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The map label for this gene is pyrH

Identifier: 28572475

GI number: 28572475

Start: 352851

End: 353546

Strand: Direct

Name: pyrH

Synonym: TW320

Alternate gene names: 28572475

Gene position: 352851-353546 (Clockwise)

Preceding gene: 28572474

Following gene: 28572476

Centisome position: 38.11

GC content: 49.28

Gene sequence:

>696_bases
ATGGCCAGTCGCAGAGTTCTTCTGAAGCTTTCGGGTGAATCCTTTGGTGGCGGCGCTCCGTTGGTTGACCCTGACGTGGT
TAGCGCAATTGCCAGCGAAATAGTGAGGGTGTCAAAAACAATACAAATTGCCATTGTGGTTGGCGGTGGTAACTATTTCA
GGGGGGCAGAATTAAGTCGTCGGGGCATGGCAAGGGATCGCGCAGATTACATGGGCATGCTCGGGACTGTGATTAACGCC
TTGGCACTTCAGGACTTTCTGGAACAGGCCGGTGGCGATACTAGGGTCCAGTCGGCAATTAGCATGTCTCAAGTTGCTGA
GCTATATGTGCCCCGTCGGGCCGAGAGACATCTTACGAAGGGCAGGGTTGTTATATTCGCTGCCGGTGCGGGTATGCCAT
ATTTTTCAACCGATACTGTTGCAGTTCAGAGAGCGCTTGAAATCAAGGCAGATATTGTTCTGATTGCTAAAAATGGTGTA
GATGGCGTTTATACAGATGATCCGCAAATAAATCCAAATGCAAAGAAAATATACAAAATTACGTATCGCGAAGCGCTGGA
GAGGGGTTTGCGTGTTGTTGATTCCGCTGCCCTTGGGCTTTGTATGGAGCACGGATTGCCAATGCGTGTATTTGGGATGG
AAAGTCTTAGAGATGCTGTTTCCGGTCTTAAAGTCGGCACGGAGCTAAGCCAATAA

Upstream 100 bases:

>100_bases
GTGCGTGACAATAAGGTAACTATTTCAAAAGTGCTAGAAGCTACAGGTCTTCGTGTGATATCTTTTGCGCGCTTTCGTGT
TGGAACCTGAGTGTGCATAG

Downstream 100 bases:

>100_bases
AGTTGTGGTGTGGTTAGGACTTTTCCTGTTAGATCCTTCGGCTAAACAGATTCCGCAACTCTGGGGTGTGATTTTGAGAT
AAGTGGGTTGTAAGTTTTGT

Product: uridylate kinase

Products: NA

Alternate protein names: UK; Uridine monophosphate kinase; UMP kinase; UMPK

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MASRRVLLKLSGESFGGGAPLVDPDVVSAIASEIVRVSKTIQIAIVVGGGNYFRGAELSRRGMARDRADYMGMLGTVINA
LALQDFLEQAGGDTRVQSAISMSQVAELYVPRRAERHLTKGRVVIFAAGAGMPYFSTDTVAVQRALEIKADIVLIAKNGV
DGVYTDDPQINPNAKKIYKITYREALERGLRVVDSAALGLCMEHGLPMRVFGMESLRDAVSGLKVGTELSQ

Sequences:

>Translated_231_residues
MASRRVLLKLSGESFGGGAPLVDPDVVSAIASEIVRVSKTIQIAIVVGGGNYFRGAELSRRGMARDRADYMGMLGTVINA
LALQDFLEQAGGDTRVQSAISMSQVAELYVPRRAERHLTKGRVVIFAAGAGMPYFSTDTVAVQRALEIKADIVLIAKNGV
DGVYTDDPQINPNAKKIYKITYREALERGLRVVDSAALGLCMEHGLPMRVFGMESLRDAVSGLKVGTELSQ
>Mature_230_residues
ASRRVLLKLSGESFGGGAPLVDPDVVSAIASEIVRVSKTIQIAIVVGGGNYFRGAELSRRGMARDRADYMGMLGTVINAL
ALQDFLEQAGGDTRVQSAISMSQVAELYVPRRAERHLTKGRVVIFAAGAGMPYFSTDTVAVQRALEIKADIVLIAKNGVD
GVYTDDPQINPNAKKIYKITYREALERGLRVVDSAALGLCMEHGLPMRVFGMESLRDAVSGLKVGTELSQ

Specific function: Catalyzes the reversible phosphorylation of UMP to UDP

COG id: COG0528

COG function: function code F; Uridylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UMP kinase family

Homologues:

Organism=Escherichia coli, GI1786367, Length=233, Percent_Identity=44.6351931330472, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRH_TROW8 (Q83HZ6)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789255.1
- HSSP:   P65938
- ProteinModelPortal:   Q83HZ6
- SMR:   Q83HZ6
- STRING:   Q83HZ6
- GeneID:   1064608
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW320
- eggNOG:   COG0528
- HOGENOM:   HBG497552
- OMA:   RHMEKGR
- ProtClustDB:   PRK00358
- BioCyc:   TWHI218496:TW0300-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01220_B
- InterPro:   IPR001048
- InterPro:   IPR011817
- InterPro:   IPR015963
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF005650
- TIGRFAMs:   TIGR02075

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.4.22

Molecular weight: Translated: 24817; Mature: 24686

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: BINDING 49-49 BINDING 50-50 BINDING 54-54 BINDING 69-69 BINDING 158-158 BINDING 164-164 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRRVLLKLSGESFGGGAPLVDPDVVSAIASEIVRVSKTIQIAIVVGGGNYFRGAELSR
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCHHHH
RGMARDRADYMGMLGTVINALALQDFLEQAGGDTRVQSAISMSQVAELYVPRRAERHLTK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCC
GRVVIFAAGAGMPYFSTDTVAVQRALEIKADIVLIAKNGVDGVYTDDPQINPNAKKIYKI
CCEEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCEECCCCCCCCCHHHHEEH
TYREALERGLRVVDSAALGLCMEHGLPMRVFGMESLRDAVSGLKVGTELSQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASRRVLLKLSGESFGGGAPLVDPDVVSAIASEIVRVSKTIQIAIVVGGGNYFRGAELSR
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCHHHH
RGMARDRADYMGMLGTVINALALQDFLEQAGGDTRVQSAISMSQVAELYVPRRAERHLTK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCC
GRVVIFAAGAGMPYFSTDTVAVQRALEIKADIVLIAKNGVDGVYTDDPQINPNAKKIYKI
CCEEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCEECCCCCCCCCHHHHEEH
TYREALERGLRVVDSAALGLCMEHGLPMRVFGMESLRDAVSGLKVGTELSQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174