Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 28572458

GI number: 28572458

Start: 339304

End: 339981

Strand: Reverse

Name: lipB [H]

Synonym: TW302

Alternate gene names: 28572458

Gene position: 339981-339304 (Counterclockwise)

Preceding gene: 28572472

Following gene: 28572457

Centisome position: 36.72

GC content: 46.02

Gene sequence:

>678_bases
GTGTACTCTATTCGAGTGCTAGCAATTGAGTGCATCAGACTTGCACCTGAGTATCTCCCCTTTGAGGAAGGATGGAGAAT
GCAACAAGAACTTCACAAAAAGATAACCGAAAGTCAAACAAATAAAAACCGTCAGGCATCAATGATTTTTTGTGAACACG
AGCCCGTTTATACAGCGGGAGCCAGAACCAGGAGCTGGGAGATGCCACAGAACGAAAAGGTGATTAGAGTTGGTCGAGGT
GGCAAAATAACCTGGCATGGCCCCGGGCAATTAGTTGGGTATCCGATACTTAGTCTGGGCGCAACCCCAGATGTTTTGCG
CTACGTAAGACAAATCGAAGAAGGGCTCATAAACGCACTGCAGTCAGTAGGAATAAATGGGTTCCGCATCGCCGGCAGGA
GCGGGGTTTGGGTAAGAAATGGGGTTAGCGATGAAAAAGTGGCGGCCATAGGTGTTAGGGTTCAAAAAAACGTCACTCTT
CACGGTTTTGCATTAAATTGCAGTAATGACCTTGCGCCATTTGAAAAAATAGTGCCGTGCGGAATAAGCGACGCCGGAGT
AACAACGATCTCAAGAATTTTGAAGAGAACTATTACACCAAAAGAAATACTTGACAGCGTGTTCAACAGTATCTGCAGCG
CGCTTGAATATATAAATACCTGCCGTGGCAACGTATAG

Upstream 100 bases:

>100_bases
CCCGATTAGTCCGGTTTTAAGCGAAATCCTGACTTAGACAGTCAGAACAGAGCGGACGTATCCCAACGGGGTGCACTCGA
ATTTACCCGGATACTGGTTG

Downstream 100 bases:

>100_bases
ACATGCAGCGTGCAAAATATGAACGATTCTGGCAATTCAAGCAAAGTTAATGTAAGGCCCCCTTCAGCCGGTTTGGGAGC
ACCCTCCCCGGGCAAGCGTA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MYSIRVLAIECIRLAPEYLPFEEGWRMQQELHKKITESQTNKNRQASMIFCEHEPVYTAGARTRSWEMPQNEKVIRVGRG
GKITWHGPGQLVGYPILSLGATPDVLRYVRQIEEGLINALQSVGINGFRIAGRSGVWVRNGVSDEKVAAIGVRVQKNVTL
HGFALNCSNDLAPFEKIVPCGISDAGVTTISRILKRTITPKEILDSVFNSICSALEYINTCRGNV

Sequences:

>Translated_225_residues
MYSIRVLAIECIRLAPEYLPFEEGWRMQQELHKKITESQTNKNRQASMIFCEHEPVYTAGARTRSWEMPQNEKVIRVGRG
GKITWHGPGQLVGYPILSLGATPDVLRYVRQIEEGLINALQSVGINGFRIAGRSGVWVRNGVSDEKVAAIGVRVQKNVTL
HGFALNCSNDLAPFEKIVPCGISDAGVTTISRILKRTITPKEILDSVFNSICSALEYINTCRGNV
>Mature_225_residues
MYSIRVLAIECIRLAPEYLPFEEGWRMQQELHKKITESQTNKNRQASMIFCEHEPVYTAGARTRSWEMPQNEKVIRVGRG
GKITWHGPGQLVGYPILSLGATPDVLRYVRQIEEGLINALQSVGINGFRIAGRSGVWVRNGVSDEKVAAIGVRVQKNVTL
HGFALNCSNDLAPFEKIVPCGISDAGVTTISRILKRTITPKEILDSVFNSICSALEYINTCRGNV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=217, Percent_Identity=34.1013824884793, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081767, Length=146, Percent_Identity=35.6164383561644, Blast_Score=94, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323268, Length=167, Percent_Identity=31.1377245508982, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355483, Length=204, Percent_Identity=36.7647058823529, Blast_Score=137, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25028; Mature: 25028

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSIRVLAIECIRLAPEYLPFEEGWRMQQELHKKITESQTNKNRQASMIFCEHEPVYTAG
CCEEHHHHHHHHHHCHHHCCHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECC
ARTRSWEMPQNEKVIRVGRGGKITWHGPGQLVGYPILSLGATPDVLRYVRQIEEGLINAL
CCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
QSVGINGFRIAGRSGVWVRNGVSDEKVAAIGVRVQKNVTLHGFALNCSNDLAPFEKIVPC
HHCCCCCEEEECCCCCEEECCCCCCCEEEEEEEEECCEEEEEEEEECCCCCCHHHHHCCC
GISDAGVTTISRILKRTITPKEILDSVFNSICSALEYINTCRGNV
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYSIRVLAIECIRLAPEYLPFEEGWRMQQELHKKITESQTNKNRQASMIFCEHEPVYTAG
CCEEHHHHHHHHHHCHHHCCHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECC
ARTRSWEMPQNEKVIRVGRGGKITWHGPGQLVGYPILSLGATPDVLRYVRQIEEGLINAL
CCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
QSVGINGFRIAGRSGVWVRNGVSDEKVAAIGVRVQKNVTLHGFALNCSNDLAPFEKIVPC
HHCCCCCEEEECCCCCEEECCCCCCCEEEEEEEEECCEEEEEEEEECCCCCCHHHHHCCC
GISDAGVTTISRILKRTITPKEILDSVFNSICSALEYINTCRGNV
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]