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The map label for this gene is hemH

Identifier: 28572284

GI number: 28572284

Start: 126672

End: 127589

Strand: Reverse

Name: hemH

Synonym: TW119

Alternate gene names: 28572284

Gene position: 127589-126672 (Counterclockwise)

Preceding gene: 28572295

Following gene: 28572283

Centisome position: 13.78

GC content: 43.9

Gene sequence:

>918_bases
ATGACAAGAAAACTTGAAATACCAGAATCGCGCGACCTGCCAGATTGGTCTCATATAGGCGGAGGAAAGGTCCGTGAGCT
TTATGTCCATAGACAATACAAGAACATCCTCCTAATGTTTGCATCAAACCGGGTAAGCGCATTTGACTTTGTCTTAGAGC
CCGAAATTCCCGAAAAGGGGCGCATGCTGACACAAATGAGCAATTGGTGGTTCCGTAAACTGCCTGCCGAAAATCATCTT
CTGGAAAATTACGACCAAGAATTATATAGAGACCTTGCAAGCCACATTCCAAGCCACATTCTAGAAAGGTCAACAATATG
CAGGAAAATGGATATCATCCCATTTGAATTCGTTATAAGGGGTTACTTAACTGGCTCAGCATGGGCAGATTATCTCGAAA
ACAACACGGTTTACGGGATCAAGCTAAAGGGAAAATTTCGCCAGGGTGATAGGCTCCCCGGGCCAATTTTTACCCCAACG
ACAAAAAGTAGAACAAAGGATACGCCCGTTCGCTATGAAGATCTTGTAAATGCAATCGGTTTGAGCCATGCGCAGCAACT
TAGAGAGATGTGCACGGACTATTACACAACCGCTGAACAGATAGCACGCAACAAGGGTCTTATTATTGCAGACGCAAAGT
TTGAGTTTGGCATAGCTGAAGGGAAAGCGTATCTTGCTGACGAGCTGCTAACAGCTGATTCGGCAAGATATTGGGATATC
AATTCATGGGGGCAGTTTGATTTGCCCATAGAACGCAGATTGGATAGCTTTGACAAGCAAGCTGTGCGCGATTGGGTAAA
GTGCAATTCTGGCAAAAACATAACCCCACAAAACATAACCCCACTGAGACTTCCACAGGAACTTATTCAAACTGTTTATA
AAAAGTACAAAACATTACTAGCTAAGCTAACGGGATGA

Upstream 100 bases:

>100_bases
CTCGTGCATATCATAAGATGACCCCAGTGGTCATAAATCGTATCGAGAGTTAGCATGAATCGTAATATGTTAGCGAATCC
CACCATTAGACGGATTACAA

Downstream 100 bases:

>100_bases
AAAAGGGGCTGATGAAGAAGGGGCAAAGGTCTGGTCACCAATAAAAACCCTAATCTGCCTGATCCCTGAAGAACTGTCTT
CTGATAACTCCTCACTGCGC

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTRKLEIPESRDLPDWSHIGGGKVRELYVHRQYKNILLMFASNRVSAFDFVLEPEIPEKGRMLTQMSNWWFRKLPAENHL
LENYDQELYRDLASHIPSHILERSTICRKMDIIPFEFVIRGYLTGSAWADYLENNTVYGIKLKGKFRQGDRLPGPIFTPT
TKSRTKDTPVRYEDLVNAIGLSHAQQLREMCTDYYTTAEQIARNKGLIIADAKFEFGIAEGKAYLADELLTADSARYWDI
NSWGQFDLPIERRLDSFDKQAVRDWVKCNSGKNITPQNITPLRLPQELIQTVYKKYKTLLAKLTG

Sequences:

>Translated_305_residues
MTRKLEIPESRDLPDWSHIGGGKVRELYVHRQYKNILLMFASNRVSAFDFVLEPEIPEKGRMLTQMSNWWFRKLPAENHL
LENYDQELYRDLASHIPSHILERSTICRKMDIIPFEFVIRGYLTGSAWADYLENNTVYGIKLKGKFRQGDRLPGPIFTPT
TKSRTKDTPVRYEDLVNAIGLSHAQQLREMCTDYYTTAEQIARNKGLIIADAKFEFGIAEGKAYLADELLTADSARYWDI
NSWGQFDLPIERRLDSFDKQAVRDWVKCNSGKNITPQNITPLRLPQELIQTVYKKYKTLLAKLTG
>Mature_304_residues
TRKLEIPESRDLPDWSHIGGGKVRELYVHRQYKNILLMFASNRVSAFDFVLEPEIPEKGRMLTQMSNWWFRKLPAENHLL
ENYDQELYRDLASHIPSHILERSTICRKMDIIPFEFVIRGYLTGSAWADYLENNTVYGIKLKGKFRQGDRLPGPIFTPTT
KSRTKDTPVRYEDLVNAIGLSHAQQLREMCTDYYTTAEQIARNKGLIIADAKFEFGIAEGKAYLADELLTADSARYWDIN
SWGQFDLPIERRLDSFDKQAVRDWVKCNSGKNITPQNITPLRLPQELIQTVYKKYKTLLAKLTG

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6319326, Length=305, Percent_Identity=33.7704918032787, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_TROW8 (Q83IB9)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789064.1
- ProteinModelPortal:   Q83IB9
- STRING:   Q83IB9
- GeneID:   1064416
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW119
- eggNOG:   COG0152
- HOGENOM:   HBG555040
- OMA:   LTLMDEV
- PhylomeDB:   Q83IB9
- ProtClustDB:   PRK13961
- BioCyc:   TWHI218496:TW0109-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 35415; Mature: 35284

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKLEIPESRDLPDWSHIGGGKVRELYVHRQYKNILLMFASNRVSAFDFVLEPEIPEKG
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCH
RMLTQMSNWWFRKLPAENHLLENYDQELYRDLASHIPSHILERSTICRKMDIIPFEFVIR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GYLTGSAWADYLENNTVYGIKLKGKFRQGDRLPGPIFTPTTKSRTKDTPVRYEDLVNAIG
HHHCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LSHAQQLREMCTDYYTTAEQIARNKGLIIADAKFEFGIAEGKAYLADELLTADSARYWDI
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHCCCCCEEEC
NSWGQFDLPIERRLDSFDKQAVRDWVKCNSGKNITPQNITPLRLPQELIQTVYKKYKTLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AKLTG
HHHCC
>Mature Secondary Structure 
TRKLEIPESRDLPDWSHIGGGKVRELYVHRQYKNILLMFASNRVSAFDFVLEPEIPEKG
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCH
RMLTQMSNWWFRKLPAENHLLENYDQELYRDLASHIPSHILERSTICRKMDIIPFEFVIR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GYLTGSAWADYLENNTVYGIKLKGKFRQGDRLPGPIFTPTTKSRTKDTPVRYEDLVNAIG
HHHCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LSHAQQLREMCTDYYTTAEQIARNKGLIIADAKFEFGIAEGKAYLADELLTADSARYWDI
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHCCCCCEEEC
NSWGQFDLPIERRLDSFDKQAVRDWVKCNSGKNITPQNITPLRLPQELIQTVYKKYKTLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AKLTG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174