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The map label for this gene is lipA

Identifier: 28572457

GI number: 28572457

Start: 338230

End: 339285

Strand: Reverse

Name: lipA

Synonym: TW301

Alternate gene names: 28572457

Gene position: 339285-338230 (Counterclockwise)

Preceding gene: 28572458

Following gene: 28572456

Centisome position: 36.64

GC content: 48.11

Gene sequence:

>1056_bases
ATGAACGATTCTGGCAATTCAAGCAAAGTTAATGTAAGGCCCCCTTCAGCCGGTTTGGGAGCACCCTCCCCGGGCAAGCG
TAAGATGCTCCGCCTGGAGGTACGAAATTCGCAGGTTCCTATAGAACGGAAGCCCTCATGGATAAGGGCTCGTGCAACTA
TTGGTACAGAGTACAGAAAGGTTCAGGAACGTGTTAAGAAACAGAATTTGCGGACTGTTTGTCAAGAAGCCGGATGTCCG
AATATCTACGAATGCTGGGAGGATAGAGAGGCAACTTTCCTGATCGGAGGATCGCAGTGTACTCGCAGGTGTGATTTTTG
TCAGATAGACACGGGAAAACCTGCAGAGCTCGACCTGGATGAGCCAAAACGGGTTGGACAGTCCGTGGCGCAAATGAAGT
TACGCTATGCCACCGTAACCGGAGTAGCACGCGACGACCTGCCCGATGGGGGTGTCTGGCTCTATGCAGAAACAATCCGA
GAAATACACAAACAGTGCCCTGGAAGTGGGGTTGAAATACTCATACCAGATTTTAACGGTAAGCCAGAGTTGCTCCAGCA
AATCTTCGAAGCACAACCGGAAGTGTACGCACACAATATAGAAACTGTTCCACGGATATTCAGGCGCATTAGACCGGCAT
TCAGATACGACAGATCGCTGGATGTTATATCACAAGGTCAAAAAGCTGGGATGATAACAAAATCAAATCTTATTCTTGGA
ATGGGCGAGACCGGTGAAGAGGTAACACAGGCATTGAGAGACCTCAAAAGTGCCGGTTGTGACATTGTAACAATAACTCA
GTATCTAAGACCATCGCCCAGGCATTTACCCGTGGCAAGATGGGTAAAACCACAAGAGTTCATAGAATATAAAGAGCAAG
CCAAAGAGATCGGATTCTCAGGCGTTTTGGCCGGACCTTTAGTTAGATCATCATACCGTGCCGGAAAACTGTGGGCACAA
TCTGTGAAAGCTAAGATGGTTGAAATACCCGAGCGCGTACGGAAACTCATAAACGAAATCGAGATGCAGGAAACGTCCTT
TAGGCAAGCAGTCTAG

Upstream 100 bases:

>100_bases
ACCAAAAGAAATACTTGACAGCGTGTTCAACAGTATCTGCAGCGCGCTTGAATATATAAATACCTGCCGTGGCAACGTAT
AGACATGCAGCGTGCAAAAT

Downstream 100 bases:

>100_bases
TATGTCTTCAGTGGAATTTGCAGTACGCATGAGAGCCTGTGCTTTTAGGGTAAAGGTTTATTCTTCGCAAACACATATCA
TTAGGGTAAAAATCTTTCGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNDSGNSSKVNVRPPSAGLGAPSPGKRKMLRLEVRNSQVPIERKPSWIRARATIGTEYRKVQERVKKQNLRTVCQEAGCP
NIYECWEDREATFLIGGSQCTRRCDFCQIDTGKPAELDLDEPKRVGQSVAQMKLRYATVTGVARDDLPDGGVWLYAETIR
EIHKQCPGSGVEILIPDFNGKPELLQQIFEAQPEVYAHNIETVPRIFRRIRPAFRYDRSLDVISQGQKAGMITKSNLILG
MGETGEEVTQALRDLKSAGCDIVTITQYLRPSPRHLPVARWVKPQEFIEYKEQAKEIGFSGVLAGPLVRSSYRAGKLWAQ
SVKAKMVEIPERVRKLINEIEMQETSFRQAV

Sequences:

