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The map label for this gene is astE

Identifier: 326424074

GI number: 326424074

Start: 2845611

End: 2846639

Strand: Reverse

Name: astE

Synonym: NA

Alternate gene names: 326424074

Gene position: 2846639-2845611 (Counterclockwise)

Preceding gene: 27366071

Following gene: 27366069

Centisome position: 86.74

GC content: 46.45

Gene sequence:

>1029_bases
ATGACGAATTCTCTCTTTCGCCAATCGTTTTTGTCAGACACCCTAAATATGCAGCAATCTGTCGAGGCGAAAGCGACGAC
GTTAAGCAATGGGGTAAGATTGCAGCTGCATCAGCGTGGTGTGTTAGAAGTCATTCCGGCAAACAACAGCGCGGAAACAA
AAAATATCATTTTATCGTCAGGGATCCACGGTGATGAAACCGCACCGATGGAACTGATTGACAAGATTGTTCACGATATC
GAAACGGGCTTTCAAGATGTGCAAGCGCGTTGTTTATTTATCATCGCTCATCCAGAAGCGACCAATGCGCACACGCGTTT
TATTGAAGAAAACCTCAACCGTCTGTTTGATGAAAAAGAGCATCAGCCAAGCAAAGAGTTGGTGATTGCCGATCAACTGA
AGTTATTGGTGAAAGCGTTTTTCGATAATACGCCCGTAGAAAGCCGTTGGCACTTAGATTTGCATTGCGCGATACGAGCG
TCGAAGCACTATTCGTTCGCGATCAGTCCGAAAACACGCCATCCGACACGTTCAAAAGCGTTGGTCGATTTTGTCAACCA
CAGCCATGTCGAAGCGTTGCTGCTCTCTAACTCACCATCCAGTACGTTTAGTTGGTTTAGTGCAGAATACTATTCTGCTC
AAGCACTGACGATGGAGTTAGGCCGAGTCGCTCGCATTGGTGAGAATGAGTTAGAGCGCTTTACGGCGTTGGATTTGACC
ATGCGTGATTTAATCGCAGAAGTGACCCCAGAGCATTTGCCTAAGCCAGCCATTACTTATCGTGTTAGCCGCACCATTGT
TCGTTTGCATCAAGACTTCGATTTCAGATTTGACGATCAAGTGGAAAACTTTACCTCGTTTATGCACGGTGAAGTGTTTG
GCCACGATGGTGATAAGCCGTTGATGGCGAAGAATGACAATGAAGCGATTGTGTTCCCTAACCGTAACGTGGCGATTGGT
CAACGCGCCGCACTAATGGTTTGTGAGGTCAAAGCCCGTTTTGAAGACGATCAACTGGTTTACGATTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: Succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:
NA

Sequences:

>Translated_342_residues
MTNSLFRQSFLSDTLNMQQSVEAKATTLSNGVRLQLHQRGVLEVIPANNSAETKNIILSSGIHGDETAPMELIDKIVHDI
ETGFQDVQARCLFIIAHPEATNAHTRFIEENLNRLFDEKEHQPSKELVIADQLKLLVKAFFDNTPVESRWHLDLHCAIRA
SKHYSFAISPKTRHPTRSKALVDFVNHSHVEALLLSNSPSSTFSWFSAEYYSAQALTMELGRVARIGENELERFTALDLT
MRDLIAEVTPEHLPKPAITYRVSRTIVRLHQDFDFRFDDQVENFTSFMHGEVFGHDGDKPLMAKNDNEAIVFPNRNVAIG
QRAALMVCEVKARFEDDQLVYD
>Mature_341_residues
TNSLFRQSFLSDTLNMQQSVEAKATTLSNGVRLQLHQRGVLEVIPANNSAETKNIILSSGIHGDETAPMELIDKIVHDIE
TGFQDVQARCLFIIAHPEATNAHTRFIEENLNRLFDEKEHQPSKELVIADQLKLLVKAFFDNTPVESRWHLDLHCAIRAS
KHYSFAISPKTRHPTRSKALVDFVNHSHVEALLLSNSPSSTFSWFSAEYYSAQALTMELGRVARIGENELERFTALDLTM
RDLIAEVTPEHLPKPAITYRVSRTIVRLHQDFDFRFDDQVENFTSFMHGEVFGHDGDKPLMAKNDNEAIVFPNRNVAIGQ
RAALMVCEVKARFEDDQLVYD

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=321, Percent_Identity=32.398753894081, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_VIBVU (Q8D926)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761598.1
- ProteinModelPortal:   Q8D926
- SMR:   Q8D926
- GeneID:   1179628
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2783
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- ProtClustDB:   PRK05324
- BioCyc:   VVUL216895:VV1_2783-MONOMER
- BRENDA:   3.5.1.96
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 38889; Mature: 38758

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSLFRQSFLSDTLNMQQSVEAKATTLSNGVRLQLHQRGVLEVIPANNSAETKNIILSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCEEEEEC
GIHGDETAPMELIDKIVHDIETGFQDVQARCLFIIAHPEATNAHTRFIEENLNRLFDEKE
CCCCCCCCHHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
HQPSKELVIADQLKLLVKAFFDNTPVESRWHLDLHCAIRASKHYSFAISPKTRHPTRSKA
CCCCHHEEHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCEEEEECCCCCCCCHHHH
LVDFVNHSHVEALLLSNSPSSTFSWFSAEYYSAQALTMELGRVARIGENELERFTALDLT
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MRDLIAEVTPEHLPKPAITYRVSRTIVRLHQDFDFRFDDQVENFTSFMHGEVFGHDGDKP
HHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEECCCCCCC
LMAKNDNEAIVFPNRNVAIGQRAALMVCEVKARFEDDQLVYD
CEECCCCCEEEECCCCEECCCCCEEEEEEEHHCCCCCCEECC
>Mature Secondary Structure 
TNSLFRQSFLSDTLNMQQSVEAKATTLSNGVRLQLHQRGVLEVIPANNSAETKNIILSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCEEEEEC
GIHGDETAPMELIDKIVHDIETGFQDVQARCLFIIAHPEATNAHTRFIEENLNRLFDEKE
CCCCCCCCHHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
HQPSKELVIADQLKLLVKAFFDNTPVESRWHLDLHCAIRASKHYSFAISPKTRHPTRSKA
CCCCHHEEHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCEEEEECCCCCCCCHHHH
LVDFVNHSHVEALLLSNSPSSTFSWFSAEYYSAQALTMELGRVARIGENELERFTALDLT
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MRDLIAEVTPEHLPKPAITYRVSRTIVRLHQDFDFRFDDQVENFTSFMHGEVFGHDGDKP
HHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEECCCCCCC
LMAKNDNEAIVFPNRNVAIGQRAALMVCEVKARFEDDQLVYD
CEECCCCCEEEECCCCEECCCCCEEEEEEEHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA