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The map label for this gene is groEL

Identifier: 28572481

GI number: 28572481

Start: 357802

End: 359424

Strand: Direct

Name: groEL

Synonym: TW327

Alternate gene names: 28572481

Gene position: 357802-359424 (Clockwise)

Preceding gene: 28572479

Following gene: 28572483

Centisome position: 38.64

GC content: 51.14

Gene sequence:

>1623_bases
ATGGCTAAAAAAATCACTTTCAACGAGGATGCTAGACGTGGTCTTGAGCGTGGTCTCAACACATTGGCTGATACTGTAAA
GGTCACCCTCGGGCCCAGGGGTCGCAATGTAGTTCTGGAAAAGAAGTGGGGTGCACCTGTAATAACAAATGACGGTGTGA
CAATCGCCAAGGAGATCGAGCTAGATGATCCGTACGAGAAGATTGGTGCAGAGCTTGTCAAGGAGGTTGCCAAGAAGACT
GATGATGTTGCAGGTGACGGGACCACAACATCTGTTGTATTAGCCCAGGCTATGGTACGTGAGGGCCTCAAAAACGTGGC
TGCTGGTGCTGATCCTATCAGTTTGCGCCGGGGTATTGAGAAGTCTGTTGCTGCGGTATCGAAAGCGCTTCTCACCTCCG
CGAAAGAGGTTGAGACTGAGGCAGAAATCGCTGCCTGCGCCTCGATCTCTGCCGGGGATCCGCAGATAGGGGATATTATT
GCCCAAGCACTCGAGAAGGTTGGCAAGGAAGGCGTTGTCACTGTCGAGGAGTCAAATACTTTCGGGACCGAACTCGAGAT
AACCGAGGGCATGCGTTTTGATAAAGGCTACCTTTCGGCTTATTTTGTAACCGATGCGGAGCGGCAGGAAACGGTTTTTG
AGAACCCTTACATTCTTATTTGTGATAGCAAAATATCGAGTGTTAAAGATCTGCTCCCGGTTGTTGACAAGGTTATCCAG
TCTGGTAAGCAACTTCTTATCATTGCTGAAGATGTCGACGGCGAGGCGCTTGCAACGCTCGTTGTTAACAAGATCCGCGG
AATTTTCAAGTCTGTTGCCGTTAAGGCTCCCGGTTTCGGTGACCGTAGGAAGATGATGTTACAGGATATTGCCGTTCTTA
CAGGCGGTCAGGTCATTTCTGAGGAGGTCGGTCTAAAGCTCGAGAATGCCACTCTTGATCTTCTGGGTTGTGCCCGTAAG
GTTGTTGTTAGCAAGGATGAGACGACAATTGTCGATGGTGCAGGGTCGTCTGATCAGATTGCTGGCCGCGTTTCGCAGAT
CCGGAAAGAGCTTGAGAATAGCGATAGTGACTATGACAGGGAAAAGCTTCAGGAGCGCCTTGCTAAGCTATCGGGCGGTG
TTGCTGTCATTCGTTCCGGTGCAGCGACTGAGGTTGAATTGAAAGAACGGAAACATCGCATTGAGGATGCGGTGCGTAAT
GCAAAAGCTGCTGTAGAAGAGGGGATTGTTGCAGGCGGTGGTGCTGCGCTTCTCCAGTCTGGCACTTCTGCCTTGAAGGA
TTTGCAGCTCACCTCGGAGGAGGCGGTTGGCCGAAATATCGTTCGTTCGGCTATAGAGGCGCCCCTCAGGCAGATATCGT
TGAATGCTGGTCTTGAGCCTGGTGTTGTTGTTGGTAAGGTATCTTCTTTACCTCAGGGTCACGGTCTTGATGCATCAACG
GGTGAGTACGTTGATATGCTTTCTAGGGGTATTTCAGATCCGGTAAAGGTTACGCGCTCTGCTCTTGAGAACGCTGCTTC
TATAGCTGGGCTTTTTCTGACTACCGAAGCCGTTGTTGCCGAGAAGCCTGAGCCAAAGCCTGCTCCTGGCCCGGCCGATC
CCGGTGCCGGGATGGATTTCTAA

