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The map label for this gene is tsf

Identifier: 28572474

GI number: 28572474

Start: 352019

End: 352840

Strand: Direct

Name: tsf

Synonym: TW319

Alternate gene names: 28572474

Gene position: 352019-352840 (Clockwise)

Preceding gene: 161501891

Following gene: 28572475

Centisome position: 38.02

GC content: 50.49

Gene sequence:

>822_bases
GTGGTGGATATAAGCATGGCTGATGTGAGGCTGTTGCGTGAGCGTCTCGGTGTTGGCGTTATGGATAGCAGGAACGCGCT
CATAGAGGCCGGTGGCGATATCGAAAGGGCGGTAGAGATTTTAAGGCTTAAAGGCCTTAAATCGGCTGAAAAGCGCGAGG
GGCGCTCTGTTAGCGAGGGTCTTGTGGTCTCAAGGCAGTTTGCGACTCATGCCGTTTTGGCTGAGTTGTGCTGTGAAACG
GATTTTGTTGCTAAAAGTGACAGGTTTCTGGCCCTGTCGGAGAGAGTTGCTGATCTTGTCAGCGATGCCGATTCTCTTGA
AACGGCCCTGCGTGTCCGGTGCGATGAAGGGTCTGTTGCTGATCTAATTGCCCTAGAAGCCGCTGTTCTGGGCGAGAATG
TAGCCTTGCGCCGGTTTGCCCGTGTTGAAGGCAGCAGATTTTCTGTTTATATGCACCGCACAAGCTCCGATCTTCCTCCG
CAGGTTGGGGTCATTCTTGCGTATGAAGGGCATGATGACGCAACAGCCCGCTTCATCGCCCAGCATATCGCGTTTGCGGC
TCCCGAATATCTATCGGTTGGTGATATACCTCAGGGTATTCTCCAGAGGGAACGTGATTTGCTCACTGAAATATCAAGGG
GTGAAGGTAAGCCGGAAGAGGTTTTACCTCAGATTGTTGAGGGTCGTTTAGTCAAGCTTTATAAGCAGAATGTGCTTCTT
GAACAGGATTATGTGCGTGACAATAAGGTAACTATTTCAAAAGTGCTAGAAGCTACAGGTCTTCGTGTGATATCTTTTGC
GCGCTTTCGTGTTGGAACCTGA

Upstream 100 bases:

>100_bases
CACAGATCGCCCTCTTGATGGTGATCTTTCCAAGGGGCCTGCCCCGCAGGACGAAGAGCTGTCAGATTAGGATATCTTTC
TTTATGAAGGAAGTGAGTGT

Downstream 100 bases:

>100_bases
GTGTGCATAGATGGCCAGTCGCAGAGTTCTTCTGAAGCTTTCGGGTGAATCCTTTGGTGGCGGCGCTCCGTTGGTTGACC
CTGACGTGGTTAGCGCAATT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MVDISMADVRLLRERLGVGVMDSRNALIEAGGDIERAVEILRLKGLKSAEKREGRSVSEGLVVSRQFATHAVLAELCCET
DFVAKSDRFLALSERVADLVSDADSLETALRVRCDEGSVADLIALEAAVLGENVALRRFARVEGSRFSVYMHRTSSDLPP
QVGVILAYEGHDDATARFIAQHIAFAAPEYLSVGDIPQGILQRERDLLTEISRGEGKPEEVLPQIVEGRLVKLYKQNVLL
EQDYVRDNKVTISKVLEATGLRVISFARFRVGT

Sequences:

>Translated_273_residues
MVDISMADVRLLRERLGVGVMDSRNALIEAGGDIERAVEILRLKGLKSAEKREGRSVSEGLVVSRQFATHAVLAELCCET
DFVAKSDRFLALSERVADLVSDADSLETALRVRCDEGSVADLIALEAAVLGENVALRRFARVEGSRFSVYMHRTSSDLPP
QVGVILAYEGHDDATARFIAQHIAFAAPEYLSVGDIPQGILQRERDLLTEISRGEGKPEEVLPQIVEGRLVKLYKQNVLL
EQDYVRDNKVTISKVLEATGLRVISFARFRVGT
>Mature_273_residues
MVDISMADVRLLRERLGVGVMDSRNALIEAGGDIERAVEILRLKGLKSAEKREGRSVSEGLVVSRQFATHAVLAELCCET
DFVAKSDRFLALSERVADLVSDADSLETALRVRCDEGSVADLIALEAAVLGENVALRRFARVEGSRFSVYMHRTSSDLPP
QVGVILAYEGHDDATARFIAQHIAFAAPEYLSVGDIPQGILQRERDLLTEISRGEGKPEEVLPQIVEGRLVKLYKQNVLL
EQDYVRDNKVTISKVLEATGLRVISFARFRVGT

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=295, Percent_Identity=28.135593220339, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786366, Length=277, Percent_Identity=32.129963898917, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_TROW8 (Q83NN3)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789254.1
- ProteinModelPortal:   Q83NN3
- SMR:   Q83NN3
- STRING:   Q83NN3
- GeneID:   1064607
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW319
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   Q83NN3
- ProtClustDB:   PRK09377
- BioCyc:   TWHI218496:TW0299-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30094; Mature: 30094

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDISMADVRLLRERLGVGVMDSRNALIEAGGDIERAVEILRLKGLKSAEKREGRSVSEG
CCCCCHHHHHHHHHHHCCCCCCCCCHHEECCCCHHHHHHHHHHHCCCCHHHHCCCCHHHH
LVVSRQFATHAVLAELCCETDFVAKSDRFLALSERVADLVSDADSLETALRVRCDEGSVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHEECCCCCHH
DLIALEAAVLGENVALRRFARVEGSRFSVYMHRTSSDLPPQVGVILAYEGHDDATARFIA
HHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHH
QHIAFAAPEYLSVGDIPQGILQRERDLLTEISRGEGKPEEVLPQIVEGRLVKLYKQNVLL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQDYVRDNKVTISKVLEATGLRVISFARFRVGT
HHHHCCCCCHHHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure
MVDISMADVRLLRERLGVGVMDSRNALIEAGGDIERAVEILRLKGLKSAEKREGRSVSEG
CCCCCHHHHHHHHHHHCCCCCCCCCHHEECCCCHHHHHHHHHHHCCCCHHHHCCCCHHHH
LVVSRQFATHAVLAELCCETDFVAKSDRFLALSERVADLVSDADSLETALRVRCDEGSVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHEECCCCCHH
DLIALEAAVLGENVALRRFARVEGSRFSVYMHRTSSDLPPQVGVILAYEGHDDATARFIA
HHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHH
QHIAFAAPEYLSVGDIPQGILQRERDLLTEISRGEGKPEEVLPQIVEGRLVKLYKQNVLL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQDYVRDNKVTISKVLEATGLRVISFARFRVGT
HHHHCCCCCHHHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174