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The map label for this gene is potD [H]

Identifier: 27365895

GI number: 27365895

Start: 2639691

End: 2640728

Strand: Reverse

Name: potD [H]

Synonym: VV1_2600

Alternate gene names: 27365895

Gene position: 2640728-2639691 (Counterclockwise)

Preceding gene: 27365896

Following gene: 27365892

Centisome position: 80.46

GC content: 43.93

Gene sequence:

>1038_bases
ATGAAAAAAGCACTGTATACCGGCGCATTGTGTGCTGCTACTTTTTTTTCAACTTCTTCATTTGCAGCAGATCAAGAACT
GTATTTCTACAACTGGTCTGAGTATATCCCAAGTGAAGTGTTAGAAGATTTCACTAAAGAAACGGGTATCAAAGTCATCT
ATTCAACCTACGAGTCTAACGAGAGCATGTACGCAAAGTTAAAGACTCAAGGCAGTGGCTATGATCTGGTCGTTCCTTCT
ACTTATTTTGTTTCTAAGATGCGTAAAGAAGGAATGCTACAAAAAGTGGATAAAGCCAAGCTGTCTCACTTTGCCGAACT
AGACGCGAACTATCTAGACAAGCCATTTGACCCAGGTAATGACTACTCTATTCCATACATTTGGGGGGCGACTGGTATTG
GTATCAATGTTGACATGCTGGACAAGAGCTCAGTAAAAAACTGGGGAGATCTTTGGGATACTCAGTGGGCTGGTCAATTG
ATGTTGATGGACGATGCACGTGAAGTGTTCCACATCGCGCTTTCAAAGCTGGGTTACTCGCCAAACACAACCAACCCTGA
CGAGATCAAAGCCGCGTATGAAGAGCTGAAAAAACTGATCCCGAATGTATTGGTGTTCAACTCAGATTTCCCTGCCAATC
CCTATCTAGCGGGTGAAGTTTCACTGGGTATGCTTTGGAATGGCTCTGCGTACATGGCCCGTCAAGAAGGTGCAAAAATT
GACATCATCTGGCCTGAGAAAGGTGCAATTTTCTGGATGGATAGCTTAGCCATTCCAGCGGGTGCGAAAAATACTGAAGC
CGCACACAAGATGATCGACTTCCTTCTACGCCCAGAAAATGCGGCAAAAATTGCGATGGAAATCGGTTATCCAACCCCTG
TGAAAACCGCTTACAAACTGCTCCCTAAAGAGTTTGCCGAAGATCAAAACATCTTCCCTCCACAATCTGTGATGGATAAT
GGTGTGTGGCAAGACGAAGTCGGTGAAGCCAGTGTACTTTACGATGAGTACTTCCAAAAACTCAAAGTAAATAACTAA

Upstream 100 bases:

>100_bases
AGCTGATATAATCCGAGGCTGTTTTTTCTGGCGATACCTCATCGTCAGTTTTTATATAACCACCTGCCCTTTAGGGCACG
CTGATATGAGCGGAACAGTA

Downstream 100 bases:

>100_bases
TTTTATTGGTTAAAAAGAAAAAGCGGCTGTGGCCGCTTTTTTTGATCCTTCTTTCAACGGGCATTATGCGCTTTCAACGT
TACTCTCTAAATGCGCAAGC

Product: ABC transporter periplasmic spermidine putrescine-binding protein potD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKKALYTGALCAATFFSTSSFAADQELYFYNWSEYIPSEVLEDFTKETGIKVIYSTYESNESMYAKLKTQGSGYDLVVPS
TYFVSKMRKEGMLQKVDKAKLSHFAELDANYLDKPFDPGNDYSIPYIWGATGIGINVDMLDKSSVKNWGDLWDTQWAGQL
MLMDDAREVFHIALSKLGYSPNTTNPDEIKAAYEELKKLIPNVLVFNSDFPANPYLAGEVSLGMLWNGSAYMARQEGAKI
DIIWPEKGAIFWMDSLAIPAGAKNTEAAHKMIDFLLRPENAAKIAMEIGYPTPVKTAYKLLPKEFAEDQNIFPPQSVMDN
GVWQDEVGEASVLYDEYFQKLKVNN

