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The map label for this gene is ubiE

Identifier: 28572233

GI number: 28572233

Start: 53375

End: 54094

Strand: Direct

Name: ubiE

Synonym: TW063

Alternate gene names: 28572233

Gene position: 53375-54094 (Clockwise)

Preceding gene: 28572231

Following gene: 28572234

Centisome position: 5.76

GC content: 49.31

Gene sequence:

>720_bases
TTGGGGACTAACATTCACATTGATATGGTAATTCACGGCAAACACACGGCAGAGATTAGAAAAATGTTCAATAGGGTTGC
CCAAGCCTACGATAGAACAAATCTTGTCTTGTCATTCTTACAGGATGCCCACTGGCGTCGGGCTGCTTGTAAGATGCTTG
GGGTAACTGCCGGGGAAGAGGTGCTTGATGTCGGTGCTGGTACTGGCGCGAGCACCCGAACAGTTGCCAGGACCGGTGCT
GCCGTCACAGGAATTGATATATCTCCCCGCATGCTCCAAATTGCACGCAATCGATGTAAAAGGTTTCAAAACATCACGTG
GCGTCTCACCAATGGCGATCTGCCGTTCCCTGATAAGAGCTTCGACGCTATTCTAATGGTATTTTGTCTCAGAAATGTGT
CCAATATTCAGGGATTCTTATGCGATGCTGCACGCGTCTTGAAGCCCGGTGGGCGGCTTGTAGTGTGCGAGTTCAGCCAT
CCCAGAAGGTTTGTTGCCCCGTTCTACAGGTTGTATCTCAGGTATGTCTTGCCCAGACTTGCAAAACTTATCTCGAGCGA
TCCTGCTGCATACGAATATCTTACAGAATCAATCGAGGATTGGTACGAGGTTGACGAGCTTGCGTTTATGCTTGAACAGT
GTGGTTTTCAGAATACATCCTGGAAAAGGTTAAGCTTTGGGGCAGTTGCCTTGCATAGGGCGCTGCGTGGTCCGGAGTAG

Upstream 100 bases:

>100_bases
AGCATGGTAACCCTCGTCGTAACTCGGTTCAAGTATTTTGTTATTTAGAAAATAAACCGATGTGTAAAGTTTTTGCACCT
GGGACTATACTTTCTTCGCT

Downstream 100 bases:

>100_bases
CAAACCCGTACATGCTTGTGTCAGGTATTTTTCTGTCCGATATTCTTGATCCATCTGGCAGTCTGAGTGATGATATTTCT
CCCGCTCTCGATAGGGTTGA

Product: ubiquinone/menaquinone methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MGTNIHIDMVIHGKHTAEIRKMFNRVAQAYDRTNLVLSFLQDAHWRRAACKMLGVTAGEEVLDVGAGTGASTRTVARTGA
AVTGIDISPRMLQIARNRCKRFQNITWRLTNGDLPFPDKSFDAILMVFCLRNVSNIQGFLCDAARVLKPGGRLVVCEFSH
PRRFVAPFYRLYLRYVLPRLAKLISSDPAAYEYLTESIEDWYEVDELAFMLEQCGFQNTSWKRLSFGAVALHRALRGPE

Sequences:

>Translated_239_residues
MGTNIHIDMVIHGKHTAEIRKMFNRVAQAYDRTNLVLSFLQDAHWRRAACKMLGVTAGEEVLDVGAGTGASTRTVARTGA
AVTGIDISPRMLQIARNRCKRFQNITWRLTNGDLPFPDKSFDAILMVFCLRNVSNIQGFLCDAARVLKPGGRLVVCEFSH
PRRFVAPFYRLYLRYVLPRLAKLISSDPAAYEYLTESIEDWYEVDELAFMLEQCGFQNTSWKRLSFGAVALHRALRGPE
>Mature_238_residues
GTNIHIDMVIHGKHTAEIRKMFNRVAQAYDRTNLVLSFLQDAHWRRAACKMLGVTAGEEVLDVGAGTGASTRTVARTGAA
VTGIDISPRMLQIARNRCKRFQNITWRLTNGDLPFPDKSFDAILMVFCLRNVSNIQGFLCDAARVLKPGGRLVVCEFSHP
RRFVAPFYRLYLRYVLPRLAKLISSDPAAYEYLTESIEDWYEVDELAFMLEQCGFQNTSWKRLSFGAVALHRALRGPE

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=275, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI48994983, Length=240, Percent_Identity=37.9166666666667, Blast_Score=144, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17557039, Length=235, Percent_Identity=30.6382978723404, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323526, Length=237, Percent_Identity=28.6919831223629, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=31.3725490196078, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_TROW8 (P67065)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789013.1
- ProteinModelPortal:   P67065
- STRING:   P67065
- GeneID:   1064342
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW063
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   LGQHRIW
- PhylomeDB:   P67065
- ProtClustDB:   CLSK229445
- BioCyc:   TWHI218496:TW0058-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27046; Mature: 26914

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTNIHIDMVIHGKHTAEIRKMFNRVAQAYDRTNLVLSFLQDAHWRRAACKMLGVTAGEE
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VLDVGAGTGASTRTVARTGAAVTGIDISPRMLQIARNRCKRFQNITWRLTNGDLPFPDKS
HHHCCCCCCCCCHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCH
FDAILMVFCLRNVSNIQGFLCDAARVLKPGGRLVVCEFSHPRRFVAPFYRLYLRYVLPRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
AKLISSDPAAYEYLTESIEDWYEVDELAFMLEQCGFQNTSWKRLSFGAVALHRALRGPE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GTNIHIDMVIHGKHTAEIRKMFNRVAQAYDRTNLVLSFLQDAHWRRAACKMLGVTAGEE
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VLDVGAGTGASTRTVARTGAAVTGIDISPRMLQIARNRCKRFQNITWRLTNGDLPFPDKS
HHHCCCCCCCCCHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCH
FDAILMVFCLRNVSNIQGFLCDAARVLKPGGRLVVCEFSHPRRFVAPFYRLYLRYVLPRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
AKLISSDPAAYEYLTESIEDWYEVDELAFMLEQCGFQNTSWKRLSFGAVALHRALRGPE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174