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The map label for this gene is pleD [H]

Identifier: 27366393

GI number: 27366393

Start: 3196417

End: 3197331

Strand: Reverse

Name: pleD [H]

Synonym: VV1_3127

Alternate gene names: 27366393

Gene position: 3197331-3196417 (Counterclockwise)

Preceding gene: 27366394

Following gene: 27366386

Centisome position: 97.42

GC content: 46.56

Gene sequence:

>915_bases
ATGAAAGTAGAACCATCGAATACGGAAATTTTGGTTGTTGATGATACCGTTGATAACGTCAAGCTTTTAAGTCAGTTGCT
GCAAAACGAAGGTTATTTGGTGAAAGGCGCTTTGAGTGCCAAGAGTGCATTACGCATGTGCCAAAAAAAGAAACCTGATT
TGATATTGCTCGACATCATGATGCCAGAGGTGGATGGCTATGAACTGTGTCGACAGCTAAAAAAGGATCCCTCGACACAA
GATATCCCGGTGATCTTTCTCAGCGCCTTAGATCATGTTCAAGATAAAGTGCGCGCATTTCAGATCGGTGGTGTCGATTA
TATTCAAAAGCCTTTCGAGGTGATGGAAGTGTTGGCCAGAGTTCGCACTCATGCGGGTTTGGTGACATTGCAGCGCGCAT
TGATCGAACAAAATGAACAACTAAAAAGAATGGCGACCACCGATGCACTGACGGGATTGGTCAACCGCCGCTATCTTGCC
GATATGGCGGGGAAAGTGTCAGGGAACTTTGCTCTGATTTTGTTTGATATCGATGATTTCAAGACTATCAATGACCAATA
TGGGCATCATGTCGGAGACGAAGTTTTAGTGAGAATCGCTGAGATTACTCGCAGAGTCATTGGGCAGCATGGTTACTGCG
CAAGATGGGGTGGGGAGGAGTTCCTGATCTTACTGCCGAACTTTGATGCCAAACGTGCCTACGATCTGGCCGCTTGTATT
CAAGATGAGTTCAATGAATCGAAGGGTACGCTGGGTGCTTACCACTTCACCGCAAGCTTCGGAGTCGCATGCAATCAAGG
TGCACTCTCGTTTCATCACGTCATTCGCCAAGCTGATGCCGCAATGTATCAAGGCAAAAAGAGTGGCAAAAACCGTGTTG
TATCAAGCAGCGCCATGGAGAGCCGCGAAATTTAA

Upstream 100 bases:

>100_bases
GTCTAAACAGCTTGGTAGAGAACTATCAATTTGATGAGCTTTACGAGTTATTTCAAGAATAAAAAAGGGCATTGCGGGAA
AAGCGTCGCAATGAAAGCAG

Downstream 100 bases:

>100_bases
AGATGCGGCATTGCTAGTTAGGGCTAAAGTTGCTCTTCAATGGGTAAAATGCCAGCAAACCAAGCGCCGATTTTCCGCCA
TACCGTGGCTTCCGGTTCGG

Product: Two component response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKVEPSNTEILVVDDTVDNVKLLSQLLQNEGYLVKGALSAKSALRMCQKKKPDLILLDIMMPEVDGYELCRQLKKDPSTQ
DIPVIFLSALDHVQDKVRAFQIGGVDYIQKPFEVMEVLARVRTHAGLVTLQRALIEQNEQLKRMATTDALTGLVNRRYLA
DMAGKVSGNFALILFDIDDFKTINDQYGHHVGDEVLVRIAEITRRVIGQHGYCARWGGEEFLILLPNFDAKRAYDLAACI
QDEFNESKGTLGAYHFTASFGVACNQGALSFHHVIRQADAAMYQGKKSGKNRVVSSSAMESREI

Sequences:

>Translated_304_residues
MKVEPSNTEILVVDDTVDNVKLLSQLLQNEGYLVKGALSAKSALRMCQKKKPDLILLDIMMPEVDGYELCRQLKKDPSTQ
DIPVIFLSALDHVQDKVRAFQIGGVDYIQKPFEVMEVLARVRTHAGLVTLQRALIEQNEQLKRMATTDALTGLVNRRYLA
DMAGKVSGNFALILFDIDDFKTINDQYGHHVGDEVLVRIAEITRRVIGQHGYCARWGGEEFLILLPNFDAKRAYDLAACI
QDEFNESKGTLGAYHFTASFGVACNQGALSFHHVIRQADAAMYQGKKSGKNRVVSSSAMESREI
>Mature_304_residues
MKVEPSNTEILVVDDTVDNVKLLSQLLQNEGYLVKGALSAKSALRMCQKKKPDLILLDIMMPEVDGYELCRQLKKDPSTQ
DIPVIFLSALDHVQDKVRAFQIGGVDYIQKPFEVMEVLARVRTHAGLVTLQRALIEQNEQLKRMATTDALTGLVNRRYLA
DMAGKVSGNFALILFDIDDFKTINDQYGHHVGDEVLVRIAEITRRVIGQHGYCARWGGEEFLILLPNFDAKRAYDLAACI
QDEFNESKGTLGAYHFTASFGVACNQGALSFHHVIRQADAAMYQGKKSGKNRVVSSSAMESREI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=212, Percent_Identity=34.4339622641509, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=34.9693251533742, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=30.6010928961749, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=33.695652173913, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=37.1681415929204, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=32.7380952380952, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=138, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=36.2068965517241, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=35.042735042735, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=33.0434782608696, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=37.3913043478261, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=140, Percent_Identity=32.1428571428571, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=32.1637426900585, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=32.5714285714286, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=182, Percent_Identity=33.5164835164835, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=36.5217391304348, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=30.7228915662651, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790437, Length=108, Percent_Identity=36.1111111111111, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081977, Length=195, Percent_Identity=31.2820512820513, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=34.8623853211009, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787375, Length=124, Percent_Identity=32.258064516129, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790552, Length=114, Percent_Identity=30.7017543859649, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=30.0813008130081, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=30.635838150289, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33936; Mature: 33936

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEPSNTEILVVDDTVDNVKLLSQLLQNEGYLVKGALSAKSALRMCQKKKPDLILLDIM
CCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEE
MPEVDGYELCRQLKKDPSTQDIPVIFLSALDHVQDKVRAFQIGGVDYIQKPFEVMEVLAR
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHCHHHHHHHHHH
VRTHAGLVTLQRALIEQNEQLKRMATTDALTGLVNRRYLADMAGKVSGNFALILFDIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
KTINDQYGHHVGDEVLVRIAEITRRVIGQHGYCARWGGEEFLILLPNFDAKRAYDLAACI
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHH
QDEFNESKGTLGAYHFTASFGVACNQGALSFHHVIRQADAAMYQGKKSGKNRVVSSSAME
HHHHCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SREI
HCCC
>Mature Secondary Structure
MKVEPSNTEILVVDDTVDNVKLLSQLLQNEGYLVKGALSAKSALRMCQKKKPDLILLDIM
CCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEE
MPEVDGYELCRQLKKDPSTQDIPVIFLSALDHVQDKVRAFQIGGVDYIQKPFEVMEVLAR
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHCHHHHHHHHHH
VRTHAGLVTLQRALIEQNEQLKRMATTDALTGLVNRRYLADMAGKVSGNFALILFDIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
KTINDQYGHHVGDEVLVRIAEITRRVIGQHGYCARWGGEEFLILLPNFDAKRAYDLAACI
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHH
QDEFNESKGTLGAYHFTASFGVACNQGALSFHHVIRQADAAMYQGKKSGKNRVVSSSAME
HHHHCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SREI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA