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The map label for this gene is strL [H]

Identifier: 28572206

GI number: 28572206

Start: 29804

End: 30667

Strand: Reverse

Name: strL [H]

Synonym: TW032

Alternate gene names: 28572206

Gene position: 30667-29804 (Counterclockwise)

Preceding gene: 28572207

Following gene: 28572205

Centisome position: 3.31

GC content: 48.84

Gene sequence:

>864_bases
TTGAGTTTAAAGTGCCTTATAACCGGTGGCGGTGGTATGTTGGGGCGGGATATACGGTCTGTGTTCCCAGAGAGCGTTGC
CCTAACGCATGACGAGCTTGATATAACGGATGAATATGCTGTTCTTGACGCCGCCAGAAATAAAGACCTTTTAATTAATT
GTGCTGCATATACACAGGTTGATGCGGCAGAAAGTAATGCTGCAAAAGCCTATGCGGTAAATGAAGCGGGTGCAAGGGCT
GTCGCCAAAGCTGCAGCAAGGCGTTCAATTCGAGTAGTGCATATCTCAACTGATTATGTTTTTTCAGGAACGGCAATACG
TCCATACCCAGAAGACCACCCGCATTCTCCCCTTTCGGTCTACGGAAAATCTAAGGCTGCTGGAGAAAAGGCAGTGTTGG
AGGAATATTCCAAAGGAAGCTTTATCATCCGTACTGCTTGGCTCTATGGACAATACGGCAATAACTTCGTAAAAAGCATT
CTGAAAGCGTGTAGAACACAGGATATCGTCACAGTTGTTAACGACCAATATGGGCAACCAACTTGGAGTCTGGACCTTGC
AAAACAGATAAAGCTTCTTGCCGAGAGTGACGCTCCATGTGGCGTGTACCATGGAACAAACAGCGGTAAAACAACCTGGT
TTGATTTTGCCGGGAAAGTGGCTGCCTTAGGTGGGTACGACCCGAAACGCATTATTCCCATTAGCTCCGCACAATTTGTG
CGCCCAGCACGCAGGCCTGGATACTCTTGTCTGGGCCATGATGCATGGACTGCAGTTGGCCTGGAACCCATGAGACCTTG
GGAAGATGCCCTGAATGATGCATGGGCTGCGGGTGTGTTTGCGGAGTGTATAGCTGGGCAGTGA

Upstream 100 bases:

>100_bases
ATAGGCCCGAGGTTTCATTTGAACAAGGTCTTGCTGAGACAATCAATTGGTATAAGGATAATAGCTCTTGGTGGAAGCCG
CTAAAGGCTAGGGCGGCTCT

Downstream 100 bases:

>100_bases
GTAGTGAATAAGTAGTAATACATACAATCACATTCTCTAACAGGTATGTTTCTTTCTTCTGTATATGTGATCTATTCCTG
TGTTATTCTTGTGCTTAAGA

Product: polysaccharide biosynthesis protein

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSLKCLITGGGGMLGRDIRSVFPESVALTHDELDITDEYAVLDAARNKDLLINCAAYTQVDAAESNAAKAYAVNEAGARA
VAKAAARRSIRVVHISTDYVFSGTAIRPYPEDHPHSPLSVYGKSKAAGEKAVLEEYSKGSFIIRTAWLYGQYGNNFVKSI
LKACRTQDIVTVVNDQYGQPTWSLDLAKQIKLLAESDAPCGVYHGTNSGKTTWFDFAGKVAALGGYDPKRIIPISSAQFV
RPARRPGYSCLGHDAWTAVGLEPMRPWEDALNDAWAAGVFAECIAGQ

Sequences:

>Translated_287_residues
MSLKCLITGGGGMLGRDIRSVFPESVALTHDELDITDEYAVLDAARNKDLLINCAAYTQVDAAESNAAKAYAVNEAGARA
VAKAAARRSIRVVHISTDYVFSGTAIRPYPEDHPHSPLSVYGKSKAAGEKAVLEEYSKGSFIIRTAWLYGQYGNNFVKSI
LKACRTQDIVTVVNDQYGQPTWSLDLAKQIKLLAESDAPCGVYHGTNSGKTTWFDFAGKVAALGGYDPKRIIPISSAQFV
RPARRPGYSCLGHDAWTAVGLEPMRPWEDALNDAWAAGVFAECIAGQ
>Mature_286_residues
SLKCLITGGGGMLGRDIRSVFPESVALTHDELDITDEYAVLDAARNKDLLINCAAYTQVDAAESNAAKAYAVNEAGARAV
AKAAARRSIRVVHISTDYVFSGTAIRPYPEDHPHSPLSVYGKSKAAGEKAVLEEYSKGSFIIRTAWLYGQYGNNFVKSIL
KACRTQDIVTVVNDQYGQPTWSLDLAKQIKLLAESDAPCGVYHGTNSGKTTWFDFAGKVAALGGYDPKRIIPISSAQFVR
PARRPGYSCLGHDAWTAVGLEPMRPWEDALNDAWAAGVFAECIAGQ

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=312, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI11034825, Length=312, Percent_Identity=26.6025641025641, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1788352, Length=272, Percent_Identity=34.1911764705882, Blast_Score=140, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31009; Mature: 30878

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKCLITGGGGMLGRDIRSVFPESVALTHDELDITDEYAVLDAARNKDLLINCAAYTQV
CCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCHHHEEHHCCCCCEEEEEEHHCEE
DAAESNAAKAYAVNEAGARAVAKAAARRSIRVVHISTDYVFSGTAIRPYPEDHPHSPLSV
CCCCCCCCEEEEECHHHHHHHHHHHHHCCEEEEEEECCEEEECCEECCCCCCCCCCCCEE
YGKSKAAGEKAVLEEYSKGSFIIRTAWLYGQYGNNFVKSILKACRTQDIVTVVNDQYGQP
CCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
TWSLDLAKQIKLLAESDAPCGVYHGTNSGKTTWFDFAGKVAALGGYDPKRIIPISSAQFV
EEEHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCEEEECCCCCCEEEECCCHHHH
RPARRPGYSCLGHDAWTAVGLEPMRPWEDALNDAWAAGVFAECIAGQ
CCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLKCLITGGGGMLGRDIRSVFPESVALTHDELDITDEYAVLDAARNKDLLINCAAYTQV
CEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCHHHEEHHCCCCCEEEEEEHHCEE
DAAESNAAKAYAVNEAGARAVAKAAARRSIRVVHISTDYVFSGTAIRPYPEDHPHSPLSV
CCCCCCCCEEEEECHHHHHHHHHHHHHCCEEEEEEECCEEEECCEECCCCCCCCCCCCEE
YGKSKAAGEKAVLEEYSKGSFIIRTAWLYGQYGNNFVKSILKACRTQDIVTVVNDQYGQP
CCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
TWSLDLAKQIKLLAESDAPCGVYHGTNSGKTTWFDFAGKVAALGGYDPKRIIPISSAQFV
EEEHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCEEEECCCCCCEEEECCCHHHH
RPARRPGYSCLGHDAWTAVGLEPMRPWEDALNDAWAAGVFAECIAGQ
CCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661369 [H]