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The map label for this gene is pcrA [H]

Identifier: 28572258

GI number: 28572258

Start: 88815

End: 91046

Strand: Direct

Name: pcrA [H]

Synonym: TW090

Alternate gene names: 28572258

Gene position: 88815-91046 (Clockwise)

Preceding gene: 28572257

Following gene: 28572259

Centisome position: 9.59

GC content: 44.8

Gene sequence:

>2232_bases
ATGACAATTCCGGCCGATTTACTCAAGGGTTTGAACCCCCAGCAGGTGGAGGGTGTCTGCTATAGGGGGCCAACCCTTCT
TATTTCCGCTGGCGCCGGCAGTGGTAAGACTAAGGTGCTGACCCATAGGATTGCCGGATTTCTGGCAACAGGTGAAGCAT
CCTGTGACCAGATATTGGCGATTACATTCACAAATAAGGCTGCATCTGAAATGCGCCATCGCACCGGGGATCTTGTTGGA
TTATCCGTCAGAGATATGCAGATATCTACCTTCCATTCAGTCTGTGTGAGAATTCTTCGTGAATGTGTCGGGCATCTAGG
CAAGGCAAAGAATTTCACGATATATGATGCTGCAGACCAGCGTGCGATTATCAAACAAATCTGTCAGTCTACGGATACTT
TTGGACTAACGGCAAGCATGGTTGCCTCGCAAATAGGTCTGTTGAAAAACCAAATGCATACCCCAGACACCTATTCGCGC
TGTCTTGCCTCAAATTCCACTAATGGCAATTCGGTAACTTCGATTACCTTACAGAGAGAACAGTTTATCCTGCACGTTTT
TAGGCGCTACCAGGAGATTCTGAAAGAGGCAAATGCCTTTGACTTTGATGATCTTATTTCCGAAACAGTTTTTTTACTCC
AGAAATCCCCGGAGATTTCTGCAATGTATAGAAACCGCTGGAAGTATCTTTTGGTAGACGAATATCAGGATACAAACCAT
GCCCAGTATAGGCTTATTCTTGAGCTGACAAAAGGTGATAAAAGCGACATGTCTCTGACAGCTGTCGGTGATTCAGATCA
GAGTATATACGCTTTCCGGGGTGCTGATATTGCCAATATAGTGAATTTCGAAAACGATTTTCCGGACTGTCACACAATCT
TATTGGAGCAGAATTACCGCTCTTGCCAGAATATTCTTTTAGCTGCCAATGCGCTTATTTCAAATAATCAGAATCGTAAG
GATAAAAACCTGTGGAGCGAACTAGGTGCCGGTGAGAAGGTTGTTGCATACTGTGCATCTTCCGCAGAAGAAGAGGGGCG
TTTTGTTGCAGATAGGGTGCGCCATCTGGTCGATCAAGGTGAGAGCTTGTCTGATATAGCGGTTTTTTACCGATCCAATA
CTCAGAGCAGAGCAATAGAAGAATCACTCATCAGGCAATCAATTCCATACAGAGTTGTTGGCGGGACAAAATTTTATGAA
CGCGCTGAAATAAAAGATATTCTGGCGTATCTGACAGTAATTGTCAATCCAGACGATACGCTGGCTCTCAGGCGTATTCT
AAATGTTCCAAAGCGTGGTGTCGGAACGGTATCAGAGAATAGGCTTGTTGAATTTGCCAGTGCTAGAGGCATAACACTTA
TGCAGGCACTTTCTCGCATTGATGAAATTAACATTCAGCCATCTGCCAAATCAGCTCTTCGGGATCTCTTTTACTTATTA
AATCGCTTTGCCGGATCTAGTATGCCGGCCAATGAACTGCTCGACGACCTGCTAAAAGCTACAGACCTTCTAAGTCGATT
CAACAACACTGATGATCCTCAAAATGAGGCTAGAAAGATCAATATTGGGGACTTTGTAGATTCGGTTGAGGATTTCGCAA
AAGAAAACCCCGGAGGCACTCTATCTGACTTTATGACAAATATTGCACTTATGACATCCTACGATCAGTCTGATGACGCA
CCGGGCCAGATTACCCTCATGACCTTACATATGGCAAAGGGTCTTGAATTTGATTTTGTGTTTCTAACTGGTCTGGAAGA
GGGTATCCTTCCTCACGAAATGTCTCTTAAAGAGAAGAATGGCATAGAAGAAGAGCGTAGACTTGCCTATGTTGGTATGA
CGCGCGCAAAAAAAGTTCTTCACCTTTCTTTTAGTAGCTACCGAAAACGATACACAGATGGGGTGCGCTTGCCGAGTCGT
TTTTGGGATGAAATTCCTCAGCATCTCCTGCGGTGGCACAGTCGGTTTACACACAAGAGATTTACCGGTCCCCTGTACGG
GTTTACAGCGCGTCCCGCAGGGGAGTCACGGCAATCAGTGCATGTTTTGAAAGACCGATACCCCCGTGAGAGTTTTTCTA
TTTCGGATAGTGTAAAGCATTCCGTTTTTGGTGTTGGAACGGTGGTTGATGTTCTTGGTTTGGGCCCAAAACAGATAATT
GTTGTTGAATTTGCTGATAAACGCCGCAGTTTGCTCGCGAGTATTGCACCCATTGTAAAATGCAAACAATAG

