Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 27365896

GI number: 27365896

Start: 2640890

End: 2641921

Strand: Reverse

Name: potD [H]

Synonym: VV1_2601

Alternate gene names: 27365896

Gene position: 2641921-2640890 (Counterclockwise)

Preceding gene: 326424047

Following gene: 27365895

Centisome position: 80.5

GC content: 44.67

Gene sequence:

>1032_bases
ATGAAAAAATGGGCTACTTTATTAGCTGGTAGTGCATGTGCGCTTTCACTGTTTTCTGGTTCGGTTGCAGCGGAAGATAA
AGAATTGGTATTTATGAACTGGGGTCCTTACATCAACAGTAATATCCTAGAGCAATTTACGAAAGAAACAGGTATCAAGG
TGATTTACTCGACTTACGAGTCGAACGAAACCTTGTACGCCAAGTTGAAAACGCACAACCAAGGCTATGATCTGGTAGTA
CCTTCTACCTACTTCGTATCTAAGATGCGTGATGAAGGTATGTTGCAGAAGATTGATAAATCGAAGCTAACAAACTTCAA
AAACTTAGATAAGAACTATCTAGATAAACCTTACGATCCAAACAACGACTACTCTATCCCACACGTTGTTGCGATCACGG
GTCTTGCTGTGAACACCGATATGTACGATCCAAATGACTTCCAAAGCTGGGCAGACCTATGGAAACCTGAGCTTAAGGGC
CAAGTCATGTTGATGGACGACACTCGTGAAGTGTTCCATATCGCGCTACGCAAATTGGGTTATTCGGGTAACACCACCGA
TGAAAAACAAATCGATGAAGCGTACGCAGAACTGCAAAAGTTAATGCCAAACGTATTGGTATTCAACTCTGACAACCCAG
GCGCACCATACATGTCTGGCGAAGTTGGCGTTGGCATGCTTTGGAACGGCAGTGCGGCGGCAGCACAAAAAGAAGGTTTA
CCTCTAACGCTTGTTTTCCCTAAAGAAGGCGGCATTGGTTGGGTAGATAACTTTGCAATTAGTTCTGGTGCGAAAAACGT
GGATGCGGCGCACAAGATGATCGATTTCCTACTTCGTCCAGAAATTGCAGAGCAAATCTCTAACGACACGGGTTACTTGA
CCGCTGTTGCTGAGTCAAACGCGAAGTTCAAAGATGTTGCGCCACTGTTCCCGTCACAAGAAGATCTTGACCGCGTAGAG
TGGCAAGACGCCGTTGGTGACAAAACAGTGAAGTACGAAGACTACTTCATGAAGCTTAAAGCTGGCCAATAA

Upstream 100 bases:

>100_bases
GCCTTCTGGTTGTGTTTAAACGCGAAGCAGAATGGTGATTCACCATTCTGTTTTTTTATCGGAATGCCTTCGGGCAACGT
TTGGTTTGGAGCTAACGTAA

Downstream 100 bases:

>100_bases
TCAGCACAATTGCTTCATTACTCATCATATAGGCAGCGGAAAGCTGCCTATTTTTTTACTTAGCTGATATAATCCGAGGC
TGTTTTTTCTGGCGATACCT

Product: ABC transporter periplasmic spermidine putrescine-binding protein potD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKKWATLLAGSACALSLFSGSVAAEDKELVFMNWGPYINSNILEQFTKETGIKVIYSTYESNETLYAKLKTHNQGYDLVV
PSTYFVSKMRDEGMLQKIDKSKLTNFKNLDKNYLDKPYDPNNDYSIPHVVAITGLAVNTDMYDPNDFQSWADLWKPELKG
QVMLMDDTREVFHIALRKLGYSGNTTDEKQIDEAYAELQKLMPNVLVFNSDNPGAPYMSGEVGVGMLWNGSAAAAQKEGL
PLTLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISNDTGYLTAVAESNAKFKDVAPLFPSQEDLDRVE
WQDAVGDKTVKYEDYFMKLKAGQ

Sequences:

>Translated_343_residues
MKKWATLLAGSACALSLFSGSVAAEDKELVFMNWGPYINSNILEQFTKETGIKVIYSTYESNETLYAKLKTHNQGYDLVV
PSTYFVSKMRDEGMLQKIDKSKLTNFKNLDKNYLDKPYDPNNDYSIPHVVAITGLAVNTDMYDPNDFQSWADLWKPELKG
QVMLMDDTREVFHIALRKLGYSGNTTDEKQIDEAYAELQKLMPNVLVFNSDNPGAPYMSGEVGVGMLWNGSAAAAQKEGL
PLTLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISNDTGYLTAVAESNAKFKDVAPLFPSQEDLDRVE
WQDAVGDKTVKYEDYFMKLKAGQ
>Mature_343_residues
MKKWATLLAGSACALSLFSGSVAAEDKELVFMNWGPYINSNILEQFTKETGIKVIYSTYESNETLYAKLKTHNQGYDLVV
PSTYFVSKMRDEGMLQKIDKSKLTNFKNLDKNYLDKPYDPNNDYSIPHVVAITGLAVNTDMYDPNDFQSWADLWKPELKG
QVMLMDDTREVFHIALRKLGYSGNTTDEKQIDEAYAELQKLMPNVLVFNSDNPGAPYMSGEVGVGMLWNGSAAAAQKEGL
PLTLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISNDTGYLTAVAESNAKFKDVAPLFPSQEDLDRVE
WQDAVGDKTVKYEDYFMKLKAGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=348, Percent_Identity=57.4712643678161, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787078, Length=314, Percent_Identity=36.3057324840764, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38333; Mature: 38333

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWATLLAGSACALSLFSGSVAAEDKELVFMNWGPYINSNILEQFTKETGIKVIYSTYE
CCHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEC
SNETLYAKLKTHNQGYDLVVPSTYFVSKMRDEGMLQKIDKSKLTNFKNLDKNYLDKPYDP
CCCEEEEEEEECCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHCCHHHCCHHHCCCCCCC
NNDYSIPHVVAITGLAVNTDMYDPNDFQSWADLWKPELKGQVMLMDDTREVFHIALRKLG
CCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHC
YSGNTTDEKQIDEAYAELQKLMPNVLVFNSDNPGAPYMSGEVGVGMLWNGSAAAAQKEGL
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCC
PLTLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISNDTGYLTAVAESN
CEEEEEECCCCCCCHHCHHHCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEECCC
AKFKDVAPLFPSQEDLDRVEWQDAVGDKTVKYEDYFMKLKAGQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCC
>Mature Secondary Structure
MKKWATLLAGSACALSLFSGSVAAEDKELVFMNWGPYINSNILEQFTKETGIKVIYSTYE
CCHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEC
SNETLYAKLKTHNQGYDLVVPSTYFVSKMRDEGMLQKIDKSKLTNFKNLDKNYLDKPYDP
CCCEEEEEEEECCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHCCHHHCCHHHCCCCCCC
NNDYSIPHVVAITGLAVNTDMYDPNDFQSWADLWKPELKGQVMLMDDTREVFHIALRKLG
CCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHC
YSGNTTDEKQIDEAYAELQKLMPNVLVFNSDNPGAPYMSGEVGVGMLWNGSAAAAQKEGL
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCC
PLTLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISNDTGYLTAVAESN
CEEEEEECCCCCCCHHCHHHCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEECCC
AKFKDVAPLFPSQEDLDRVEWQDAVGDKTVKYEDYFMKLKAGQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]