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The map label for this gene is xthA1 [H]

Identifier: 28572478

GI number: 28572478

Start: 355281

End: 356120

Strand: Direct

Name: xthA1 [H]

Synonym: TW323

Alternate gene names: 28572478

Gene position: 355281-356120 (Clockwise)

Preceding gene: 28572477

Following gene: 28572479

Centisome position: 38.37

GC content: 49.64

Gene sequence:

>840_bases
GTGCGCGTCGGTACATGGAATGTCAATTCAATTAGGGCACGTCTTGAACGTGTTTTGGCTTGGCTTAAAAATGAATGCAT
TGATGTTGCTGCCCTACAGGAGACTAAGGTCAGAACTGGGGATTTTCCTGTCGATGCCTTTAGAGACGCGGGATATTATT
CTGAAGCTCATGGCCTTAGGGGATTTGAGGGCGTTGCAATTGTTAGCAGGTTCCCGATTAGAAGGGTTACGGTTAACATT
CCTCGTGCACCCGGATTCTTGAGTGACCGCTGGGGTACGGATATTCCGCAGGAGGCTCGGGCAATTTCCGCAACGACTGG
TGGTATTCGTTTGTATAGCCTGTACGTTCCCAACGGAAGGGAAGTCGGCTCCCCGCACTTTGAGTATAAGCTCGCATGGC
TTAAAGCTCTTGCATCTCATGTCAGGACTGTTCTTAGAAGAACTCCAGGCATTCCTCTAATGCTTGTTGGGGATTTTAAC
ATCGTCACCTGCGAACAAGATACAGCTGATCCCGATTTTCAGCTGCCTAATGACATAACCTGTTCAGGTCCTGAGCGTGA
GGCCTTTCAGGCCATCCTTGACACGGGGATGTTTGACCTTATCCGCCCTTTTGCCCCAATTGGCTTTACATCCTGGAGTT
ATCAGCGTCAGCGTTTTCAGAATAACCAAGGCCTCAGGATAGATTTCATGCTCGGGTCTGATTCAGTCCAAAGAAGGACT
ATTTGGGGTGAAATCGATCGGGTGCAGAGAAGGCATCTTTTTGGCCTTACTCCCAGTGATCACGCCCCACTGGTGTCGGT
TATTGATCCCCCTAGGCGGACATTTCGCAGAGACGACTGA

Upstream 100 bases:

>100_bases
ACGGTCCTTTATATCCGGGCAGAAAATCACTTTCTTTGCAACATCTCGTTTTATCGTGTCCTAAACGCTCACTGCGAACC
ATTTGGTGTATCATCTTTGC

Downstream 100 bases:

>100_bases
ACCTACCTGATTTTTGCAGTGAGAAAATTCCTGTTTCGTCGGACCTCTCCCTTTGCGCTCGGATACGACAAACGGCAAGT
TGACACGTTTTTTTCGGCTG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRVGTWNVNSIRARLERVLAWLKNECIDVAALQETKVRTGDFPVDAFRDAGYYSEAHGLRGFEGVAIVSRFPIRRVTVNI
PRAPGFLSDRWGTDIPQEARAISATTGGIRLYSLYVPNGREVGSPHFEYKLAWLKALASHVRTVLRRTPGIPLMLVGDFN
IVTCEQDTADPDFQLPNDITCSGPEREAFQAILDTGMFDLIRPFAPIGFTSWSYQRQRFQNNQGLRIDFMLGSDSVQRRT
IWGEIDRVQRRHLFGLTPSDHAPLVSVIDPPRRTFRRDD

Sequences:

>Translated_279_residues
MRVGTWNVNSIRARLERVLAWLKNECIDVAALQETKVRTGDFPVDAFRDAGYYSEAHGLRGFEGVAIVSRFPIRRVTVNI
PRAPGFLSDRWGTDIPQEARAISATTGGIRLYSLYVPNGREVGSPHFEYKLAWLKALASHVRTVLRRTPGIPLMLVGDFN
IVTCEQDTADPDFQLPNDITCSGPEREAFQAILDTGMFDLIRPFAPIGFTSWSYQRQRFQNNQGLRIDFMLGSDSVQRRT
IWGEIDRVQRRHLFGLTPSDHAPLVSVIDPPRRTFRRDD
>Mature_279_residues
MRVGTWNVNSIRARLERVLAWLKNECIDVAALQETKVRTGDFPVDAFRDAGYYSEAHGLRGFEGVAIVSRFPIRRVTVNI
PRAPGFLSDRWGTDIPQEARAISATTGGIRLYSLYVPNGREVGSPHFEYKLAWLKALASHVRTVLRRTPGIPLMLVGDFN
IVTCEQDTADPDFQLPNDITCSGPEREAFQAILDTGMFDLIRPFAPIGFTSWSYQRQRFQNNQGLRIDFMLGSDSVQRRT
IWGEIDRVQRRHLFGLTPSDHAPLVSVIDPPRRTFRRDD

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=28.7272727272727, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=28.7272727272727, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=28.7272727272727, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788046, Length=290, Percent_Identity=28.2758620689655, Blast_Score=88, Evalue=6e-19,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=26.7399267399267, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136678, Length=273, Percent_Identity=26.7399267399267, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31689; Mature: 31689

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGTWNVNSIRARLERVLAWLKNECIDVAALQETKVRTGDFPVDAFRDAGYYSEAHGLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHCCCC
GFEGVAIVSRFPIRRVTVNIPRAPGFLSDRWGTDIPQEARAISATTGGIRLYSLYVPNGR
CCCCCHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCHHHHEEEECCCCEEEEEEECCCCC
EVGSPHFEYKLAWLKALASHVRTVLRRTPGIPLMLVGDFNIVTCEQDTADPDFQLPNDIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCC
CSGPEREAFQAILDTGMFDLIRPFAPIGFTSWSYQRQRFQNNQGLRIDFMLGSDSVQRRT
CCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHH
IWGEIDRVQRRHLFGLTPSDHAPLVSVIDPPRRTFRRDD
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHCCCCC
>Mature Secondary Structure
MRVGTWNVNSIRARLERVLAWLKNECIDVAALQETKVRTGDFPVDAFRDAGYYSEAHGLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHCCCC
GFEGVAIVSRFPIRRVTVNIPRAPGFLSDRWGTDIPQEARAISATTGGIRLYSLYVPNGR
CCCCCHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCHHHHEEEECCCCEEEEEEECCCCC
EVGSPHFEYKLAWLKALASHVRTVLRRTPGIPLMLVGDFNIVTCEQDTADPDFQLPNDIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCC
CSGPEREAFQAILDTGMFDLIRPFAPIGFTSWSYQRQRFQNNQGLRIDFMLGSDSVQRRT
CCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHH
IWGEIDRVQRRHLFGLTPSDHAPLVSVIDPPRRTFRRDD
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]