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The map label for this gene is thiB [H]

Identifier: 326423753

GI number: 326423753

Start: 720684

End: 721712

Strand: Reverse

Name: thiB [H]

Synonym: NA

Alternate gene names: 326423753

Gene position: 721712-720684 (Counterclockwise)

Preceding gene: 27364160

Following gene: 326423752

Centisome position: 21.99

GC content: 49.85

Gene sequence:

>1029_bases
ATGCCAAATGGAGAGCGAGCAGTGAAACACACTCTAAACCTGATAGCCCTAGCGTCAATCACGTCAATGATGGGCATCTC
TTCTTCTGCACTTGCGGCGGACAAAACCTTAACCATTTATACCTACGATTCTTTTGCTTCCGATTGGGGCCCTGGCCCGA
CAGTCGAAAAAGCATTTGAAGCTCAGTGCGGCTGCGATGTGAATTTTGTCGCGCTGGACGATGGGGTGTCAATTCTCAAC
CGCTTACGTTTAGAAGGCAGCAATACCAAAGCGGATATTGTTTTAGGTTTGGATAACAATTTGATGGCAGAAGCTAAAGC
CACAGGTTTGCTGGCGCCACATCAAGTGAACACCCAAGCGCTGTCATTGCCAAATGGTTGGGCTGACGACACGTTTATTC
CTTATGACTACGGTTACTTTGCGTTTGTTTACAACAAAGGCAAACTGGCGAATCCACCCAAGAGCTTAAAAGAGCTGGTG
GAATCACGCGACGATCTGAAAGTCATCTACCAAGACCCTCGCACTTCAACACCCGGTCAAGGTTTGATGCTGTGGATAAA
GTCAGTGTATGGCGATGACGTGGCTGGAGCATGGCAGCAACTGGCGAAGAAAACCGTGACGGTCACGAAAGGTTGGTCGG
AAGCGTACTCGATGTTCCTCAAAGGCGAATCGGACATGGTGCTCTCTTACACCACCTCACCTGCGTACCACCTGATTGCC
GAAAATGATGCGCAATATGCCGCGGCGGGTTTTAGTGAAGGACACTACATGCAAGTGGAAGTCGCCGCAAAAGTGAAAAG
TAGCCAGAACTCAGCGCTGGCTGATCAGTTTATGAGTTTCATTTTGAGTGATGAGTTCCAAAGCGCAATGCCTACGGGCA
ACTGGATGTACCCAGTGACCAACGTGACGCTGCCAGAGGGCTACCGCAGTTTAACCGTGCCTGAGAAGGCGTTGAGTTTC
AGTGCGGAAGAGGTAGCGAAGCAGCGTAAAGCTTGGATTCGCGAATGGCAAAGCGCGTTGACGTTTTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: thiamin/thiamin pyrophosphate ABC transporter thiamin/thiamin pyrophospate-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:
NA

Sequences:

>Translated_342_residues
MPNGERAVKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDVNFVALDDGVSILN
RLRLEGSNTKADIVLGLDNNLMAEAKATGLLAPHQVNTQALSLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELV
ESRDDLKVIYQDPRTSTPGQGLMLWIKSVYGDDVAGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIA
ENDAQYAAAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEGYRSLTVPEKALSF
SAEEVAKQRKAWIREWQSALTF
>Mature_341_residues
PNGERAVKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDVNFVALDDGVSILNR
LRLEGSNTKADIVLGLDNNLMAEAKATGLLAPHQVNTQALSLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVE
SRDDLKVIYQDPRTSTPGQGLMLWIKSVYGDDVAGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIAE
NDAQYAAAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEGYRSLTVPEKALSFS
AEEVAKQRKAWIREWQSALTF

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=309, Percent_Identity=65.6957928802589, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37492; Mature: 37360

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGERAVKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCHHCCCCCCCCHHHHHH
AQCGCDVNFVALDDGVSILNRLRLEGSNTKADIVLGLDNNLMAEAKATGLLAPHQVNTQA
HHCCCCEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCEE
LSLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLKVIYQDPRTSTPGQ
EECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
GLMLWIKSVYGDDVAGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIA
HHHHHHHHHHCCCHHHHHHHHHHHEEEEECCHHHHHHHHHCCCCCEEEEEECCCEEEEEE
ENDAQYAAAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVT
CCCCCEEECCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
NVTLPEGYRSLTVPEKALSFSAEEVAKQRKAWIREWQSALTF
EEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNGERAVKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCHHCCCCCCCCHHHHHH
AQCGCDVNFVALDDGVSILNRLRLEGSNTKADIVLGLDNNLMAEAKATGLLAPHQVNTQA
HHCCCCEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCEE
LSLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLKVIYQDPRTSTPGQ
EECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
GLMLWIKSVYGDDVAGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIA
HHHHHHHHHHCCCHHHHHHHHHHHEEEEECCHHHHHHHHHCCCCCEEEEEECCCEEEEEE
ENDAQYAAAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVT
CCCCCEEECCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
NVTLPEGYRSLTVPEKALSFSAEEVAKQRKAWIREWQSALTF
EEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]