Sequences:

>Translated_345_residues
MKKALYTGALCAATFFSTSSFAADQELYFYNWSEYIPSEVLEDFTKETGIKVIYSTYESNESMYAKLKTQGSGYDLVVPS
TYFVSKMRKEGMLQKVDKAKLSHFAELDANYLDKPFDPGNDYSIPYIWGATGIGINVDMLDKSSVKNWGDLWDTQWAGQL
MLMDDAREVFHIALSKLGYSPNTTNPDEIKAAYEELKKLIPNVLVFNSDFPANPYLAGEVSLGMLWNGSAYMARQEGAKI
DIIWPEKGAIFWMDSLAIPAGAKNTEAAHKMIDFLLRPENAAKIAMEIGYPTPVKTAYKLLPKEFAEDQNIFPPQSVMDN
GVWQDEVGEASVLYDEYFQKLKVNN
>Mature_345_residues
MKKALYTGALCAATFFSTSSFAADQELYFYNWSEYIPSEVLEDFTKETGIKVIYSTYESNESMYAKLKTQGSGYDLVVPS
TYFVSKMRKEGMLQKVDKAKLSHFAELDANYLDKPFDPGNDYSIPYIWGATGIGINVDMLDKSSVKNWGDLWDTQWAGQL
MLMDDAREVFHIALSKLGYSPNTTNPDEIKAAYEELKKLIPNVLVFNSDFPANPYLAGEVSLGMLWNGSAYMARQEGAKI
DIIWPEKGAIFWMDSLAIPAGAKNTEAAHKMIDFLLRPENAAKIAMEIGYPTPVKTAYKLLPKEFAEDQNIFPPQSVMDN
GVWQDEVGEASVLYDEYFQKLKVNN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=65.1090342679128, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787078, Length=334, Percent_Identity=34.4311377245509, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38854; Mature: 38854

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALYTGALCAATFFSTSSFAADQELYFYNWSEYIPSEVLEDFTKETGIKVIYSTYESN
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCHHHHHHHHHHHCCCEEEEEECCCC
ESMYAKLKTQGSGYDLVVPSTYFVSKMRKEGMLQKVDKAKLSHFAELDANYLDKPFDPGN
CEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
DYSIPYIWGATGIGINVDMLDKSSVKNWGDLWDTQWAGQLMLMDDAREVFHIALSKLGYS
CCCCCEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
PNTTNPDEIKAAYEELKKLIPNVLVFNSDFPANPYLAGEVSLGMLWNGSAYMARQEGAKI
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECEEEEEEEECCCEEEECCCCCEE
DIIWPEKGAIFWMDSLAIPAGAKNTEAAHKMIDFLLRPENAAKIAMEIGYPTPVKTAYKL
EEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
LPKEFAEDQNIFPPQSVMDNGVWQDEVGEASVLYDEYFQKLKVNN
HHHHHCCCCCCCCCHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKALYTGALCAATFFSTSSFAADQELYFYNWSEYIPSEVLEDFTKETGIKVIYSTYESN
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCHHHHHHHHHHHCCCEEEEEECCCC
ESMYAKLKTQGSGYDLVVPSTYFVSKMRKEGMLQKVDKAKLSHFAELDANYLDKPFDPGN
CEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
DYSIPYIWGATGIGINVDMLDKSSVKNWGDLWDTQWAGQLMLMDDAREVFHIALSKLGYS
CCCCCEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
PNTTNPDEIKAAYEELKKLIPNVLVFNSDFPANPYLAGEVSLGMLWNGSAYMARQEGAKI
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECEEEEEEEECCCEEEECCCCCEE
DIIWPEKGAIFWMDSLAIPAGAKNTEAAHKMIDFLLRPENAAKIAMEIGYPTPVKTAYKL
EEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
LPKEFAEDQNIFPPQSVMDNGVWQDEVGEASVLYDEYFQKLKVNN
HHHHHCCCCCCCCCHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]