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The map label for this gene is acbB [H]

Identifier: 28572207

GI number: 28572207

Start: 30664

End: 31647

Strand: Reverse

Name: acbB [H]

Synonym: TW033

Alternate gene names: 28572207

Gene position: 31647-30664 (Counterclockwise)

Preceding gene: 28572210

Following gene: 28572206

Centisome position: 3.42

GC content: 45.63

Gene sequence:

>984_bases
ATGACGCATATTCTTGTTACTGGCGGTGCTGGATTTATTGGCTCTAACTTTGTGCGCAGGGCAATTGAGAACAAATACCC
GGAGATTAGAGATTGTACAGTGACAGTGCTTGACGCGCTAACTTACTCGGGCAATATTAACAATTTATCTCCTGTCAGAT
CGTCGAGTCGCCTTCGATTTATTCATGGTGATATACGTGATGAAGATCTCCTGAGTGAGATCATGCCCAATGTGAACTAC
GTTGTGCATTTCGCTGCAGAAACACATGTTGATAGGTCAATTAAAAACGCATCAGCTTTTGTGGAAACTAATGTGCTTGG
TACAGGGCGGCTTCTGGAAGCTGCTGCCCGAAATAACATAGACCGGTTCATTCATATATCAACTGATGAGGTCTATGGTT
CGATTGAAAGTGGAAGCTGGGACGAAAATCAACCGCTTCTGCCAAACTCCCCGTACTCGGCATCAAAGGCGGCAAGTGAC
CTCTTGGTCAGGAGCTATCACAAAACCTACGACCTGCCGGTTTGCATTACGAGGTGCTCGAATAATTATGGGCCGTACCA
GTTTCCAGAAAAAGTCATCCCCCTATTTGTCACGAATTTAATAGAAGATAAAGAGGTACCCCTGTACGGGGATGGTACGA
ATGTCCGGGATTGGTTGCATGTTGATGACCATTGTCGTGGTATTTACATTGTGTTGACAAAGGGTAGGTCTGGTGAAATC
TACAATATCGGCGGGGGGCTTGAGTTGAGCAATGCCGATCTTAGTCAAATTATTCTCGAGGTGATGGAGAAAAGCTGGTC
GATGGTAAAGAGGGTTAAAGATCGCCCCGGTCACGACAAGAGGTATAGTGTGTCAATAAACAAGATCTCTACGCTCGGTT
ATAGGCCCGAGGTTTCATTTGAACAAGGTCTTGCTGAGACAATCAATTGGTATAAGGATAATAGCTCTTGGTGGAAGCCG
CTAAAGGCTAGGGCGGCTCTTTGA

Upstream 100 bases:

>100_bases
TGCTCAAGATGCATGCAGTGTGCGACTGTTTATCTGTTGGCGAAATAATGGCATAGACTTGAAAATTCCAAATCTAGATA
GCGGTTGAGATGGGCTTGTT

Downstream 100 bases:

>100_bases
GTTTAAAGTGCCTTATAACCGGTGGCGGTGGTATGTTGGGGCGGGATATACGGTCTGTGTTCCCAGAGAGCGTTGCCCTA
ACGCATGACGAGCTTGATAT

Product: dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTHILVTGGAGFIGSNFVRRAIENKYPEIRDCTVTVLDALTYSGNINNLSPVRSSSRLRFIHGDIRDEDLLSEIMPNVNY
VVHFAAETHVDRSIKNASAFVETNVLGTGRLLEAAARNNIDRFIHISTDEVYGSIESGSWDENQPLLPNSPYSASKAASD
LLVRSYHKTYDLPVCITRCSNNYGPYQFPEKVIPLFVTNLIEDKEVPLYGDGTNVRDWLHVDDHCRGIYIVLTKGRSGEI
YNIGGGLELSNADLSQIILEVMEKSWSMVKRVKDRPGHDKRYSVSINKISTLGYRPEVSFEQGLAETINWYKDNSSWWKP
LKARAAL

Sequences:

>Translated_327_residues
MTHILVTGGAGFIGSNFVRRAIENKYPEIRDCTVTVLDALTYSGNINNLSPVRSSSRLRFIHGDIRDEDLLSEIMPNVNY
VVHFAAETHVDRSIKNASAFVETNVLGTGRLLEAAARNNIDRFIHISTDEVYGSIESGSWDENQPLLPNSPYSASKAASD
LLVRSYHKTYDLPVCITRCSNNYGPYQFPEKVIPLFVTNLIEDKEVPLYGDGTNVRDWLHVDDHCRGIYIVLTKGRSGEI
YNIGGGLELSNADLSQIILEVMEKSWSMVKRVKDRPGHDKRYSVSINKISTLGYRPEVSFEQGLAETINWYKDNSSWWKP
LKARAAL
>Mature_326_residues
THILVTGGAGFIGSNFVRRAIENKYPEIRDCTVTVLDALTYSGNINNLSPVRSSSRLRFIHGDIRDEDLLSEIMPNVNYV
VHFAAETHVDRSIKNASAFVETNVLGTGRLLEAAARNNIDRFIHISTDEVYGSIESGSWDENQPLLPNSPYSASKAASDL
LVRSYHKTYDLPVCITRCSNNYGPYQFPEKVIPLFVTNLIEDKEVPLYGDGTNVRDWLHVDDHCRGIYIVLTKGRSGEIY
NIGGGLELSNADLSQIILEVMEKSWSMVKRVKDRPGHDKRYSVSINKISTLGYRPEVSFEQGLAETINWYKDNSSWWKPL
KARAAL

Specific function: Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4- hexulose via a three-step process involving oxidation, dehydration and re

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=324, Percent_Identity=39.8148148148148, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=24.1071428571429, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=46.9740634005764, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=46.4387464387464, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1786974, Length=278, Percent_Identity=29.4964028776978, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788366, Length=220, Percent_Identity=25.4545454545455, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788365, Length=244, Percent_Identity=24.1803278688525, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=326, Percent_Identity=35.5828220858896, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=34.8909657320872, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=317, Percent_Identity=23.9747634069401, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=346, Percent_Identity=25.4335260115607, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982035, Length=344, Percent_Identity=26.1627906976744, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=267, Percent_Identity=26.5917602996255, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=273, Percent_Identity=26.3736263736264, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=267, Percent_Identity=26.5917602996255, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=267, Percent_Identity=26.5917602996255, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=273, Percent_Identity=26.3736263736264, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=282, Percent_Identity=22.3404255319149, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=250, Percent_Identity=28.8, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=25.1572327044025, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=26.1494252873563, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24158427, Length=268, Percent_Identity=24.6268656716418, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36789; Mature: 36658

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHILVTGGAGFIGSNFVRRAIENKYPEIRDCTVTVLDALTYSGNINNLSPVRSSSRLRF
CCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IHGDIRDEDLLSEIMPNVNYVVHFAAETHVDRSIKNASAFVETNVLGTGRLLEAAARNNI
EECCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCC
DRFIHISTDEVYGSIESGSWDENQPLLPNSPYSASKAASDLLVRSYHKTYDLPVCITRCS
CEEEEEEHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
NNYGPYQFPEKVIPLFVTNLIEDKEVPLYGDGTNVRDWLHVDDHCRGIYIVLTKGRSGEI
CCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCEEEEEEEECCCCCE
YNIGGGLELSNADLSQIILEVMEKSWSMVKRVKDRPGHDKRYSVSINKISTLGYRPEVSF
EECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCH
EQGLAETINWYKDNSSWWKPLKARAAL
HHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
THILVTGGAGFIGSNFVRRAIENKYPEIRDCTVTVLDALTYSGNINNLSPVRSSSRLRF
CEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IHGDIRDEDLLSEIMPNVNYVVHFAAETHVDRSIKNASAFVETNVLGTGRLLEAAARNNI
EECCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCC
DRFIHISTDEVYGSIESGSWDENQPLLPNSPYSASKAASDLLVRSYHKTYDLPVCITRCS
CEEEEEEHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
NNYGPYQFPEKVIPLFVTNLIEDKEVPLYGDGTNVRDWLHVDDHCRGIYIVLTKGRSGEI
CCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCEEEEEEEECCCCCE
YNIGGGLELSNADLSQIILEVMEKSWSMVKRVKDRPGHDKRYSVSINKISTLGYRPEVSF
EECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCH
EQGLAETINWYKDNSSWWKPLKARAAL
HHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10196166 [H]