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The map label for this gene is btuC

Identifier: 27366069

GI number: 27366069

Start: 2844497

End: 2845492

Strand: Reverse

Name: btuC

Synonym: VV1_2782

Alternate gene names: 27366069

Gene position: 2845492-2844497 (Counterclockwise)

Preceding gene: 326424074

Following gene: 326424073

Centisome position: 86.7

GC content: 53.41

Gene sequence:

>996_bases
ATGGAACTGCACCAACTGATAGCCCACAAAGAGAAGCGATGGCGAGCCACCTTATTGGTGATGTTGGCTGTGCTTTTGGT
TCTCTCTGCTCTCTATTTGATGGTGGGTGAAGTCTTTCTTTCTCCGTTTTCTTCCTTGAGCAGCTTTGAACACAAGTTGT
TGGTTGATCTGCGCCTGCCGCGACTGGTGGCGGCAATGGTGATTGGTGCGGCATTAGCGGTCTCGGGGGCGACGTTACAA
GTCTTGCTTGGCAATGTGCTTGCTGAACCTGGTGTGTTAGGCATCTCCGGCGGGGCAAGTTTGGCCATGGTGATTGCGCT
CTTTTTATTGCCAGTGATGCCGACGCCAACGGTGTTTATGCTGGCAGCGATCATTGGTGCCTTGGTCTTTACGTTGATTT
TGGTGGGGATGGCAAGAGCGATGCACTTGTCGACCTCGAGAATGCTCCTCGTGGGGGTGGCGCTCGGTATTTTGTCGGGC
GCGTTTGTCACTTGGGCCTTTTATTTCAGTGACGACCTCAGTTTGCGTCAACTGATGTATTGGCTAATGGGGAGCATTGG
CGGGGCAAGTTGGCACCATCATCTGGTGACACTCGTGTTGCTTCCGGTCTTGGTTTGGCTCTGTCTGCAGGGGCAAACCT
TGGACAAACTGATGCTCGGTGAAACGCACGCTGCGCAGCTGGGCGTTGATGTGCACAAGGTACGTTGGCGGCTGATTTTG
GCGATCTCCATCTTGATTGGCTGTGCGGTGGCCCTTGGCGGCATAATCAGTTTTGTTGGTTTGGTCGTGCCTCATTTGCT
GCGCATGGCATTTGGTTCCGAAAACCGATTCTTGTTGCCGCTGTCGGCGTTTGCCGGGGCGACGTTACTGGTCTTTTCTG
ATATTTGGGCAAGAACCTTATTGGATTCGGCAGAGCTGCCGCTGGGGGTGATGACCACCACCATTGGCGCGCCCATCTTT
ATCTGGATGCTGATAAGGAGCCACGATGCACATTAA

Upstream 100 bases:

>100_bases
TGCTTAAGAGCAACAAGAGAGCGAGTTTAATGGTTTATTTCTGAAAGGCTTAACGTTAGAGTTAAGCCTTTTGTTTAGCG
AGCGAATTGAATCATTGCCC

Downstream 100 bases:

>100_bases
TTATATTAGTGTCGGAAATCGGCTTTTACCCCTGTCTTTTCAATGCGCTGCAGGTGAAAAAGTGTATGTCGCGGGCCCAA
ACGGTTCTGGAAAAAGTACC

Product: vtamin B12-transporter permease

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MELHQLIAHKEKRWRATLLVMLAVLLVLSALYLMVGEVFLSPFSSLSSFEHKLLVDLRLPRLVAAMVIGAALAVSGATLQ
VLLGNVLAEPGVLGISGGASLAMVIALFLLPVMPTPTVFMLAAIIGALVFTLILVGMARAMHLSTSRMLLVGVALGILSG
AFVTWAFYFSDDLSLRQLMYWLMGSIGGASWHHHLVTLVLLPVLVWLCLQGQTLDKLMLGETHAAQLGVDVHKVRWRLIL
AISILIGCAVALGGIISFVGLVVPHLLRMAFGSENRFLLPLSAFAGATLLVFSDIWARTLLDSAELPLGVMTTTIGAPIF
IWMLIRSHDAH

Sequences:

>Translated_331_residues
MELHQLIAHKEKRWRATLLVMLAVLLVLSALYLMVGEVFLSPFSSLSSFEHKLLVDLRLPRLVAAMVIGAALAVSGATLQ
VLLGNVLAEPGVLGISGGASLAMVIALFLLPVMPTPTVFMLAAIIGALVFTLILVGMARAMHLSTSRMLLVGVALGILSG
AFVTWAFYFSDDLSLRQLMYWLMGSIGGASWHHHLVTLVLLPVLVWLCLQGQTLDKLMLGETHAAQLGVDVHKVRWRLIL
AISILIGCAVALGGIISFVGLVVPHLLRMAFGSENRFLLPLSAFAGATLLVFSDIWARTLLDSAELPLGVMTTTIGAPIF
IWMLIRSHDAH
>Mature_331_residues
MELHQLIAHKEKRWRATLLVMLAVLLVLSALYLMVGEVFLSPFSSLSSFEHKLLVDLRLPRLVAAMVIGAALAVSGATLQ
VLLGNVLAEPGVLGISGGASLAMVIALFLLPVMPTPTVFMLAAIIGALVFTLILVGMARAMHLSTSRMLLVGVALGILSG
AFVTWAFYFSDDLSLRQLMYWLMGSIGGASWHHHLVTLVLLPVLVWLCLQGQTLDKLMLGETHAAQLGVDVHKVRWRLIL
AISILIGCAVALGGIISFVGLVVPHLLRMAFGSENRFLLPLSAFAGATLLVFSDIWARTLLDSAELPLGVMTTTIGAPIF
IWMLIRSHDAH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane

COG id: COG4139

COG function: function code H; ABC-type cobalamin transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily

Homologues:

Organism=Escherichia coli, GI1788004, Length=292, Percent_Identity=47.6027397260274, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790740, Length=282, Percent_Identity=40.0709219858156, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1790741, Length=286, Percent_Identity=32.5174825174825, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786805, Length=297, Percent_Identity=34.6801346801347, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786347, Length=288, Percent_Identity=36.4583333333333, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786804, Length=306, Percent_Identity=30.3921568627451, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BTUC_VIBVU (Q8D927)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761597.1
- ProteinModelPortal:   Q8D927
- SMR:   Q8D927
- GeneID:   1179627
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2782
- HOGENOM:   HBG700016
- OMA:   GMARAMH
- ProtClustDB:   PRK03784
- BioCyc:   VVUL216895:VV1_2782-MONOMER
- HAMAP:   MF_01004
- InterPro:   IPR000522

Pfam domain/function: PF01032 FecCD

EC number: NA

Molecular weight: Translated: 35695; Mature: 35695

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdd5ed34)-; HASH(0xf568274)-; HASH(0xf5681cc)-; HASH(0x1003e01c)-; HASH(0x10bffe0c)-; HASH(0x10bc5db0)-; HASH(0xfc48214)-; HASH(0x10203c20)-; HASH(0x108a6afc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELHQLIAHKEKRWRATLLVMLAVLLVLSALYLMVGEVFLSPFSSLSSFEHKLLVDLRLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLVAAMVIGAALAVSGATLQVLLGNVLAEPGVLGISGGASLAMVIALFLLPVMPTPTVFM
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHH
LAAIIGALVFTLILVGMARAMHLSTSRMLLVGVALGILSGAFVTWAFYFSDDLSLRQLMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WLMGSIGGASWHHHLVTLVLLPVLVWLCLQGQTLDKLMLGETHAAQLGVDVHKVRWRLIL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHCCHHHHHHHHHHH
AISILIGCAVALGGIISFVGLVVPHLLRMAFGSENRFLLPLSAFAGATLLVFSDIWARTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
LDSAELPLGVMTTTIGAPIFIWMLIRSHDAH
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MELHQLIAHKEKRWRATLLVMLAVLLVLSALYLMVGEVFLSPFSSLSSFEHKLLVDLRLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLVAAMVIGAALAVSGATLQVLLGNVLAEPGVLGISGGASLAMVIALFLLPVMPTPTVFM
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHH
LAAIIGALVFTLILVGMARAMHLSTSRMLLVGVALGILSGAFVTWAFYFSDDLSLRQLMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WLMGSIGGASWHHHLVTLVLLPVLVWLCLQGQTLDKLMLGETHAAQLGVDVHKVRWRLIL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHCCHHHHHHHHHHH
AISILIGCAVALGGIISFVGLVVPHLLRMAFGSENRFLLPLSAFAGATLLVFSDIWARTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
LDSAELPLGVMTTTIGAPIFIWMLIRSHDAH
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA