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The map label for this gene is guaA

Identifier: 28572257

GI number: 28572257

Start: 87199

End: 88710

Strand: Direct

Name: guaA

Synonym: TW089

Alternate gene names: 28572257

Gene position: 87199-88710 (Clockwise)

Preceding gene: 28572256

Following gene: 28572258

Centisome position: 9.42

GC content: 46.36

Gene sequence:

>1512_bases
ATGAGACCTGTTCTTGTCGTTGATTTTGGCTCTCAATATTCGCAGCTTGTAGTCCGTGCGATAAGAGAGTGCGGGTATTA
TGCAGAGTTTGCGTCCCCGAGTATTTCTGCTGCTGAATGTCTTGCCTTGTCCCCGATTGCCATATTTCTCTCTGGAGGGC
CTGCTAGTGCATACAAAGATAATGCCCCCAAGCTTGACGAAGAGATTTTTAATTGTGGCATTCCCATTTTTGGCATTTGC
TACGGCTTTCAACTGCTTGCACAGGCATTCGGTGGATCTGTCAAAAAAGCAAATGCTCCGGAATACGGCCCTGCGGATAT
AACAATTGTCAACAAGGCGTTTTTTTCTGGCCAACCCGACCGCCAGACAGTGTGGATGAGCCATGGTGATAGTGTTATAC
GCGCTCCCAAAAATTTTTGCATTCTTTCAACTTCTCAGGATGCTGTTCTTTCATTTTGCAACCGTGATAGAACAATTGCT
GGCGTGCAATGGCACCCGGAAGTAAAGCACAGCCGGTTTGGTAAACACACAATAAAAGCCTTTTTATCAAGTTTTGCCGC
GCCAAACTGGGATCCAGAACAGACAATTTGTGGGACAGTTGACAGTATCCGCAAAACTGTTGGTTGTAAGAGGGTTTTGT
GTGCTCTTAGCGGAGGAGTTGACTCTGTCGTTGCCGCAACACTCACACACCGTGCGATAGGCGATAGGCTGCGATGTGTT
TTTGTTGATCATGGTCTTCTTCGGTTGAATGAGAGGGAGCAGGTTGAGGAGTATTGTTCATCTCTTGGTCTTAATGTGTC
TACTTATGATGCCTCAGATTGTTTTTTGAGCGCCCTTTCAGGGATACGTGATTCTGAGCAAAAGCGTAAGGTAATTGGTC
GCGAGTTTATTGCCTGTTTTTCAAAATTGCAAGAGCGATTTGATATAAAGCCGCACTTTCTCTTACAGGGCACGCTTTAT
CCGGATCTCGTTGAATCCGGAGCAACCCCGGGTGGCGCGACTATAAAAAGTCATCATAATGTTGGTGGCTTGTCGGATAA
TCTTGGTTTTGAATTATTAGAACCGCTGAAGTATCTTTTCAAGGATGAAGTGCGAAAAATTGGCCTTCAACTCGGTATAC
CTAAGCACATAGTGCACAGACAGCCCTTCCCTGGCCCTGGCCTTGCTATAAGAATAATTGGTGAGGTTACAAACAAGAAG
CTGTCTATTCTGCGTGCGGCCGATGCAATTGTCAGGCATGAGTTGCGAGACTGGACTGATATCTGGCAGTGCCCAGTTAT
CTTACTATCTGACGTGCAATCAGTAGGCGTTCGCGGAGATAGTAGATCATGCGGCTTTCCGATTGTTATCCGGCCCGTTT
CGTCTGATGATGCAATGACCGCGGATTGGTACCGATTACCATACGATGTTCTTGCACGAATTTCTGGTCGTATAACAAAC
GAAATTCCTGAAATCGTGCGTGTTGTTCTTGATATCACACCCAAGCCCCCAGCAACGATAGAGTGGGAGTAG

Upstream 100 bases:

>100_bases
CACTATTTATCGTTATCGCTCTCGGCGGAGTTGTGCCTCTTATGAGTTTTGTGGTCGAGCGGTATGTTTACAAGAAACTT
TCAAAACGGTTTGTGTCGAC

Downstream 100 bases:

>100_bases
GGTATCTTACTGCTTGGACTTTGCGTCGATGCACTACTGAGCTTTGAGGCTACCGGGTTTACGGGGTATTCTAGACGCTG
TGCGCTCTAGAGAGCGCAGC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRPVLVVDFGSQYSQLVVRAIRECGYYAEFASPSISAAECLALSPIAIFLSGGPASAYKDNAPKLDEEIFNCGIPIFGIC
YGFQLLAQAFGGSVKKANAPEYGPADITIVNKAFFSGQPDRQTVWMSHGDSVIRAPKNFCILSTSQDAVLSFCNRDRTIA
GVQWHPEVKHSRFGKHTIKAFLSSFAAPNWDPEQTICGTVDSIRKTVGCKRVLCALSGGVDSVVAATLTHRAIGDRLRCV
FVDHGLLRLNEREQVEEYCSSLGLNVSTYDASDCFLSALSGIRDSEQKRKVIGREFIACFSKLQERFDIKPHFLLQGTLY
PDLVESGATPGGATIKSHHNVGGLSDNLGFELLEPLKYLFKDEVRKIGLQLGIPKHIVHRQPFPGPGLAIRIIGEVTNKK
LSILRAADAIVRHELRDWTDIWQCPVILLSDVQSVGVRGDSRSCGFPIVIRPVSSDDAMTADWYRLPYDVLARISGRITN
EIPEIVRVVLDITPKPPATIEWE

Sequences:

>Translated_503_residues
MRPVLVVDFGSQYSQLVVRAIRECGYYAEFASPSISAAECLALSPIAIFLSGGPASAYKDNAPKLDEEIFNCGIPIFGIC
YGFQLLAQAFGGSVKKANAPEYGPADITIVNKAFFSGQPDRQTVWMSHGDSVIRAPKNFCILSTSQDAVLSFCNRDRTIA
GVQWHPEVKHSRFGKHTIKAFLSSFAAPNWDPEQTICGTVDSIRKTVGCKRVLCALSGGVDSVVAATLTHRAIGDRLRCV
FVDHGLLRLNEREQVEEYCSSLGLNVSTYDASDCFLSALSGIRDSEQKRKVIGREFIACFSKLQERFDIKPHFLLQGTLY
PDLVESGATPGGATIKSHHNVGGLSDNLGFELLEPLKYLFKDEVRKIGLQLGIPKHIVHRQPFPGPGLAIRIIGEVTNKK
LSILRAADAIVRHELRDWTDIWQCPVILLSDVQSVGVRGDSRSCGFPIVIRPVSSDDAMTADWYRLPYDVLARISGRITN
EIPEIVRVVLDITPKPPATIEWE
>Mature_503_residues
MRPVLVVDFGSQYSQLVVRAIRECGYYAEFASPSISAAECLALSPIAIFLSGGPASAYKDNAPKLDEEIFNCGIPIFGIC
YGFQLLAQAFGGSVKKANAPEYGPADITIVNKAFFSGQPDRQTVWMSHGDSVIRAPKNFCILSTSQDAVLSFCNRDRTIA
GVQWHPEVKHSRFGKHTIKAFLSSFAAPNWDPEQTICGTVDSIRKTVGCKRVLCALSGGVDSVVAATLTHRAIGDRLRCV
FVDHGLLRLNEREQVEEYCSSLGLNVSTYDASDCFLSALSGIRDSEQKRKVIGREFIACFSKLQERFDIKPHFLLQGTLY
PDLVESGATPGGATIKSHHNVGGLSDNLGFELLEPLKYLFKDEVRKIGLQLGIPKHIVHRQPFPGPGLAIRIIGEVTNKK
LSILRAADAIVRHELRDWTDIWQCPVILLSDVQSVGVRGDSRSCGFPIVIRPVSSDDAMTADWYRLPYDVLARISGRITN
EIPEIVRVVLDITPKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=555, Percent_Identity=32.4324324324324, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1788854, Length=518, Percent_Identity=46.1389961389961, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71992717, Length=419, Percent_Identity=38.1861575178998, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71992710, Length=419, Percent_Identity=38.1861575178998, Blast_Score=260, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI133901714, Length=419, Percent_Identity=38.1861575178998, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6323873, Length=517, Percent_Identity=45.2611218568665, Blast_Score=426, Evalue=1e-120,
Organism=Drosophila melanogaster, GI281365319, Length=538, Percent_Identity=31.7843866171004, Blast_Score=236, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_TROW8 (Q83ID3)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789037.1
- ProteinModelPortal:   Q83ID3
- SMR:   Q83ID3
- STRING:   Q83ID3
- GeneID:   1064337
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW089
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   PSTIEPC
- ProtClustDB:   PRK00074
- BioCyc:   TWHI218496:TW0082-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 55377; Mature: 55377

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 165-165 ACT_SITE 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVLVVDFGSQYSQLVVRAIRECGYYAEFASPSISAAECLALSPIAIFLSGGPASAYKD
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHCCEEEEEECCCCCCCCC
NAPKLDEEIFNCGIPIFGICYGFQLLAQAFGGSVKKANAPEYGPADITIVNKAFFSGQPD
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHCCCCC
RQTVWMSHGDSVIRAPKNFCILSTSQDAVLSFCNRDRTIAGVQWHPEVKHSRFGKHTIKA
CCEEEECCCCHHEECCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHH
FLSSFAAPNWDPEQTICGTVDSIRKTVGCKRVLCALSGGVDSVVAATLTHRAIGDRLRCV
HHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEE
FVDHGLLRLNEREQVEEYCSSLGLNVSTYDASDCFLSALSGIRDSEQKRKVIGREFIACF
EECCCHHCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SKLQERFDIKPHFLLQGTLYPDLVESGATPGGATIKSHHNVGGLSDNLGFELLEPLKYLF
HHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
KDEVRKIGLQLGIPKHIVHRQPFPGPGLAIRIIGEVTNKKLSILRAADAIVRHELRDWTD
HHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHH
IWQCPVILLSDVQSVGVRGDSRSCGFPIVIRPVSSDDAMTADWYRLPYDVLARISGRITN
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHH
EIPEIVRVVLDITPKPPATIEWE
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRPVLVVDFGSQYSQLVVRAIRECGYYAEFASPSISAAECLALSPIAIFLSGGPASAYKD
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHCCEEEEEECCCCCCCCC
NAPKLDEEIFNCGIPIFGICYGFQLLAQAFGGSVKKANAPEYGPADITIVNKAFFSGQPD
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHCCCCC
RQTVWMSHGDSVIRAPKNFCILSTSQDAVLSFCNRDRTIAGVQWHPEVKHSRFGKHTIKA
CCEEEECCCCHHEECCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHH
FLSSFAAPNWDPEQTICGTVDSIRKTVGCKRVLCALSGGVDSVVAATLTHRAIGDRLRCV
HHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEE
FVDHGLLRLNEREQVEEYCSSLGLNVSTYDASDCFLSALSGIRDSEQKRKVIGREFIACF
EECCCHHCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SKLQERFDIKPHFLLQGTLYPDLVESGATPGGATIKSHHNVGGLSDNLGFELLEPLKYLF
HHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
KDEVRKIGLQLGIPKHIVHRQPFPGPGLAIRIIGEVTNKKLSILRAADAIVRHELRDWTD
HHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHH
IWQCPVILLSDVQSVGVRGDSRSCGFPIVIRPVSSDDAMTADWYRLPYDVLARISGRITN
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHH
EIPEIVRVVLDITPKPPATIEWE
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174