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The map label for this gene is gyrB2 [H]

Identifier: 28572427

GI number: 28572427

Start: 301377

End: 303365

Strand: Direct

Name: gyrB2 [H]

Synonym: TW268

Alternate gene names: 28572427

Gene position: 301377-303365 (Clockwise)

Preceding gene: 28572424

Following gene: 28572428

Centisome position: 32.55

GC content: 45.9

Gene sequence:

>1989_bases
TTGTCGAGTAATACTGATTATAACGCCGATGACATTGCCCTACTAGAGGGCTTGGCTGCTGTTCGTAAACGGCCTGGAAT
GTACATAGGCTCAACCGACAATCACGGCTTGGCCCATTGTCTGTGGGAAATAATCGATAATTCTGTGGATGAGGTTTTAG
CTGGATTTGGCAATGTTGTGAACATTACCTTGCATGGCGATAACAGTTTTTCTGTTCAGGATTTTGGCCGAGGGATACCT
GTTGATATTGAAAGCAAGACGGGCCTAACGGGTGTTGAGTTGGTTTTTACAAAGCTTCATGCGGGCGGAAAGTTTGGCAC
AGGAGCGTATTTCACATCGGGTGGATTGCATGGTGTTGGTGCGAGTGTTGTAAATGCCCTTTCCGAAAAGCTCTCTGTTG
AGGTTGACAGAGATGGAATGACATGGGTAATGGAATTTCGTAACCAAGTGACATCTGGCCTTCGATCTAAGTGTAAAATC
GCTAGTAATGTAACGGGAACACGGGTGCGTTTTTGGCCTGATCCGCTTATTTTTGGTAGTGCAGAAATCTGTATTGAGTC
TTTGCATCAGCGCGCGATGCAAACTGCCTTTTTAGTTCCAGGCCTCAGGGTGAATATTGAAGATAGGGTGCTTGGCAAGA
GCTCTTCCTATCTCTTTGAGGGCGGTTGCAAACAATTTGTAGAGCATCTTTCCGCAGGAGAGCTGATTACAGATGTCTTG
TGTATTAGAGGTAAAGGTAGTTTTTCTGAGGCGGTTCCCGTTTTGAAAGAGACGGGTAACCTTGTTATCAGCTCAGTTGA
GCGGGTGTGTGATGTTGATATTGCTCTTGCGTGGCGTAATGATTTTGAAACTCATATCAATAGCTTTGTAAATGTGATCG
AAACCCCGAAGGGCGGAACCCATTATTCTGGTTTTGAGCAGGCTCTTTTGAAGACTATTCGAAACGCAGTGTCAAGTCAA
TCTAGGTATTTGAAGTCTGGCAATAACCGCCCCGAACGCGAAGATGTTTTTTCGGGACTGACCGCTGTGGTATCCGTAAA
ATTGCCCGAACCGCAATTTGAAGGCCAGACCAAGGAAGTGCTGGGAACACCCGCGGTGAAAGGCATAGTTGCAAATATCG
TGGCAGATAATCTTCAAAAACTTTTTGAAGATCCAAAATACAAAACCCAGATCCGGCGTGTCTTAGAAAAAGTTGTTGCA
CAAATGCACTTTCGGTTAGCCGCACGGGCTAGTAGGGAGACTCAGAGACGTAAGAATGCACTTGAAACTTCGAGTTTACC
CGCAAAACTTGTGGATTGTCGAAGCAATGATGTTAACGAAAGCGAACTGTTTATTGTTGAGGGAGATTCTGCCCTCGGCA
CTGCTCGCAGGGCCAGAAATAGCGAGTACCAGGCACTTCTGCCAATTCGAGGGAAAATCCTAAACGTACAAAAGGCGAGT
GTAGCCGATGCACTTGCTAATGCCGAATGTGCAAGTATTATTCAAGTGATTGGTGCCGGATCTGGAAGTAACTTTGATAT
AGCACAGGCACGCTATGGCAAGGTGATAATAATGTCTGATGCTGATGTGGATGGTGCTCACATTAGGATTCTGTTGCTAA
CCTTATTTTTCAGGTATATGCAGCCGATGATTTCATCCGGCCGTGTCTTTTCAGCCCTTCCTCCGTTACATCGGGTGGTG
GTTAAAAACAGCAATGAGAAGATCTACACCAGATCAGATCGTGAGCTAGAAATGGTTTTGTCAAGACTGAAACAGGAGGG
GCTTGAACATAGGGAGCCGATTCAGAGATACAAGGGCCTTGGTGAGATGGATGCCGATCAACTTGCAGAGACAACTATGG
ACAGATCAAAGCGTACCCTTCTTAGGATTAGGATGTCTGATGCCCAACAGGCTTCAGACATTTTTGCGCTCCTTATGGGG
AATGATGTCGCACCACGCAGGGAGTTCATAGCAAATTCCGTGGTTAGCACTGACAGAATAGACAGCTAA

Upstream 100 bases:

>100_bases
TTCTTTATACTCTCCAATGTAATAGTTGCTAAACGCGATATCATACTTTGACACAAAACACTTGTTTGATACTGACTTTT
AGAAGATTTCAGGAGCTATC

Downstream 100 bases:

>100_bases
GATTGCCTTACTGGCAGACCTTGTCAAGGATCCAGGCGATTTCCCAGGCTTGACTCTTCCAGATGTCATATCTGCCGCTT
GCGCCAAAATGTCCACATGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase II subunit B [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSSNTDYNADDIALLEGLAAVRKRPGMYIGSTDNHGLAHCLWEIIDNSVDEVLAGFGNVVNITLHGDNSFSVQDFGRGIP
VDIESKTGLTGVELVFTKLHAGGKFGTGAYFTSGGLHGVGASVVNALSEKLSVEVDRDGMTWVMEFRNQVTSGLRSKCKI
ASNVTGTRVRFWPDPLIFGSAEICIESLHQRAMQTAFLVPGLRVNIEDRVLGKSSSYLFEGGCKQFVEHLSAGELITDVL
CIRGKGSFSEAVPVLKETGNLVISSVERVCDVDIALAWRNDFETHINSFVNVIETPKGGTHYSGFEQALLKTIRNAVSSQ
SRYLKSGNNRPEREDVFSGLTAVVSVKLPEPQFEGQTKEVLGTPAVKGIVANIVADNLQKLFEDPKYKTQIRRVLEKVVA
QMHFRLAARASRETQRRKNALETSSLPAKLVDCRSNDVNESELFIVEGDSALGTARRARNSEYQALLPIRGKILNVQKAS
VADALANAECASIIQVIGAGSGSNFDIAQARYGKVIIMSDADVDGAHIRILLLTLFFRYMQPMISSGRVFSALPPLHRVV
VKNSNEKIYTRSDRELEMVLSRLKQEGLEHREPIQRYKGLGEMDADQLAETTMDRSKRTLLRIRMSDAQQASDIFALLMG
NDVAPRREFIANSVVSTDRIDS

Sequences:

>Translated_662_residues
MSSNTDYNADDIALLEGLAAVRKRPGMYIGSTDNHGLAHCLWEIIDNSVDEVLAGFGNVVNITLHGDNSFSVQDFGRGIP
VDIESKTGLTGVELVFTKLHAGGKFGTGAYFTSGGLHGVGASVVNALSEKLSVEVDRDGMTWVMEFRNQVTSGLRSKCKI
ASNVTGTRVRFWPDPLIFGSAEICIESLHQRAMQTAFLVPGLRVNIEDRVLGKSSSYLFEGGCKQFVEHLSAGELITDVL
CIRGKGSFSEAVPVLKETGNLVISSVERVCDVDIALAWRNDFETHINSFVNVIETPKGGTHYSGFEQALLKTIRNAVSSQ
SRYLKSGNNRPEREDVFSGLTAVVSVKLPEPQFEGQTKEVLGTPAVKGIVANIVADNLQKLFEDPKYKTQIRRVLEKVVA
QMHFRLAARASRETQRRKNALETSSLPAKLVDCRSNDVNESELFIVEGDSALGTARRARNSEYQALLPIRGKILNVQKAS
VADALANAECASIIQVIGAGSGSNFDIAQARYGKVIIMSDADVDGAHIRILLLTLFFRYMQPMISSGRVFSALPPLHRVV
VKNSNEKIYTRSDRELEMVLSRLKQEGLEHREPIQRYKGLGEMDADQLAETTMDRSKRTLLRIRMSDAQQASDIFALLMG
NDVAPRREFIANSVVSTDRIDS
>Mature_661_residues
SSNTDYNADDIALLEGLAAVRKRPGMYIGSTDNHGLAHCLWEIIDNSVDEVLAGFGNVVNITLHGDNSFSVQDFGRGIPV
DIESKTGLTGVELVFTKLHAGGKFGTGAYFTSGGLHGVGASVVNALSEKLSVEVDRDGMTWVMEFRNQVTSGLRSKCKIA
SNVTGTRVRFWPDPLIFGSAEICIESLHQRAMQTAFLVPGLRVNIEDRVLGKSSSYLFEGGCKQFVEHLSAGELITDVLC
IRGKGSFSEAVPVLKETGNLVISSVERVCDVDIALAWRNDFETHINSFVNVIETPKGGTHYSGFEQALLKTIRNAVSSQS
RYLKSGNNRPEREDVFSGLTAVVSVKLPEPQFEGQTKEVLGTPAVKGIVANIVADNLQKLFEDPKYKTQIRRVLEKVVAQ
MHFRLAARASRETQRRKNALETSSLPAKLVDCRSNDVNESELFIVEGDSALGTARRARNSEYQALLPIRGKILNVQKASV
ADALANAECASIIQVIGAGSGSNFDIAQARYGKVIIMSDADVDGAHIRILLLTLFFRYMQPMISSGRVFSALPPLHRVVV
KNSNEKIYTRSDRELEMVLSRLKQEGLEHREPIQRYKGLGEMDADQLAETTMDRSKRTLLRIRMSDAQQASDIFALLMGN
DVAPRREFIANSVVSTDRIDS

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=685, Percent_Identity=24.963503649635, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI19913406, Length=691, Percent_Identity=23.0101302460203, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI48994957, Length=580, Percent_Identity=40.5172413793103, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=37.5796178343949, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI212645845, Length=576, Percent_Identity=24.1319444444444, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535065, Length=687, Percent_Identity=21.688500727802, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324241, Length=701, Percent_Identity=22.2539229671897, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17136538, Length=653, Percent_Identity=24.0428790199081, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72718; Mature: 72587

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNTDYNADDIALLEGLAAVRKRPGMYIGSTDNHGLAHCLWEIIDNSVDEVLAGFGNVV
CCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCEE
NITLHGDNSFSVQDFGRGIPVDIESKTGLTGVELVFTKLHAGGKFGTGAYFTSGGLHGVG
EEEEECCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
ASVVNALSEKLSVEVDRDGMTWVMEFRNQVTSGLRSKCKIASNVTGTRVRFWPDPLIFGS
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECC
AEICIESLHQRAMQTAFLVPGLRVNIEDRVLGKSSSYLFEGGCKQFVEHLSAGELITDVL
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
CIRGKGSFSEAVPVLKETGNLVISSVERVCDVDIALAWRNDFETHINSFVNVIETPKGGT
HHCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCC
HYSGFEQALLKTIRNAVSSQSRYLKSGNNRPEREDVFSGLTAVVSVKLPEPQFEGQTKEV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCHHHH
LGTPAVKGIVANIVADNLQKLFEDPKYKTQIRRVLEKVVAQMHFRLAARASRETQRRKNA
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETSSLPAKLVDCRSNDVNESELFIVEGDSALGTARRARNSEYQALLPIRGKILNVQKAS
HHHCCCCHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEECHHHH
VADALANAECASIIQVIGAGSGSNFDIAQARYGKVIIMSDADVDGAHIRILLLTLFFRYM
HHHHHHCHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
QPMISSGRVFSALPPLHRVVVKNSNEKIYTRSDRELEMVLSRLKQEGLEHREPIQRYKGL
HHHHHCCCEEHHCCHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCC
GEMDADQLAETTMDRSKRTLLRIRMSDAQQASDIFALLMGNDVAPRREFIANSVVSTDRI
CCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
DS
CC
>Mature Secondary Structure 
SSNTDYNADDIALLEGLAAVRKRPGMYIGSTDNHGLAHCLWEIIDNSVDEVLAGFGNVV
CCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCEE
NITLHGDNSFSVQDFGRGIPVDIESKTGLTGVELVFTKLHAGGKFGTGAYFTSGGLHGVG
EEEEECCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
ASVVNALSEKLSVEVDRDGMTWVMEFRNQVTSGLRSKCKIASNVTGTRVRFWPDPLIFGS
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECC
AEICIESLHQRAMQTAFLVPGLRVNIEDRVLGKSSSYLFEGGCKQFVEHLSAGELITDVL
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
CIRGKGSFSEAVPVLKETGNLVISSVERVCDVDIALAWRNDFETHINSFVNVIETPKGGT
HHCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCC
HYSGFEQALLKTIRNAVSSQSRYLKSGNNRPEREDVFSGLTAVVSVKLPEPQFEGQTKEV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCHHHH
LGTPAVKGIVANIVADNLQKLFEDPKYKTQIRRVLEKVVAQMHFRLAARASRETQRRKNA
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETSSLPAKLVDCRSNDVNESELFIVEGDSALGTARRARNSEYQALLPIRGKILNVQKAS
HHHCCCCHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEECHHHH
VADALANAECASIIQVIGAGSGSNFDIAQARYGKVIIMSDADVDGAHIRILLLTLFFRYM
HHHHHHCHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
QPMISSGRVFSALPPLHRVVVKNSNEKIYTRSDRELEMVLSRLKQEGLEHREPIQRYKGL
HHHHHCCCEEHHCCHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCC
GEMDADQLAETTMDRSKRTLLRIRMSDAQQASDIFALLMGNDVAPRREFIANSVVSTDRI
CCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
DS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8863738; 10360571 [H]