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The map label for this gene is ispDF

Identifier: 28572574

GI number: 28572574

Start: 459710

End: 460978

Strand: Direct

Name: ispDF

Synonym: TW422

Alternate gene names: 28572574

Gene position: 459710-460978 (Clockwise)

Preceding gene: 28572573

Following gene: 28572575

Centisome position: 49.65

GC content: 45.55

Gene sequence:

>1269_bases
ATGGCAGTTGGATTGCTTTTGCTTGCGGCGGGTGTCGGTAGCAGGCTGTCTTCTTCTCTGCCGAAGGCATTTGTCTCAGT
TGGTGGTTTGGATTTAATACAATGGTGCCTAAAAAACTTGGGTCAGTTAAGAACAGCTTTGGAAGTTGTTGTCACTGTTC
CAAAGGGTTTTGTTGAGCTTTGCGAAAGAAATGTTTTACAGAGCCTAGGAACTCTTGAAAAGATTAAGATAGTAACTGGC
GGGGCAACTCGGCAGGATTCCGTTGGTCTTGGTATCAGGTATTTTTCTGCAAGGATAACAAAACTTCTTGTGCATGATGT
AGCTCGTGCATTTACACCTCCTGAGATTTATCTATCGGTTATTAAGCAACTTGAGACATCTAAAGCTGTTATTCCTGTAA
TTGCGATTGTCGATAGCATAAAGAAGGTAAATATGCAGGACGCATATCGAGAGGTTGCACGGGGCCCCGGTGAACCTTTT
CATACCAAGAGCGTGTTGCATCTTGACAGGAGAGAATTTTTTAGCGCACAGACCCCACAGGGCTTTGATCGCGCGCTGCT
TGAAGCTGCCCACGAGCGCTCTGTTGCTAGCAATGAACAGTTTGCCGATGACTCTGTAATGGTTGCCCAGATTGAAAAGG
ATATAACACTGATAAATGGACATGAGGCGAGTTTTAAGGTAACCAATCCGTGTGATTTGCAGCGCGCCGAATTTGCGGCA
TCAAGTCTGCTCTCTAAGTCGAATGTATCTCCTGTAAACATCTCGCAACCGCCGATCTCTGCTCTAAGCATGCCGTTACC
ACTCATTGGGGTTGGTATTGATTTTCATAAATTCATTCTGGACGAGTCCCCACTCTTCTTGGCTTGTCTAGAGTGGAAAA
ATTACAGAAGATTGCAAGGTCATAGTGATGGGGATGTTGTTGCGCATGCTTGTACAACGGCTCTTTTATCTGCCGCGAAT
ATGGGGGATATAGGAAGTGTCTTTGGTGTTGACCTGGCCGCAACAAAAGATGCATCCGGGGCTTATTTTTTGGAATCAAC
TAACAGACTTTTGGCAACAAATGGATTTTGTGTTCTAAATATTGCGGTGCAAGTTATTTCTAATACCCCGCGCCTTGCAG
ACAGGAGAGTCGAAGCAGAGCATGCAATTTCAGACTGTCTATCAGGCGCGCGAATCAGTCTTTCTTCTGTAACAACTGAT
GGCATGGGGTTTCTAGGTCGCGGTGAGGGGATTGGGGCAATTGCTGTTGCTCAGATTTACCACCGATAA

Upstream 100 bases:

>100_bases
CGCCCGCGGTATTTTAGTTGCAGAGTTAGCGCTTGCCCGTCACACAAACCAGGAAGATGCGGAAGCTTTGCTTGATGAAG
TGCTTGCTGAATAAGTCAGT

Downstream 100 bases:

>100_bases
TTCTTGTACAGATTAGTATTTGCTTTATAAGTGCAGTGAATTGGTATAGATCTGCTTTGTTCATTTGACATACTGTATCT
TGTAATGACGTTAAATTTAT

Product: terpenoid biosynthesis bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAVGLLLLAAGVGSRLSSSLPKAFVSVGGLDLIQWCLKNLGQLRTALEVVVTVPKGFVELCERNVLQSLGTLEKIKIVTG
GATRQDSVGLGIRYFSARITKLLVHDVARAFTPPEIYLSVIKQLETSKAVIPVIAIVDSIKKVNMQDAYREVARGPGEPF
HTKSVLHLDRREFFSAQTPQGFDRALLEAAHERSVASNEQFADDSVMVAQIEKDITLINGHEASFKVTNPCDLQRAEFAA
SSLLSKSNVSPVNISQPPISALSMPLPLIGVGIDFHKFILDESPLFLACLEWKNYRRLQGHSDGDVVAHACTTALLSAAN
MGDIGSVFGVDLAATKDASGAYFLESTNRLLATNGFCVLNIAVQVISNTPRLADRRVEAEHAISDCLSGARISLSSVTTD
GMGFLGRGEGIGAIAVAQIYHR

Sequences:

>Translated_422_residues
MAVGLLLLAAGVGSRLSSSLPKAFVSVGGLDLIQWCLKNLGQLRTALEVVVTVPKGFVELCERNVLQSLGTLEKIKIVTG
GATRQDSVGLGIRYFSARITKLLVHDVARAFTPPEIYLSVIKQLETSKAVIPVIAIVDSIKKVNMQDAYREVARGPGEPF
HTKSVLHLDRREFFSAQTPQGFDRALLEAAHERSVASNEQFADDSVMVAQIEKDITLINGHEASFKVTNPCDLQRAEFAA
SSLLSKSNVSPVNISQPPISALSMPLPLIGVGIDFHKFILDESPLFLACLEWKNYRRLQGHSDGDVVAHACTTALLSAAN
MGDIGSVFGVDLAATKDASGAYFLESTNRLLATNGFCVLNIAVQVISNTPRLADRRVEAEHAISDCLSGARISLSSVTTD
GMGFLGRGEGIGAIAVAQIYHR
>Mature_421_residues
AVGLLLLAAGVGSRLSSSLPKAFVSVGGLDLIQWCLKNLGQLRTALEVVVTVPKGFVELCERNVLQSLGTLEKIKIVTGG
ATRQDSVGLGIRYFSARITKLLVHDVARAFTPPEIYLSVIKQLETSKAVIPVIAIVDSIKKVNMQDAYREVARGPGEPFH
TKSVLHLDRREFFSAQTPQGFDRALLEAAHERSVASNEQFADDSVMVAQIEKDITLINGHEASFKVTNPCDLQRAEFAAS
SLLSKSNVSPVNISQPPISALSMPLPLIGVGIDFHKFILDESPLFLACLEWKNYRRLQGHSDGDVVAHACTTALLSAANM
GDIGSVFGVDLAATKDASGAYFLESTNRLLATNGFCVLNIAVQVISNTPRLADRRVEAEHAISDCLSGARISLSSVTTDG
MGFLGRGEGIGAIAVAQIYHR

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=151, Percent_Identity=35.0993377483444, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789104, Length=244, Percent_Identity=25.8196721311475, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_TROW8 (Q83NK3)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789354.1
- ProteinModelPortal:   Q83NK3
- SMR:   Q83NK3
- STRING:   Q83NK3
- GeneID:   1064265
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW422
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- ProtClustDB:   CLSK229737
- BioCyc:   TWHI218496:TW0399-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 45324; Mature: 45192

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGLLLLAAGVGSRLSSSLPKAFVSVGGLDLIQWCLKNLGQLRTALEVVVTVPKGFVEL
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
CERNVLQSLGTLEKIKIVTGGATRQDSVGLGIRYFSARITKLLVHDVARAFTPPEIYLSV
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IKQLETSKAVIPVIAIVDSIKKVNMQDAYREVARGPGEPFHTKSVLHLDRREFFSAQTPQ
HHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCC
GFDRALLEAAHERSVASNEQFADDSVMVAQIEKDITLINGHEASFKVTNPCDLQRAEFAA
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCEEEEECCCCCEEECCCCCCHHHHHHH
SSLLSKSNVSPVNISQPPISALSMPLPLIGVGIDFHKFILDESPLFLACLEWKNYRRLQG
HHHHHHCCCCCCCCCCCCHHHHCCCCCCEEECCHHHHHHHCCCCCEEEEECCCCHHHHCC
HSDGDVVAHACTTALLSAANMGDIGSVFGVDLAATKDASGAYFLESTNRLLATNGFCVLN
CCCCHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCEEEEECCCCEEEECCEEEEH
IAVQVISNTPRLADRRVEAEHAISDCLSGARISLSSVTTDGMGFLGRGEGIGAIAVAQIY
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCHHHHHHHH
HR
CC
>Mature Secondary Structure 
AVGLLLLAAGVGSRLSSSLPKAFVSVGGLDLIQWCLKNLGQLRTALEVVVTVPKGFVEL
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
CERNVLQSLGTLEKIKIVTGGATRQDSVGLGIRYFSARITKLLVHDVARAFTPPEIYLSV
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IKQLETSKAVIPVIAIVDSIKKVNMQDAYREVARGPGEPFHTKSVLHLDRREFFSAQTPQ
HHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCC
GFDRALLEAAHERSVASNEQFADDSVMVAQIEKDITLINGHEASFKVTNPCDLQRAEFAA
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCEEEEECCCCCEEECCCCCCHHHHHHH
SSLLSKSNVSPVNISQPPISALSMPLPLIGVGIDFHKFILDESPLFLACLEWKNYRRLQG
HHHHHHCCCCCCCCCCCCHHHHCCCCCCEEECCHHHHHHHCCCCCEEEEECCCCHHHHCC
HSDGDVVAHACTTALLSAANMGDIGSVFGVDLAATKDASGAYFLESTNRLLATNGFCVLN
CCCCHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCEEEEECCCCEEEECCEEEEH
IAVQVISNTPRLADRRVEAEHAISDCLSGARISLSSVTTDGMGFLGRGEGIGAIAVAQIY
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCHHHHHHHH
HR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174