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The map label for this gene is ptsP [H]

Identifier: 326423726

GI number: 326423726

Start: 517766

End: 520012

Strand: Reverse

Name: ptsP [H]

Synonym: NA

Alternate gene names: 326423726

Gene position: 520012-517766 (Counterclockwise)

Preceding gene: 27363983

Following gene: 27363981

Centisome position: 15.85

GC content: 48.38

Gene sequence:

>2247_bases
ATGCTGAGTCAACTCAGGGAGATAGTTGAAAAGGTTTCTCGTGTCGAGGATGTTCACCAAGCATTGAATACGTTGGTACG
AGAAACTTGCACAGCATTGAGCACAGAGTGTTGCACGATTTATCTGGCGAATGAAGAAAGGCAACGACTAGAGCTAATGG
CAACGCAAGGCCTGACTTTTCATGGCGACAGTATTCACATCCAGTTTGAAGAGGGCTTGGTTGGCTTAGTGAAACGCAGC
GCCGAGCCTCTTAACTTAGCCGAAGCCTCGAAGCATCCCGCTTTTAAATATTTTCCTCAGTTAGGCGAAGAAACGTATCA
CTCGTTTTTAGGTGCGCCGATCATCCATCGTAAAAAAGTATTGGGCGTGCTGGTTATCCAGCAGAAAACCCCACGACTTT
TCAGCGAGATGGAAGAATCCTTCCTTGTCACTCTATCCGCTCAGTTAGCCGTCATCATTGCGCATGCACAAAGTCTTGGC
CATTGGTCATTGGCGTCTAAACCTGCTGCGGTGCAGGGCATTGCCGCTTCAAGTGGGGTCGCAATTGGCGAGTTTTGGTA
TGACAATTCACAGCCGATATTGTCGGAGGTGTGCCCAGCCTCGACACTCGATAAAGAGCGAGAGCAAGAGTGGTTAGCTG
TTGCGATTGAAGCCGCGTTGGCGGATTTTCGCCGTATGCGCAAAAAGTTCGACAGTGAAATCAATAAAGATGCGCTGGCC
ATATTCGATCTCTTCACGCACTTGCTGAACGATCCTATGTTACGTACAGACTTGAAAAAGCATGTGGAGCGCGGAGATCG
CGCGGACTGGGCATTGCGTCAAGTGGTCGAAAGCTATTCCAGTCGCTTTGCACGAATGTCGGATGTTTACCTGAGAGAGC
GAGCGCATGATATTCGTGAGTTAGGTCAGCGTTTACTGTTCTTTTTACACAACAGTGAGCAAGAGCAGCCAAGAATTGAG
CGACCGGTAATTTTGGTTGCCAATGAACTCACCGCTTCACTTTTGGCGTCTGTACCGAAAAAATACCTGTTGGCAGTGGT
GGCATTAGAAGGCGCGGCGAACTCCCACGCTGCGATTTTATCCAGAGCGCTAGGAGTGCCTGCTGTCATGGGGGCGAACC
TGAATCTGGAAGAGGTGCATGGCAAGCAGGGGATCGTCGATGGCTACTCCGGCGAGATTTATATTCAGCCTAATCGCCAG
ATTTTGCGTGAGTACCGCAGCCTTGTAACGGAAGAGAGTGAATTGTTCGCGATGGTGAACAAGGAGCTTGCTCTGCCCGC
GAAAACTCAGGATAACGTCGACGTCGAGGTATTACTCAACGCGGGCTTGAGCGCCGACAGTAATATCGCGATCAACGCGG
GTGTGGATGGTGTGGGATTATATCGCACTGAAATTGCTTTTCTCTTGCAACATCGTTTTCCTTCTGAGGATGAGCAATAT
CAGCAATATCGCGCTATTTTAAGTAGTTATCCTCAGCAGCGAGTGGTGATGCGCACCTTAGATATTGGCGGTGATAAACC
GCTGCCTTACCTGCCTATTGAAGAGGATAACCCCTTTTTAGGCTGGCGTGGGATCCGTTTTACCCTAGATCATCCAGACA
TCTTCCTTATCCAGTTACGAGCCATGTTACGAGCCAGTGCTGAGCATGGCAATTTGAGTATTCTCTTGCCGATGATTTCT
GGTGTCGGAGAGCTTGATGACGCCATTCGTTTGATTCAGCAAGCTTATCATGAGGTCGTGTTGCTGGATGAACGCGTGGT
GATGCCACAAATTGGCGTGATGATTGAAGTCCCTTCGATGGTCTATTTGATTCCATCAATTGCTCAGCGCGTGGATTTTG
TCTCGGTGGGGACAAACGACTTAACGCAATATTTATTGGCTGTGGATCGGAACAATGCACGAGTTTCTGATGTCTATGAA
TCGTTGCATCCAGCGGTATTGCAAGCCCTGCAGCAGATTTATCAGCAGTGTGGACAATACCACTTACCCGTTTGTATTTG
TGGCGAGTTAGCGGGAGACCCATTTGGCGCATTACTGCTACTTGGTTTGGGTTACCGTAGTTTGAGCATGAACACCTCAA
ACGTCGCGCGGATCAAATATTTGATTCGCCATAGCCAATTGAGCGATTTACAAGCCTTGTCTCAACAAGCTTTGTCGCTT
TCTTATGGCAAGGATATTCACCAGTTGATGCGGGATTATTTTATTAATCATGATTTTGCCGGTTTTATCCGTGCAGGTAA
GAAGTAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: phosphocarrier protein kinase/phosphorylased

