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The map label for this gene is dsbE [H]

Identifier: 27366299

GI number: 27366299

Start: 3095540

End: 3096061

Strand: Reverse

Name: dsbE [H]

Synonym: VV1_3030

Alternate gene names: 27366299

Gene position: 3096061-3095540 (Counterclockwise)

Preceding gene: 27366300

Following gene: 27366296

Centisome position: 94.34

GC content: 41.0

Gene sequence:

>522_bases
GTGAACAACAAAGTTACTAAGTTGCTTGTGTTGGTGAGCATCGTCGGCATCGTTACGTTTACCGCAGTCTTGGGCATTGA
ACGACAAAATGCTGGTCACACAACGCCTGACACTCTGATTCCCTTGCCCAATCTTGAAGCAAAAGATCTTTTTTCGGCGA
ATACGATCACAACGCAAGATCTTATTTCCGATCAATACCGTGTGATGAACGTTTGGGCATCTTGGTGTGGGATTTGTCGG
GATGAACATACGTTTATTATGTCGCTTAAACAGCAGGGTATTGACGTGATTGGCTTAAATTATCGTGATAATAAGCAAGA
AGCGTTGGGCTATTTGGAGAGTGAAGGTAACCCTTATTCCAAGGTGATTTATGATCCAGACGGGATCGTCTCAATCGATT
TTGGCGTGATTGGCACACCAGAAACCTACTTAATAAACCCTAATGGGGAAATAATGATCAAATTTCGAGGTAAATTAGAT
CAAAAACAGTGGAATAAACATTTTGCTACGTATTTTAAATAG

Upstream 100 bases:

>100_bases
GCTTACATTTGGTGGATCTGGCTAGGCGGTTTGATGGTTGTTGCTGCGGTGTTCACTGCATTACAGCCAGTTCAGCAACG
CGTTAAGGTGGAGCGCTACT

Downstream 100 bases:

>100_bases
AACATTATTATCGACATGGCTCGTCTTATTCGTCGCTTTTGTCTCTTTCTCTAGTCAGGCACAGAACGAGAATACAAGCT
CTAATCACGTTGATTTGTTT

Product: putative thiol:disulfide oxidoreductase

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MNNKVTKLLVLVSIVGIVTFTAVLGIERQNAGHTTPDTLIPLPNLEAKDLFSANTITTQDLISDQYRVMNVWASWCGICR
DEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYSKVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLD
QKQWNKHFATYFK

Sequences:

>Translated_173_residues
MNNKVTKLLVLVSIVGIVTFTAVLGIERQNAGHTTPDTLIPLPNLEAKDLFSANTITTQDLISDQYRVMNVWASWCGICR
DEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYSKVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLD
QKQWNKHFATYFK
>Mature_173_residues
MNNKVTKLLVLVSIVGIVTFTAVLGIERQNAGHTTPDTLIPLPNLEAKDLFSANTITTQDLISDQYRVMNVWASWCGICR
DEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYSKVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLD
QKQWNKHFATYFK

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=174, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 19517; Mature: 19517

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKVTKLLVLVSIVGIVTFTAVLGIERQNAGHTTPDTLIPLPNLEAKDLFSANTITTQD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCCHHH
LISDQYRVMNVWASWCGICRDEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYS
HHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCE
KVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLDQKQWNKHFATYFK
EEEECCCCEEEEECCEECCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNKVTKLLVLVSIVGIVTFTAVLGIERQNAGHTTPDTLIPLPNLEAKDLFSANTITTQD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCCHHH
LISDQYRVMNVWASWCGICRDEHTFIMSLKQQGIDVIGLNYRDNKQEALGYLESEGNPYS
HHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCE
KVIYDPDGIVSIDFGVIGTPETYLINPNGEIMIKFRGKLDQKQWNKHFATYFK
EEEECCCCEEEEECCEECCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA