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The map label for this gene is cysS

Identifier: 28572575

GI number: 28572575

Start: 461063

End: 462463

Strand: Direct

Name: cysS

Synonym: TW423

Alternate gene names: 28572575

Gene position: 461063-462463 (Clockwise)

Preceding gene: 28572574

Following gene: 28572576

Centisome position: 49.79

GC content: 43.83

Gene sequence:

>1401_bases
ATGACGTTAAATTTATACGATACCCTCTCAAGACGCATTGTTACCCTAGACTGCAGGGATCGTGTGGAACTCTACGTTTG
CGGACCCACAGTACAATCACCTCCACATATTGGTCATATGCGATCGGGCGTAGTCTATGACTGTTTGCGACGGTGGTTGG
AATACAAAGGCCTTCCGGTTTTGTATGTACGCAATATAACCGATATAGACGATAAAATACTTGCCTCTGCGCGCAGTACT
GAAACAGGCGAAACTTGGTGGCAGATTGCCTACAGGGTCTCTGGATTGTTTAATGAAGCCTATAAGGCGTTGTTTGTAAA
GCCACCAGACTATGAGCCATTGGTAACAGCGCATATTCCGGACATAATAAAGGCAATAGAGATACTCATACAGAAAAACG
TCGCCTACAGGGCGATGGATGGTTCAGGTAACGTCTTTTTCAGTATTGATAAGCACCCAAGTTATGGTGAGCTAACTGAC
CAGAAAGATCTTTTAATCGATGATTGCATTACTCCTGGAAAGCGTGACCCCAGAGATTTTACTCTATGGAAAGAAAAAAA
AGACACAGATCCTGACTTGGCTTTTTGGGAGTCCCCCTGGGGTCCCGGAAGACCGGGCTGGCATATAGAATGCTCAGTTA
TGGCAACGAAGTACTTAGGCACTCGTTTTGCCATACACGGGGGTGGAGTTGACTTACGTTTCCCTCATCATGAAAATGAG
TTGGCTCAAGCCCGCGCTTTGGGAGCGCATTTTGCTGATATATGGATGCACACGGGGGCAGTCAATGTTGAAGGTATCAA
AATGTCTAAGTCTTTTGGTAACTCAGTTCTTGTTCAGGATGCTTTGTCAAAAGTGTCACCGTCTGCTTTGCGATACTATT
TTCTAACTGCACACTACCGCTCAACCCTTAATTACACGGAAAATTCTTTGTCGCAAGCCTGTAACACTTGGAATAAACTT
CAAGGATGTATATACCGAGTATATGACTACCTCGAACGCGAGGGTTATGAAAACGACTTCTGCGTTTCCCAATTGAATAC
GGATTTTTCTACCTCACTTGATAATGACCTAAATATACCCGAAGCTCTTGCTATTGTTCACAATAAGGTAAGAGAAATGA
ATCGCTTGGTTGATACACAGGCAGATATGCAATATTTGGGGAATACACTGTCAGAAGTTGTAGAGATGCTTTCTATTTTG
TGCCCAATTGATCATAAAATAACATACACCAGTGGAGCGCAGGATAATAGCAAAGCCTTGCTTCAAGTATTACTTGAAGC
AAGAGACAGGGCGCGCTCTAAGGGTGATTTTTATACTTCAGACCTTTTGCGAGAATTATTAGCCGAGGCGGATATAAGCG
TTTCAGACGGTCATGTGTCTTATGGTAGTCCGCGGGGATGA

Upstream 100 bases:

>100_bases
GATTTACCACCGATAATTCTTGTACAGATTAGTATTTGCTTTATAAGTGCAGTGAATTGGTATAGATCTGCTTTGTTCAT
TTGACATACTGTATCTTGTA

Downstream 100 bases:

>100_bases
GACGAAAAAACAGGTCAGTTTCTAGCTCTTCAAGAAGGTCCGGGTTACCAAACCAGATTGAGCACAATACGATTGTTGGA
ATAAATCCGGTATACGAGGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase 1; CysRS 1

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTLNLYDTLSRRIVTLDCRDRVELYVCGPTVQSPPHIGHMRSGVVYDCLRRWLEYKGLPVLYVRNITDIDDKILASARST
ETGETWWQIAYRVSGLFNEAYKALFVKPPDYEPLVTAHIPDIIKAIEILIQKNVAYRAMDGSGNVFFSIDKHPSYGELTD
QKDLLIDDCITPGKRDPRDFTLWKEKKDTDPDLAFWESPWGPGRPGWHIECSVMATKYLGTRFAIHGGGVDLRFPHHENE
LAQARALGAHFADIWMHTGAVNVEGIKMSKSFGNSVLVQDALSKVSPSALRYYFLTAHYRSTLNYTENSLSQACNTWNKL
QGCIYRVYDYLEREGYENDFCVSQLNTDFSTSLDNDLNIPEALAIVHNKVREMNRLVDTQADMQYLGNTLSEVVEMLSIL
CPIDHKITYTSGAQDNSKALLQVLLEARDRARSKGDFYTSDLLRELLAEADISVSDGHVSYGSPRG

Sequences:

>Translated_466_residues
MTLNLYDTLSRRIVTLDCRDRVELYVCGPTVQSPPHIGHMRSGVVYDCLRRWLEYKGLPVLYVRNITDIDDKILASARST
ETGETWWQIAYRVSGLFNEAYKALFVKPPDYEPLVTAHIPDIIKAIEILIQKNVAYRAMDGSGNVFFSIDKHPSYGELTD
QKDLLIDDCITPGKRDPRDFTLWKEKKDTDPDLAFWESPWGPGRPGWHIECSVMATKYLGTRFAIHGGGVDLRFPHHENE
LAQARALGAHFADIWMHTGAVNVEGIKMSKSFGNSVLVQDALSKVSPSALRYYFLTAHYRSTLNYTENSLSQACNTWNKL
QGCIYRVYDYLEREGYENDFCVSQLNTDFSTSLDNDLNIPEALAIVHNKVREMNRLVDTQADMQYLGNTLSEVVEMLSIL
CPIDHKITYTSGAQDNSKALLQVLLEARDRARSKGDFYTSDLLRELLAEADISVSDGHVSYGSPRG
>Mature_465_residues
TLNLYDTLSRRIVTLDCRDRVELYVCGPTVQSPPHIGHMRSGVVYDCLRRWLEYKGLPVLYVRNITDIDDKILASARSTE
TGETWWQIAYRVSGLFNEAYKALFVKPPDYEPLVTAHIPDIIKAIEILIQKNVAYRAMDGSGNVFFSIDKHPSYGELTDQ
KDLLIDDCITPGKRDPRDFTLWKEKKDTDPDLAFWESPWGPGRPGWHIECSVMATKYLGTRFAIHGGGVDLRFPHHENEL
AQARALGAHFADIWMHTGAVNVEGIKMSKSFGNSVLVQDALSKVSPSALRYYFLTAHYRSTLNYTENSLSQACNTWNKLQ
GCIYRVYDYLEREGYENDFCVSQLNTDFSTSLDNDLNIPEALAIVHNKVREMNRLVDTQADMQYLGNTLSEVVEMLSILC
PIDHKITYTSGAQDNSKALLQVLLEARDRARSKGDFYTSDLLRELLAEADISVSDGHVSYGSPRG

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=318, Percent_Identity=37.4213836477987, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI10835051, Length=392, Percent_Identity=33.6734693877551, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI21269877, Length=392, Percent_Identity=33.6734693877551, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI62240992, Length=392, Percent_Identity=33.6734693877551, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI303304987, Length=392, Percent_Identity=33.6734693877551, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786737, Length=472, Percent_Identity=38.3474576271186, Blast_Score=285, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI17509797, Length=266, Percent_Identity=35.3383458646617, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17509795, Length=266, Percent_Identity=35.3383458646617, Blast_Score=165, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6324082, Length=316, Percent_Identity=34.8101265822785, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI19922218, Length=496, Percent_Identity=31.6532258064516, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI19922364, Length=237, Percent_Identity=39.662447257384, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC1_TROW8 (Q83HS8)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789355.1
- ProteinModelPortal:   Q83HS8
- SMR:   Q83HS8
- STRING:   Q83HS8
- GeneID:   1064266
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW423
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   VVKIYCC
- ProtClustDB:   PRK00260
- BioCyc:   TWHI218496:TW0400-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52787; Mature: 52656

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNLYDTLSRRIVTLDCRDRVELYVCGPTVQSPPHIGHMRSGVVYDCLRRWLEYKGLPV
CEECHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCE
LYVRNITDIDDKILASARSTETGETWWQIAYRVSGLFNEAYKALFVKPPDYEPLVTAHIP
EEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEHHHH
DIIKAIEILIQKNVAYRAMDGSGNVFFSIDKHPSYGELTDQKDLLIDDCITPGKRDPRDF
HHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
TLWKEKKDTDPDLAFWESPWGPGRPGWHIECSVMATKYLGTRFAIHGGGVDLRFPHHENE
EEECCCCCCCCCCEEECCCCCCCCCCCEEEEEEEEHHHHCCEEEEECCCEEEECCCCCHH
LAQARALGAHFADIWMHTGAVNVEGIKMSKSFGNSVLVQDALSKVSPSALRYYFLTAHYR
HHHHHHHHHHHHHHHHHCCCCEECEEEECHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
STLNYTENSLSQACNTWNKLQGCIYRVYDYLEREGYENDFCVSQLNTDFSTSLDNDLNIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHCCCCCCCCCH
EALAIVHNKVREMNRLVDTQADMQYLGNTLSEVVEMLSILCPIDHKITYTSGAQDNSKAL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
LQVLLEARDRARSKGDFYTSDLLRELLAEADISVSDGHVSYGSPRG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
TLNLYDTLSRRIVTLDCRDRVELYVCGPTVQSPPHIGHMRSGVVYDCLRRWLEYKGLPV
EECHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCE
LYVRNITDIDDKILASARSTETGETWWQIAYRVSGLFNEAYKALFVKPPDYEPLVTAHIP
EEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEHHHH
DIIKAIEILIQKNVAYRAMDGSGNVFFSIDKHPSYGELTDQKDLLIDDCITPGKRDPRDF
HHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
TLWKEKKDTDPDLAFWESPWGPGRPGWHIECSVMATKYLGTRFAIHGGGVDLRFPHHENE
EEECCCCCCCCCCEEECCCCCCCCCCCEEEEEEEEHHHHCCEEEEECCCEEEECCCCCHH
LAQARALGAHFADIWMHTGAVNVEGIKMSKSFGNSVLVQDALSKVSPSALRYYFLTAHYR
HHHHHHHHHHHHHHHHHCCCCEECEEEECHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
STLNYTENSLSQACNTWNKLQGCIYRVYDYLEREGYENDFCVSQLNTDFSTSLDNDLNIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHCCCCCCCCCH
EALAIVHNKVREMNRLVDTQADMQYLGNTLSEVVEMLSILCPIDHKITYTSGAQDNSKAL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
LQVLLEARDRARSKGDFYTSDLLRELLAEADISVSDGHVSYGSPRG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174