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The map label for this gene is maiA [H]

Identifier: 27366053

GI number: 27366053

Start: 2828172

End: 2828840

Strand: Reverse

Name: maiA [H]

Synonym: VV1_2765

Alternate gene names: 27366053

Gene position: 2828840-2828172 (Counterclockwise)

Preceding gene: 27366054

Following gene: 326424068

Centisome position: 86.2

GC content: 47.83

Gene sequence:

>669_bases
ATGGAGACAAGTCTTAAACTCTATGGTTATTGGCGATCGTCTGCCGCCTATCGCGTGAGGATCTGCTTAAACCTGAAGCA
GCTCCGCTATGAAAACCACTCGATTCATTTAGTGAAAAATGGCGGAGAGCAGCATCTCGCGCATTATCATGCTCTGAACC
CCAATGAGCTGGTGCCCGTGTTGGTGGATGGGGATCTGGTACTCAATCAGTCATTGGCTATCATTCAGTATCTCGATGAT
AACTATTCATCGACTCAAGTGATCCCGTCTATGGGGCCACTCAAGTATCAAGCGTTGGCGTTGGCGCAAGACATCGCGAT
TGATGTCCATCCTCTGAATAATCTTCGGGTCCTGCAGTATCTTGAAGGCAGTTTGGAGGTGGATGAAGAGCAAAAACGTT
TGTGGGTGCATCATTGGATTCATATGGGTTTTAAAGCGGTGGAAGAGAAGCTATTGACTCACCGTAAACACTATGGCGAG
TGTGTTTTTAGCGTCAGTTCTAGTCCAAGTATCGTCGATATATGTTTGGTCCCTCAGGTGTATAACGCGCTCCGTTTTGG
CGTAAACATGACACCGTATCCAACCATCAATGCCGTCGTCGCCGCTTGTAATCAACTGCCCGCATTCGATCTCGCTAAGC
CCGAAAATCAGCCTGACGCTCAACTTTGA

Upstream 100 bases:

>100_bases
GGCGATTCTATTCGCTTAGAAATGTTTGATGCGCAAGGGCAATCTATTTTTGGTGCGATTGAACAACAAGTGAGTCGTTA
TCAGCATAAATGAGGTTGGT

Downstream 100 bases:

>100_bases
ATTTACAGGTGGCGTTGTGATTCCTTGTTGGTAGATGTGTCAATTCAAGACTACATTTACTTGCAAGGGATCACAATTAG
GGGGCGTTATGTTCCGTTCA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
METSLKLYGYWRSSAAYRVRICLNLKQLRYENHSIHLVKNGGEQHLAHYHALNPNELVPVLVDGDLVLNQSLAIIQYLDD
NYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQYLEGSLEVDEEQKRLWVHHWIHMGFKAVEEKLLTHRKHYGE
CVFSVSSSPSIVDICLVPQVYNALRFGVNMTPYPTINAVVAACNQLPAFDLAKPENQPDAQL

Sequences:

>Translated_222_residues
METSLKLYGYWRSSAAYRVRICLNLKQLRYENHSIHLVKNGGEQHLAHYHALNPNELVPVLVDGDLVLNQSLAIIQYLDD
NYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQYLEGSLEVDEEQKRLWVHHWIHMGFKAVEEKLLTHRKHYGE
CVFSVSSSPSIVDICLVPQVYNALRFGVNMTPYPTINAVVAACNQLPAFDLAKPENQPDAQL
>Mature_222_residues
METSLKLYGYWRSSAAYRVRICLNLKQLRYENHSIHLVKNGGEQHLAHYHALNPNELVPVLVDGDLVLNQSLAIIQYLDD
NYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQYLEGSLEVDEEQKRLWVHHWIHMGFKAVEEKLLTHRKHYGE
CVFSVSSSPSIVDICLVPQVYNALRFGVNMTPYPTINAVVAACNQLPAFDLAKPENQPDAQL

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=215, Percent_Identity=39.0697674418605, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI194440732, Length=214, Percent_Identity=32.7102803738318, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI22202622, Length=164, Percent_Identity=35.9756097560976, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556142, Length=215, Percent_Identity=40, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17551302, Length=216, Percent_Identity=33.7962962962963, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17510461, Length=214, Percent_Identity=36.9158878504673, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645375, Length=215, Percent_Identity=40.9302325581395, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355859, Length=215, Percent_Identity=40.9302325581395, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553325, Length=215, Percent_Identity=40.9302325581395, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21355857, Length=222, Percent_Identity=33.7837837837838, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 25170; Mature: 25170

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSLKLYGYWRSSAAYRVRICLNLKQLRYENHSIHLVKNGGEQHLAHYHALNPNELVPV
CCCCEEEEEEECCCCEEEEEEEEEHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEE
LVDGDLVLNQSLAIIQYLDDNYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQY
EECCCEEECCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHEEEEECCCCEEEEEH
LEGSLEVDEEQKRLWVHHWIHMGFKAVEEKLLTHRKHYGECVFSVSSSPSIVDICLVPQV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECHHH
YNALRFGVNMTPYPTINAVVAACNQLPAFDLAKPENQPDAQL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METSLKLYGYWRSSAAYRVRICLNLKQLRYENHSIHLVKNGGEQHLAHYHALNPNELVPV
CCCCEEEEEEECCCCEEEEEEEEEHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEE
LVDGDLVLNQSLAIIQYLDDNYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQY
EECCCEEECCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHEEEEECCCCEEEEEH
LEGSLEVDEEQKRLWVHHWIHMGFKAVEEKLLTHRKHYGECVFSVSSSPSIVDICLVPQV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECHHH
YNALRFGVNMTPYPTINAVVAACNQLPAFDLAKPENQPDAQL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]