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The map label for this gene is gyrB [H]

Identifier: 28572180

GI number: 28572180

Start: 4485

End: 6434

Strand: Direct

Name: gyrB [H]

Synonym: TW005

Alternate gene names: 28572180

Gene position: 4485-6434 (Clockwise)

Preceding gene: 28572179

Following gene: 28572181

Centisome position: 0.48

GC content: 47.59

Gene sequence:

>1950_bases
ATGGTTGACACACCCGAGCCCGCATATGGCGCTGATCAGATACAGGTTCTCGAGGGTTTAGAAGCCGTTCGGCGAAGACC
GGGTATGTACATAGGCAGTACCGGGTCCCGGGGTTTACACCATTTGGTATATGAGCTTGTTGATAACTCAGTAGACGAAG
CCCTTGCTGGCTATTGCACAAAAATAAATGTTGCTATTCTCGCAGACGGCGGGGTTAGGGTGACGGATAACGGCAGGGGC
ATCCCTGTTGATATTCATCCTAAAGAGGGGGTTTCCACAGTTCAGGTTGTTCTCACAACCCTTCACGCTGGCGGAAAATT
CGGTAGTGGAGGATACTCCGTTTCCGGCGGTTTGCATGGCGTTGGAAGCTCTGTTGTGAATGCGTTGTCTAAGAGACTGT
ATGTTGAGGTCTCAAGACAGGGTTTTGTTTTTAGACAAAATTATGAGGGCGGTAACCCCACTGCTGCACTTGAAAGATGT
GAAGAAACCAATGCTACCGGAACGGCAATTACCTTTTGGCCTGATGATGATATTTTTGAAACCGTTTGTTTTGAATATGA
AGTGCTGCGCTCTCGCCTGCAACAGATGGCCTTCCTGAATAGCGGCCTTACTGTCTCCATAACAGACGAGAGGGTGGACA
GCCCGACTAACAACGAGACTTACTGTTATCAAAACGGTCTGTTTGACTATGTTATGTATCAAAACCGCATAAGAAAACAT
GAACCTCTTCATGAAGAAATAATTCACTTTCAGGCAGAGAACAAGGATCGCCTGATGTCCCTTGAGGTTGCGATGCGGTG
GACCTCGTCTTCGACCGAGAATATATATACATTTGCGAACACAATTAACACCCATGAGGGCGGTACACACGAAGAGGGCT
TCAGGGCAGCACTCACATCGCTCGTAAACCGTTATGCACGCGAGAAAGGTTTTCTAAAGGAAAAGGACGAAAGCCTTCAG
GGTGAGGATATTAGGGAAGGGCTTACAGCAATTATCTCTGTTAAGCTTGGCGAACCACAGTTTGAGGGTCAAACAAAAAC
AAAGCTTGGCAATACAGAAATGCGTGGCTATGTTCACCACGAGGTGAACTCTTATCTGTGGGATTGGTTTGACAGTCACC
CCGCTCAGGCAAAGGTGATTATTTCAAAAGCCCTTCTTGCGGCTGAGGCTAGATTGGCAGCTAAGCGCGCCAGAGAGGCC
TCGAGAAAACGGGGTTTATTCGAGTCTGTTTCAATGCCAGGCAAGCTGAAGGAGTGTCAGGCAAAGGACCCGGCCGTTTC
AGAACTTTTTATTGTTGAGGGGGATTCCGCAGGTGGGTCTGCAATACAGGGCAGGGATCCGCTCACCCAGGCCGTTTTAC
CGCTCAGGGGAAAAATACTTAATGTTGAAAAGGCAAGGCTCGACAAGGCTATTGCAAATTCTGAAATACAGGCTATGCTC
ACGGCTTTCGGTTCTGGTATAGCTGAGGATTTTGATGAATCAAAGGCTCGCTACCACAAAATAATTCTTATGTCCGATGC
AGATGTCGATGGCCAGCATATAACCACACTTCTGCTCACACTCCTGTTCAGGTATATGAGGCCACTTATAGAGAGTGGTT
ATGTGTATTTGGCTCACCCCCCCTTGTACAGGATTAAGTGGAGTAATTCCGAGCACGACTATGTTTTTAGCGACAGAGAA
AGAGATAAGAAGCTCGCAGAAGGTAAGCTCTCCGGAAAAAAGATACCAAAAGAAAACGCAATTCAGCGATACAAGGGCCT
TGGAGAAATGAATTATAGAGAGCTGTGGGACACCACCATGAATCCGGCAACTCGCACCCTCCGCCAGATTACCGTTGACG
ACGTTACGTTAGCTGATGAGATTTTTTCTGTGCTTATGGGGGAGGATGTCGACTCTCGTCGTAAGTTCATCCAGCACAAC
GCCAGAGATGTTCGCTTTTTGGATATATAG

