Click here to switch to the map view.

The map label for this gene is rne [H]

Identifier: 28572447

GI number: 28572447

Start: 329749

End: 331398

Strand: Direct

Name: rne [H]

Synonym: TW291

Alternate gene names: 28572447

Gene position: 329749-331398 (Clockwise)

Preceding gene: 28572446

Following gene: 28572448

Centisome position: 35.61

GC content: 48.12

Gene sequence:

>1650_bases
GTGTTTGGTAGATGCATGGTTGATACGGGATCGAAGCGAGCCAGCGGTTATAAAGTTGTGTTTCAAGCTCCTGACTTTTC
AGCGCGTATTGTCTCGCAAAAAAAACGGAGCGATATCACGCCTCTCCGTCAATCAAAACGTAGGGCGTCGAGAACTTCCC
CCGCTGAAACAGGAATTTTACGGCGTAATACGCAGCGTGTTATGTTGGTTCGCGGCTCTGGCGCCTCAGATGTGCAAGTT
GGCATCCTAGAGGAGGGCATCCTTGTTGAGTACTACATGGCTCGTGGTGCTTCAAGTTCTCTTGTAGGGAATATATATAT
GGGCAGAGTGCAGAATGTCTTGCCCGGAATGGAAGCTGCTTTTGTTGATATCGGCTGTGGCCGTAATGCCGTTTTGTATT
CTGGCGAAGCTGTTTGGGAGGGCGTTAAGGCTCAGAGTATCGAAAATACCGTTAGAGAAGGCTCTTCGGTCCTTGTTCAG
GTCACGAAGGATGCGGTTGCTAACAAGGGCCCAAGAGTTACTGGACAACTATCTCTCGCGGGTCGTTACTTGGTTTATGC
CCCGGGTTCGGGCGGTGTAAGTCGTCGTATTGCACCATCCGAGCGCGCGCGCCTTCAGAAATCTCTGAAGGCAGCTGTGG
AAGATGTCTCAAGTCTCATAATACGCACAGCAGCCGTTGGCGCAACAAGTGAACAGCTTGGTAATGATGCAAAGATGCTA
TGCGAGGCCTACAGGGCTATAAAGGAAAAGTCAGAAAACGCCACTGCCCCTTCAATAATCTATTCAGAACCCGATCTCCT
AATCAGGCTGCTTAGAGATACGTTCAACAACGACTTTAAAGAATTAATTGTTGAAGGTGCTGAACTCTATGAGCGGGTAA
ATTCCTATTTACAAGACGTATCACCGGAAATGCTGGGTAAAGTAAGTAAAAGTTCCGAAGAAGACTTGTTTGATGCATAT
CGCATAACGGAACAAATAGATAGATCGTTGAACAGAAAGGTGCTTCTTCCATCTGGCGGGTCCCTCGTAGTTGATCATAC
CGAGGCAATGACTGTTATAGACGTTAACACCGGTAAATTTGTCGGAACGAGTAGCAGCCTAGAGCAAACCGTTACCGACA
ACAATCTAGAAGCGGTTGATGAAATAGTGCGACAGATTAGACTCAGGGACCTCGGTGGAATAGTTGTTATTGACTTTATT
GACATGCTTTTACAAGAGAATCGCGATCTTGTTTATAACAGGCTATTGGAGGCTTTATCTCTTGATAGATCTCGTCACAA
AGTTGCCGAGATAACGAGTTTGGGTCTTGTTCAAATGACCCGCAAAAAACTCGGTGTTGCCTTAGAGCCTGTCAGTGAGT
TGTGTGATCGCTGCTCCGGCCGTGGTAGGGTCATAAATCCAGTACCCAATAAGCCACGTGCGCGGAAAAAGCATAGACTA
AGGCAGGTCAACAAGGAGGCTTCTGATGCTGCGGTGAGAATTATTTCTGCAATGCAAGACACAGCGGGTTATTCTGATGC
ACGCAGTGACACCCTGTCTTCACGCGGCGCCCTTGCGCCAGACAGTCAGGTCTCCATTTCGGTAGGTAATCGTCGTCGTA
GGCTTGTTACATCGACTATTGACGCTAGTGGCGACCCGAGGGCGTCTTAG

