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The map label for this gene is sucB [H]

Identifier: 28572418

GI number: 28572418

Start: 288527

End: 289912

Strand: Reverse

Name: sucB [H]

Synonym: TW259

Alternate gene names: 28572418

Gene position: 289912-288527 (Counterclockwise)

Preceding gene: 28572419

Following gene: 28572417

Centisome position: 31.31

GC content: 44.73

Gene sequence:

>1386_bases
ATGAGTGAGGACTTTATCCTGCCCGCATTGGGTGAAAGCGTAAGTGAGTGCGTCATAACTCGCTGGCTCAAAGAAGCTGG
GGATCGGGTTGAAGTTGATGAACCACTGGTTGAAGTCTCAACGGATAAAGTTGATACCGAGCTTCCGTCCACTTTAACTG
GGATATTGGAAGAAATACTTGTTCAGCGCGATGAAACAGCAAAACCTGGGCAGATTCTCGCTAGGATAGCAGTCGATAAA
GATGAGACGAAAAGCAATTTTGACCACGGGGTAAAACAATCAGATGTAATAACAGGGGATTCCGCCGGTAACCTGTTGGG
TAATACTCGTCCAATCGAAGATAATGAATGGCCAGTCACACTGCATACCGCAGAGAGCAAACCGGATCTATCAGCAAAAG
CAGATCATATTGGAGATGAAGGCGACAAGCAAGGCAATTTGTATGCTGCGCATAATGCAGAAATACCGTACGCAACACCG
ATTGTTAGACAGCTTGCAAGAAAACTGAATATTGATCTAACTAACATAGTCGGGAGCGGAGTGGGCAACAGAATCCTAAG
GGAGGATGTATTGTTGGCGAGTAACCGCACAACAAATACCTCAACCGAATGTGCTCAAAACATCCCATCAAGTCCAGAAC
ATGACAAAAGTGCCGCAAGCTTTGACAGCGAAGTATCAGAACTCCGTGGGACGCGGATTAAGATGTCTCGCCTGCGCAAC
ATAATAGCTGAGAGGGCTGTTCATTCAATGCAGAACACCGCACAGCTTAGCACTGTAATTGAGGTAGATCTAACAAGGAT
AGTTGACTATCGCAATAGCATAAAAGAAAAATTCAAAGAAGCGGAGGGTGTGAATCTAACCGTCCTACCGTTTATAATCA
ACGCCACAGTTCAAGCACTTAGAAAATATCCAGTTATTAATTCCCATATTGTGGATGATCAAATAGTTTTTCCGGATTAT
GAAAATATTTCACTTGCTGTTGACACCGAAAGAGGGTTGCTGACACCCGTAATAAAAAACGCTGGAGATATGACGGTGGC
GCAATTTGCAAAAAGTGTTTTTGATCTGGCGCGAAGGGCACGTAACAACAAACTATCTCCAGATGAACTTACCGGTGGAA
CATTTACAGTCACGAATACCGGTTCCCGAGGTGCTCTATTTGATACGCCGGTTGTATTTCTTCCGCAACTCGCGATTTTG
GGAATTGGTGCTATTGCAAGGCGTCCGGTAATTGTGTTAGACGCGCAAGGAAACGAGTGTATCTCGATAAGATCCGTGGC
GTTTTTTGCCCTATCGTACGACCATAGGGTTATTGATGGCGCGGATGCTGCTAGGTTTTTAGGATACATAAAATCCCTGC
TTGAAACGGGAAATTGTACAGTTTAG

Upstream 100 bases:

>100_bases
CGCGTAACTTTAAGGCAAAATGCGGCCCACAAAAACCTTTGAAAATGCGGCGCTTGTGCGGGATGTGTGTTACATATGGT
TATAATCCTGGAGACTTTGA

Downstream 100 bases:

>100_bases
CGTGCAATGGATTTAAGGTTGCTACGATGGGAGTTTCCTGCAAAACCGTGTGTGCCTTTATTGTTAGCTCTGCGCTTTTT
GGCTGCAGCACGGCTATTGT

Product: lipoamide acyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSEDFILPALGESVSECVITRWLKEAGDRVEVDEPLVEVSTDKVDTELPSTLTGILEEILVQRDETAKPGQILARIAVDK
DETKSNFDHGVKQSDVITGDSAGNLLGNTRPIEDNEWPVTLHTAESKPDLSAKADHIGDEGDKQGNLYAAHNAEIPYATP
IVRQLARKLNIDLTNIVGSGVGNRILREDVLLASNRTTNTSTECAQNIPSSPEHDKSAASFDSEVSELRGTRIKMSRLRN
IIAERAVHSMQNTAQLSTVIEVDLTRIVDYRNSIKEKFKEAEGVNLTVLPFIINATVQALRKYPVINSHIVDDQIVFPDY
ENISLAVDTERGLLTPVIKNAGDMTVAQFAKSVFDLARRARNNKLSPDELTGGTFTVTNTGSRGALFDTPVVFLPQLAIL
GIGAIARRPVIVLDAQGNECISIRSVAFFALSYDHRVIDGADAARFLGYIKSLLETGNCTV

Sequences:

>Translated_461_residues
MSEDFILPALGESVSECVITRWLKEAGDRVEVDEPLVEVSTDKVDTELPSTLTGILEEILVQRDETAKPGQILARIAVDK
DETKSNFDHGVKQSDVITGDSAGNLLGNTRPIEDNEWPVTLHTAESKPDLSAKADHIGDEGDKQGNLYAAHNAEIPYATP
IVRQLARKLNIDLTNIVGSGVGNRILREDVLLASNRTTNTSTECAQNIPSSPEHDKSAASFDSEVSELRGTRIKMSRLRN
IIAERAVHSMQNTAQLSTVIEVDLTRIVDYRNSIKEKFKEAEGVNLTVLPFIINATVQALRKYPVINSHIVDDQIVFPDY
ENISLAVDTERGLLTPVIKNAGDMTVAQFAKSVFDLARRARNNKLSPDELTGGTFTVTNTGSRGALFDTPVVFLPQLAIL
GIGAIARRPVIVLDAQGNECISIRSVAFFALSYDHRVIDGADAARFLGYIKSLLETGNCTV
>Mature_460_residues
SEDFILPALGESVSECVITRWLKEAGDRVEVDEPLVEVSTDKVDTELPSTLTGILEEILVQRDETAKPGQILARIAVDKD
ETKSNFDHGVKQSDVITGDSAGNLLGNTRPIEDNEWPVTLHTAESKPDLSAKADHIGDEGDKQGNLYAAHNAEIPYATPI
VRQLARKLNIDLTNIVGSGVGNRILREDVLLASNRTTNTSTECAQNIPSSPEHDKSAASFDSEVSELRGTRIKMSRLRNI
IAERAVHSMQNTAQLSTVIEVDLTRIVDYRNSIKEKFKEAEGVNLTVLPFIINATVQALRKYPVINSHIVDDQIVFPDYE
NISLAVDTERGLLTPVIKNAGDMTVAQFAKSVFDLARRARNNKLSPDELTGGTFTVTNTGSRGALFDTPVVFLPQLAILG
IGAIARRPVIVLDAQGNECISIRSVAFFALSYDHRVIDGADAARFLGYIKSLLETGNCTV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=38.7665198237885, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI110671329, Length=467, Percent_Identity=26.9807280513919, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI31711992, Length=454, Percent_Identity=28.1938325991189, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI203098816, Length=473, Percent_Identity=26.6384778012685, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI203098753, Length=473, Percent_Identity=26.6384778012685, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI260898739, Length=138, Percent_Identity=40.5797101449275, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786946, Length=454, Percent_Identity=33.7004405286344, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1786305, Length=315, Percent_Identity=31.7460317460317, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=224, Percent_Identity=36.1607142857143, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=464, Percent_Identity=29.0948275862069, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=460, Percent_Identity=27.3913043478261, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=216, Percent_Identity=31.4814814814815, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=225, Percent_Identity=38.6666666666667, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=26.304347826087, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=38.3928571428571, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 50330; Mature: 50199

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDFILPALGESVSECVITRWLKEAGDRVEVDEPLVEVSTDKVDTELPSTLTGILEEIL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHCCCCCHHHHHCHHHHHHHHHHHH
VQRDETAKPGQILARIAVDKDETKSNFDHGVKQSDVITGDSAGNLLGNTRPIEDNEWPVT
HCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEE
LHTAESKPDLSAKADHIGDEGDKQGNLYAAHNAEIPYATPIVRQLARKLNIDLTNIVGSG
EEECCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
VGNRILREDVLLASNRTTNTSTECAQNIPSSPEHDKSAASFDSEVSELRGTRIKMSRLRN
CCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIAERAVHSMQNTAQLSTVIEVDLTRIVDYRNSIKEKFKEAEGVNLTVLPFIINATVQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
RKYPVINSHIVDDQIVFPDYENISLAVDTERGLLTPVIKNAGDMTVAQFAKSVFDLARRA
HHCCCCCCEECCCEEECCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
RNNKLSPDELTGGTFTVTNTGSRGALFDTPVVFLPQLAILGIGAIARRPVIVLDAQGNEC
HCCCCCCHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
ISIRSVAFFALSYDHRVIDGADAARFLGYIKSLLETGNCTV
EEHHHHEEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEDFILPALGESVSECVITRWLKEAGDRVEVDEPLVEVSTDKVDTELPSTLTGILEEIL
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHCCCCCHHHHHCHHHHHHHHHHHH
VQRDETAKPGQILARIAVDKDETKSNFDHGVKQSDVITGDSAGNLLGNTRPIEDNEWPVT
HCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEE
LHTAESKPDLSAKADHIGDEGDKQGNLYAAHNAEIPYATPIVRQLARKLNIDLTNIVGSG
EEECCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
VGNRILREDVLLASNRTTNTSTECAQNIPSSPEHDKSAASFDSEVSELRGTRIKMSRLRN
CCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIAERAVHSMQNTAQLSTVIEVDLTRIVDYRNSIKEKFKEAEGVNLTVLPFIINATVQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
RKYPVINSHIVDDQIVFPDYENISLAVDTERGLLTPVIKNAGDMTVAQFAKSVFDLARRA
HHCCCCCCEECCCEEECCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
RNNKLSPDELTGGTFTVTNTGSRGALFDTPVVFLPQLAILGIGAIARRPVIVLDAQGNEC
HCCCCCCHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
ISIRSVAFFALSYDHRVIDGADAARFLGYIKSLLETGNCTV
EEHHHHEEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]