Upstream 100 bases:

>100_bases
TCTTACTGCTTGGACTTTGCGTCGATGCACTACTGAGCTTTGAGGCTACCGGGTTTACGGGGTATTCTAGACGCTGTGCG
CTCTAGAGAGCGCAGCACTT

Downstream 100 bases:

>100_bases
AATCAATAATTCTCCGTACAGTTGCATGGTGCGAATGGTCCAGATCGAAGTGAGATTTCTGCGCTACGCGGCAGTGTTCT
TGTTAAGGATTTTCAGCCTG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MTIPADLLKGLNPQQVEGVCYRGPTLLISAGAGSGKTKVLTHRIAGFLATGEASCDQILAITFTNKAASEMRHRTGDLVG
LSVRDMQISTFHSVCVRILRECVGHLGKAKNFTIYDAADQRAIIKQICQSTDTFGLTASMVASQIGLLKNQMHTPDTYSR
CLASNSTNGNSVTSITLQREQFILHVFRRYQEILKEANAFDFDDLISETVFLLQKSPEISAMYRNRWKYLLVDEYQDTNH
AQYRLILELTKGDKSDMSLTAVGDSDQSIYAFRGADIANIVNFENDFPDCHTILLEQNYRSCQNILLAANALISNNQNRK
DKNLWSELGAGEKVVAYCASSAEEEGRFVADRVRHLVDQGESLSDIAVFYRSNTQSRAIEESLIRQSIPYRVVGGTKFYE
RAEIKDILAYLTVIVNPDDTLALRRILNVPKRGVGTVSENRLVEFASARGITLMQALSRIDEINIQPSAKSALRDLFYLL
NRFAGSSMPANELLDDLLKATDLLSRFNNTDDPQNEARKINIGDFVDSVEDFAKENPGGTLSDFMTNIALMTSYDQSDDA
PGQITLMTLHMAKGLEFDFVFLTGLEEGILPHEMSLKEKNGIEEERRLAYVGMTRAKKVLHLSFSSYRKRYTDGVRLPSR
FWDEIPQHLLRWHSRFTHKRFTGPLYGFTARPAGESRQSVHVLKDRYPRESFSISDSVKHSVFGVGTVVDVLGLGPKQII
VVEFADKRRSLLASIAPIVKCKQ

Sequences:

>Translated_743_residues
MTIPADLLKGLNPQQVEGVCYRGPTLLISAGAGSGKTKVLTHRIAGFLATGEASCDQILAITFTNKAASEMRHRTGDLVG
LSVRDMQISTFHSVCVRILRECVGHLGKAKNFTIYDAADQRAIIKQICQSTDTFGLTASMVASQIGLLKNQMHTPDTYSR
CLASNSTNGNSVTSITLQREQFILHVFRRYQEILKEANAFDFDDLISETVFLLQKSPEISAMYRNRWKYLLVDEYQDTNH
AQYRLILELTKGDKSDMSLTAVGDSDQSIYAFRGADIANIVNFENDFPDCHTILLEQNYRSCQNILLAANALISNNQNRK
DKNLWSELGAGEKVVAYCASSAEEEGRFVADRVRHLVDQGESLSDIAVFYRSNTQSRAIEESLIRQSIPYRVVGGTKFYE
RAEIKDILAYLTVIVNPDDTLALRRILNVPKRGVGTVSENRLVEFASARGITLMQALSRIDEINIQPSAKSALRDLFYLL
NRFAGSSMPANELLDDLLKATDLLSRFNNTDDPQNEARKINIGDFVDSVEDFAKENPGGTLSDFMTNIALMTSYDQSDDA
PGQITLMTLHMAKGLEFDFVFLTGLEEGILPHEMSLKEKNGIEEERRLAYVGMTRAKKVLHLSFSSYRKRYTDGVRLPSR
FWDEIPQHLLRWHSRFTHKRFTGPLYGFTARPAGESRQSVHVLKDRYPRESFSISDSVKHSVFGVGTVVDVLGLGPKQII
VVEFADKRRSLLASIAPIVKCKQ
>Mature_742_residues
TIPADLLKGLNPQQVEGVCYRGPTLLISAGAGSGKTKVLTHRIAGFLATGEASCDQILAITFTNKAASEMRHRTGDLVGL
SVRDMQISTFHSVCVRILRECVGHLGKAKNFTIYDAADQRAIIKQICQSTDTFGLTASMVASQIGLLKNQMHTPDTYSRC
LASNSTNGNSVTSITLQREQFILHVFRRYQEILKEANAFDFDDLISETVFLLQKSPEISAMYRNRWKYLLVDEYQDTNHA
QYRLILELTKGDKSDMSLTAVGDSDQSIYAFRGADIANIVNFENDFPDCHTILLEQNYRSCQNILLAANALISNNQNRKD
KNLWSELGAGEKVVAYCASSAEEEGRFVADRVRHLVDQGESLSDIAVFYRSNTQSRAIEESLIRQSIPYRVVGGTKFYER
AEIKDILAYLTVIVNPDDTLALRRILNVPKRGVGTVSENRLVEFASARGITLMQALSRIDEINIQPSAKSALRDLFYLLN
RFAGSSMPANELLDDLLKATDLLSRFNNTDDPQNEARKINIGDFVDSVEDFAKENPGGTLSDFMTNIALMTSYDQSDDAP
GQITLMTLHMAKGLEFDFVFLTGLEEGILPHEMSLKEKNGIEEERRLAYVGMTRAKKVLHLSFSSYRKRYTDGVRLPSRF
WDEIPQHLLRWHSRFTHKRFTGPLYGFTARPAGESRQSVHVLKDRYPRESFSISDSVKHSVFGVGTVVDVLGLGPKQIIV
VEFADKRRSLLASIAPIVKCKQ

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=728, Percent_Identity=37.0879120879121, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI48994965, Length=662, Percent_Identity=37.7643504531722, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1787196, Length=353, Percent_Identity=29.7450424929178, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6322369, Length=772, Percent_Identity=27.5906735751295, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=669, Percent_Identity=22.7204783258595, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83256; Mature: 83125

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPADLLKGLNPQQVEGVCYRGPTLLISAGAGSGKTKVLTHRIAGFLATGEASCDQILA
CCCCHHHHCCCCHHHHCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEE
ITFTNKAASEMRHRTGDLVGLSVRDMQISTFHSVCVRILRECVGHLGKAKNFTIYDAADQ
EEECCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
RAIIKQICQSTDTFGLTASMVASQIGLLKNQMHTPDTYSRCLASNSTNGNSVTSITLQRE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEEHHH
QFILHVFRRYQEILKEANAFDFDDLISETVFLLQKSPEISAMYRNRWKYLLVDEYQDTNH
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCC
AQYRLILELTKGDKSDMSLTAVGDSDQSIYAFRGADIANIVNFENDFPDCHTILLEQNYR
CEEEEEEEEECCCCCCCEEEEECCCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHH
SCQNILLAANALISNNQNRKDKNLWSELGAGEKVVAYCASSAEEEGRFVADRVRHLVDQG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHHHHHHHHHHCC
ESLSDIAVFYRSNTQSRAIEESLIRQSIPYRVVGGTKFYERAEIKDILAYLTVIVNPDDT
CCHHHHEEEEECCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHEEECCCHH
LALRRILNVPKRGVGTVSENRLVEFASARGITLMQALSRIDEINIQPSAKSALRDLFYLL
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
NRFAGSSMPANELLDDLLKATDLLSRFNNTDDPQNEARKINIGDFVDSVEDFAKENPGGT
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCEEEEHHHHHHHHHHHHHCCCCCC
LSDFMTNIALMTSYDQSDDAPGQITLMTLHMAKGLEFDFVFLTGLEEGILPHEMSLKEKN
HHHHHHHHHHEECCCCCCCCCCEEEEEEEEHHCCCCEEEEEEECCCCCCCCCCCCCHHHC
GIEEERRLAYVGMTRAKKVLHLSFSSYRKRYTDGVRLPSRFWDEIPQHLLRWHSRFTHKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FTGPLYGFTARPAGESRQSVHVLKDRYPRESFSISDSVKHSVFGVGTVVDVLGLGPKQII
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
VVEFADKRRSLLASIAPIVKCKQ
EEEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIPADLLKGLNPQQVEGVCYRGPTLLISAGAGSGKTKVLTHRIAGFLATGEASCDQILA
CCCHHHHCCCCHHHHCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEE
ITFTNKAASEMRHRTGDLVGLSVRDMQISTFHSVCVRILRECVGHLGKAKNFTIYDAADQ
EEECCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
RAIIKQICQSTDTFGLTASMVASQIGLLKNQMHTPDTYSRCLASNSTNGNSVTSITLQRE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEEHHH
QFILHVFRRYQEILKEANAFDFDDLISETVFLLQKSPEISAMYRNRWKYLLVDEYQDTNH
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCC
AQYRLILELTKGDKSDMSLTAVGDSDQSIYAFRGADIANIVNFENDFPDCHTILLEQNYR
CEEEEEEEEECCCCCCCEEEEECCCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHH
SCQNILLAANALISNNQNRKDKNLWSELGAGEKVVAYCASSAEEEGRFVADRVRHLVDQG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHHHHHHHHHHCC
ESLSDIAVFYRSNTQSRAIEESLIRQSIPYRVVGGTKFYERAEIKDILAYLTVIVNPDDT
CCHHHHEEEEECCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHEEECCCHH
LALRRILNVPKRGVGTVSENRLVEFASARGITLMQALSRIDEINIQPSAKSALRDLFYLL
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
NRFAGSSMPANELLDDLLKATDLLSRFNNTDDPQNEARKINIGDFVDSVEDFAKENPGGT
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCEEEEHHHHHHHHHHHHHCCCCCC
LSDFMTNIALMTSYDQSDDAPGQITLMTLHMAKGLEFDFVFLTGLEEGILPHEMSLKEKN
HHHHHHHHHHEECCCCCCCCCCEEEEEEEEHHCCCCEEEEEEECCCCCCCCCCCCCHHHC
GIEEERRLAYVGMTRAKKVLHLSFSSYRKRYTDGVRLPSRFWDEIPQHLLRWHSRFTHKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FTGPLYGFTARPAGESRQSVHVLKDRYPRESFSISDSVKHSVFGVGTVVDVLGLGPKQII
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
VVEFADKRRSLLASIAPIVKCKQ
EEEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]