Upstream 100 bases:

>100_bases
TCCGTTAACGGACGCTCTTAGCTTTGCGTCGTTTGGTGCCTTTTGCGCATGGGTATCGGTACTGTTTGATATTTCCTGAG
GTCAAGTCTGGCCTCGGATT

Downstream 100 bases:

>100_bases
ATTAAATTTTCTGCGTGCCTTATACGGGTGTTTTATGGCCTATCGTGTCTTTGGGAATTGTTGCCTTGTTGTTTTTTCGT
GTAGAATCTTGTGTCGTTTC

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MFGRCMVDTGSKRASGYKVVFQAPDFSARIVSQKKRSDITPLRQSKRRASRTSPAETGILRRNTQRVMLVRGSGASDVQV
GILEEGILVEYYMARGASSSLVGNIYMGRVQNVLPGMEAAFVDIGCGRNAVLYSGEAVWEGVKAQSIENTVREGSSVLVQ
VTKDAVANKGPRVTGQLSLAGRYLVYAPGSGGVSRRIAPSERARLQKSLKAAVEDVSSLIIRTAAVGATSEQLGNDAKML
CEAYRAIKEKSENATAPSIIYSEPDLLIRLLRDTFNNDFKELIVEGAELYERVNSYLQDVSPEMLGKVSKSSEEDLFDAY
RITEQIDRSLNRKVLLPSGGSLVVDHTEAMTVIDVNTGKFVGTSSSLEQTVTDNNLEAVDEIVRQIRLRDLGGIVVIDFI
DMLLQENRDLVYNRLLEALSLDRSRHKVAEITSLGLVQMTRKKLGVALEPVSELCDRCSGRGRVINPVPNKPRARKKHRL
RQVNKEASDAAVRIISAMQDTAGYSDARSDTLSSRGALAPDSQVSISVGNRRRRLVTSTIDASGDPRAS

Sequences:

>Translated_549_residues
MFGRCMVDTGSKRASGYKVVFQAPDFSARIVSQKKRSDITPLRQSKRRASRTSPAETGILRRNTQRVMLVRGSGASDVQV
GILEEGILVEYYMARGASSSLVGNIYMGRVQNVLPGMEAAFVDIGCGRNAVLYSGEAVWEGVKAQSIENTVREGSSVLVQ
VTKDAVANKGPRVTGQLSLAGRYLVYAPGSGGVSRRIAPSERARLQKSLKAAVEDVSSLIIRTAAVGATSEQLGNDAKML
CEAYRAIKEKSENATAPSIIYSEPDLLIRLLRDTFNNDFKELIVEGAELYERVNSYLQDVSPEMLGKVSKSSEEDLFDAY
RITEQIDRSLNRKVLLPSGGSLVVDHTEAMTVIDVNTGKFVGTSSSLEQTVTDNNLEAVDEIVRQIRLRDLGGIVVIDFI
DMLLQENRDLVYNRLLEALSLDRSRHKVAEITSLGLVQMTRKKLGVALEPVSELCDRCSGRGRVINPVPNKPRARKKHRL
RQVNKEASDAAVRIISAMQDTAGYSDARSDTLSSRGALAPDSQVSISVGNRRRRLVTSTIDASGDPRAS
>Mature_549_residues
MFGRCMVDTGSKRASGYKVVFQAPDFSARIVSQKKRSDITPLRQSKRRASRTSPAETGILRRNTQRVMLVRGSGASDVQV
GILEEGILVEYYMARGASSSLVGNIYMGRVQNVLPGMEAAFVDIGCGRNAVLYSGEAVWEGVKAQSIENTVREGSSVLVQ
VTKDAVANKGPRVTGQLSLAGRYLVYAPGSGGVSRRIAPSERARLQKSLKAAVEDVSSLIIRTAAVGATSEQLGNDAKML
CEAYRAIKEKSENATAPSIIYSEPDLLIRLLRDTFNNDFKELIVEGAELYERVNSYLQDVSPEMLGKVSKSSEEDLFDAY
RITEQIDRSLNRKVLLPSGGSLVVDHTEAMTVIDVNTGKFVGTSSSLEQTVTDNNLEAVDEIVRQIRLRDLGGIVVIDFI
DMLLQENRDLVYNRLLEALSLDRSRHKVAEITSLGLVQMTRKKLGVALEPVSELCDRCSGRGRVINPVPNKPRARKKHRL
RQVNKEASDAAVRIISAMQDTAGYSDARSDTLSSRGALAPDSQVSISVGNRRRRLVTSTIDASGDPRAS