>Translated_351_residues
MNDSGNSSKVNVRPPSAGLGAPSPGKRKMLRLEVRNSQVPIERKPSWIRARATIGTEYRKVQERVKKQNLRTVCQEAGCP
NIYECWEDREATFLIGGSQCTRRCDFCQIDTGKPAELDLDEPKRVGQSVAQMKLRYATVTGVARDDLPDGGVWLYAETIR
EIHKQCPGSGVEILIPDFNGKPELLQQIFEAQPEVYAHNIETVPRIFRRIRPAFRYDRSLDVISQGQKAGMITKSNLILG
MGETGEEVTQALRDLKSAGCDIVTITQYLRPSPRHLPVARWVKPQEFIEYKEQAKEIGFSGVLAGPLVRSSYRAGKLWAQ
SVKAKMVEIPERVRKLINEIEMQETSFRQAV
>Mature_351_residues
MNDSGNSSKVNVRPPSAGLGAPSPGKRKMLRLEVRNSQVPIERKPSWIRARATIGTEYRKVQERVKKQNLRTVCQEAGCP
NIYECWEDREATFLIGGSQCTRRCDFCQIDTGKPAELDLDEPKRVGQSVAQMKLRYATVTGVARDDLPDGGVWLYAETIR
EIHKQCPGSGVEILIPDFNGKPELLQQIFEAQPEVYAHNIETVPRIFRRIRPAFRYDRSLDVISQGQKAGMITKSNLILG
MGETGEEVTQALRDLKSAGCDIVTITQYLRPSPRHLPVARWVKPQEFIEYKEQAKEIGFSGVLAGPLVRSSYRAGKLWAQ
SVKAKMVEIPERVRKLINEIEMQETSFRQAV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=292, Percent_Identity=37.6712328767123, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=37.9591836734694, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI1786846, Length=305, Percent_Identity=41.3114754098361, Blast_Score=249, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=38.3561643835616, Blast_Score=211, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6324770, Length=326, Percent_Identity=38.3435582822086, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI221513272, Length=290, Percent_Identity=42.0689655172414, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_TROW8 (Q83NN8)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789237.1
- ProteinModelPortal:   Q83NN8
- STRING:   Q83NN8
- GeneID:   1064590
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW301
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   TWHI218496:TW0282-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 39597; Mature: 39597

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSGNSSKVNVRPPSAGLGAPSPGKRKMLRLEVRNSQVPIERKPSWIRARATIGTEYRK
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHH
VQERVKKQNLRTVCQEAGCPNIYECWEDREATFLIGGSQCTRRCDFCQIDTGKPAELDLD
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECHHHHCCCCEEECCCCCCCCCCCC
EPKRVGQSVAQMKLRYATVTGVARDDLPDGGVWLYAETIREIHKQCPGSGVEILIPDFNG
CHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEECCCC
KPELLQQIFEAQPEVYAHNIETVPRIFRRIRPAFRYDRSLDVISQGQKAGMITKSNLILG
CHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCEEEE
MGETGEEVTQALRDLKSAGCDIVTITQYLRPSPRHLPVARWVKPQEFIEYKEQAKEIGFS
CCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCC
GVLAGPLVRSSYRAGKLWAQSVKAKMVEIPERVRKLINEIEMQETSFRQAV
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDSGNSSKVNVRPPSAGLGAPSPGKRKMLRLEVRNSQVPIERKPSWIRARATIGTEYRK
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHH
VQERVKKQNLRTVCQEAGCPNIYECWEDREATFLIGGSQCTRRCDFCQIDTGKPAELDLD
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECHHHHCCCCEEECCCCCCCCCCCC
EPKRVGQSVAQMKLRYATVTGVARDDLPDGGVWLYAETIREIHKQCPGSGVEILIPDFNG
CHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEECCCC
KPELLQQIFEAQPEVYAHNIETVPRIFRRIRPAFRYDRSLDVISQGQKAGMITKSNLILG
CHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCEEEE
MGETGEEVTQALRDLKSAGCDIVTITQYLRPSPRHLPVARWVKPQEFIEYKEQAKEIGFS
CCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCC
GVLAGPLVRSSYRAGKLWAQSVKAKMVEIPERVRKLINEIEMQETSFRQAV
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174