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:
NA

Sequences:

>Translated_748_residues
MLSQLREIVEKVSRVEDVHQALNTLVRETCTALSTECCTIYLANEERQRLELMATQGLTFHGDSIHIQFEEGLVGLVKRS
AEPLNLAEASKHPAFKYFPQLGEETYHSFLGAPIIHRKKVLGVLVIQQKTPRLFSEMEESFLVTLSAQLAVIIAHAQSLG
HWSLASKPAAVQGIAASSGVAIGEFWYDNSQPILSEVCPASTLDKEREQEWLAVAIEAALADFRRMRKKFDSEINKDALA
IFDLFTHLLNDPMLRTDLKKHVERGDRADWALRQVVESYSSRFARMSDVYLRERAHDIRELGQRLLFFLHNSEQEQPRIE
RPVILVANELTASLLASVPKKYLLAVVALEGAANSHAAILSRALGVPAVMGANLNLEEVHGKQGIVDGYSGEIYIQPNRQ
ILREYRSLVTEESELFAMVNKELALPAKTQDNVDVEVLLNAGLSADSNIAINAGVDGVGLYRTEIAFLLQHRFPSEDEQY
QQYRAILSSYPQQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLIQLRAMLRASAEHGNLSILLPMIS
GVGELDDAIRLIQQAYHEVVLLDERVVMPQIGVMIEVPSMVYLIPSIAQRVDFVSVGTNDLTQYLLAVDRNNARVSDVYE
SLHPAVLQALQQIYQQCGQYHLPVCICGELAGDPFGALLLLGLGYRSLSMNTSNVARIKYLIRHSQLSDLQALSQQALSL
SYGKDIHQLMRDYFINHDFAGFIRAGKK
>Mature_748_residues
MLSQLREIVEKVSRVEDVHQALNTLVRETCTALSTECCTIYLANEERQRLELMATQGLTFHGDSIHIQFEEGLVGLVKRS
AEPLNLAEASKHPAFKYFPQLGEETYHSFLGAPIIHRKKVLGVLVIQQKTPRLFSEMEESFLVTLSAQLAVIIAHAQSLG
HWSLASKPAAVQGIAASSGVAIGEFWYDNSQPILSEVCPASTLDKEREQEWLAVAIEAALADFRRMRKKFDSEINKDALA
IFDLFTHLLNDPMLRTDLKKHVERGDRADWALRQVVESYSSRFARMSDVYLRERAHDIRELGQRLLFFLHNSEQEQPRIE
RPVILVANELTASLLASVPKKYLLAVVALEGAANSHAAILSRALGVPAVMGANLNLEEVHGKQGIVDGYSGEIYIQPNRQ
ILREYRSLVTEESELFAMVNKELALPAKTQDNVDVEVLLNAGLSADSNIAINAGVDGVGLYRTEIAFLLQHRFPSEDEQY
QQYRAILSSYPQQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLIQLRAMLRASAEHGNLSILLPMIS
GVGELDDAIRLIQQAYHEVVLLDERVVMPQIGVMIEVPSMVYLIPSIAQRVDFVSVGTNDLTQYLLAVDRNNARVSDVYE
SLHPAVLQALQQIYQQCGQYHLPVCICGELAGDPFGALLLLGLGYRSLSMNTSNVARIKYLIRHSQLSDLQALSQQALSL
SYGKDIHQLMRDYFINHDFAGFIRAGKK

