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The map label for this gene is hom [H]

Identifier: 28572483

GI number: 28572483

Start: 360443

End: 361912

Strand: Direct

Name: hom [H]

Synonym: TW329

Alternate gene names: 28572483

Gene position: 360443-361912 (Clockwise)

Preceding gene: 28572481

Following gene: 28572484

Centisome position: 38.93

GC content: 46.19

Gene sequence:

>1470_bases
ATGCCTCTTCGAAACTCAAAGATTTCGGGTTTAATCACCAAACAAAGTAGCCTTGGATCAGTATACATATTGTATAACCG
GGTTTTAGGGGCTTGTGTGGATTATAGGAGTCTAAAACTTGCACTATTCGGTGCTGGTCGGGTTGGCTCCTGCGTTGCAC
GTTTCCTGCTGAGCAATGCAGATGAACTAAAAATACGCACCGGGGGACTAGATATAGAGCTCATTGGGATCGGCGTAAAG
GATTTGAGCAAAAGCCGTGAGTTGGATGCTTCGTACTATACCGATGATTTAGAGGGATTAATTCTCCGCGCGGATGTAGT
GATTGAGTTGATAGGTGGCCTCCATCCTGCAAAGGAGCTTATAACAAGGGCTTTACAAGTAGGTGCAGATGTTGTAAGTG
CAAATAAGACCCTTATCTCTGAGCATGGCCGTGAGCTTCTGGCATTGGCATCCAGCGTTGGCGCACAGTTTCTGTATGAA
GCCGCTGTTTGTGCGGCAATTCCTGTTATACGCCCTCTTAAAGATTCTTTGAGTGGTGATGTTATACAAAAAATTTCGGG
CATTGTAAACGGGACAACCAATTTTATTCTTGACAGAATGGAATCAACAGGGGATTCCCTCGAGAATGCACTAAAAGTTG
CTCAGGATTTGGGTTACGCAGAGTCTGATCCCAGCGCAGATATAGAGGGGCATGATGCAGCCGCAAAGGCAACAATACTT
GCCTCTTTAGCTTTTCATGCAGATATACCAAGAACTTTGGTGTACCGCGAAGGGATATCTAACATCACCCAGGAAACCAT
AAGCGCAGCAAGGGCGAGTAATAAATTCGTGAGGCTTTTGGCAATATGTGAGCGGATGGCAAATGACACTGATATTAGCG
TCCGTGTGCATCCAGCGCTTATCGATCGCAAGCACCCCCTAGCTGCCGTGCGTGGCAATAAAAACGCAATTTTTGTACAG
GCAAAGCTGGCTGGCCCACTCATGTTTTACGGCTCCGGCGCGGGAGGTCTAGAAACTGCTTCTGCCATCCTTGGTGATGT
AGTGAGTATAGCCCGTAGATACAAGGCTGGATCTATCCCAACCTCCGAGATTTGGATAGATAATAGACGCAAGAGATCTG
TGAAAGTGAGTCCGATAGATCTTATAAAAACCAGATATCAGTTTGTTTTATTTGCTATACAGTCTCCCGAGGCGCTTGCT
CAGATTGCCAGGACATTCGCGGAATGTAAGGTGCTTTTGGATAGCCTGGTCCAGTCCCGGGTGGATTGTTCGAATCTACA
CACAAAACTCTCTGAGTCTGATGTAATAATGCGTCTGGTAGTTGTAACTGAGGTTGTTACAGACTCCGCCATATCCTCTG
TAATTGTGGAGTTAAAAAATTGCTCGCATGTTGTGAGTATAGTAAGTGTTATGCGGATTGAACAGGGCGGTTTTGAAGAA
ACTGGCCTTGCGTTACCGGAAACGGCGTGA

Upstream 100 bases:

>100_bases
TCGCTGATGCCAGAACTACCAGTTTTTCTGCCACTGTGGTTGATAGAGTGCGCAATTTTCAAAATTTCTTTTAGCTCTTG
AGTTTCTTGATTCTTAGACC

Downstream 100 bases:

>100_bases
TGCACGATGTATTTTAGAGGGATTATCAACGAGTACAGATCACGCCTGGGGCTTGACGCGGACCTTAAGGTTGTTACCCT
TGGTGAGGGTAATACCCCCC

Product: homoserine dehydrogenase

Products: NA

Alternate protein names: HDH [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MPLRNSKISGLITKQSSLGSVYILYNRVLGACVDYRSLKLALFGAGRVGSCVARFLLSNADELKIRTGGLDIELIGIGVK
DLSKSRELDASYYTDDLEGLILRADVVIELIGGLHPAKELITRALQVGADVVSANKTLISEHGRELLALASSVGAQFLYE
AAVCAAIPVIRPLKDSLSGDVIQKISGIVNGTTNFILDRMESTGDSLENALKVAQDLGYAESDPSADIEGHDAAAKATIL
ASLAFHADIPRTLVYREGISNITQETISAARASNKFVRLLAICERMANDTDISVRVHPALIDRKHPLAAVRGNKNAIFVQ
AKLAGPLMFYGSGAGGLETASAILGDVVSIARRYKAGSIPTSEIWIDNRRKRSVKVSPIDLIKTRYQFVLFAIQSPEALA
QIARTFAECKVLLDSLVQSRVDCSNLHTKLSESDVIMRLVVVTEVVTDSAISSVIVELKNCSHVVSIVSVMRIEQGGFEE
TGLALPETA

