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The map label for this gene is hemK [H]

Identifier: 28572488

GI number: 28572488

Start: 366577

End: 367434

Strand: Direct

Name: hemK [H]

Synonym: TW334

Alternate gene names: 28572488

Gene position: 366577-367434 (Clockwise)

Preceding gene: 28572487

Following gene: 28572489

Centisome position: 39.59

GC content: 47.32

Gene sequence:

>858_bases
TTGTTTAGAGATCTTCTGTTGTGGGGCACCCGGCGGCTTTCCAACTCGCTTTCGCCCAGGGTTGAATCTGAAATACTTCT
AAGCTTTGCTTGCAATATTTCGCGAGAATGTGTTATGCGGCACACCCTCCTATCAACGGGCAGTGTTAGCCCTATTGAAG
TGGAAAAATATAAGAAGCTTATCGCGCAACGTTCAGAGGGATATCCCGTACAGTATCTAGTCCGCACGTCTGGTTTTTAC
GAACATGATATTTTAGTGGGCCCTGGAGCTTTGGTTCCGCGCCCTGAAACGGAGATCCTGGTTCAAAGGGTGCTTACAGA
ATTGTGTACCACGGGAACACTGATCCGGTCTGTATGGGATTTGGGTACCGGAACTGGATGTATAACCCTCGCGCTTGCTT
CGCGTGCTACTGATATTGAATACTTGGCTGTGGATAAAAGTAATTCGGCTATACAGTGGGCTGAGAAGAATTTGCGCCAT
CTAAGGAATGTCACAATTCGCAAAGCCGACTTTACCGTTGATAGTGATTTGCTAGCTTTACTAAGCGAATTTGGCCCCCC
TGATGTTGTAGTTGCAAATCCTCCATATTTGCCCCAATCGGTGATGCATGAGAAATATGAGCCGTATATGGCACTCTGTG
GGGGAGGCCCTGAAGGTCTAGACCTTCTTCGGGCAGTTGCGAGGGCTTCTTCGATTGTCCTGGCTAATTCCGGGCTTTTG
TTCTTAGAACATTTACCCGATCAAAGTCAATCCCTACGCGTATCCCTTGAGGCCATGGGCTTTTGTGATATAGAGAGCTT
TTGTGATCTTAACGATAGGCTTCGCTTTTCGTCTGCAAAGATCATTAGGCTGGATTAG

Upstream 100 bases:

>100_bases
TAGAACGGGCTATAAAGCGTATAACCTTGACCAAGTGTTGAATGGTGATGTCTCTCAGCTTATTCGGTCATGTATAGAGG
CGGACACGGACGTTTAGATC

Downstream 100 bases:

>100_bases
CCGCATTGAGATCCATAAATTACAGTTTTCAATTAGGATGGAACAGTAATGCCACAGACTTTTAACTGCAACAAAGATCC
ACTGTCCGCTTTTCGCGCCG

Product: peptide chain release factor methyltransferase

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MFRDLLLWGTRRLSNSLSPRVESEILLSFACNISRECVMRHTLLSTGSVSPIEVEKYKKLIAQRSEGYPVQYLVRTSGFY
EHDILVGPGALVPRPETEILVQRVLTELCTTGTLIRSVWDLGTGTGCITLALASRATDIEYLAVDKSNSAIQWAEKNLRH
LRNVTIRKADFTVDSDLLALLSEFGPPDVVVANPPYLPQSVMHEKYEPYMALCGGGPEGLDLLRAVARASSIVLANSGLL
FLEHLPDQSQSLRVSLEAMGFCDIESFCDLNDRLRFSSAKIIRLD

Sequences:

>Translated_285_residues
MFRDLLLWGTRRLSNSLSPRVESEILLSFACNISRECVMRHTLLSTGSVSPIEVEKYKKLIAQRSEGYPVQYLVRTSGFY
EHDILVGPGALVPRPETEILVQRVLTELCTTGTLIRSVWDLGTGTGCITLALASRATDIEYLAVDKSNSAIQWAEKNLRH
LRNVTIRKADFTVDSDLLALLSEFGPPDVVVANPPYLPQSVMHEKYEPYMALCGGGPEGLDLLRAVARASSIVLANSGLL
FLEHLPDQSQSLRVSLEAMGFCDIESFCDLNDRLRFSSAKIIRLD
>Mature_285_residues
MFRDLLLWGTRRLSNSLSPRVESEILLSFACNISRECVMRHTLLSTGSVSPIEVEKYKKLIAQRSEGYPVQYLVRTSGFY
EHDILVGPGALVPRPETEILVQRVLTELCTTGTLIRSVWDLGTGTGCITLALASRATDIEYLAVDKSNSAIQWAEKNLRH
LRNVTIRKADFTVDSDLLALLSEFGPPDVVVANPPYLPQSVMHEKYEPYMALCGGGPEGLDLLRAVARASSIVLANSGLL
FLEHLPDQSQSLRVSLEAMGFCDIESFCDLNDRLRFSSAKIIRLD

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=214, Percent_Identity=30.8411214953271, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787463, Length=273, Percent_Identity=30.4029304029304, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87082085, Length=194, Percent_Identity=29.3814432989691, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324266, Length=165, Percent_Identity=30.9090909090909, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31645; Mature: 31645

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRDLLLWGTRRLSNSLSPRVESEILLSFACNISRECVMRHTLLSTGSVSPIEVEKYKKL
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHHHHHHH
IAQRSEGYPVQYLVRTSGFYEHDILVGPGALVPRPETEILVQRVLTELCTTGTLIRSVWD
HHHCCCCCCEEEEEECCCCEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGTGTGCITLALASRATDIEYLAVDKSNSAIQWAEKNLRHLRNVTIRKADFTVDSDLLAL
CCCCCCCEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
LSEFGPPDVVVANPPYLPQSVMHEKYEPYMALCGGGPEGLDLLRAVARASSIVLANSGLL
HHHCCCCCEEECCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCEE
FLEHLPDQSQSLRVSLEAMGFCDIESFCDLNDRLRFSSAKIIRLD
EEECCCCCCCCEEEEEECCCCCCHHHHCCCCCCEEECCCEEEEEC
>Mature Secondary Structure
MFRDLLLWGTRRLSNSLSPRVESEILLSFACNISRECVMRHTLLSTGSVSPIEVEKYKKL
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHHHHHHH
IAQRSEGYPVQYLVRTSGFYEHDILVGPGALVPRPETEILVQRVLTELCTTGTLIRSVWD
HHHCCCCCCEEEEEECCCCEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGTGTGCITLALASRATDIEYLAVDKSNSAIQWAEKNLRHLRNVTIRKADFTVDSDLLAL
CCCCCCCEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
LSEFGPPDVVVANPPYLPQSVMHEKYEPYMALCGGGPEGLDLLRAVARASSIVLANSGLL
HHHCCCCCEEECCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCEE
FLEHLPDQSQSLRVSLEAMGFCDIESFCDLNDRLRFSSAKIIRLD
EEECCCCCCCCEEEEEECCCCCCHHHHCCCCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]