Upstream 100 bases:

>100_bases
GTTTGCCTCAAGAATTGTCAACATTACCTCCTCTATAGTCATGTTGTGGGTAGGTTATACTTAAACCCGGATTTGTTCGG
TTTTCCAAGGAACGGAGATT

Downstream 100 bases:

>100_bases
ATCCTGTTTTGCCTGACTAGCGGCACGCACTTCTGTGTGCCGCTATTGTCTTGTGTCTCTTTTATGTCATTCTCTTGCAG
AACTGTGATTTTGTGCTTAT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAKKITFNEDARRGLERGLNTLADTVKVTLGPRGRNVVLEKKWGAPVITNDGVTIAKEIELDDPYEKIGAELVKEVAKKT
DDVAGDGTTTSVVLAQAMVREGLKNVAAGADPISLRRGIEKSVAAVSKALLTSAKEVETEAEIAACASISAGDPQIGDII
AQALEKVGKEGVVTVEESNTFGTELEITEGMRFDKGYLSAYFVTDAERQETVFENPYILICDSKISSVKDLLPVVDKVIQ
SGKQLLIIAEDVDGEALATLVVNKIRGIFKSVAVKAPGFGDRRKMMLQDIAVLTGGQVISEEVGLKLENATLDLLGCARK
VVVSKDETTIVDGAGSSDQIAGRVSQIRKELENSDSDYDREKLQERLAKLSGGVAVIRSGAATEVELKERKHRIEDAVRN
AKAAVEEGIVAGGGAALLQSGTSALKDLQLTSEEAVGRNIVRSAIEAPLRQISLNAGLEPGVVVGKVSSLPQGHGLDAST
GEYVDMLSRGISDPVKVTRSALENAASIAGLFLTTEAVVAEKPEPKPAPGPADPGAGMDF

Sequences:

>Translated_540_residues
MAKKITFNEDARRGLERGLNTLADTVKVTLGPRGRNVVLEKKWGAPVITNDGVTIAKEIELDDPYEKIGAELVKEVAKKT
DDVAGDGTTTSVVLAQAMVREGLKNVAAGADPISLRRGIEKSVAAVSKALLTSAKEVETEAEIAACASISAGDPQIGDII
AQALEKVGKEGVVTVEESNTFGTELEITEGMRFDKGYLSAYFVTDAERQETVFENPYILICDSKISSVKDLLPVVDKVIQ
SGKQLLIIAEDVDGEALATLVVNKIRGIFKSVAVKAPGFGDRRKMMLQDIAVLTGGQVISEEVGLKLENATLDLLGCARK
VVVSKDETTIVDGAGSSDQIAGRVSQIRKELENSDSDYDREKLQERLAKLSGGVAVIRSGAATEVELKERKHRIEDAVRN
AKAAVEEGIVAGGGAALLQSGTSALKDLQLTSEEAVGRNIVRSAIEAPLRQISLNAGLEPGVVVGKVSSLPQGHGLDAST
GEYVDMLSRGISDPVKVTRSALENAASIAGLFLTTEAVVAEKPEPKPAPGPADPGAGMDF
>Mature_539_residues
AKKITFNEDARRGLERGLNTLADTVKVTLGPRGRNVVLEKKWGAPVITNDGVTIAKEIELDDPYEKIGAELVKEVAKKTD
DVAGDGTTTSVVLAQAMVREGLKNVAAGADPISLRRGIEKSVAAVSKALLTSAKEVETEAEIAACASISAGDPQIGDIIA
QALEKVGKEGVVTVEESNTFGTELEITEGMRFDKGYLSAYFVTDAERQETVFENPYILICDSKISSVKDLLPVVDKVIQS
GKQLLIIAEDVDGEALATLVVNKIRGIFKSVAVKAPGFGDRRKMMLQDIAVLTGGQVISEEVGLKLENATLDLLGCARKV
VVSKDETTIVDGAGSSDQIAGRVSQIRKELENSDSDYDREKLQERLAKLSGGVAVIRSGAATEVELKERKHRIEDAVRNA
KAAVEEGIVAGGGAALLQSGTSALKDLQLTSEEAVGRNIVRSAIEAPLRQISLNAGLEPGVVVGKVSSLPQGHGLDASTG
EYVDMLSRGISDPVKVTRSALENAASIAGLFLTTEAVVAEKPEPKPAPGPADPGAGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=45.7627118644068, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=45.7627118644068, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=57.0888468809074, Blast_Score=578, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=45.4545454545455, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI193210679, Length=214, Percent_Identity=46.7289719626168, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17564182, Length=561, Percent_Identity=23.5294117647059, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=48.8549618320611, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322524, Length=109, Percent_Identity=38.5321100917431, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24641193, Length=526, Percent_Identity=45.4372623574144, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24641191, Length=526, Percent_Identity=45.4372623574144, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=44.7619047619048, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=44.7619047619048, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=44.7619047619048, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864606, Length=542, Percent_Identity=38.7453874538745, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=33.3333333333333, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=33.3333333333333, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_TROW8 (Q83NN0)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789261.1
- ProteinModelPortal:   Q83NN0
- SMR:   Q83NN0
- STRING:   Q83NN0
- GeneID:   1064614
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW327
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   MAAKEIL
- ProtClustDB:   PRK00013
- BioCyc:   TWHI218496:TW0306-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 56961; Mature: 56830