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=748, Percent_Identity=48.1283422459893, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=578, Percent_Identity=34.6020761245675, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI48994992, Length=515, Percent_Identity=33.3980582524272, Blast_Score=268, Evalue=1e-72,
Organism=Escherichia coli, GI1788726, Length=582, Percent_Identity=31.9587628865979, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1787994, Length=439, Percent_Identity=25.2847380410023, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI226510935, Length=202, Percent_Identity=30.1980198019802, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83812; Mature: 83812

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQLREIVEKVSRVEDVHQALNTLVRETCTALSTECCTIYLANEERQRLELMATQGLTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE
HGDSIHIQFEEGLVGLVKRSAEPLNLAEASKHPAFKYFPQLGEETYHSFLGAPIIHRKKV
ECCEEEEEECCCHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LGVLVIQQKTPRLFSEMEESFLVTLSAQLAVIIAHAQSLGHWSLASKPAAVQGIAASSGV
HEEEEECCCCHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCC
AIGEFWYDNSQPILSEVCPASTLDKEREQEWLAVAIEAALADFRRMRKKFDSEINKDALA
EEEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IFDLFTHLLNDPMLRTDLKKHVERGDRADWALRQVVESYSSRFARMSDVYLRERAHDIRE
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFFLHNSEQEQPRIERPVILVANELTASLLASVPKKYLLAVVALEGAANSHAAIL
HHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SRALGVPAVMGANLNLEEVHGKQGIVDGYSGEIYIQPNRQILREYRSLVTEESELFAMVN
HHHHCCCEEECCCCCHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHH
KELALPAKTQDNVDVEVLLNAGLSADSNIAINAGVDGVGLYRTEIAFLLQHRFPSEDEQY
HHHCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHH
QQYRAILSSYPQQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLIQLR
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCHHHHHH
AMLRASAEHGNLSILLPMISGVGELDDAIRLIQQAYHEVVLLDERVVMPQIGVMIEVPSM
HHHHHCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHEEEECCEECCCCCCEEEECCHH
VYLIPSIAQRVDFVSVGTNDLTQYLLAVDRNNARVSDVYESLHPAVLQALQQIYQQCGQY
HHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLPVCICGELAGDPFGALLLLGLGYRSLSMNTSNVARIKYLIRHSQLSDLQALSQQALSL
CCCEEEECCCCCCHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYGKDIHQLMRDYFINHDFAGFIRAGKK
HCCHHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MLSQLREIVEKVSRVEDVHQALNTLVRETCTALSTECCTIYLANEERQRLELMATQGLTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE
HGDSIHIQFEEGLVGLVKRSAEPLNLAEASKHPAFKYFPQLGEETYHSFLGAPIIHRKKV
ECCEEEEEECCCHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LGVLVIQQKTPRLFSEMEESFLVTLSAQLAVIIAHAQSLGHWSLASKPAAVQGIAASSGV
HEEEEECCCCHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCC
AIGEFWYDNSQPILSEVCPASTLDKEREQEWLAVAIEAALADFRRMRKKFDSEINKDALA
EEEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IFDLFTHLLNDPMLRTDLKKHVERGDRADWALRQVVESYSSRFARMSDVYLRERAHDIRE
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFFLHNSEQEQPRIERPVILVANELTASLLASVPKKYLLAVVALEGAANSHAAIL
HHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SRALGVPAVMGANLNLEEVHGKQGIVDGYSGEIYIQPNRQILREYRSLVTEESELFAMVN
HHHHCCCEEECCCCCHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHH
KELALPAKTQDNVDVEVLLNAGLSADSNIAINAGVDGVGLYRTEIAFLLQHRFPSEDEQY
HHHCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHH
QQYRAILSSYPQQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLIQLR
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCHHHHHH
AMLRASAEHGNLSILLPMISGVGELDDAIRLIQQAYHEVVLLDERVVMPQIGVMIEVPSM
HHHHHCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHEEEECCEECCCCCCEEEECCHH
VYLIPSIAQRVDFVSVGTNDLTQYLLAVDRNNARVSDVYESLHPAVLQALQQIYQQCGQY
HHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLPVCICGELAGDPFGALLLLGLGYRSLSMNTSNVARIKYLIRHSQLSDLQALSQQALSL
CCCEEEECCCCCCHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYGKDIHQLMRDYFINHDFAGFIRAGKK
HCCHHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]