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=411, Percent_Identity=36.2530413625304, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082235, Length=404, Percent_Identity=39.3564356435644, Blast_Score=246, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 59970; Mature: 59970

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRCMVDTGSKRASGYKVVFQAPDFSARIVSQKKRSDITPLRQSKRRASRTSPAETGIL
CCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHH
RRNTQRVMLVRGSGASDVQVGILEEGILVEYYMARGASSSLVGNIYMGRVQNVLPGMEAA
CCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEE
FVDIGCGRNAVLYSGEAVWEGVKAQSIENTVREGSSVLVQVTKDAVANKGPRVTGQLSLA
EEEECCCCCEEEECCHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEEEEEC
GRYLVYAPGSGGVSRRIAPSERARLQKSLKAAVEDVSSLIIRTAAVGATSEQLGNDAKML
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHH
CEAYRAIKEKSENATAPSIIYSEPDLLIRLLRDTFNNDFKELIVEGAELYERVNSYLQDV
HHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
SPEMLGKVSKSSEEDLFDAYRITEQIDRSLNRKVLLPSGGSLVVDHTEAMTVIDVNTGKF
CHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEEECCCCCE
VGTSSSLEQTVTDNNLEAVDEIVRQIRLRDLGGIVVIDFIDMLLQENRDLVYNRLLEALS
ECCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LDRSRHKVAEITSLGLVQMTRKKLGVALEPVSELCDRCSGRGRVINPVPNKPRARKKHRL
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
RQVNKEASDAAVRIISAMQDTAGYSDARSDTLSSRGALAPDSQVSISVGNRRRRLVTSTI
HHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEC
DASGDPRAS
CCCCCCCCC
>Mature Secondary Structure
MFGRCMVDTGSKRASGYKVVFQAPDFSARIVSQKKRSDITPLRQSKRRASRTSPAETGIL
CCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHH
RRNTQRVMLVRGSGASDVQVGILEEGILVEYYMARGASSSLVGNIYMGRVQNVLPGMEAA
CCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEE
FVDIGCGRNAVLYSGEAVWEGVKAQSIENTVREGSSVLVQVTKDAVANKGPRVTGQLSLA
EEEECCCCCEEEECCHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEEEEEC
GRYLVYAPGSGGVSRRIAPSERARLQKSLKAAVEDVSSLIIRTAAVGATSEQLGNDAKML
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHH
CEAYRAIKEKSENATAPSIIYSEPDLLIRLLRDTFNNDFKELIVEGAELYERVNSYLQDV
HHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
SPEMLGKVSKSSEEDLFDAYRITEQIDRSLNRKVLLPSGGSLVVDHTEAMTVIDVNTGKF
CHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEEECCCCCE
VGTSSSLEQTVTDNNLEAVDEIVRQIRLRDLGGIVVIDFIDMLLQENRDLVYNRLLEALS
ECCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LDRSRHKVAEITSLGLVQMTRKKLGVALEPVSELCDRCSGRGRVINPVPNKPRARKKHRL
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
RQVNKEASDAAVRIISAMQDTAGYSDARSDTLSSRGALAPDSQVSISVGNRRRRLVTSTI
HHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEC
DASGDPRAS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]