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKITFNEDARRGLERGLNTLADTVKVTLGPRGRNVVLEKKWGAPVITNDGVTIAKEIE
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEECCCEEEEEECC
LDDPYEKIGAELVKEVAKKTDDVAGDGTTTSVVLAQAMVREGLKNVAAGADPISLRRGIE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KSVAAVSKALLTSAKEVETEAEIAACASISAGDPQIGDIIAQALEKVGKEGVVTVEESNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
FGTELEITEGMRFDKGYLSAYFVTDAERQETVFENPYILICDSKISSVKDLLPVVDKVIQ
CCCEEEECCCCEECCCCEEEEEEECCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
SGKQLLIIAEDVDGEALATLVVNKIRGIFKSVAVKAPGFGDRRKMMLQDIAVLTGGQVIS
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHH
EEVGLKLENATLDLLGCARKVVVSKDETTIVDGAGSSDQIAGRVSQIRKELENSDSDYDR
HHHCCEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHH
EKLQERLAKLSGGVAVIRSGAATEVELKERKHRIEDAVRNAKAAVEEGIVAGGGAALLQS
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GTSALKDLQLTSEEAVGRNIVRSAIEAPLRQISLNAGLEPGVVVGKVSSLPQGHGLDAST
CHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCC
GEYVDMLSRGISDPVKVTRSALENAASIAGLFLTTEAVVAEKPEPKPAPGPADPGAGMDF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKITFNEDARRGLERGLNTLADTVKVTLGPRGRNVVLEKKWGAPVITNDGVTIAKEIE
CCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEECCCEEEEEECC
LDDPYEKIGAELVKEVAKKTDDVAGDGTTTSVVLAQAMVREGLKNVAAGADPISLRRGIE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KSVAAVSKALLTSAKEVETEAEIAACASISAGDPQIGDIIAQALEKVGKEGVVTVEESNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
FGTELEITEGMRFDKGYLSAYFVTDAERQETVFENPYILICDSKISSVKDLLPVVDKVIQ
CCCEEEECCCCEECCCCEEEEEEECCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
SGKQLLIIAEDVDGEALATLVVNKIRGIFKSVAVKAPGFGDRRKMMLQDIAVLTGGQVIS
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHH
EEVGLKLENATLDLLGCARKVVVSKDETTIVDGAGSSDQIAGRVSQIRKELENSDSDYDR
HHHCCEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHH
EKLQERLAKLSGGVAVIRSGAATEVELKERKHRIEDAVRNAKAAVEEGIVAGGGAALLQS
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GTSALKDLQLTSEEAVGRNIVRSAIEAPLRQISLNAGLEPGVVVGKVSSLPQGHGLDAST
CHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCC
GEYVDMLSRGISDPVKVTRSALENAASIAGLFLTTEAVVAEKPEPKPAPGPADPGAGMDF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174