Sequences:

>Translated_489_residues
MPLRNSKISGLITKQSSLGSVYILYNRVLGACVDYRSLKLALFGAGRVGSCVARFLLSNADELKIRTGGLDIELIGIGVK
DLSKSRELDASYYTDDLEGLILRADVVIELIGGLHPAKELITRALQVGADVVSANKTLISEHGRELLALASSVGAQFLYE
AAVCAAIPVIRPLKDSLSGDVIQKISGIVNGTTNFILDRMESTGDSLENALKVAQDLGYAESDPSADIEGHDAAAKATIL
ASLAFHADIPRTLVYREGISNITQETISAARASNKFVRLLAICERMANDTDISVRVHPALIDRKHPLAAVRGNKNAIFVQ
AKLAGPLMFYGSGAGGLETASAILGDVVSIARRYKAGSIPTSEIWIDNRRKRSVKVSPIDLIKTRYQFVLFAIQSPEALA
QIARTFAECKVLLDSLVQSRVDCSNLHTKLSESDVIMRLVVVTEVVTDSAISSVIVELKNCSHVVSIVSVMRIEQGGFEE
TGLALPETA
>Mature_488_residues
PLRNSKISGLITKQSSLGSVYILYNRVLGACVDYRSLKLALFGAGRVGSCVARFLLSNADELKIRTGGLDIELIGIGVKD
LSKSRELDASYYTDDLEGLILRADVVIELIGGLHPAKELITRALQVGADVVSANKTLISEHGRELLALASSVGAQFLYEA
AVCAAIPVIRPLKDSLSGDVIQKISGIVNGTTNFILDRMESTGDSLENALKVAQDLGYAESDPSADIEGHDAAAKATILA
SLAFHADIPRTLVYREGISNITQETISAARASNKFVRLLAICERMANDTDISVRVHPALIDRKHPLAAVRGNKNAIFVQA
KLAGPLMFYGSGAGGLETASAILGDVVSIARRYKAGSIPTSEIWIDNRRKRSVKVSPIDLIKTRYQFVLFAIQSPEALAQ
IARTFAECKVLLDSLVQSRVDCSNLHTKLSESDVIMRLVVVTEVVTDSAISSVIVELKNCSHVVSIVSVMRIEQGGFEET
GLALPETA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=250, Percent_Identity=29.2, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790376, Length=359, Percent_Identity=27.2980501392758, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322599, Length=263, Percent_Identity=29.277566539924, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR005106
- InterPro:   IPR016204
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =1.1.1.3 [H]

Molecular weight: Translated: 52515; Mature: 52384

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01042 HOMOSER_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRNSKISGLITKQSSLGSVYILYNRVLGACVDYRSLKLALFGAGRVGSCVARFLLSNA
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCC
DELKIRTGGLDIELIGIGVKDLSKSRELDASYYTDDLEGLILRADVVIELIGGLHPAKEL
CCEEEEECCEEEEEEECCHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHCCCHHHHH
ITRALQVGADVVSANKTLISEHGRELLALASSVGAQFLYEAAVCAAIPVIRPLKDSLSGD
HHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VIQKISGIVNGTTNFILDRMESTGDSLENALKVAQDLGYAESDPSADIEGHDAAAKATIL
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
ASLAFHADIPRTLVYREGISNITQETISAARASNKFVRLLAICERMANDTDISVRVHPAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
IDRKHPLAAVRGNKNAIFVQAKLAGPLMFYGSGAGGLETASAILGDVVSIARRYKAGSIP
HCCCCCCEEEECCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TSEIWIDNRRKRSVKVSPIDLIKTRYQFVLFAIQSPEALAQIARTFAECKVLLDSLVQSR
CHHEEECCCCCCCEECCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
VDCSNLHTKLSESDVIMRLVVVTEVVTDSAISSVIVELKNCSHVVSIVSVMRIEQGGFEE
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TGLALPETA
CCCCCCCCC
>Mature Secondary Structure 
PLRNSKISGLITKQSSLGSVYILYNRVLGACVDYRSLKLALFGAGRVGSCVARFLLSNA
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCC
DELKIRTGGLDIELIGIGVKDLSKSRELDASYYTDDLEGLILRADVVIELIGGLHPAKEL
CCEEEEECCEEEEEEECCHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHCCCHHHHH
ITRALQVGADVVSANKTLISEHGRELLALASSVGAQFLYEAAVCAAIPVIRPLKDSLSGD
HHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VIQKISGIVNGTTNFILDRMESTGDSLENALKVAQDLGYAESDPSADIEGHDAAAKATIL
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
ASLAFHADIPRTLVYREGISNITQETISAARASNKFVRLLAICERMANDTDISVRVHPAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
IDRKHPLAAVRGNKNAIFVQAKLAGPLMFYGSGAGGLETASAILGDVVSIARRYKAGSIP
HCCCCCCEEEECCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TSEIWIDNRRKRSVKVSPIDLIKTRYQFVLFAIQSPEALAQIARTFAECKVLLDSLVQSR
CHHEEECCCCCCCEECCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
VDCSNLHTKLSESDVIMRLVVVTEVVTDSAISSVIVELKNCSHVVSIVSVMRIEQGGFEE
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TGLALPETA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2835591; 3320973; 9362124; 12948626; 1902466 [H]