Upstream 100 bases:

>100_bases
ATCAAGGAGGGGTTATTTTGGCCCGAAGCTTGTCAGAAACGTGGATAAACACTACGAATGATAGAATCTTGCGTTAAGGT
CCTTTAGGAGTTCTGCTTGC

Downstream 100 bases:

>100_bases
ATATTGTCTGAGCAAAATATTAACCAAGTAGATCTTCGCGAGGAGATGCAGCGGTCGTATCTCGACTACGCCATGAGCGT
TATAATCGGTCGTGCCTTAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MVDTPEPAYGADQIQVLEGLEAVRRRPGMYIGSTGSRGLHHLVYELVDNSVDEALAGYCTKINVAILADGGVRVTDNGRG
IPVDIHPKEGVSTVQVVLTTLHAGGKFGSGGYSVSGGLHGVGSSVVNALSKRLYVEVSRQGFVFRQNYEGGNPTAALERC
EETNATGTAITFWPDDDIFETVCFEYEVLRSRLQQMAFLNSGLTVSITDERVDSPTNNETYCYQNGLFDYVMYQNRIRKH
EPLHEEIIHFQAENKDRLMSLEVAMRWTSSSTENIYTFANTINTHEGGTHEEGFRAALTSLVNRYAREKGFLKEKDESLQ
GEDIREGLTAIISVKLGEPQFEGQTKTKLGNTEMRGYVHHEVNSYLWDWFDSHPAQAKVIISKALLAAEARLAAKRAREA
SRKRGLFESVSMPGKLKECQAKDPAVSELFIVEGDSAGGSAIQGRDPLTQAVLPLRGKILNVEKARLDKAIANSEIQAML
TAFGSGIAEDFDESKARYHKIILMSDADVDGQHITTLLLTLLFRYMRPLIESGYVYLAHPPLYRIKWSNSEHDYVFSDRE
RDKKLAEGKLSGKKIPKENAIQRYKGLGEMNYRELWDTTMNPATRTLRQITVDDVTLADEIFSVLMGEDVDSRRKFIQHN
ARDVRFLDI

Sequences:

>Translated_649_residues
MVDTPEPAYGADQIQVLEGLEAVRRRPGMYIGSTGSRGLHHLVYELVDNSVDEALAGYCTKINVAILADGGVRVTDNGRG
IPVDIHPKEGVSTVQVVLTTLHAGGKFGSGGYSVSGGLHGVGSSVVNALSKRLYVEVSRQGFVFRQNYEGGNPTAALERC
EETNATGTAITFWPDDDIFETVCFEYEVLRSRLQQMAFLNSGLTVSITDERVDSPTNNETYCYQNGLFDYVMYQNRIRKH
EPLHEEIIHFQAENKDRLMSLEVAMRWTSSSTENIYTFANTINTHEGGTHEEGFRAALTSLVNRYAREKGFLKEKDESLQ
GEDIREGLTAIISVKLGEPQFEGQTKTKLGNTEMRGYVHHEVNSYLWDWFDSHPAQAKVIISKALLAAEARLAAKRAREA
SRKRGLFESVSMPGKLKECQAKDPAVSELFIVEGDSAGGSAIQGRDPLTQAVLPLRGKILNVEKARLDKAIANSEIQAML
TAFGSGIAEDFDESKARYHKIILMSDADVDGQHITTLLLTLLFRYMRPLIESGYVYLAHPPLYRIKWSNSEHDYVFSDRE
RDKKLAEGKLSGKKIPKENAIQRYKGLGEMNYRELWDTTMNPATRTLRQITVDDVTLADEIFSVLMGEDVDSRRKFIQHN
ARDVRFLDI
>Mature_649_residues
MVDTPEPAYGADQIQVLEGLEAVRRRPGMYIGSTGSRGLHHLVYELVDNSVDEALAGYCTKINVAILADGGVRVTDNGRG
IPVDIHPKEGVSTVQVVLTTLHAGGKFGSGGYSVSGGLHGVGSSVVNALSKRLYVEVSRQGFVFRQNYEGGNPTAALERC
EETNATGTAITFWPDDDIFETVCFEYEVLRSRLQQMAFLNSGLTVSITDERVDSPTNNETYCYQNGLFDYVMYQNRIRKH
EPLHEEIIHFQAENKDRLMSLEVAMRWTSSSTENIYTFANTINTHEGGTHEEGFRAALTSLVNRYAREKGFLKEKDESLQ
GEDIREGLTAIISVKLGEPQFEGQTKTKLGNTEMRGYVHHEVNSYLWDWFDSHPAQAKVIISKALLAAEARLAAKRAREA
SRKRGLFESVSMPGKLKECQAKDPAVSELFIVEGDSAGGSAIQGRDPLTQAVLPLRGKILNVEKARLDKAIANSEIQAML
TAFGSGIAEDFDESKARYHKIILMSDADVDGQHITTLLLTLLFRYMRPLIESGYVYLAHPPLYRIKWSNSEHDYVFSDRE
RDKKLAEGKLSGKKIPKENAIQRYKGLGEMNYRELWDTTMNPATRTLRQITVDDVTLADEIFSVLMGEDVDSRRKFIQHN
ARDVRFLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=617, Percent_Identity=23.0145867098865, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI19913406, Length=630, Percent_Identity=21.4285714285714, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=49.0161001788909, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1789408, Length=639, Percent_Identity=39.5931142410016, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI212645845, Length=550, Percent_Identity=24.3636363636364, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17535065, Length=573, Percent_Identity=23.3856893542757, Blast_Score=106, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324241, Length=647, Percent_Identity=23.9567233384853, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17136538, Length=469, Percent_Identity=23.6673773987207, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72581; Mature: 72581

