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The map label for this gene is aroA

Identifier: 28572354

GI number: 28572354

Start: 231435

End: 232766

Strand: Reverse

Name: aroA

Synonym: TW194

Alternate gene names: 28572354

Gene position: 232766-231435 (Counterclockwise)

Preceding gene: 28572357

Following gene: 28572350

Centisome position: 25.14

GC content: 44.89

Gene sequence:

>1332_bases
ATGGCGAAAACTGAAATGTACCAACCACCTCTAGGCAATAGAGCACTCTTTAAGATGTCGATACCCGGATCAAAATCTCT
AACAAATAGACACTTGATAATTGCAGCGATTGCGAGTGGGGAAACTACAATACATAACTTGCTTGAGTCTCGTGATACGA
ACCTCATGATCGAAGGTCTAAGGCGTATCGGGTGCAAAATAGAGAAGCTCAATCACACCGGTACGCATGATACCGGCGTG
ATATCGCCACACTGTACATGCCTCAACGATCTGATACAGCCGAGCGACGTGAGGATAATCCCATCAAAGCATTACACCTG
CAGCACAAAAATTGACTGTGGCCTTGCCGGTACCGTAATGCGATTTTTACCGGTTTTGGCAGGGCTATGCAAGGGATCAG
TTGAATTTTTCGGTGACGATCAGGCAATTCGAAGACCAATGGATGGAACACTTCATGCTCTAAGAAAACTTGGTGTTCAA
GTTGATGGGGATCGTATTCCATTTACCGTACACGGAAGGGGGGAGATAGAGGGTGGGGCTCTTGAAACCACCGAACATTC
TTCAAGTCAATTTATCTCCGGTCTTCTTTTATCTGCATGCAGATTCAAAAATGGGCTTACTCTAAAACATATCGGTAACC
CCCTTCCAAGCCGTCCATATATCGATATGACAGTTGAAGTTATGCGCGAGTGGGGAATCAATGTAACACACTCCGATGGT
GTATGGGCAGTAACACCCAAAGAATTAACTGGTAAACATATCACCATTGAGCCAGATCTATCCAATGCGGCTCCATTTAT
GATTGGTGCAATTGTTACCGGGGGCAGTGCAACGATACAGAACTGGCCAAGCAAAACATCGCAACCTGGAAAATATTTGG
AAGCTATTTTGCCGCAGTTTGGTGCGGAGATAACAAAAACCGCAAATACTATTACCGTCAGTGGAACAGGAAATATAACA
GGAATTAGGGCAGATCTTGGGCATATTGGAGAGCTTGTGCCCAATCTTGTTGCACTGGCCACTCTGGCAGAAACGCCAAG
TGTGTTTTATAACATAGGCCACATCCGATATCACGAAACTGACAGAATTGAAGCGCTTGTTAATGAAATAAGTTCACTGG
GCGGGACGATAACAGCTGGGAAAGACTATATAAAAATCACCCCAACCACTCTTACAAGAAGTGGTGTATGGAAAACCTAC
AAAGACCACAGAATGGCAACGTCAGGCGCAATAATCGGCCTTCGTCATAAACTGACTATAGAAGATATCGAGTGCACGAG
TAAAACATTCCCTCGGTTTGCAGATCTCTGGAGCGGTGCGTTTGGTAAATGA

Upstream 100 bases:

>100_bases
AAACTCTTTGGCTAATAGTGATGCCATTTTTTTGCCAGTGTGGACAGACACATCACTGAAAAAAACATGCTCCATTACTA
CAATTTTAGTGGGCAACAAG

Downstream 100 bases:

>100_bases
TAAAGAAAAATCCGGACAAACGCCTATTCATGGCCTAATGAGCATGCAGAGGACTTTGCTAAGAGGTTCGATCAATTACT
TGATAAGACCCTAAAGGGTG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAKTEMYQPPLGNRALFKMSIPGSKSLTNRHLIIAAIASGETTIHNLLESRDTNLMIEGLRRIGCKIEKLNHTGTHDTGV
ISPHCTCLNDLIQPSDVRIIPSKHYTCSTKIDCGLAGTVMRFLPVLAGLCKGSVEFFGDDQAIRRPMDGTLHALRKLGVQ
VDGDRIPFTVHGRGEIEGGALETTEHSSSQFISGLLLSACRFKNGLTLKHIGNPLPSRPYIDMTVEVMREWGINVTHSDG
VWAVTPKELTGKHITIEPDLSNAAPFMIGAIVTGGSATIQNWPSKTSQPGKYLEAILPQFGAEITKTANTITVSGTGNIT
GIRADLGHIGELVPNLVALATLAETPSVFYNIGHIRYHETDRIEALVNEISSLGGTITAGKDYIKITPTTLTRSGVWKTY
KDHRMATSGAIIGLRHKLTIEDIECTSKTFPRFADLWSGAFGK

Sequences:

>Translated_443_residues
MAKTEMYQPPLGNRALFKMSIPGSKSLTNRHLIIAAIASGETTIHNLLESRDTNLMIEGLRRIGCKIEKLNHTGTHDTGV
ISPHCTCLNDLIQPSDVRIIPSKHYTCSTKIDCGLAGTVMRFLPVLAGLCKGSVEFFGDDQAIRRPMDGTLHALRKLGVQ
VDGDRIPFTVHGRGEIEGGALETTEHSSSQFISGLLLSACRFKNGLTLKHIGNPLPSRPYIDMTVEVMREWGINVTHSDG
VWAVTPKELTGKHITIEPDLSNAAPFMIGAIVTGGSATIQNWPSKTSQPGKYLEAILPQFGAEITKTANTITVSGTGNIT
GIRADLGHIGELVPNLVALATLAETPSVFYNIGHIRYHETDRIEALVNEISSLGGTITAGKDYIKITPTTLTRSGVWKTY
KDHRMATSGAIIGLRHKLTIEDIECTSKTFPRFADLWSGAFGK
>Mature_442_residues
AKTEMYQPPLGNRALFKMSIPGSKSLTNRHLIIAAIASGETTIHNLLESRDTNLMIEGLRRIGCKIEKLNHTGTHDTGVI
SPHCTCLNDLIQPSDVRIIPSKHYTCSTKIDCGLAGTVMRFLPVLAGLCKGSVEFFGDDQAIRRPMDGTLHALRKLGVQV
DGDRIPFTVHGRGEIEGGALETTEHSSSQFISGLLLSACRFKNGLTLKHIGNPLPSRPYIDMTVEVMREWGINVTHSDGV
WAVTPKELTGKHITIEPDLSNAAPFMIGAIVTGGSATIQNWPSKTSQPGKYLEAILPQFGAEITKTANTITVSGTGNITG
IRADLGHIGELVPNLVALATLAETPSVFYNIGHIRYHETDRIEALVNEISSLGGTITAGKDYIKITPTTLTRSGVWKTYK
DHRMATSGAIIGLRHKLTIEDIECTSKTFPRFADLWSGAFGK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=425, Percent_Identity=28.7058823529412, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320332, Length=469, Percent_Identity=26.226012793177, Blast_Score=122, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_TROW8 (Q83I86)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789134.1
- HSSP:   P22487
- ProteinModelPortal:   Q83I86
- STRING:   Q83I86
- GeneID:   1064486
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW194
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   HRMATAG
- PhylomeDB:   Q83I86
- ProtClustDB:   PRK02427
- BioCyc:   TWHI218496:TW0179-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 48073; Mature: 47942

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTEMYQPPLGNRALFKMSIPGSKSLTNRHLIIAAIASGETTIHNLLESRDTNLMIEGL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHH
RRIGCKIEKLNHTGTHDTGVISPHCTCLNDLIQPSDVRIIPSKHYTCSTKIDCGLAGTVM
HHHCCEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCEEEECCCCCCHHHHHH
RFLPVLAGLCKGSVEFFGDDQAIRRPMDGTLHALRKLGVQVDGDRIPFTVHGRGEIEGGA
HHHHHHHHHHCCCCEEECCCHHHHCCCCHHHHHHHHCCCEECCCCCCEEEECCCCCCCCE
LETTEHSSSQFISGLLLSACRFKNGLTLKHIGNPLPSRPYIDMTVEVMREWGINVTHSDG
EECCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCEEEECCC
VWAVTPKELTGKHITIEPDLSNAAPFMIGAIVTGGSATIQNWPSKTSQPGKYLEAILPQF
EEEECHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHHHHHHHHH
GAEITKTANTITVSGTGNITGIRADLGHIGELVPNLVALATLAETPSVFYNIGHIRYHET
CCHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECEEEECCH
DRIEALVNEISSLGGTITAGKDYIKITPTTLTRSGVWKTYKDHRMATSGAIIGLRHKLTI
HHHHHHHHHHHHCCCEEECCCCEEEEECCEEECCCCCHHHHHCCEECCCCEEEEEEEEEE
EDIECTSKTFPRFADLWSGAFGK
ECCCCCHHCCHHHHHHHCCCCCC
>Mature Secondary Structure 
AKTEMYQPPLGNRALFKMSIPGSKSLTNRHLIIAAIASGETTIHNLLESRDTNLMIEGL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHH
RRIGCKIEKLNHTGTHDTGVISPHCTCLNDLIQPSDVRIIPSKHYTCSTKIDCGLAGTVM
HHHCCEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCEEEECCCCCCHHHHHH
RFLPVLAGLCKGSVEFFGDDQAIRRPMDGTLHALRKLGVQVDGDRIPFTVHGRGEIEGGA
HHHHHHHHHHCCCCEEECCCHHHHCCCCHHHHHHHHCCCEECCCCCCEEEECCCCCCCCE
LETTEHSSSQFISGLLLSACRFKNGLTLKHIGNPLPSRPYIDMTVEVMREWGINVTHSDG
EECCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCEEEECCC
VWAVTPKELTGKHITIEPDLSNAAPFMIGAIVTGGSATIQNWPSKTSQPGKYLEAILPQF
EEEECHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHHHHHHHHH
GAEITKTANTITVSGTGNITGIRADLGHIGELVPNLVALATLAETPSVFYNIGHIRYHET
CCHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECEEEECCH
DRIEALVNEISSLGGTITAGKDYIKITPTTLTRSGVWKTYKDHRMATSGAIIGLRHKLTI
HHHHHHHHHHHHCCCEEECCCCEEEEECCEEECCCCCHHHHHCCEECCCCEEEEEEEEEE
EDIECTSKTFPRFADLWSGAFGK
ECCCCCHHCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174