACTACAATGTCGCGA

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MVHGKSLRVLIGTETFPPEVNGAAKFSGRLASCLIERGHIVRVVAPSRTRGRGGEYLEEIEGNAVLVYRLPSVKWPGHSW
FRFASPLFVSRFCSKIIDDFQPDVIHYQSHFMIGLSLSKKGRARGVRLVGTNHFMPDNVIHYCEFLPTFLHKFLWKANWH
YASRCFARADVVTSPTQSAANFLERYTTIENVVPISCGIDLSGYTPSFERGSVLLYVGRITREKQLDVLIRAFAKLNNKD
ITLKLVGIGEEVEPLRALAYSLGVSDRVVFCGYLEEGDLRKTYSEASVFVMPSTAELQSIATMEAMASGLPVVGADSMAL
PHLVRDGENGYLFSPGDPDDLASKLTAVIALDDADFAAMQRRSLEIVAEHEIDRTIDAFERLYAGSQVSCN

Sequences:

>Translated_391_residues
MVHGKSLRVLIGTETFPPEVNGAAKFSGRLASCLIERGHIVRVVAPSRTRGRGGEYLEEIEGNAVLVYRLPSVKWPGHSW
FRFASPLFVSRFCSKIIDDFQPDVIHYQSHFMIGLSLSKKGRARGVRLVGTNHFMPDNVIHYCEFLPTFLHKFLWKANWH
YASRCFARADVVTSPTQSAANFLERYTTIENVVPISCGIDLSGYTPSFERGSVLLYVGRITREKQLDVLIRAFAKLNNKD
ITLKLVGIGEEVEPLRALAYSLGVSDRVVFCGYLEEGDLRKTYSEASVFVMPSTAELQSIATMEAMASGLPVVGADSMAL
PHLVRDGENGYLFSPGDPDDLASKLTAVIALDDADFAAMQRRSLEIVAEHEIDRTIDAFERLYAGSQVSCN
>Mature_391_residues
MVHGKSLRVLIGTETFPPEVNGAAKFSGRLASCLIERGHIVRVVAPSRTRGRGGEYLEEIEGNAVLVYRLPSVKWPGHSW
FRFASPLFVSRFCSKIIDDFQPDVIHYQSHFMIGLSLSKKGRARGVRLVGTNHFMPDNVIHYCEFLPTFLHKFLWKANWH
YASRCFARADVVTSPTQSAANFLERYTTIENVVPISCGIDLSGYTPSFERGSVLLYVGRITREKQLDVLIRAFAKLNNKD
ITLKLVGIGEEVEPLRALAYSLGVSDRVVFCGYLEEGDLRKTYSEASVFVMPSTAELQSIATMEAMASGLPVVGADSMAL
PHLVRDGENGYLFSPGDPDDLASKLTAVIALDDADFAAMQRRSLEIVAEHEIDRTIDAFERLYAGSQVSCN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=305, Percent_Identity=24.9180327868852, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 43405; Mature: 43405

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHGKSLRVLIGTETFPPEVNGAAKFSGRLASCLIERGHIVRVVAPSRTRGRGGEYLEEI
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHH
EGNAVLVYRLPSVKWPGHSWFRFASPLFVSRFCSKIIDDFQPDVIHYQSHFMIGLSLSKK
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCC
GRARGVRLVGTNHFMPDNVIHYCEFLPTFLHKFLWKANWHYASRCFARADVVTSPTQSAA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
NFLERYTTIENVVPISCGIDLSGYTPSFERGSVLLYVGRITREKQLDVLIRAFAKLNNKD
HHHHHHHHHHHEEEEEECEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCC
ITLKLVGIGEEVEPLRALAYSLGVSDRVVFCGYLEEGDLRKTYSEASVFVMPSTAELQSI
EEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCEEEEECCHHHHHHH
ATMEAMASGLPVVGADSMALPHLVRDGENGYLFSPGDPDDLASKLTAVIALDDADFAAMQ
HHHHHHHCCCCEECCCCHHCCHHHCCCCCCEEECCCCHHHHHHHEEEEEEECCCHHHHHH
RRSLEIVAEHEIDRTIDAFERLYAGSQVSCN
HCCCEEEEHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVHGKSLRVLIGTETFPPEVNGAAKFSGRLASCLIERGHIVRVVAPSRTRGRGGEYLEEI
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHH
EGNAVLVYRLPSVKWPGHSWFRFASPLFVSRFCSKIIDDFQPDVIHYQSHFMIGLSLSKK
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCC
GRARGVRLVGTNHFMPDNVIHYCEFLPTFLHKFLWKANWHYASRCFARADVVTSPTQSAA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
NFLERYTTIENVVPISCGIDLSGYTPSFERGSVLLYVGRITREKQLDVLIRAFAKLNNKD
HHHHHHHHHHHEEEEEECEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCC
ITLKLVGIGEEVEPLRALAYSLGVSDRVVFCGYLEEGDLRKTYSEASVFVMPSTAELQSI
EEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCEEEEECCHHHHHHH
ATMEAMASGLPVVGADSMALPHLVRDGENGYLFSPGDPDDLASKLTAVIALDDADFAAMQ
HHHHHHHCCCCEECCCCHHCCHHHCCCCCCEEECCCCHHHHHHHEEEEEEECCCHHHHHH
RRSLEIVAEHEIDRTIDAFERLYAGSQVSCN
HCCCEEEEHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]