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTPEPAYGADQIQVLEGLEAVRRRPGMYIGSTGSRGLHHLVYELVDNSVDEALAGYCT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHH
KINVAILADGGVRVTDNGRGIPVDIHPKEGVSTVQVVLTTLHAGGKFGSGGYSVSGGLHG
EEEEEEEECCCEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCH
VGSSVVNALSKRLYVEVSRQGFVFRQNYEGGNPTAALERCEETNATGTAITFWPDDDIFE
HHHHHHHHHHHHEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHH
TVCFEYEVLRSRLQQMAFLNSGLTVSITDERVDSPTNNETYCYQNGLFDYVMYQNRIRKH
HHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHC
EPLHEEIIHFQAENKDRLMSLEVAMRWTSSSTENIYTFANTINTHEGGTHEEGFRAALTS
CCHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
LVNRYAREKGFLKEKDESLQGEDIREGLTAIISVKLGEPQFEGQTKTKLGNTEMRGYVHH
HHHHHHHHCCCHHCCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCHHCCCCCHHHHHHHH
EVNSYLWDWFDSHPAQAKVIISKALLAAEARLAAKRAREASRKRGLFESVSMPGKLKECQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHCC
AKDPAVSELFIVEGDSAGGSAIQGRDPLTQAVLPLRGKILNVEKARLDKAIANSEIQAML
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHH
TAFGSGIAEDFDESKARYHKIILMSDADVDGQHITTLLLTLLFRYMRPLIESGYVYLAHP
HHHCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYRIKWSNSEHDYVFSDRERDKKLAEGKLSGKKIPKENAIQRYKGLGEMNYRELWDTTM
CEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCC
NPATRTLRQITVDDVTLADEIFSVLMGEDVDSRRKFIQHNARDVRFLDI
CHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MVDTPEPAYGADQIQVLEGLEAVRRRPGMYIGSTGSRGLHHLVYELVDNSVDEALAGYCT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHH
KINVAILADGGVRVTDNGRGIPVDIHPKEGVSTVQVVLTTLHAGGKFGSGGYSVSGGLHG
EEEEEEEECCCEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCH
VGSSVVNALSKRLYVEVSRQGFVFRQNYEGGNPTAALERCEETNATGTAITFWPDDDIFE
HHHHHHHHHHHHEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHH
TVCFEYEVLRSRLQQMAFLNSGLTVSITDERVDSPTNNETYCYQNGLFDYVMYQNRIRKH
HHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHC
EPLHEEIIHFQAENKDRLMSLEVAMRWTSSSTENIYTFANTINTHEGGTHEEGFRAALTS
CCHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
LVNRYAREKGFLKEKDESLQGEDIREGLTAIISVKLGEPQFEGQTKTKLGNTEMRGYVHH
HHHHHHHHCCCHHCCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCHHCCCCCHHHHHHHH
EVNSYLWDWFDSHPAQAKVIISKALLAAEARLAAKRAREASRKRGLFESVSMPGKLKECQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHCC
AKDPAVSELFIVEGDSAGGSAIQGRDPLTQAVLPLRGKILNVEKARLDKAIANSEIQAML
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHH
TAFGSGIAEDFDESKARYHKIILMSDADVDGQHITTLLLTLLFRYMRPLIESGYVYLAHP
HHHCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYRIKWSNSEHDYVFSDRERDKKLAEGKLSGKKIPKENAIQRYKGLGEMNYRELWDTTM
CEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCC
NPATRTLRQITVDDVTLADEIFSVLMGEDVDSRRKFIQHNARDVRFLDI
CHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8392138 [H]