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The map label for this gene is acoL [H]

Identifier: 28572419

GI number: 28572419

Start: 290066

End: 291418

Strand: Reverse

Name: acoL [H]

Synonym: TW260

Alternate gene names: 28572419

Gene position: 291418-290066 (Counterclockwise)

Preceding gene: 28572420

Following gene: 28572418

Centisome position: 31.47

GC content: 46.56

Gene sequence:

>1353_bases
ATGGACTACGATCTGATAGTTCTTGGTGCGGGAAGTGCCGGTTACGCGACTGCTATACGCGCAACGCAATTAGGTATGAA
GGTTGCACTTGCTGAGGGGGATAAAGTTGGCGGGACATGCCTGCATAGGGGATGTATCCCAACAAAATCGCTTGTACAAT
GTGCAAAAGCTGCCCATATTGCCAAAAATGCGTCAAAATTTGGGGTTATAAATAGTTTTTCTGGTGTTGATATAAATGCT
GTAAATGTCTTCAAGAATGGCATTGTTGAAAGTAAGTACCGTGGGCTACAGTCACTTTTGAGATCCTACGGTATCCATGT
CTACCCGAATTTTGCAACGCTTAGTTCACAAAACTCTGTTCGGATCGGCACAGAAGATATTACAGGGAAAAACATAGTGA
TCGCAACAGGTTCTAGACCGAAAGGCATACCGGGATTAAGCGGGGGGTGCATCTTTGACAGCGATTCTATTCTCGAGATA
GATCATCTCCCGTCGAGTCTTGCCATTATTGGCGGGGGTGTAATAGGCGTCGAGTTTGCAAGTATCTTTAATTATCTCGG
TGTTGATGTGAGCATACTCGAGGCTCAAGAAACTCTTATTCCTTCCGAGGAGCGAGGTGTTAGCAAACAGCTCGAACGGG
TTTTCAGGCGAAGAGGTATAAAGCTTTATCTGGGGCACAAAATAATGGATGTGTCACAGACAGATGGTGTGGTGGTATCC
CTTGATTCAAACGAAAAAGTAAATGCTGACCTGCTTCTGGTGGCAATTGGCAGGGCACCCGCAACAGATGCAATAGGCAA
AGTTGGAATAGAAATTGATGGAGGTGCCATCTCTGTTGATGAAAAACTAAGGACAACTGTGCCTAATGTATTCGCTGCGG
GAGATGTTGTAGGTGGTCTACAGCTTGCGCACAGGGGGTATCAGCAGGGCATATATTTGGCTGAACAAATTGCTGGTCTT
GACCCATCCGCTGTGGCAGACATTAATATCCCGAGGGTGATTTACACAAGCCCAGAAGTCGCATCTGTCGGATATACAGA
AGCTGGGGCAGCAGAGATATATGGCGTGAATGAAATAGTTACCTATGAATACAATCTCGCTGGCAATGCAAAAAGTTCCA
TTATGGGGGCTGCGGGAAGCATTAAGGTGGTCCAGCACAAAAACAAGGACGTCCTTGGGGTTCATATGGTCGGCGATGGG
GTTAGTGAGCTTGTATCCGAGGCTCAATTGATTGTAAATTGGGAAGCCAACCCATCAGATGTTGCATCGCTTATTCATCC
ACATCCAACCCAGGCAGAAGCTATCGGCGAAGCGATGCTTGCACTCGCAGGGAAACCCCTACATGGTATTTAG

Upstream 100 bases:

>100_bases
ATTTAATTCTTGCGGCCCGACTGGCGTGGCTGTAAGGTCCCTTGTTGAATTTTGCCGATGCCTGTCTTCTGCTCCAGAGC
GAAAGCTGTTCTAGGGCATT

Downstream 100 bases:

>100_bases
TACCTGGGCATTACAAGAAATATCATTTATCGCGTGGTTGCGTAAATGCAGACCGCGTAACTTTAAGGCAAAATGCGGCC
CACAAAAACCTTTGAAAATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDYDLIVLGAGSAGYATAIRATQLGMKVALAEGDKVGGTCLHRGCIPTKSLVQCAKAAHIAKNASKFGVINSFSGVDINA
VNVFKNGIVESKYRGLQSLLRSYGIHVYPNFATLSSQNSVRIGTEDITGKNIVIATGSRPKGIPGLSGGCIFDSDSILEI
DHLPSSLAIIGGGVIGVEFASIFNYLGVDVSILEAQETLIPSEERGVSKQLERVFRRRGIKLYLGHKIMDVSQTDGVVVS
LDSNEKVNADLLLVAIGRAPATDAIGKVGIEIDGGAISVDEKLRTTVPNVFAAGDVVGGLQLAHRGYQQGIYLAEQIAGL
DPSAVADINIPRVIYTSPEVASVGYTEAGAAEIYGVNEIVTYEYNLAGNAKSSIMGAAGSIKVVQHKNKDVLGVHMVGDG
VSELVSEAQLIVNWEANPSDVASLIHPHPTQAEAIGEAMLALAGKPLHGI

Sequences:

>Translated_450_residues
MDYDLIVLGAGSAGYATAIRATQLGMKVALAEGDKVGGTCLHRGCIPTKSLVQCAKAAHIAKNASKFGVINSFSGVDINA
VNVFKNGIVESKYRGLQSLLRSYGIHVYPNFATLSSQNSVRIGTEDITGKNIVIATGSRPKGIPGLSGGCIFDSDSILEI
DHLPSSLAIIGGGVIGVEFASIFNYLGVDVSILEAQETLIPSEERGVSKQLERVFRRRGIKLYLGHKIMDVSQTDGVVVS
LDSNEKVNADLLLVAIGRAPATDAIGKVGIEIDGGAISVDEKLRTTVPNVFAAGDVVGGLQLAHRGYQQGIYLAEQIAGL
DPSAVADINIPRVIYTSPEVASVGYTEAGAAEIYGVNEIVTYEYNLAGNAKSSIMGAAGSIKVVQHKNKDVLGVHMVGDG
VSELVSEAQLIVNWEANPSDVASLIHPHPTQAEAIGEAMLALAGKPLHGI
>Mature_450_residues
MDYDLIVLGAGSAGYATAIRATQLGMKVALAEGDKVGGTCLHRGCIPTKSLVQCAKAAHIAKNASKFGVINSFSGVDINA
VNVFKNGIVESKYRGLQSLLRSYGIHVYPNFATLSSQNSVRIGTEDITGKNIVIATGSRPKGIPGLSGGCIFDSDSILEI
DHLPSSLAIIGGGVIGVEFASIFNYLGVDVSILEAQETLIPSEERGVSKQLERVFRRRGIKLYLGHKIMDVSQTDGVVVS
LDSNEKVNADLLLVAIGRAPATDAIGKVGIEIDGGAISVDEKLRTTVPNVFAAGDVVGGLQLAHRGYQQGIYLAEQIAGL
DPSAVADINIPRVIYTSPEVASVGYTEAGAAEIYGVNEIVTYEYNLAGNAKSSIMGAAGSIKVVQHKNKDVLGVHMVGDG
VSELVSEAQLIVNWEANPSDVASLIHPHPTQAEAIGEAMLALAGKPLHGI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=34.5263157894737, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI50301238, Length=456, Percent_Identity=27.8508771929825, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI291045266, Length=446, Percent_Identity=25.1121076233184, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI22035672, Length=479, Percent_Identity=26.0960334029228, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148277065, Length=475, Percent_Identity=25.6842105263158, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI33519430, Length=475, Percent_Identity=25.6842105263158, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI33519428, Length=475, Percent_Identity=25.6842105263158, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI33519426, Length=475, Percent_Identity=25.6842105263158, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI148277071, Length=475, Percent_Identity=25.6842105263158, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI291045268, Length=350, Percent_Identity=26, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI21389617, Length=234, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI65787454, Length=234, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI226437568, Length=234, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=32.6797385620915, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=31.1015118790497, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=29.490022172949, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789915, Length=425, Percent_Identity=27.2941176470588, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=33.4038054968288, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=27.2331154684096, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=27.2331154684096, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=26.5263157894737, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=475, Percent_Identity=25.8947368421053, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=31.0126582278481, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=26.9067796610169, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=34.2494714587738, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=25, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=25, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24640551, Length=482, Percent_Identity=25.7261410788382, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=24.2105263157895, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47080; Mature: 47080

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYDLIVLGAGSAGYATAIRATQLGMKVALAEGDKVGGTCLHRGCIPTKSLVQCAKAAHI
CCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHEECCCCCHHHHHHHHHHHHH
AKNASKFGVINSFSGVDINAVNVFKNGIVESKYRGLQSLLRSYGIHVYPNFATLSSQNSV
HHCCHHCCEEECCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCEEEECEEEECCCCCE
RIGTEDITGKNIVIATGSRPKGIPGLSGGCIFDSDSILEIDHLPSSLAIIGGGVIGVEFA
EECCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEECCCCCCEEEEECCCHHHHHH
SIFNYLGVDVSILEAQETLIPSEERGVSKQLERVFRRRGIKLYLGHKIMDVSQTDGVVVS
HHHHHHCCCEEEEEHHHHHCCCHHCCHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEE
LDSNEKVNADLLLVAIGRAPATDAIGKVGIEIDGGAISVDEKLRTTVPNVFAAGDVVGGL
ECCCCCCCCEEEEEEECCCCCCCCHHEEEEEECCCEEEECHHHHHHCCCCEECCCHHHHH
QLAHRGYQQGIYLAEQIAGLDPSAVADINIPRVIYTSPEVASVGYTEAGAAEIYGVNEIV
HHHHHHHHCCHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCCCCCCCCEEEEECCEEE
TYEYNLAGNAKSSIMGAAGSIKVVQHKNKDVLGVHMVGDGVSELVSEAQLIVNWEANPSD
EEEEECCCCCHHHHCCCCCCEEEEEECCCCEEEEEEECCCHHHHHCCCEEEEEECCCHHH
VASLIHPHPTQAEAIGEAMLALAGKPLHGI
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYDLIVLGAGSAGYATAIRATQLGMKVALAEGDKVGGTCLHRGCIPTKSLVQCAKAAHI
CCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHEECCCCCHHHHHHHHHHHHH
AKNASKFGVINSFSGVDINAVNVFKNGIVESKYRGLQSLLRSYGIHVYPNFATLSSQNSV
HHCCHHCCEEECCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCEEEECEEEECCCCCE
RIGTEDITGKNIVIATGSRPKGIPGLSGGCIFDSDSILEIDHLPSSLAIIGGGVIGVEFA
EECCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEECCCCCCEEEEECCCHHHHHH
SIFNYLGVDVSILEAQETLIPSEERGVSKQLERVFRRRGIKLYLGHKIMDVSQTDGVVVS
HHHHHHCCCEEEEEHHHHHCCCHHCCHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEE
LDSNEKVNADLLLVAIGRAPATDAIGKVGIEIDGGAISVDEKLRTTVPNVFAAGDVVGGL
ECCCCCCCCEEEEEEECCCCCCCCHHEEEEEECCCEEEECHHHHHHCCCCEECCCHHHHH
QLAHRGYQQGIYLAEQIAGLDPSAVADINIPRVIYTSPEVASVGYTEAGAAEIYGVNEIV
HHHHHHHHCCHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCCCCCCCCEEEEECCEEE
TYEYNLAGNAKSSIMGAAGSIKVVQHKNKDVLGVHMVGDGVSELVSEAQLIVNWEANPSD
EEEEECCCCCHHHHCCCCCCEEEEEECCCCEEEEEEECCCHHHHHCCCEEEEEECCCHHH
VASLIHPHPTQAEAIGEAMLALAGKPLHGI
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]